Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,665,669	A→G	T79A (ACA→GCA)	csiE →	stationary phase inducible protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,665,669	0	A	G	100.0%	176.9 / NA	47	T79A (ACA→GCA)	csiE	stationary phase inducible protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/35);  total (12/35)

CCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAC  >  NC_000913/2665526‑2665814                                                                                                                                                |                                                                                                                                                  ccaccaTGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCt                                                                                                                                            <  2:77867/151‑1 (MQ=255)   accaTGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTa                                                                                                                                          <  2:995341/151‑1 (MQ=255)    ccaTGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTa                                                                                                                                          >  2:345857/1‑150 (MQ=255)     caTGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAAc                                                                                                                                        <  2:107114/151‑1 (MQ=255)     caTGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAAc                                                                                                                                        <  1:892212/151‑1 (MQ=255)        ggCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTa                                                                                                                                          <  1:359911/146‑1 (MQ=255)        ggCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCa                                                                                                                                      <  1:849715/150‑1 (MQ=255)           aaCCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGt                                                                                                                                  >  2:868148/1‑151 (MQ=255)             ccTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGttt                                                                                                                                <  2:923651/151‑1 (MQ=255)                   gCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGttt                                                                                                                          <  2:3555/151‑1 (MQ=255)                       gCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTAttt                                                                                                                      <  2:365869/151‑1 (MQ=255)                       gCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTAttt                                                                                                                      <  1:246274/151‑1 (MQ=255)                           aaTGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGTTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACt                                                                                                                  >  2:278262/1‑151 (MQ=255)                                 gtgGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTac                                                                                                            <  2:150676/151‑1 (MQ=255)                                   ggATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTacg                                                                                                           <  1:186413/150‑1 (MQ=255)                                       gatgatATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTCGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTg                                                                                                      <  1:726778/151‑1 (MQ=255)                                           atatTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGttt                                                                                                  <  1:199485/151‑1 (MQ=255)                                               tGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGt                                                                                              >  2:25147/1‑151 (MQ=255)                                                         gATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGt                                                                                    <  2:67157/151‑1 (MQ=255)                                                         gATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTCTCCGTCTGTGTCCGt                                                                                    <  1:63402/151‑1 (MQ=255)                                                              cAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCAttt                                                                               <  2:762449/151‑1 (MQ=255)                                                               aGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCAttt                                                                               >  1:58138/1‑150 (MQ=255)                                                                             ggAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTc                                                                >  1:511765/1‑151 (MQ=255)                                                                                      gcgcTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGcc                                                       <  2:677339/151‑1 (MQ=255)                                                                                           atcatcAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGa                                                   <  1:831176/150‑1 (MQ=255)                                                                                             catcaACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGa                                                   >  1:892265/1‑148 (MQ=255)                                                                                              atcaACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAagag                                               <  1:380957/151‑1 (MQ=255)                                                                                                caACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATGTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGt                                              <  2:159046/150‑1 (MQ=255)                                                                                                     cacaCTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAAc                                          <  1:118728/149‑1 (MQ=255)                                                                                                           gacgacTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAgcg                                   <  2:330892/150‑1 (MQ=255)                                                                                                           gacgacTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAgcg                                   <  2:451595/150‑1 (MQ=255)                                                                                                            acgacTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAgcggc                                 <  1:672605/151‑1 (MQ=255)                                                                                                            acgacTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAgcggc                                 <  2:632701/151‑1 (MQ=255)                                                                                                               acTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGcgcg                              >  1:55109/1‑151 (MQ=255)                                                                                                                cTGGTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCgg                             >  1:247856/1‑151 (MQ=255)                                                                                                                  ggTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGaa                           <  2:633007/151‑1 (MQ=255)                                                                                                                  ggTTATGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGa                            <  2:410937/150‑1 (MQ=255)                                                                                                                      aTGACGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTg                        <  2:354762/150‑1 (MQ=255)                                                                                                                         aCGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGcgcg                    >  2:598922/1‑151 (MQ=255)                                                                                                                          cGGTAGCTACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGt                   <  2:960313/151‑1 (MQ=255)                                                                                                                                 tACCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt            >  1:518392/1‑151 (MQ=255)                                                                                                                                  aCCGGGTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt            <  2:132106/150‑1 (MQ=255)                                                                                                                                     gggTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTacga        <  1:310165/151‑1 (MQ=255)                                                                                                                                      ggTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTacgac       >  1:609852/1‑151 (MQ=255)                                                                                                                                      ggTTGAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTacgac       <  1:995115/151‑1 (MQ=255)                                                                                                                                          gAAGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGAt    <  1:927634/150‑1 (MQ=255)                                                                                                                                           aaGGTGCAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc  <  1:576697/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  CCACCATGGCAACCTTCAGCGAGCTTAATGGTGTGGATGATGATATTGCCAGTCTTGATATCAGCGAAACAGGACGGGAGATCCTGCGCTATCATCAACTCACACTGACGACTGGTTATGACGGTAGCTACCGGGTTGAAGGTACAGTGCTTAACCAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAC  >  NC_000913/2665526‑2665814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑