Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,822 T→C T70A (ACC→GCC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,8220TC100.0% 235.3 / NA 63T70A (ACC→GCC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/22);  total (41/22)

CGTCGTGGGTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCG  >  NC_000913/3440675‑3440968
                                                                                                                                                   |                                                                                                                                                  
cgtcgtGGGTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCt                                                                                                                                                 <  2:282956/151‑1 (MQ=255)
 gtcgtGGGTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTg                                                                                                                                                >  2:399057/1‑151 (MQ=255)
   cgtGGGTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGt                                                                                                                                               <  1:8867/150‑1 (MQ=255)
    gtGGGTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTAc                                                                                                                                             <  2:943541/151‑1 (MQ=255)
    gtGGGTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTAc                                                                                                                                             >  2:363339/1‑151 (MQ=255)
       ggTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCa                                                                                                                                          <  2:345220/151‑1 (MQ=255)
       ggTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCa                                                                                                                                          <  1:36006/151‑1 (MQ=255)
               cGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCGGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTac                                                                                                                                  >  2:488882/1‑151 (MQ=255)
                   tGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACAcc                                                                                                                               >  1:923695/1‑150 (MQ=255)
                    gcagtccaggggCCAAGGCCAGATTTATCCAAGGTAAGAATAACTCCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACAcc                                                                                                                               <  2:704000/140‑1 (MQ=255)
                    gCAGTCACGGGGCCAATGCCAGATTTATTCAAGGTAAGAATACCTTCATCTTTGCCCTGAACTCCCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCAt                                                                                                                             <  2:768909/151‑1 (MQ=255)
                    gCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCAt                                                                                                                             <  2:558194/151‑1 (MQ=255)
                         cacaGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAAtc                                                                                                                        >  1:232771/1‑151 (MQ=255)
                              ggCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAAc                                                                                                                   <  2:521254/151‑1 (MQ=255)
                              ggCCAATGCCAGATTAATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAAc                                                                                                                   <  1:713212/151‑1 (MQ=255)
                               gCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAAcc                                                                                                                  <  1:695377/151‑1 (MQ=255)
                                 cAATGCCAGATTTATTCAAGTTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTc                                                                                                                >  2:711966/1‑151 (MQ=255)
                                 cAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTc                                                                                                                >  1:786712/1‑151 (MQ=255)
                                      ccAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTc                                                                                                            >  2:607371/1‑150 (MQ=255)
                                      ccAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCa                                                                                                           <  2:752528/151‑1 (MQ=255)
                                       cAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCAc                                                                                                          >  1:423059/1‑151 (MQ=255)
                                       cAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCAc                                                                                                          >  1:862301/1‑151 (MQ=255)
                                          aTTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACcgc                                                                                                       >  2:959801/1‑151 (MQ=255)
                                           tttATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACcgc                                                                                                       <  2:433053/150‑1 (MQ=255)
                                             tATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCgcgc                                                                                                     >  2:244744/1‑150 (MQ=255)
                                              aTTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCa                                                                                                    >  1:632321/1‑150 (MQ=255)
                                                tCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAAcc                                                                                                 >  1:534826/1‑151 (MQ=255)
                                                  aaGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCg                                                                                               >  2:459991/1‑151 (MQ=255)
                                                    ggTAAGAATAACTCCATCTTTGCCCAGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGc                                                                                             <  2:557108/151‑1 (MQ=255)
                                                    cgTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGc                                                                                             >  2:990965/2‑151 (MQ=255)
                                                      tAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCAt                                                                                           >  2:25053/1‑151 (MQ=255)
                                                      tAAGAATAACTTCATCTTTGCCCGGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCAt                                                                                           >  1:918867/1‑151 (MQ=255)
                                                       aaGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCTGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATc                                                                                          <  1:743868/151‑1 (MQ=255)
                                                         gAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCg                                                                                         >  2:130798/1‑150 (MQ=255)
                                                            tAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATg                                                                                      >  2:222300/1‑150 (MQ=255)
                                                             aaCTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATga                                                                                     <  1:393639/150‑1 (MQ=255)
                                                                 tCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGc                                                                                 >  1:162864/1‑150 (MQ=255)
                                                                 tCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGc                                                                                 >  2:806625/1‑150 (MQ=255)
                                                                    tCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGa                                                                              >  1:276942/1‑150 (MQ=255)
                                                                    tCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGa                                                                              >  1:758255/1‑150 (MQ=255)
                                                                       ttGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATa                                                                           <  2:613558/150‑1 (MQ=255)
                                                                       ttGCCCTGAACTCCCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATa                                                                           <  1:228286/150‑1 (MQ=255)
                                                                       ttGCCCGGAACCCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGa                                                                              <  2:605696/147‑1 (MQ=255)
                                                                          cccTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGgc                                                                       >  1:537925/1‑151 (MQ=255)
                                                                          cccTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGgc                                                                       <  2:522232/151‑1 (MQ=255)
                                                                           ccTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTATTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGgcg                                                                      >  1:555058/1‑151 (MQ=255)
                                                                             tGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGgcgc                                                                     >  2:681711/1‑150 (MQ=255)
                                                                               aaCTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAg                                                                   >  1:67153/1‑150 (MQ=255)
                                                                                  tctcACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCg                                                               >  1:289100/1‑151 (MQ=255)
                                                                                        cGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTAccc                                                         >  1:268151/1‑151 (MQ=255)
                                                                                        cGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTAcc                                                          >  2:388529/1‑150 (MQ=255)
                                                                                                cTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATg                                                 >  2:155573/1‑151 (MQ=255)
                                                                                                 tttCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATgg                                                >  1:598706/1‑151 (MQ=255)
                                                                                                        ttGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGc                                         >  2:239108/1‑151 (MQ=255)
                                                                                                        ttGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGGATGGCCAAAGc                                         >  2:974916/1‑151 (MQ=255)
                                                                                                              aGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGct                                   >  2:98700/1‑151 (MQ=255)
                                                                                                                       aGGATATCTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCt                          >  1:828794/1‑151 (MQ=255)
                                                                                                                              cTTCCTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGt                   >  2:986095/1‑151 (MQ=255)
                                                                                                                                 ccTGAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                >  2:442660/1‑151 (MQ=255)
                                                                                                                                    gAACGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCtt             <  2:266970/151‑1 (MQ=255)
                                                                                                                                      aCGCCTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTgg           <  2:410901/151‑1 (MQ=255)
                                                                                                                                         ccTTCTTTGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgt        >  2:464896/1‑151 (MQ=255)
                                                                                                                                                tGGCGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCg  >  1:704821/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTCGTGGGTGATATCGGCTGCAGTCACAGGGCCAATGCCAGATTTATTCAAGGTAAGAATAACTTCATCTTTGCCCTGAACTCTCACCGCCAGCCCTTTCAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCG  >  NC_000913/3440675‑3440968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: