Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,462,301	A→G	T110A (ACG→GCG)	gspI →	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,462,301	0	A	G	100.0%	262.8 / NA	71	T110A (ACG→GCG)	gspI	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/38);  total (33/38)

AACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTT  >  NC_000913/3462163‑3462446                                                                                                                                           |                                                                                                                                                  aaCACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCaaa                                                                                                                                       <  2:162168/151‑1 (MQ=255)      gTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATc                                                                                                                                  <  1:228679/151‑1 (MQ=255)           gTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataa                                                                                                                             <  2:862128/151‑1 (MQ=255)             tCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataat                                                                                                                            >  2:457365/1‑150 (MQ=255)             tCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGataat                                                                                                                            <  2:581990/150‑1 (MQ=255)              cTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACGGGCGCAGCGATATCCACTCATTCAAAGACGGCACGCTTCTGGAACGCACCATAAAGGTGACATTACCCAGCGGTCGGACAACCTCGCGTGCGCGTTATCAAAGCATCGataata                                                                                                                           >  2:32387/1‑150 (MQ=255)               tGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCGGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGAtaataa                                                                                                                          >  2:328666/1‑150 (MQ=255)               tGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGAtaataa                                                                                                                          >  2:627961/1‑150 (MQ=255)                ggCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATaaa                                                                                                                         <  2:264753/150‑1 (MQ=255)                ggCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATaaa                                                                                                                         <  1:808239/150‑1 (MQ=255)                  cAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATcc                                                                                                                      >  1:488174/1‑151 (MQ=255)                  cAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATcc                                                                                                                      >  2:550529/1‑151 (MQ=255)                     agagTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCggg                                                                                                                   <  1:788778/151‑1 (MQ=255)                      gagTTAATAAATGGCGAATAGATGATCTGGCGATGCGATATCCACTCAAGCAAAGACGTCACCCTTCTGTGACGCTCCATAACGGTGACATTACCCAGCGGTCAGACAGCCTCGCTTGCGCGTTATCAAAGCCTCGATAATAAATCCg                                                                                                                     <  2:401627/148‑1 (MQ=255)                      gagTTAATAAATGGCGAAGAGTGGAACTGGTGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggc                                                                                                                  <  1:578230/151‑1 (MQ=255)                      gagTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggc                                                                                                                  <  1:724101/151‑1 (MQ=255)                       agTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggca                                                                                                                 <  1:893654/151‑1 (MQ=255)                       agTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggc                                                                                                                  >  2:838791/1‑150 (MQ=255)                        gTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGggc                                                                                                                  >  1:15194/1‑149 (MQ=255)                              aaaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGcaca                                                                                                           >  2:513017/1‑150 (MQ=255)                               aaTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAg                                                                                                         <  2:647726/151‑1 (MQ=255)                                aTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAga                                                                                                        <  1:538144/151‑1 (MQ=255)                                aTGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAga                                                                                                        <  1:957113/151‑1 (MQ=255)                                      aaGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTa                                                                                                  <  2:243483/151‑1 (MQ=255)                                       agagTGGAACTGGCGCAGCTATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTaa                                                                                                 <  2:179498/151‑1 (MQ=255)                                       agagTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTaa                                                                                                 <  2:365940/151‑1 (MQ=255)                                          gTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCg                                                                                              >  1:490080/1‑151 (MQ=255)                                               aCTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcagc                                                                                         >  2:583874/1‑151 (MQ=255)                                                 tGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCcagc                                                                                         >  2:935732/1‑149 (MQ=255)                                                     gcAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGtt                                                                                   >  1:617513/1‑151 (MQ=255)                                                      cAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGttt                                                                                  >  1:798929/1‑151 (MQ=255)                                                           atatCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACAt                                                                             <  1:606925/151‑1 (MQ=255)                                                                cACTCAAGCAAAGCCGGCACGCTTCTTGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCTCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCTCATTGCTg                                                                        <  2:304270/151‑1 (MQ=255)                                                                cACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTg                                                                        >  1:825/1‑151 (MQ=255)                                                                cACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTg                                                                        >  2:646226/1‑151 (MQ=255)                                                                      aGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTc                                                                  <  2:809004/151‑1 (MQ=255)                                                                           aGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATggc                                                             <  1:544905/151‑1 (MQ=255)                                                                                    gCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGc                                                    >  1:174550/1‑151 (MQ=255)                                                                                     cTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCg                                                   >  1:869884/1‑151 (MQ=255)                                                                                      ttCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCg                                                   >  1:732340/1‑150 (MQ=255)                                                                                          ggAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGAtttt                                              <  1:50913/151‑1 (MQ=255)                                                                                          ggAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGAtttt                                              >  1:975149/1‑151 (MQ=255)                                                                                          ggAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGAtt                                                <  1:15755/149‑1 (MQ=255)                                                                                             aCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtctc                                           <  2:522647/151‑1 (MQ=255)                                                                                             aCGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtctc                                           <  2:485484/151‑1 (MQ=255)                                                                                              cGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTtctc                                           >  1:673778/1‑150 (MQ=255)                                                                                                cACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAAt                                        <  2:148678/151‑1 (MQ=255)                                                                                                 aCCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATg                                       >  2:26388/1‑151 (MQ=255)                                                                                                 aCCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATg                                       <  1:975617/151‑1 (MQ=255)                                                                                                  ccATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATg                                       <  2:466694/150‑1 (MQ=255)                                                                                                      aaCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAg                                  <  2:26316/151‑1 (MQ=255)                                                                                                       aCGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAg                                  >  2:716384/1‑150 (MQ=255)                                                                                                        cGGTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCg                                >  1:381494/1‑151 (MQ=255)                                                                                                         ggTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGt                               <  1:567634/151‑1 (MQ=255)                                                                                                         ggTGACATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGt                               <  2:614168/151‑1 (MQ=255)                                                                                                             aCATTACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTg                           <  1:427720/151‑1 (MQ=255)                                                                                                                ttACCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCg                        >  1:821545/1‑151 (MQ=255)                                                                                                                  aCCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                      >  2:147082/1‑151 (MQ=255)                                                                                                                  aCCCAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                      >  2:227854/1‑151 (MQ=255)                                                                                                                   cccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGtt                      <  1:298555/150‑1 (MQ=255)                                                                                                                    ccAGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGttt                     >  1:821902/1‑150 (MQ=255)                                                                                                                      aGCGGTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATg                  >  2:566256/1‑151 (MQ=255)                                                                                                                       gCGGTCAGACAGCCTCGCTTGCGAGTTATCAAAGCCTCGATAATAAATCCGGGCAGGCACAAGATGATTAAGCGCCAGCAGGGTTTCACACTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTTCTGGCGTTTa                    <  2:848612/148‑1 (MQ=255)                                                                                                                         ggTCAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtt               >  1:897920/1‑151 (MQ=255)                                                                                                                            cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAttttt            <  2:289753/151‑1 (MQ=255)                                                                                                                            cAGACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGAtttt             <  2:558293/150‑1 (MQ=255)                                                                                                                              gACAACCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTc          >  1:89062/1‑151 (MQ=255)                                                                                                                                 aaCCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCa       <  2:227090/151‑1 (MQ=255)                                                                                                                                 aaCCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCa       <  2:49742/151‑1 (MQ=255)                                                                                                                                  aCCTCGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCg         >  2:65398/1‑148 (MQ=255)                                                                                                                                      cGCTTGCGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCtt  >  1:465069/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                  AACACGTCCAGTTCTGGCAAAGAGTTAATAAATGGCGAAGAGTGGAACTGGCGCAGCGATATCCACTCAAGCAAAGACGGCACGCTTCTGGAACGCACCATAACGGTGACATTACCCAGCGGTCAGACAACCTCGCTTACGCGTTATCAAAGCATCGATAATAAATCCGGGCAGGCACAAGATGATTAATCGCCAGCAGGGTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTT  >  NC_000913/3462163‑3462446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑