Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,068 C→T W321* (TGG→TAG)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,0680CT100.0% 227.8 / NA 59W321* (TGG→TAG) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/33);  total (26/33)

CATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCT  >  NC_000913/3439928‑3440214
                                                                                                                                            |                                                                                                                                                  
cATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTcc                                                                                                                                            >  1:537468/1‑149 (MQ=255)
cATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAg                                                                                                                                          <  1:301383/151‑1 (MQ=255)
 atatTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAgg                                                                                                                                         <  2:642395/151‑1 (MQ=255)
  tatTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGgc                                                                                                                                        >  1:93110/1‑151 (MQ=255)
    ttGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGgcgc                                                                                                                                      >  2:125374/1‑151 (MQ=255)
      gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCa                                                                                                                                     >  1:843688/1‑150 (MQ=255)
      gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCAt                                                                                                                                    >  2:727623/1‑151 (MQ=255)
      gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCAt                                                                                                                                    >  1:344970/1‑151 (MQ=255)
         gAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGcc                                                                                                                                 <  2:213119/151‑1 (MQ=255)
           aCATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCa                                                                                                                               <  2:133130/151‑1 (MQ=255)
             aTAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGccc                                                                                                                                >  1:249264/1‑148 (MQ=255)
                 ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACa                                                                                                                         <  1:235444/151‑1 (MQ=255)
                 ccTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACa                                                                                                                         <  2:787220/151‑1 (MQ=255)
                   tGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGt                                                                                                                       >  2:302301/1‑151 (MQ=255)
                               ctgTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGcc                                                                                                           >  1:554361/1‑151 (MQ=255)
                                 gTTGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGATTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAg                                                                                                         <  1:722411/151‑1 (MQ=255)
                                   tGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGc                                                                                                        >  1:423641/1‑150 (MQ=255)
                                   tGCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGc                                                                                                        <  2:250522/150‑1 (MQ=255)
                                    gCGGTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCAc                                                                                                      <  2:320077/151‑1 (MQ=255)
                                      ggTTCAGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGt                                                                                                    <  1:185591/151‑1 (MQ=255)
                                           aGTTGACGACCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCttt                                                                                                >  2:788746/1‑150 (MQ=255)
                                               gacgacCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtct                                                                                           <  2:99607/151‑1 (MQ=255)
                                                   acCGCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGt                                                                                       <  1:540720/151‑1 (MQ=255)
                                                    ccgctctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTa                                                                                      <  2:421160/148‑1 (MQ=255)
                                                     cgctctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTa                                                                                      >  1:178148/3‑150 (MQ=255)
                                                     cgctctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTa                                                                                      >  1:564704/3‑150 (MQ=255)
                                                      gctctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAag                                                                                    <  1:867306/150‑1 (MQ=255)
                                                       ctctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTa                                                                                      >  2:105734/1‑148 (MQ=255)
                                                                tGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtttttt                                                                           >  1:105791/1‑150 (MQ=255)
                                                                 ggCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTAc                                                                         <  2:971873/151‑1 (MQ=255)
                                                                    gcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCaa                                                                      <  2:410672/151‑1 (MQ=255)
                                                                      aTGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAgg                                                                    <  2:416327/151‑1 (MQ=255)
                                                                      aTGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAgg                                                                    >  2:769214/1‑151 (MQ=255)
                                                                            ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGc                                                              >  2:704976/1‑151 (MQ=255)
                                                                             tATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGc                                                              <  1:925234/150‑1 (MQ=255)
                                                                             tATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCg                                                             >  1:904473/1‑151 (MQ=255)
                                                                                   tctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                         <  2:118013/149‑1 (MQ=255)
                                                                                     tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                     >  1:548421/1‑151 (MQ=255)
                                                                                      cAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                     <  2:242502/150‑1 (MQ=255)
                                                                                      cAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                     <  2:410148/150‑1 (MQ=255)
                                                                                       aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                   <  2:976012/151‑1 (MQ=255)
                                                                                       aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                   <  1:788829/151‑1 (MQ=255)
                                                                                         tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                 >  1:325975/1‑151 (MQ=255)
                                                                                          aaaaCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                 <  2:886519/150‑1 (MQ=255)
                                                                                             aCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                             <  2:808573/151‑1 (MQ=255)
                                                                                              ccTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAcc                                            <  2:866452/151‑1 (MQ=255)
                                                                                                    ccTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGt                                       >  1:216366/1‑150 (MQ=255)
                                                                                                         gATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                 >  1:625290/1‑151 (MQ=255)
                                                                                                           tCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGt                                >  2:72966/1‑150 (MQ=255)
                                                                                                                 tACTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGa                          <  1:218015/150‑1 (MQ=255)
                                                                                                                   cTCGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATc                        >  2:211870/1‑150 (MQ=255)
                                                                                                                     cGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCa                       <  1:261540/149‑1 (MQ=255)
                                                                                                                     cGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc                      <  1:539366/150‑1 (MQ=255)
                                                                                                                      gTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCg                    <  1:677986/151‑1 (MQ=255)
                                                                                                                         aGCGATGCTTGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGat                  >  1:728804/1‑150 (MQ=255)
                                                                                                                                  tGCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGAt         <  1:237431/150‑1 (MQ=255)
                                                                                                                                   gCCGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa        <  2:630476/150‑1 (MQ=255)
                                                                                                                                    ccGGTGGCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGc      >  2:182193/1‑151 (MQ=255)
                                                                                                                                         ggCTAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCt  <  2:632964/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
CATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCT  >  NC_000913/3439928‑3440214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: