Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,160,028	C→T	P548L (CCG→CTG)	mdtC →	multidrug efflux system, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,160,028	0	C	T	100.0%	214.8 / NA	57	P548L (CCG→CTG)	mdtC	multidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/33);  total (24/33)

CTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGT  >  NC_000913/2159886‑2160169                                                                                                                                               |                                                                                                                                              cTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACct                                                                                                                                       >  2:516418/1‑151 (MQ=255)   gCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAACCctt                                                                                                                                      <  2:139099/150‑1 (MQ=255)     gTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACcttctt                                                                                                                                   <  2:677846/151‑1 (MQ=255)        gTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTccc                                                                                                                                <  2:843429/151‑1 (MQ=255)          tttGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCg                                                                                                                               <  2:11930/150‑1 (MQ=255)            tGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCgg                                                                                                                              >  1:77521/1‑149 (MQ=255)              gTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGc                                                                                                                           >  1:878362/1‑150 (MQ=255)                  cATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGac                                                                                                                      <  1:87267/151‑1 (MQ=255)                       tGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTgg                                                                                                                 >  1:254094/1‑151 (MQ=255)                        ggTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGc                                                                                                                >  1:922109/1‑151 (MQ=255)                              ccTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTg                                                                                                          <  2:138628/151‑1 (MQ=255)                                tGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGAt                                                                                                        >  1:102346/1‑151 (MQ=255)                                 gCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATg                                                                                                       <  1:402402/151‑1 (MQ=255)                                 gCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATg                                                                                                       >  2:919406/1‑151 (MQ=255)                                     caaGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCg                                                                                                   <  2:403817/151‑1 (MQ=255)                                     caaGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCg                                                                                                   <  2:969639/151‑1 (MQ=255)                                            aCGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCa                                                                                            <  1:245505/151‑1 (MQ=255)                                                  aGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGa                                                                                      <  2:573708/151‑1 (MQ=255)                                                      aCTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCag                                                                                  <  1:945131/151‑1 (MQ=255)                                                           aaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTa                                                                              >  2:668639/1‑150 (MQ=255)                                                           aaTGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTa                                                                              >  2:336812/1‑150 (MQ=255)                                                             tGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTAttt                                                                           >  2:27558/1‑151 (MQ=255)                                                              gggTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTAttt                                                                           <  1:700594/150‑1 (MQ=255)                                                               ggTGCTCAATCATACCCGTCTGGTGGTCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATCGGCGGGATTCAGGCGGATCAGAGTATTTCg                                                                         <  2:356374/151‑1 (MQ=255)                                                                  gCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGttt                                                                      <  1:84652/151‑1 (MQ=255)                                                                  gCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGttt                                                                      <  2:251295/151‑1 (MQ=255)                                                                   cTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTc                                                                     >  1:152852/1‑151 (MQ=255)                                                                     cAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCa                                                                    <  1:353760/150‑1 (MQ=255)                                                                      aaTCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAgg                                                                  <  1:279479/151‑1 (MQ=255)                                                                      aaTCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAgg                                                                  >  2:75032/1‑151 (MQ=255)                                                                            aCCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATgc                                                            >  1:121654/1‑151 (MQ=255)                                                                             cccGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATgct                                                           <  2:128837/151‑2 (MQ=255)                                                                                gTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCgg                                                        <  2:575438/151‑1 (MQ=255)                                                                                  cTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCgg                                                        >  2:14984/1‑149 (MQ=255)                                                                                    ggtggGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTa                                                      <  2:672992/149‑1 (MQ=255)                                                                                          cGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGc                                                >  1:519165/1‑149 (MQ=255)                                                                                           gtggtgCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAgg                                             >  1:471902/1‑151 (MQ=255)                                                                                             ggtgCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGAt                                           <  1:444723/151‑1 (MQ=255)                                                                                                gctgctTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTc                                        <  1:807953/151‑1 (MQ=255)                                                                                                gctgctTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTc                                        >  1:947825/1‑151 (MQ=255)                                                                                                   gctTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATg                                     <  1:179957/151‑1 (MQ=255)                                                                                                     gagccgaaatggcgcTGGATGTGTGACTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGa                                    >  2:450402/12‑150 (MQ=255)                                                                                                       ggCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGaaa                                  >  1:349255/1‑150 (MQ=255)                                                                                                         cACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAAtt                               <  2:688431/151‑1 (MQ=255)                                                                                                          aCCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTa                              <  1:656614/151‑1 (MQ=255)                                                                                                          aCCATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTa                              <  1:445465/151‑1 (MQ=255)                                                                                                            cATTGCGCTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATc                            <  1:546204/151‑1 (MQ=255)                                                                                                                gcgcTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGt                         <  2:388131/150‑1 (MQ=255)                                                                                                                 cgcTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGa                       <  2:102329/151‑1 (MQ=255)                                                                                                                   cTGAATATCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACg                     >  1:427012/1‑151 (MQ=255)                                                                                                                       atatCTGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATcc                 <  2:612937/151‑1 (MQ=255)                                                                                                                            tGGCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGc              >  2:637302/1‑149 (MQ=255)                                                                                                                             ggCTGTATATCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCggta             <  2:927532/149‑3 (MQ=255)                                                                                                                                  tataTCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGGCACTGGCGTGTTGCTGGGCGGGATTCAGGCGGGTCAGAGAAATTCGTTTCAGGGGAAGCGCGGAATGTTGCAGGATTTCATGAAAATTATCCGTGCCGATCCGGAAGAGGAt       >  1:759627/1‑150 (MQ=255)                                                                                                                                   atatCTCGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATa      <  1:54661/150‑1 (MQ=255)                                                                                                                                      tctcGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGt  >  2:606697/1‑151 (MQ=255)                                                                                                                                      tctcGATCCTGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGt  >  2:836562/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                              CTGCGTGGTTTTGGTCGCATGTTGGTAGCCCTGCAACAAGGCTACGGCAAGTCACTAAAATGGGTGCTCAATCATACCCGTCTGGTGGGCGTGGTGCTGCTTGGCACCATTGCGCTGAATATCTGGCTGTATATCTCGATCCCGAAAACCTTCTTCCCGGAGCAGGACACTGGCGTGTTGATGGGCGGGATTCAGGCGGATCAGAGTATTTCGTTTCAGGCGATGCGCGGTAAGTTGCAGGATTTCATGAAAATTATCCGTGACGATCCGGCAGTGGATAATGT  >  NC_000913/2159886‑2160169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑