Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,858,929	A→C	T613P (ACC→CCC)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,858,929	0	A	C	100.0%	288.4 / NA	75	T613P (ACC→CCC)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (40/35);  total (40/35)

TACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCACCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGAT  >  NC_000913/2858781‑2859075                                                                                                                                                     |                                                                                                                                                   taCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATcccc                                                                                                                                                  >  2:503949/1‑151 (MQ=255)            acacCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAAc                                                                                                                                         <  2:415065/149‑1 (MQ=255)             cacCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACAt                                                                                                                                       <  2:914344/150‑1 (MQ=255)             cacCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATg                                                                                                                                      <  1:477737/151‑1 (MQ=255)             cacCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATg                                                                                                                                      <  2:62532/151‑1 (MQ=255)               ccTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATggg                                                                                                                                    <  1:8577/151‑1 (MQ=255)               ccTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATggg                                                                                                                                    <  1:843545/151‑1 (MQ=255)                cTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATg                                                                                                                                      >  2:56380/1‑148 (MQ=255)                 tGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCg                                                                                                                                  >  1:150055/1‑151 (MQ=255)                   cccGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGt                                                                                                                                >  2:854585/1‑151 (MQ=255)                   cccGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGt                                                                                                                                <  2:716519/151‑1 (MQ=255)                      gACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTaa                                                                                                                              >  1:271717/1‑150 (MQ=255)                        ccTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAg                                                                                                                           <  2:907522/151‑1 (MQ=255)                        ccTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAg                                                                                                                           <  2:140382/151‑1 (MQ=255)                        ccTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAg                                                                                                                           <  2:520884/151‑1 (MQ=255)                            cATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTAcc                                                                                                                       >  1:966289/1‑151 (MQ=255)                             aTTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCt                                                                                                                      <  1:97904/151‑1 (MQ=255)                                 aTAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTatat                                                                                                                  >  1:389825/1‑151 (MQ=255)                                 aTAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTatat                                                                                                                  <  1:253885/151‑1 (MQ=255)                                   aaaCCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATgc                                                                                                                <  2:928895/151‑1 (MQ=255)                                   aaaCCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATgc                                                                                                                >  2:232472/1‑151 (MQ=255)                                    aaCCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATgcg                                                                                                               >  1:274152/1‑151 (MQ=255)                                      ccGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGcc                                                                                                             >  1:742914/1‑151 (MQ=255)                                       cGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGcc                                                                                                             >  1:560340/1‑150 (MQ=255)                                            aTTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCg                                                                                                       >  1:102339/1‑151 (MQ=255)                                               cGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCAc                                                                                                    >  2:57297/1‑151 (MQ=255)                                                gCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACt                                                                                                   <  1:904133/151‑1 (MQ=255)                                                gCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACt                                                                                                   >  2:108818/1‑151 (MQ=255)                                                     aCCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTg                                                                                              >  1:583861/1‑151 (MQ=255)                                                        gAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTgcg                                                                                            >  1:995101/1‑150 (MQ=255)                                                           ggTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGa                                                                                        <  2:719901/151‑1 (MQ=255)                                                            gTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGAt                                                                                       >  1:446473/1‑151 (MQ=255)                                                              cGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATgg                                                                                     >  2:94304/1‑151 (MQ=255)                                                              cGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATg                                                                                      <  2:156053/150‑1 (MQ=255)                                                                     cGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACAt                                                                              <  2:800321/151‑1 (MQ=255)                                                                     cGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACAt                                                                              >  2:25204/1‑151 (MQ=255)                                                                        tAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCgg                                                                           >  1:279857/1‑151 (MQ=255)                                                                        tAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCgg                                                                           >  1:453544/1‑151 (MQ=255)                                                                          gTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCa                                                                         <  1:194781/151‑1 (MQ=255)                                                                           ttGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGc                                                                          >  1:936401/1‑149 (MQ=255)                                                                             gAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGc                                                                       <  2:356964/150‑1 (MQ=255)                                                                             gAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCt                                                                      <  2:368126/151‑1 (MQ=255)                                                                              aaCAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCt                                                                      >  2:651379/1‑150 (MQ=255)                                                                               aCAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTatat                                                                                                                  <  1:288023/105‑1 (MQ=255)                                                                               aCAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTatat                                                                                                                  >  2:287990/1‑105 (MQ=255)                                                                                 aaGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGt                                                                  >  2:577772/1‑151 (MQ=255)                                                                                   gTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTAGCTATATGCGCCAGACCGCACGGATTGCGCTGATGGCCTACATCGGCAGCTATGTAc                                                                >  1:824932/1‑151 (MQ=255)                                                                                        gAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCgg                                                             <  1:422933/149‑1 (MQ=255)                                                                                             aGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaaa                                                       >  1:678905/1‑150 (MQ=255)                                                                                              gCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACaaaa                                                      >  2:601448/1‑150 (MQ=255)                                                                                                 aTTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTc                                                  <  2:398878/151‑1 (MQ=255)                                                                                                  tttATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCACACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTc                                                  <  1:6981/150‑1 (MQ=255)                                                                                                   ttATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCg                                                 <  2:870009/150‑1 (MQ=255)                                                                                                     aTCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCg                                                 >  1:671052/1‑148 (MQ=255)                                                                                                       cGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGAtt                                            >  2:645194/1‑151 (MQ=255)                                                                                                       cGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGAt                                             <  2:703634/150‑1 (MQ=255)                                                                                                          cAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTTCCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGa                                         <  2:643560/151‑1 (MQ=255)                                                                                                              ccGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTa                                     <  2:289913/151‑1 (MQ=255)                                                                                                               cGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTat                                    >  2:646861/1‑151 (MQ=255)                                                                                                                gCTGAATCTATCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTatc                                   >  1:846541/1‑151 (MQ=255)                                                                                                                        tGTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCAt                           >  1:683608/1‑151 (MQ=255)                                                                                                                         gTCGCCGCAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATc                          >  2:240001/1‑151 (MQ=255)                                                                                                                            gccgcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTTCCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCttt                       <  2:892487/151‑1 (MQ=255)                                                                                                                               gcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTTCCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTAc                     <  2:86115/150‑1 (MQ=255)                                                                                                                               gcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTAcc                    <  1:318031/151‑1 (MQ=255)                                                                                                                               gcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTAcc                    >  1:724762/1‑151 (MQ=255)                                                                                                                               gcAGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTAcc                    <  1:304664/151‑1 (MQ=255)                                                                                                                                 aGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCcg                  <  2:914237/151‑1 (MQ=255)                                                                                                                                 aGCGCCGCATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCcg                  >  1:21915/1‑151 (MQ=255)                                                                                                                                    gccgcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGt               >  1:459085/1‑151 (MQ=255)                                                                                                                                     ccgcATGTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGt               >  2:922107/1‑150 (MQ=255)                                                                                                                                           gTTGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGcgcg        >  1:846190/1‑151 (MQ=255)                                                                                                                                            ttGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCgg       >  1:354833/1‑151 (MQ=255)                                                                                                                                             tGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCgg       <  1:836853/150‑1 (MQ=255)                                                                                                                                             tGATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGc      >  2:711875/1‑151 (MQ=255)                                                                                                                                              gATCATCCCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCa     <  2:806628/151‑1 (MQ=255)                                                                                                                                                 catccCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGc      >  2:753593/1‑147 (MQ=255)                                                                                                                                                 catccCCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGAt  <  2:519122/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGAACAAGTACTGAATGAGCCATTTATCGCCAACCCGCTGAATCTGTCGCCGCAGCGCCGCATGTTGATCATCACCGGTCCGAACATGGGCGGTAAAAGTACCTATATGCGCCAGACCGCACTGATTGCGCTGATGGCCTACATCGGCAGCTATGTACCGGCACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGAT  >  NC_000913/2858781‑2859075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑