Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,356,384	T→C	S554P (TCG→CCG)	gltB →	glutamate synthase, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,356,384	0	T	C	100.0%	199.4 / NA	53	S554P (TCG→CCG)	gltB	glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/25);  total (28/25)

ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAA  >  NC_000913/3356238‑3356528                                                                                                                                                   |                                                                                                                                                 actTCCGCCAGCAGTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCg                                                                                                                                                <  1:591241/149‑1 (MQ=255)  ctcccGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCg                                                                                                                                                <  2:456999/145‑1 (MQ=255)        ccAGCATTTTGCCGGGGTGATTACCCCGCCAATCGCCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCCCCGTTTAAGCTTTAAACCGCCGATTCTg                                                                                                                                       <  1:768304/151‑1 (MQ=255)               tttCCCCGGGTGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTGGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTAc                                                                                                                                 <  2:171896/150‑1 (MQ=255)                 tGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTcc                                                                                                                              >  2:644040/1‑151 (MQ=255)                    ccgggtaacaaccccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAt                                                                                                                           <  1:974045/139‑1 (MQ=255)                    ccAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAt                                                                                                                           >  1:636054/1‑151 (MQ=255)                     cggttGACAACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATTCCGCCCGCCACCATTATCGGTCGTAAAATGAATGTCTTGTGCGAAGCAGAGGGCCAGGCCCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAtt                                                                                                                          <  1:662528/147‑1 (MQ=255)                     cAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAtt                                                                                                                          >  2:6033/1‑151 (MQ=255)                      aGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAttt                                                                                                                         >  2:256085/1‑151 (MQ=255)                       gttGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAttt                                                                                                                         <  1:280714/148‑1 (MQ=255)                       ggtcacaaccccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGAtt                                                                                                                          <  2:276519/140‑1 (MQ=255)                        gggacaaccccGCCAATCGACCCGCTGCGTGAAGCACATGTTATGTCGCGCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTGGGGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTc                                                                                                                        <  2:792289/142‑1 (MQ=255)                              accccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACa                                                                                                                   <  1:854156/147‑1 (MQ=255)                              accccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAg                                                                                                                  <  1:639924/148‑1 (MQ=255)                               aCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTACGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCt                                                                                                                >  1:98214/1‑151 (MQ=255)                                  cGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCac                                                                                                             <  1:453047/151‑1 (MQ=255)                                   gCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCacg                                                                                                            >  1:264535/1‑151 (MQ=255)                                         cGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATg                                                                                                      >  2:471343/1‑151 (MQ=255)                                            cccGTTGCGTGAAGCGCATGTTATGCCCCTCGCCACCAGTATGGGTCTTGAAATGACCGTCTTTTGCGAAGCAGAGGGCCGGGCGCCCCGTTTAAGCTTAAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGAt                                                                                                       <  2:358456/147‑1 (MQ=255)                                            cccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGa                                                                                                     <  1:448807/149‑1 (MQ=255)                                              cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGaa                                                                                                    >  2:180607/1‑148 (MQ=255)                                              cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGaa                                                                                                    >  2:869685/1‑148 (MQ=255)                                                   cGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGGCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGc                                                                                            >  2:992738/1‑151 (MQ=255)                                                         gcgcATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTAcc                                                                                      <  1:589780/151‑1 (MQ=255)                                                              tGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCgcgc                                                                                  >  1:795431/1‑150 (MQ=255)                                                               gTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCgcgc                                                                                  >  1:333209/1‑149 (MQ=255)                                                                 tATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGa                                                                               <  1:971860/150‑1 (MQ=255)                                                                        ctcgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTgg                                                                       >  1:444560/1‑151 (MQ=255)                                                                          cgcCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGat                                                                     >  1:742059/1‑151 (MQ=255)                                                                            ccaccaGTATCGGTCGTGAAATGAACGTTTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGata                                                                    <  1:802233/150‑1 (MQ=255)                                                                             caccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATc                                                                  >  1:395884/1‑151 (MQ=255)                                                                             aaccaGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATc                                                                  >  1:660256/2‑151 (MQ=255)                                                                                caGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCAcc                                                               >  1:14247/1‑151 (MQ=255)                                                                                   tATCGGTCGTGAAATGACCGTCTTTTGGGAAGCAGAGGGCCAGGCGCCCCTTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCtt                                                             <  1:293056/150‑1 (MQ=255)                                                                                    aTCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTg                                                           >  1:368800/1‑151 (MQ=255)                                                                                          cGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTc                                                      >  1:506250/1‑150 (MQ=255)                                                                                             gAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACt                                                   >  1:652297/1‑150 (MQ=255)                                                                                                        cTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTc                                       >  1:762673/1‑151 (MQ=255)                                                                                                         ttttGCGAAGCAGAGGGCCAGGCGCACCTTTTAAGCTTAAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCt                                        <  2:944147/149‑1 (MQ=255)                                                                                                            tGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGa                                     >  1:548252/1‑149 (MQ=255)                                                                                                                aaGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGAc                               >  1:171195/1‑151 (MQ=255)                                                                                                                    agagGGCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACAATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTc                           >  1:340704/1‑151 (MQ=255)                                                                                                                       gggCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCaaa                        <  2:811245/151‑1 (MQ=255)                                                                                                                        ggCCAGGCGCACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAag                       <  1:274908/151‑1 (MQ=255)                                                                                                                              gcgcACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTGCCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCtgt                 >  1:644399/1‑151 (MQ=255)                                                                                                                                 cACCGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCg              >  1:140685/1‑151 (MQ=255)                                                                                                                                    cGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGAc            >  2:892005/1‑150 (MQ=255)                                                                                                                                    cGTTTAAGCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACa           <  1:231448/151‑1 (MQ=255)                                                                                                                                         aaGCTTAAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGc       <  1:402846/150‑1 (MQ=255)                                                                                                                                           gCTTTAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCg     >  2:283656/1‑150 (MQ=255)                                                                                                                                              ttAAACCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaaa  <  1:4991/150‑1 (MQ=255)                                                                                                                                              taaaaCCGACGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGaaa  <  2:974883/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATTTCAAACAGCTCACGACGATGAAAGAGGAGCACTACCGCGCAGATACGCTGGATATCACCTTTGACGTCACTAAAACCACGCTCGAAGCGACAGTCAAAGAGCTGTGCGACAAAGCCGAAA  >  NC_000913/3356238‑3356528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑