Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,486,190 A→G Y24C (TAC→TGC)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,1900AG100.0% 285.8 / NA 73Y24C (TAC→TGC) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/38);  total (35/38)

AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGG  >  NC_000913/3486045‑3486334
                                                                                                                                                 |                                                                                                                                                
aaGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCAt                                                                                                                                             >  1:283061/1‑151 (MQ=255)
 aGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATc                                                                                                                                            <  1:242583/151‑1 (MQ=255)
    atatTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCa                                                                                                                                          >  2:319353/1‑150 (MQ=255)
    atatTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCa                                                                                                                                          >  1:304391/1‑150 (MQ=255)
     tatTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCa                                                                                                                                          >  1:635775/1‑149 (MQ=255)
      atTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCa                                                                                                                                          >  1:147306/1‑148 (MQ=255)
          cGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCa                                                                                                                                    <  1:247110/150‑1 (MQ=255)
            gCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGc                                                                                                                                 >  1:572995/1‑151 (MQ=255)
              aaTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCtt                                                                                                                               <  1:232000/151‑1 (MQ=255)
                 ccAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTAtt                                                                                                                            >  1:135134/1‑151 (MQ=255)
                     agaCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCAc                                                                                                                         <  2:595788/150‑1 (MQ=255)
                      gaCAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCa                                                                                                                       <  1:325820/151‑1 (MQ=255)
                         aGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAggg                                                                                                                    <  1:87816/151‑1 (MQ=255)
                          gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGt                                                                                                                   <  2:15776/151‑1 (MQ=255)
                            ggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGt                                                                                                                   <  2:790861/149‑1 (MQ=255)
                            ggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTg                                                                                                                  <  1:176148/150‑1 (MQ=255)
                              cgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaa                                                                                                                >  1:774227/1‑150 (MQ=255)
                               gTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaa                                                                                                               <  2:53494/150‑1 (MQ=255)
                                ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                              <  1:349445/150‑1 (MQ=255)
                                ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                              <  2:431083/150‑1 (MQ=255)
                                 tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaaa                                                                                                             <  2:100325/150‑1 (MQ=255)
                                 tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                              >  1:844233/1‑149 (MQ=255)
                                 tATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaa                                                                                                              >  1:567632/1‑149 (MQ=255)
                                      ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                       <  1:866947/151‑1 (MQ=255)
                                        ctctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAAc                                                                                                     >  1:138109/1‑151 (MQ=255)
                                           tGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGAGAAAAAGCGGAAACGc                                                                                                   >  1:593191/1‑150 (MQ=255)
                                                  aGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGtactac                                                                                           >  2:361374/1‑151 (MQ=255)
                                                   gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACa                                                                                          <  1:764690/151‑1 (MQ=255)
                                                      tataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCg                                                                                       >  2:126536/1‑151 (MQ=255)
                                                        taACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                     >  2:529219/1‑151 (MQ=255)
                                                         aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                     >  2:11255/1‑150 (MQ=255)
                                                           cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaaa                                                                                  <  1:444601/151‑1 (MQ=255)
                                                           cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTaa                                                                                   >  1:444875/1‑150 (MQ=255)
                                                           cAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTa                                                                                    >  2:660464/1‑149 (MQ=255)
                                                                   aaCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt                                                                          >  2:799066/1‑151 (MQ=255)
                                                                     ccGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTgg                                                                        >  1:983866/1‑151 (MQ=255)
                                                                      cgcgCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc                                                                       >  2:403207/1‑151 (MQ=255)
                                                                        cgcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGc                                                                       >  1:728283/1‑149 (MQ=255)
                                                                         gcATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGt                                                                    >  1:232854/1‑151 (MQ=255)
                                                                           aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGc                                                                  >  2:239708/1‑151 (MQ=255)
                                                                           aTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGc                                                                  <  1:593956/151‑1 (MQ=255)
                                                                                   tGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCa                                                            <  2:275518/149‑1 (MQ=255)
                                                                                    ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa                                                          <  2:49665/150‑1 (MQ=255)
                                                                                    ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa                                                          <  1:283164/150‑1 (MQ=255)
                                                                                       aaaCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACg                                                      <  1:958086/151‑1 (MQ=255)
                                                                                          ccGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa                                                     >  1:526617/1‑149 (MQ=255)
                                                                                           cGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAag                                                   >  1:424859/1‑150 (MQ=255)
                                                                                             cAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAagag                                                 <  1:289444/150‑1 (MQ=255)
                                                                                              aaaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAaga                                                  <  2:246382/148‑1 (MQ=255)
                                                                                               aaCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACTAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGt                                              <  2:145176/151‑1 (MQ=255)
                                                                                                   gtcccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaaa                                           <  1:906762/148‑1 (MQ=255)
                                                                                                     cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGa                                         <  1:580734/150‑1 (MQ=255)
                                                                                                        gACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATg                                     <  2:467473/151‑1 (MQ=255)
                                                                                                        gACTCTCGAATGGTTCTTGTCTCATCGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGaaa                                       >  1:710752/1‑149 (MQ=255)
                                                                                                         aCTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCGCCAGGGTGAAAAAGCGGAAACGCTGTAGGACAGCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGa                                    <  2:650919/151‑1 (MQ=255)
                                                                                                           tctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                  >  1:515942/1‑151 (MQ=255)
                                                                                                           tctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                  >  1:916069/1‑151 (MQ=255)
                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATcc                                 <  1:508018/149‑1 (MQ=255)
                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                  <  2:526562/148‑1 (MQ=255)
                                                                                                              cGAATGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCct                                <  1:38699/150‑1 (MQ=255)
                                                                                                                aaTGGTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAGATGATCCtctc                             >  2:827136/1‑151 (MQ=255)
                                                                                                                    gTTCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTAt                         <  2:631526/151‑1 (MQ=255)
                                                                                                                      tCTTGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATc                        <  2:547625/150‑1 (MQ=255)
                                                                                                                       catGTCTCATCGCGACATTCATAAGTGCCCACCCAAGAGCACGCTTATTCGCCGGGGTGAAAAAGCGGAAACGCTGTACTACATCATTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGACGAGGGTAAAGAAATGATCGTTTCCTATcag                      <  2:303000/149‑3 (MQ=255)
                                                                                                                         tGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCt                       <  2:200210/148‑1 (MQ=255)
                                                                                                                         tGTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGa                     >  1:81593/1‑150 (MQ=255)
                                                                                                                          gTCTCATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAAt                   <  2:283027/151‑1 (MQ=255)
                                                                                                                           tctcATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATc                  >  2:151961/1‑151 (MQ=255)
                                                                                                                              cATTGCCACATTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAgg               >  2:384449/1‑151 (MQ=255)
                                                                                                                                     acaTTCATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAttt        <  2:567573/151‑1 (MQ=255)
                                                                                                                                          cATAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt    <  2:156761/150‑1 (MQ=255)
                                                                                                                                           aTAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTTAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTgg  >  1:502835/1‑151 (MQ=255)
                                                                                                                                           aTAAGTGCCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTgg  >  1:368698/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGG  >  NC_000913/3486045‑3486334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: