Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,140,009	T→C	D535G (GAC→GGC)	asmA ←	suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,140,009	0	T	C	100.0%	185.7 / NA	52	D535G (GAC→GGC)	asmA	suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/34);  total (18/34)

GCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAA  >  NC_000913/2139864‑2140154                                                                                                                                                  |                                                                                                                                                  gcgcAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCa                                                                                                                                               <  1:952132/150‑1 (MQ=255)     aGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGttt                                                                                                                                          <  1:227286/151‑1 (MQ=255)     aGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGttt                                                                                                                                          <  1:299085/151‑1 (MQ=255)         aCTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGa                                                                                                                                        <  1:222046/149‑1 (MQ=255)            gATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTg                                                                                                                                    <  1:692323/150‑1 (MQ=255)               ccACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGcc                                                                                                                                  >  1:202167/1‑149 (MQ=255)               ccACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAg                                                                                                                                <  1:298266/151‑1 (MQ=255)                cACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGa                                                                                                                               <  1:592403/151‑1 (MQ=255)                 aCTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGcc                                                                                                                                  >  2:422105/1‑147 (MQ=255)                   ttGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTc                                                                                                                            >  2:508953/1‑151 (MQ=255)                    tGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGTGTGCCGCAGGTTTGATCTGCCAGATTc                                                                                                                            >  2:229108/1‑150 (MQ=255)                    tGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTc                                                                                                                            <  2:266368/150‑1 (MQ=255)                      cAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAAc                                                                                                                         <  1:861193/151‑1 (MQ=255)                       aGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACa                                                                                                                        <  2:899954/151‑1 (MQ=255)                            gTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGc                                                                                                                     <  1:333298/149‑1 (MQ=255)                              aaTTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCtt                                                                                                                  <  1:779664/150‑1 (MQ=255)                               aTTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTcc                                                                                                                >  1:524953/1‑151 (MQ=255)                                       gctgcCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTc                                                                                                          >  2:99670/1‑149 (MQ=255)                                          gcCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAgcgc                                                                                                     >  1:139983/1‑151 (MQ=255)                                            cAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGcc                                                                                                    >  2:113849/1‑150 (MQ=255)                                            cAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCa                                                                                                   <  1:403355/151‑1 (MQ=255)                                                  ccATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGcac                                                                                               >  2:693362/1‑149 (MQ=255)                                                   cATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGTGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAgg                                                                                            <  1:229137/151‑1 (MQ=255)                                                   cATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAgg                                                                                            <  1:272824/151‑1 (MQ=255)                                                    aTAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGc                                                                                           <  1:108172/151‑1 (MQ=255)                                                       aaCCCTCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGAt                                                                                        <  2:499689/151‑1 (MQ=255)                                                        aCCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGAtt                                                                                       >  1:507797/1‑151 (MQ=255)                                                            gCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGAcc                                                                                   <  1:567614/151‑1 (MQ=255)                                                              aGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCtt                                                                                 <  2:72354/151‑1 (MQ=255)                                                               gCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCtt                                                                                 <  2:98014/150‑1 (MQ=255)                                                                       ggCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCg                                                                         <  2:636411/150‑1 (MQ=255)                                                                         cGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGttt                                                                      <  2:304179/151‑1 (MQ=255)                                                                         cGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGttt                                                                      >  1:347935/1‑151 (MQ=255)                                                                                  cAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCac                                                              <  2:518835/150‑1 (MQ=255)                                                                                  cAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCa                                                               <  2:142950/149‑1 (MQ=255)                                                                                   aGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCacga                                                            <  1:164626/151‑1 (MQ=255)                                                                                                   tGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACg                                             <  2:533751/150‑1 (MQ=255)                                                                                                           ccGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCgg                                    <  2:702013/151‑1 (MQ=255)                                                                                                            cGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggt                                   >  1:834746/1‑151 (MQ=255)                                                                                                             gTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtg                                  >  2:126601/1‑151 (MQ=255)                                                                                                             gTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtg                                  <  1:791435/151‑1 (MQ=255)                                                                                                              ttCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtgg                                 >  2:664968/1‑151 (MQ=255)                                                                                                               tCCAGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCggtg                                  >  2:583420/1‑149 (MQ=255)                                                                                                                  aGCCACCCACGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTaaa                             <  1:794940/151‑1 (MQ=255)                                                                                                                          acGACCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCaa                     <  1:509008/151‑1 (MQ=255)                                                                                                                             aCCCGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGcc                  >  1:381756/1‑151 (MQ=255)                                                                                                                               ccGAATATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCcgc                >  2:200301/1‑151 (MQ=255)                                                                                                                                  aaTATCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTc             <  2:695512/151‑1 (MQ=255)                                                                                                                                     atCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCAc           <  2:124143/150‑1 (MQ=255)                                                                                                                                     atCAAACTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCAc           <  1:92135/150‑1 (MQ=255)                                                                                                                                          aCTGGGTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATc     >  1:335833/1‑151 (MQ=255)                                                                                                                                             gggTGCCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCaaa  <  1:889485/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GCGCAGTAACTGATCCACTTGCAGGCTGTAATTGAGTTGCTGCCAGTTGCCATAAACCCGCAGCGGTACTGGCGTTTCTTTCAGGAAATCAATCAGTTTGCTTTCCCCGTTCCAGCCACCCACGACCCGAATATCAAACTGGGTGTCGCAGGTTTGATCTGCCAGATTCAACATGCCTTCCCCTGTCAGCGCCAGCACAGGCGATTGACCTTGCATGTCGTTTAACGTCACGACGCCATCCTTCAACGTCAAATCGGTGGTAAAGCGGTCAAGCCGCGTCACGTTATCAAA  >  NC_000913/2139864‑2140154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑