Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,982	A→G	S6S (AGT→AGC)	rplQ ←	50S ribosomal subunit protein L17

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,982	0	A	G	100.0%	217.5 / NA	57	S6S (AGT→AGC)	rplQ	50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/27);  total (30/27)

TGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGT  >  NC_000913/3439834‑3440129                                                                                                                                                     |                                                                                                                                                    tGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGct                                                                                                                                                   >  1:410719/1‑151 (MQ=255)   gCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGctct                                                                                                                                                 <  1:833171/151‑1 (MQ=255)   gCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGctct                                                                                                                                                 >  1:346041/1‑151 (MQ=255)      agagTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTAc                                                                                                                                              <  1:300963/151‑1 (MQ=255)          tAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGa                                                                                                                                            >  2:300070/1‑149 (MQ=255)             tCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATg                                                                                                                                          >  1:826420/1‑148 (MQ=255)                   gCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGAc                                                                                                                                 <  1:91214/151‑1 (MQ=255)                       aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCtt                                                                                                                              >  1:976097/1‑150 (MQ=255)                          tACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATcc                                                                                                                          >  1:787303/1‑151 (MQ=255)                           aCGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATcc                                                                                                                          <  2:142233/150‑1 (MQ=255)                           aCGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTCCAGGACGAACCGGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATcc                                                                                                                          <  2:867567/150‑1 (MQ=255)                             gcggcgCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTtc                                                                                                                       <  1:832767/151‑1 (MQ=255)                               ggcgCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTtctc                                                                                                                     <  1:426950/151‑1 (MQ=255)                               ggcgCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTtct                                                                                                                      <  1:491673/150‑1 (MQ=255)                                  gcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGt                                                                                                                  <  1:457718/151‑1 (MQ=255)                                  gcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGt                                                                                                                  >  1:808656/1‑151 (MQ=255)                                   cAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGt                                                                                                                  >  2:237403/1‑150 (MQ=255)                                       tctTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAAc                                                                                                             >  2:867228/1‑151 (MQ=255)                                          ttCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCtt                                                                                                          >  2:512573/1‑151 (MQ=255)                                            cGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTa                                                                                                         <  1:295651/150‑1 (MQ=255)                                              cTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAcc                                                                                                      >  1:630541/1‑151 (MQ=255)                                              cTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAc                                                                                                       <  1:318442/150‑1 (MQ=255)                                                       gagTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATcc                                                                                              <  2:734040/150‑1 (MQ=255)                                                             tcTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACt                                                                                       >  1:633029/1‑151 (MQ=255)                                                                tgatgaTTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCg                                                                                     >  1:410195/1‑150 (MQ=255)                                                                   tgatTTCATGACGAACCAGTGACCCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTc                                                                                   <  2:31474/149‑1 (MQ=255)                                                                        tCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATg                                                                            >  1:277625/1‑151 (MQ=255)                                                                        tCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATg                                                                            >  2:112617/1‑151 (MQ=255)                                                                            gACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCtt                                                                         <  1:290588/150‑1 (MQ=255)                                                                            gACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTg                                                                        >  2:32651/1‑151 (MQ=255)                                                                             aCGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGc                                                                       >  1:611054/1‑151 (MQ=255)                                                                                   cAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTgg                                                                 >  1:580946/1‑151 (MQ=255)                                                                                    aGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTgg                                                                 >  2:261504/1‑150 (MQ=255)                                                                                        aaCCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGcta                                                              <  2:30315/149‑3 (MQ=255)                                                                                               cATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTcc                                                       >  1:537468/1‑149 (MQ=255)                                                                                               cATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAg                                                     <  1:301383/151‑1 (MQ=255)                                                                                                atatTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAgg                                                    <  2:642395/151‑1 (MQ=255)                                                                                                 tatTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGgc                                                   >  1:93110/1‑151 (MQ=255)                                                                                                   ttGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGgcgc                                                 >  2:125374/1‑151 (MQ=255)                                                                                                     gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCa                                                >  1:843688/1‑150 (MQ=255)                                                                                                     gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCAt                                               >  2:727623/1‑151 (MQ=255)                                                                                                     gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCAt                                               >  1:344970/1‑151 (MQ=255)                                                                                                        gAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGcc                                            <  2:213119/151‑1 (MQ=255)                                                                                                          aCATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCa                                          <  2:133130/151‑1 (MQ=255)                                                                                                            aTAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGccc                                           >  1:249264/1‑148 (MQ=255)                                                                                                                ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACa                                    <  2:787220/151‑1 (MQ=255)                                                                                                                ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACa                                    <  1:235444/151‑1 (MQ=255)                                                                                                                  tGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGt                                  >  2:302301/1‑151 (MQ=255)                                                                                                                              ctgTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGcc                      >  1:554361/1‑151 (MQ=255)                                                                                                                                gTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGATTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAg                    <  1:722411/151‑1 (MQ=255)                                                                                                                                  tGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGc                   <  2:250522/150‑1 (MQ=255)                                                                                                                                  tGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGc                   >  1:423641/1‑150 (MQ=255)                                                                                                                                   gCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCAc                 <  2:320077/151‑1 (MQ=255)                                                                                                                                     ggTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGt               <  1:185591/151‑1 (MQ=255)                                                                                                                                          aGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCttt           >  2:788746/1‑150 (MQ=255)                                                                                                                                              gacgacCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtct      <  2:99607/151‑1 (MQ=255)                                                                                                                                                  acCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGt  <  1:540720/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGT  >  NC_000913/3439834‑3440129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑