Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,223,301	T→C	F373L (TTC→CTC)	dld →	D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,223,301	0	T	C	100.0%	232.6 / NA	63	F373L (TTC→CTC)	dld	D‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/35);  total (28/35)

CCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGT  >  NC_000913/2223158‑2223446                                                                                                                                                |                                                                                                                                                  ccTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCa                                                                                                                                             <  2:235446/150‑1 (MQ=255) ccTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAg                                                                                                                                            <  1:123753/151‑1 (MQ=255)    tCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGcc                                                                                                                                          <  2:138381/150‑1 (MQ=255)     ccTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAt                                                                                                                                        <  2:895713/151‑1 (MQ=255)        gatgatTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCAttt                                                                                                                                      <  1:400769/150‑1 (MQ=255)             ttgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAccgcc                                                                                                                                >  1:258366/1‑151 (MQ=255)              tgatAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTAccgccg                                                                                                                               >  2:320066/1‑151 (MQ=255)                atAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCcgcg                                                                                                                             <  1:759713/151‑1 (MQ=255)                atAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCcgcg                                                                                                                             >  1:346265/1‑151 (MQ=255)                  aaGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCcgcg                                                                                                                             <  2:883251/149‑1 (MQ=255)                     ttAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATg                                                                                                                         >  1:157646/1‑150 (MQ=255)                       aGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGaa                                                                                                                       >  1:20354/1‑150 (MQ=255)                          cACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAAc                                                                                                                   >  1:29950/1‑151 (MQ=255)                            ccGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTg                                                                                                                 <  1:152759/151‑1 (MQ=255)                                     tGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATa                                                                                                         >  2:907028/1‑150 (MQ=255)                                       ccGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAAt                                                                                                      <  2:712929/151‑1 (MQ=255)                                              tcttTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGc                                                                                                <  2:500087/150‑1 (MQ=255)                                              tcttTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGc                                                                                                >  2:103314/1‑150 (MQ=255)                                                        cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATctgctg                                                                                     <  2:653603/151‑1 (MQ=255)                                                        cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATctgctg                                                                                     >  2:290951/1‑151 (MQ=255)                                                        cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATctgct                                                                                      <  2:610487/150‑1 (MQ=255)                                                        cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATctg                                                                                        <  1:715458/148‑1 (MQ=255)                                                        cAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATct                                                                                         <  2:90174/147‑1 (MQ=255)                                                         aaGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGt                                                                                    >  1:687520/1‑151 (MQ=255)                                                          aGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGtt                                                                                   >  2:143816/1‑151 (MQ=255)                                                          aGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGtt                                                                                   <  2:610453/151‑1 (MQ=255)                                                            ggACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTaa                                                                                 <  1:591476/151‑1 (MQ=255)                                                                    cgatgcgatgCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcg                                                                         >  1:879211/1‑151 (MQ=255)                                                                     gatgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgg                                                                        <  2:287885/151‑1 (MQ=255)                                                                     gatgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgg                                                                        <  1:127766/151‑1 (MQ=255)                                                                     gatgcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgg                                                                        <  2:860873/151‑1 (MQ=255)                                                                        gcgatgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATggcgggcg                                                                     <  2:716055/151‑1 (MQ=255)                                                                           atgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATg                                                                  >  1:421953/1‑151 (MQ=255)                                                                            tgcTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAACATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAGAATTGGCGGGCGATgg                                                                 >  1:589719/1‑151 (MQ=255)                                                                                 gagaAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTgg                                                            <  1:638579/151‑1 (MQ=255)                                                                                      aGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGaa                                                       <  1:215274/151‑1 (MQ=255)                                                                                      aGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGa                                                        >  1:534298/1‑150 (MQ=255)                                                                                           aaTTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTTCCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGcc                                                    <  1:889286/149‑1 (MQ=255)                                                                                             ttcttcCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCaaa                                                 >  2:103690/1‑150 (MQ=255)                                                                                              tcttcCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATc                                               >  1:452036/1‑151 (MQ=255)                                                                                                    gtccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCt                                         <  2:968556/151‑1 (MQ=255)                                                                                                      ccgCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtgg                                       <  1:50312/151‑1 (MQ=255)                                                                                                         cATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggggg                                    >  1:713453/1‑151 (MQ=255)                                                                                                           ttttACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCtggtgg                                    >  1:711159/1‑149 (MQ=255)                                                                                                            tttACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGat                                  <  2:20352/150‑1 (MQ=255)                                                                                                            tttACTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGat                                  <  2:593602/150‑1 (MQ=255)                                                                                                               aCTGACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGattat                               <  2:346221/150‑1 (MQ=255)                                                                                                                  gACCGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTc                            >  1:447043/1‑150 (MQ=255)                                                                                                                    ccGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCaaa                         <  1:907108/151‑1 (MQ=255)                                                                                                                     cGTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCaaa                         >  1:739792/1‑150 (MQ=255)                                                                                                                      gTGCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAAc                        >  1:323789/1‑150 (MQ=255)                                                                                                                        gCGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAgg                     >  1:51903/1‑151 (MQ=255)                                                                                                                         cGATGCAAAAATTCGGTCCCCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGTGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGc                    <  2:681644/151‑1 (MQ=255)                                                                                                                         cGATGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAgg                     <  2:29946/150‑1 (MQ=255)                                                                                                                           aTGCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTCAAACAGGCCg                  >  2:34647/1‑151 (MQ=255)                                                                                                                             gCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGaa                <  2:859816/151‑1 (MQ=255)                                                                                                                             gCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGa                 >  1:976501/1‑150 (MQ=255)                                                                                                                             gCAAAAATTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCCTGGCTGGTGGATTATTTCCAACAAGCCg                  >  1:934072/1‑149 (MQ=255)                                                                                                                                 aaaTTCGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCg            <  1:320105/151‑1 (MQ=255)                                                                                                                                      cGGTCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGAttt        <  2:662058/150‑1 (MQ=255)                                                                                                                                         tCACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCttt    >  1:665280/1‑151 (MQ=255)                                                                                                                                          cACCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCttt    <  2:433090/150‑1 (MQ=255)                                                                                                                                           aCCTGCTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGGGGATTATTTTAAACAGGCCGAAGGCGGTTTCTTTGt  >  2:79924/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                  CCTTCCTGATGATTGATAAGTTAGGCACCGACAAGATGCCGTTCTTCTTTAATCTCAAGGGACGCACCGATGCGATGCTGGAGAAAGTGAAATTCTTCCGTCCGCATTTTACTGACCGTGCGATGCAAAAATTCGGTCACCTGTTCCCCAGCCATTTACCGCCGCGCATGAAAAACTGGCGCGATAAATACGAGCATCATCTGCTGTTAAAAATGGCGGGCGATGGCGTGGGCGAAGCCAAATCGTGGCTGGTGGATTATTTCAAACAGGCCGAAGGCGATTTCTTTGT  >  NC_000913/2223158‑2223446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑