Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,505,884	A→G	L46P (CTG→CCG)	yhfS ←	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,505,884	0	A	G	100.0%	201.3 / NA	62	L46P (CTG→CCG)	yhfS	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/37);  total (25/37)

AGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTC  >  NC_000913/3505743‑3506030                                                                                                                                              |                                                                                                                                                    agaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCg                                                                                                                                            >  2:745007/1‑151 (MQ=255)   aCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCg                                                                                                                                            <  2:463750/149‑1 (MQ=255)     gCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTa                                                                                                                                         >  2:690256/1‑150 (MQ=255)        gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAg                                                                                                                                       <  1:851006/149‑1 (MQ=255)         ccccGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCt                                                                                                                                    >  2:323261/1‑151 (MQ=255)           ccGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTaa                                                                                                                                  <  2:385600/151‑1 (MQ=255)                 tGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CAc                                                                                                                            <  1:57991/150‑1 (MQ=255)                    gCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CTCCGc                                                                                                                         <  1:430011/150‑1 (MQ=255)                     cAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAACCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGcc                                                                                                                        >  2:197456/1‑150 (MQ=255)                      aaaGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAg                                                                                                                      <  1:864568/151‑1 (MQ=255)                         gccgccAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTa                                                                                                                    >  1:811742/1‑150 (MQ=255)                          cccccACCCGGGCGCAAACCGCCCCAGCCCCCGCCCCCTCCCCCAGCGCCGCAGCCTGGGGGTGAAAGCCATCAGCCAGCCCCTGTCCCACACGCGGGGTACCGCGGGGTTGATTCGGTCCTGGCGTAAAGCCTAAAT‑CACCGCCAGt                                                                                                                     <  2:492889/145‑1 (MQ=255)                                 cccGGCGCGAATCGCGCCAGTCCCCGCCCCCTGCCCCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCCCCTGTCCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTc                                                                                                            <  1:603207/150‑1 (MQ=255)                                           aTCGCCCCATTCCCCGCGCCCTGCCCCAGCGCCGCAGCTTGTGCGTGAAATGCATCAGCCAGCCCCTGTTCCACACGCTGGTTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAC‑CACCGCCAGTAAGAAACTCGCTGCCggg                                                                                                   <  1:745999/149‑1 (MQ=255)                                              gcgcCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAAt                                                                                              >  1:861326/1‑151 (MQ=255)                                                   aGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCg                                                                                          >  2:553340/1‑150 (MQ=255)                                                   aGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCg                                                                                          >  1:879426/1‑150 (MQ=255)                                                    gTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGAc                                                                                        >  2:755660/1‑151 (MQ=255)                                                      ccccGGGCCCGACACCAGCGCCACAGCCGGTGGGGAAAATGCTTCAGCCAGCCCTGGTCCCCCACGCTGTAAAACGCGGGTTTGATGCGGTCCGGGGTTTAAGCTTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCg                                                                                          <  2:871911/147‑1 (MQ=255)                                                       cccgcccccgccCCCAGCGCCGCAGCCTGTGCGTAAAATGCATCAGCCAGCCCCTGTCCCACACGCTGGGTAACGCGGGGTTGATTGGGTCCTGGCGTTAAGCAAAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACaaa                                                                                     <  2:832525/151‑1 (MQ=255)                                                             cccTGCACCAGCGCCGCAGCCTGTGGGTAAAATGCATCAGCCAGCCCCTGTCCCACACGCGGGTTAACGCGCGGTTGATTCGGTCCGGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATg                                                                                   <  1:404927/147‑1 (MQ=255)                                                                   aCCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATccacc                                                                          >  2:343657/1‑150 (MQ=255)                                                                    ccggcgccgcgccCTGTGCGAGAAACCCAACAGCAAGCCCTTGTCCCACACGCGGGGTAACGCGGGGTTGATTCGTTCCGGGCGTAAGCCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATccac                                                                           <  1:241473/145‑1 (MQ=255)                                                                    ccAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCGGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATccacca                                                                         <  1:866996/150‑1 (MQ=255)                                                                    ccAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTCCCACCCGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATccacca                                                                         <  2:41686/150‑1 (MQ=255)                                                                      aGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCGGGGTAACGCGCGGTTGATTCGGTCCTGGCTTTAACCCAAAAA‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATct                                                                             <  1:762729/144‑2 (MQ=255)                                                                         gccgcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCa                                                                    >  1:775843/1‑150 (MQ=255)                                                                          ccgcAGCCTGTGCTTAAAATGCATCAGCCACCCCCTGTCCCACCCGCTGGGTACCGCGCGGTTGATTCGGTCCTGGCTTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAgcg                                                                      <  1:68376/147‑1 (MQ=255)                                                                            gcggcgtgtgCGTGAAATGCATCAGCCACCCCTTGTCCCACCCGCTGGGTAACGGGGGGTTGATTCGGTCCTGGCGTTAACCTTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCa                                                                    <  2:684043/141‑1 (MQ=255)                                                                              aGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGc                                                              >  2:221738/1‑151 (MQ=255)                                                                              aGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGc                                                              >  2:431235/1‑151 (MQ=255)                                                                                 cTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCttt                                                           >  1:562753/1‑151 (MQ=255)                                                                                   gtgCGTGAAATGCATCAGCCAGCCCCTGTTCCACACGCGGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtg                                                         <  1:666698/151‑1 (MQ=255)                                                                                   gtgCGTGAAATGCATCACCCACCCCTTGTTCCACCCGCGGGGTACCGCGGGGTTGTTTCGGTCCTGGCGTTAACCCAAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCttt                                                           <  1:313065/149‑1 (MQ=255)                                                                                     gggTGAAATGCATCAGCCAGCACCTGTTCCACACGCGGGGAAACGCGCGGTTGATTCGGTCCTGGCGTTAGCCCTAAAC‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCtttt                                                          <  2:715214/146‑1 (MQ=255)                                                                                     gCGTGAAATGCATCAGCCAGCCCCTGTCCCACACGCGGGGTAACGCGCGGTTGATTCGGTCCTGGCGTAAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgc                                                        <  1:386261/150‑1 (MQ=255)                                                                                      cGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctg                                                      >  2:998316/1‑151 (MQ=255)                                                                                          aaaTGCATCAGCCAGCACCTTTTCCACCCGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTgcgc                                                   <  1:44738/150‑1 (MQ=255)                                                                                           aaggCATCAGCCAGCACCTGTCCCACACGCGGGGTACCGCGCGGTTGATGCGGTCCGGGCGTTAAGCCAAAAT‑CCCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCt                                                 <  2:754389/148‑1 (MQ=255)                                                                                             tGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTc                                                >  2:720861/1‑150 (MQ=255)                                                                                              gCATCCACCAGCACCTGTTCCACACGCTGGGTAACGCACGGTTGATTCGGTCCTAGCGTTCAGCCTAAAT‑CACCTCCAGTACGAAACTCGCTGCCGGTGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCTcc                                                  >  2:292964/1‑147 (MQ=255)                                                                                                 tCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCaata                                            >  1:241045/1‑150 (MQ=255)                                                                                                          aCCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGc                                  >  2:478140/1‑151 (MQ=255)                                                                                                            cTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCtt                                >  2:714616/1‑151 (MQ=255)                                                                                                            cTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCtt                                >  1:335604/1‑151 (MQ=255)                                                                                                               ttCCACACGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCTTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCttt                               <  1:403588/149‑1 (MQ=255)                                                                                                               tcccACCCGCTGGGTAAGGGGGGGTTGATTGGGTCCGGGCGTTAAGCCAAAAC‑CCCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCtt                                <  2:582942/146‑1 (MQ=255)                                                                                                               tcccACCCGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTg                              <  1:228832/148‑1 (MQ=255)                                                                                                                 ccACCCGGTGGTTACCGCGCGGTTGTTTCGGTCCTGGCTTAAACCCAAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTg                              <  2:70826/148‑1 (MQ=255)                                                                                                                 ccACACGCTGGGTAACGCGCGGTTGTTTCGGTCCTGGCGTAAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGc                             <  2:700584/149‑1 (MQ=255)                                                                                                                    cccgcgGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCAAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAgg                        <  2:872955/149‑1 (MQ=255)                                                                                                                    cacGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAgg                        >  2:645657/1‑151 (MQ=255)                                                                                                                     acGCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCATCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAgg                        >  1:386780/1‑150 (MQ=255)                                                                                                                       gCTGGGTAACGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGa                       <  1:186183/149‑1 (MQ=255)                                                                                                                          gggTACCGCGCGGTTGATTCGTTCCTGGCGTTAGCCCTAAAT‑CCCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGaa                      <  2:811660/147‑1 (MQ=255)                                                                                                                          gggTACCGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCATCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACg                   <  2:386735/150‑1 (MQ=255)                                                                                                                               aCGCGCGGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTc              >  1:804456/1‑150 (MQ=255)                                                                                                                                cgcgcgGTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCa             <  1:137152/150‑1 (MQ=255)                                                                                                                                  cgcgGTTGATCCGGTCCGGGCGTAAAGCCAAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATa           <  2:877991/150‑1 (MQ=255)                                                                                                                                     ggTTGATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAg          <  1:291229/148‑1 (MQ=255)                                                                                                                                         gATTCGGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGcctt   >  2:172816/1‑151 (MQ=255)                                                                                                                                          aTTCGGCCCTGGCGTT‑AGCCTTAATCCGCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGccttc  <  2:815191/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    AGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAAT‑CACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTC  >  NC_000913/3505743‑3506030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑