Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,913,035 A→G L206P (CTC→CCC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,913,0350AG100.0% 188.1 / NA 48L206P (CTC→CCC) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

GTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGA  >  NC_000913/2912887‑2913175
                                                                                                                                                    |                                                                                                                                            
gtgtTTCGGTCGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGgggg                                                                                                                                            <  1:52436/151‑1 (MQ=255)
      cGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACgg                                                                                                                                      >  2:595742/1‑151 (MQ=255)
           gacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCa                                                                                                                                 <  2:75208/151‑1 (MQ=255)
               acacacTTCCGCTCTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGGCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTa                                                                                                                              <  2:696020/148‑1 (MQ=255)
                 acacTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAAt                                                                                                                            <  1:461101/150‑1 (MQ=255)
                 acacTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATc                                                                                                                           >  2:752915/1‑151 (MQ=255)
                     ttCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTcc                                                                                                                       >  2:150934/1‑151 (MQ=255)
                       ccGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCa                                                                                                                      <  2:339729/150‑1 (MQ=255)
                              aaCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCa                                                                                                               <  2:659972/150‑1 (MQ=255)
                              aaCGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCAt                                                                                                              >  2:714464/1‑151 (MQ=255)
                                     tCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGt                                                                                                        <  1:685557/150‑1 (MQ=255)
                                           ttCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCg                                                                                                  <  1:545427/150‑1 (MQ=255)
                                             cATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGAtt                                                                                               <  1:165380/151‑1 (MQ=255)
                                             cATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGAtt                                                                                               <  1:700063/151‑1 (MQ=255)
                                             cATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGAtt                                                                                               >  2:226974/1‑151 (MQ=255)
                                               tctcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTcc                                                                                             >  1:567046/1‑151 (MQ=255)
                                                  cAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTc                                                                                              <  2:696032/147‑1 (MQ=255)
                                                      gcgcAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACgg                                                                                      >  1:205252/1‑151 (MQ=255)
                                                              gACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCa                                                                               <  2:913600/150‑1 (MQ=255)
                                                                   aCGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg                                                                         >  1:43543/1‑151 (MQ=255)
                                                                     gAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCg                                                                       >  2:325399/1‑151 (MQ=255)
                                                                         tctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGt                                                                      >  1:174400/1‑148 (MQ=255)
                                                                            tCGATGTAGTGTTCGCGGTCGGGGCGACGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAGGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATg                                                                 <  1:622906/150‑1 (MQ=255)
                                                                            tCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATg                                                                 <  1:33476/150‑1 (MQ=255)
                                                                               aTGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTgg                                                             >  1:49625/1‑151 (MQ=255)
                                                                                    gtgtTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTacac                                                        >  2:31813/1‑151 (MQ=255)
                                                                                           cgGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGGGCGTAGATGTTGGTACACTCTTTTg                                                 >  2:283339/1‑151 (MQ=255)
                                                                                            ggTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTgc                                                >  2:118798/1‑151 (MQ=255)
                                                                                                gagaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTgccgcc                                            >  1:296082/1‑151 (MQ=255)
                                                                                                gagaCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTgccg                                              <  1:773866/149‑1 (MQ=255)
                                                                                                       cGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTacac                                     >  1:562328/1‑151 (MQ=255)
                                                                                                       cGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTacac                                     >  1:354122/1‑151 (MQ=255)
                                                                                                              gcagcaGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCAt                               <  1:382294/150‑1 (MQ=255)
                                                                                                              gcagcaGTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATc                              <  1:280256/151‑1 (MQ=255)
                                                                                                                 gcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTc                           <  1:246564/151‑1 (MQ=255)
                                                                                                                      ttGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGgc                       >  1:159271/1‑150 (MQ=255)
                                                                                                                       tGGCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGgcg                      <  2:844310/150‑1 (MQ=255)
                                                                                                                        ggCAATTCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGgcgc                     <  1:390481/150‑1 (MQ=255)
                                                                                                                             ttCGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCttt               >  2:558044/1‑151 (MQ=255)
                                                                                                                               cGTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCtttt              >  1:832056/1‑150 (MQ=255)
                                                                                                                                gTTTGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTAc            >  2:589765/1‑151 (MQ=255)
                                                                                                                                  ttGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACtt          >  1:68015/1‑151 (MQ=255)
                                                                                                                                   tGTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTc         >  1:845374/1‑151 (MQ=255)
                                                                                                                                    gTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTcg        <  2:758507/151‑1 (MQ=255)
                                                                                                                                    gTATTCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTc         <  1:283373/150‑1 (MQ=255)
                                                                                                                                        tCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCgcgc     >  2:553254/1‑150 (MQ=255)
                                                                                                                                        tCGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCgcgc     <  1:660968/150‑1 (MQ=255)
                                                                                                                                         cGGTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCgcgc     >  2:710584/1‑149 (MQ=255)
                                                                                                                                          ggTTGGATGGGGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGa  <  1:865183/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGA  >  NC_000913/2912887‑2913175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: