Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,812,356	T→C	G310G (GGT→GGC)	emrA →	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,812,356	0	T	C	100.0%	200.1 / NA	54	G310G (GGT→GGC)	emrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/24);  total (30/24)

CGCAGATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGTAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATAAA  >  NC_000913/2812208‑2812500                                                                                                                                                     |                                                                                                                                                 cGCAGATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCaa                                                                                                                                                <  1:190134/151‑1 (MQ=255)  gCAGATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAAc                                                                                                                                               >  1:583143/1‑151 (MQ=255)   cAGATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAGATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACt                                                                                                                                              >  1:448115/1‑151 (MQ=255)     gATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTgg                                                                                                                                            >  2:723772/1‑151 (MQ=255)          ccAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCGTCCAGCGCAAAATGCGACCGGCAACTGGATCa                                                                                                                                        <  2:19201/150‑1 (MQ=255)            aaTATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAg                                                                                                                                     <  2:269626/151‑1 (MQ=255)             atatGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGCGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAg                                                                                                                                     >  1:27453/1‑150 (MQ=255)               atGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTc                                                                                                                                   <  2:127015/150‑1 (MQ=255)               atGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCg                                                                                                                                  <  1:387186/151‑1 (MQ=255)                tGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGt                                                                                                                                 >  2:49829/1‑151 (MQ=255)                     aTCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGc                                                                                                                            >  1:894903/1‑151 (MQ=255)                        ggTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTc                                                                                                                         <  2:814367/151‑1 (MQ=255)                                 gTCACTATCACCACGGATATTTGCGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAGCTGGATCAAAGTCGTTCAGCGTCTGCCTGTGc                                                                                                                <  2:682839/151‑1 (MQ=255)                                    aCTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGt                                                                                                              >  1:545334/1‑150 (MQ=255)                                       aTCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCg                                                                                                          >  1:287272/1‑151 (MQ=255)                                          accacGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAAc                                                                                                       <  2:592167/151‑1 (MQ=255)                                          accacGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAAc                                                                                                       >  2:11311/1‑151 (MQ=255)                                           ccacGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACt                                                                                                      <  2:958750/151‑1 (MQ=255)                                              cGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTgg                                                                                                    <  2:48914/150‑1 (MQ=255)                                               ggATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGAc                                                                                                  >  2:688738/1‑151 (MQ=255)                                                gATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGAc                                                                                                  <  2:108696/150‑1 (MQ=255)                                                   atTTACGGCGATGATGTGAAGTACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAg                                                                                               <  1:705039/150‑1 (MQ=255)                                                     ttACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGaa                                                                                             >  1:382175/1‑150 (MQ=255)                                                     ttACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGa                                                                                              >  1:644430/1‑149 (MQ=255)                                                       aCGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACa                                                                                          <  1:648318/151‑1 (MQ=255)                                                               gatgTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAg                                                                                   <  2:622886/150‑1 (MQ=255)                                                                  gtgAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCaa                                                                                <  2:454320/150‑1 (MQ=255)                                                                    gAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAatat                                                                             >  1:53054/1‑151 (MQ=255)                                                                         acacCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCt                                                                        <  2:735343/151‑1 (MQ=255)                                                                              agTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGt                                                                    >  1:915169/2‑150 (MQ=255)                                                                                 aaaGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCg                                                                >  1:200249/1‑151 (MQ=255)                                                                                     tggttggtCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGttt                                                            >  2:957807/1‑151 (MQ=255)                                                                                      ggttggtCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTg                                                           >  2:482051/1‑151 (MQ=255)                                                                                        ttggtCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTc                                                         >  2:633222/1‑151 (MQ=255)                                                                                        ttgggCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTc                                                         >  1:561210/1‑151 (MQ=255)                                                                                                  tatGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGTGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTgg                                                 >  1:305471/1‑149 (MQ=255)                                                                                                   atGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTg                                               >  2:439095/1‑150 (MQ=255)                                                                                                     gggCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGc                                            <  1:343257/151‑1 (MQ=255)                                                                                                      ggCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCg                                           <  1:869309/151‑1 (MQ=255)                                                                                                        cacaGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTc                                         >  2:488917/1‑151 (MQ=255)                                                                                                            ggTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATa                                     <  2:583079/151‑1 (MQ=255)                                                                                                                 cgcgTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTATGACTGTGCGTATCGAACTGGACCAGATACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATa                                     >  2:587816/1‑146 (MQ=255)                                                                                                                    gTTCTCACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAAc                             >  1:974427/1‑151 (MQ=255)                                                                                                                      tctcACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCg                           >  1:229876/1‑151 (MQ=255)                                                                                                                       ctcACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGt                          >  1:470169/1‑151 (MQ=255)                                                                                                                       ctcACTGCTTCCAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGt                          >  2:330465/1‑151 (MQ=255)                                                                                                                                 ccAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAg                 <  2:818636/150‑1 (MQ=255)                                                                                                                                 ccAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAg                 <  2:894821/150‑1 (MQ=255)                                                                                                                                  cAGCGCAAAATGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGt               <  1:571524/151‑1 (MQ=255)                                                                                                                                        aaaaTGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGAGCGTATCGAACTGGACCAGAAAAAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACAACTAACCGTGACGGTCAGGTACTGGc         >  2:79788/1‑151 (MQ=255)                                                                                                                                         aaaTGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGc         >  2:44256/1‑150 (MQ=255)                                                                                                                                          aaTGCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCTCTAACCGTGACGGTCAGGTACTGGCaa       <  2:589408/151‑1 (MQ=255)                                                                                                                                             gCGACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAAt     >  1:729697/1‑150 (MQ=255)                                                                                                                                               gACCGGCAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATaaa  <  1:560244/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGCAGATTGCCAATATGCGTATCGGTCAGCCGGTCACTATCACCACGGATATTTACGGCGATGATGTGAAATACACCGGTAAAGTGGTTGGTCTGGATATGGGCACAGGTAGCGCGTTCTCACTGCTTCCAGCGCAAAATGCGACCGGTAACTGGATCAAAGTCGTTCAGCGTCTGCCTGTGCGTATCGAACTGGACCAGAAACAGCTGGAGCAATATCCGCTGCGTATCGGTTTGTCCACGCTGGTGAGCGTCAATACCACTAACCGTGACGGTCAGGTACTGGCAAATAAA  >  NC_000913/2812208‑2812500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑