Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,928	C→T	A54T (GCA→ACA)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,928	0	C	T	100.0%	231.3 / NA	61	A54T (GCA→ACA)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/28);  total (33/28)

TGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCA  >  NC_000913/3400784‑3401070                                                                                                                                                 |                                                                                                                                               tGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTAc                                                                                                                                          >  2:491932/1‑151 (MQ=255)  gTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTa                                                                                                                                           <  1:820693/149‑1 (MQ=255)  gTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACg                                                                                                                                         <  1:429360/151‑1 (MQ=255)   ttGCTGCGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACTGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGc                                                                                                                                        >  2:671322/1‑151 (MQ=255)    tGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCt                                                                                                                                       >  2:494115/1‑151 (MQ=255)     gCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCtt                                                                                                                                      <  2:6980/151‑1 (MQ=255)      cTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCttt                                                                                                                                     >  1:631817/1‑151 (MQ=255)      cTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCttt                                                                                                                                     >  1:902279/1‑151 (MQ=255)        gtgCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTc                                                                                                                                   <  2:103422/151‑1 (MQ=255)          gCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCgg                                                                                                                                 <  2:169244/151‑1 (MQ=255)          gCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCgg                                                                                                                                 <  1:591960/151‑1 (MQ=255)              ttGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGa                                                                                                                              >  1:421967/1‑150 (MQ=255)               tGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAAc                                                                                                                            >  1:552381/1‑151 (MQ=255)                gTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAAcc                                                                                                                           >  1:5413/1‑151 (MQ=255)                   tgatgaAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCgg                                                                                                                         <  2:578270/150‑1 (MQ=255)                    gatgaAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCa                                                                                                                       <  2:753687/151‑1 (MQ=255)                     atgaAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCAc                                                                                                                      <  2:493857/151‑1 (MQ=255)                        aaGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGAt                                                                                                                   >  2:714682/1‑151 (MQ=255)                         aGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATc                                                                                                                  >  1:578676/1‑151 (MQ=255)                              tGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGAc                                                                                                             >  2:872653/1‑151 (MQ=255)                                   cATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATg                                                                                                        <  2:572691/151‑1 (MQ=255)                                   cATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATg                                                                                                        >  1:729946/1‑151 (MQ=255)                                     ttttttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGc                                                                                                      >  1:844974/1‑151 (MQ=255)                                       ttttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGcc                                                                                                     >  1:529358/1‑150 (MQ=255)                                        tttCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGccac                                                                                                   >  1:634369/1‑151 (MQ=255)                                           cAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGccacca                                                                                                 <  2:867313/150‑1 (MQ=255)                                               cACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGaa                                                                                            <  1:420042/151‑1 (MQ=255)                                               cACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGaa                                                                                            <  1:484379/151‑1 (MQ=255)                                               cACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGa                                                                                             <  1:977165/150‑1 (MQ=255)                                               cACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGa                                                                                             >  1:838836/1‑150 (MQ=255)                                               cACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGa                                                                                             >  2:690696/1‑150 (MQ=255)                                                      aagTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCAt                                                                                     >  2:4350/1‑151 (MQ=255)                                                      aagTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCAt                                                                                     >  2:929003/1‑151 (MQ=255)                                                       agTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCAtt                                                                                    <  2:781534/151‑1 (MQ=255)                                                             cGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATAc                                                                              >  2:951879/1‑151 (MQ=255)                                                                    gCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCt                                                                       >  1:455956/1‑151 (MQ=255)                                                                     ccGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTCCGCGTCATGACCTACTGTAGCTACGCCTTTCGGTGAACCCGCCCGATCCTGACAAATGGCCACCACCGAAGGCTCATTCAATACGATGCCtt                                                                      <  2:106792/151‑1 (MQ=255)                                                                           ttCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTcc                                                                  <  2:991395/149‑1 (MQ=255)                                                                             cATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCtttt                                                              <  2:240837/151‑1 (MQ=255)                                                                             cATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCtttt                                                              <  1:748200/151‑1 (MQ=255)                                                                                 gggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACa                                                           <  1:387082/150‑1 (MQ=255)                                                                                  ggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACAt                                                          >  1:479389/1‑150 (MQ=255)                                                                                    cGAATGGCAGCAATATTGACCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCAACACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATaa                                                        >  2:772413/1‑150 (MQ=255)                                                                                            agcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAGCCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAggg                                                 >  1:226090/1‑149 (MQ=255)                                                                                            agcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTa                                               <  1:69704/151‑1 (MQ=255)                                                                                             gcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAt                                              <  2:765548/151‑1 (MQ=255)                                                                                               aaTATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTc                                            <  2:53433/151‑1 (MQ=255)                                                                                                  atTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGc                                          >  2:57078/1‑150 (MQ=255)                                                                                                    tGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAg                                        <  2:225733/150‑1 (MQ=255)                                                                                                              aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTc                              >  2:274113/1‑150 (MQ=255)                                                                                                                  cccAGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATgg                         >  1:303583/1‑151 (MQ=255)                                                                                                                     aGCATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGAc                       >  1:683167/1‑150 (MQ=255)                                                                                                                       cATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACa                      >  2:37658/1‑149 (MQ=255)                                                                                                                       cATCTGCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACa                      >  1:404748/1‑149 (MQ=255)                                                                                                                            gCTTCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAt                <  2:166088/150‑1 (MQ=255)                                                                                                                              ttCGCGTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTgg             >  2:45783/1‑151 (MQ=255)                                                                                                                                 gcgTCATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGaa           >  2:926043/1‑150 (MQ=255)                                                                                                                                     cATGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACAt      <  1:714760/151‑1 (MQ=255)                                                                                                                                       tGACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGc    >  1:960518/1‑151 (MQ=255)                                                                                                                                        gACCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGcc   <  2:523828/151‑1 (MQ=255)                                                                                                                                         aCCTACTGTAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCa  <  2:545100/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               TGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCA  >  NC_000913/3400784‑3401070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑