Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,588,203 A→G H326H (CAT→CAC)  ugpC ← sn‑glycerol‑3‑phosphate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,588,2030AG100.0% 180.0 / NA 49H326H (CAT→CAC) ugpCsn‑glycerol‑3‑phosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/25);  total (24/25)

GGGCCAGCTTACCGCCGCCACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCG  >  NC_000913/3588056‑3588347
                                                                                                                                                   |                                                                                                                                                
gggCCAGCTTACCGCCGCCACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTgc                                                                                                                                               >  1:182917/1‑151 (MQ=255)
 ggCCAGCTTACCGCCGCCACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTgcg                                                                                                                                              <  1:432405/151‑1 (MQ=255)
  gCCAGCTTACCGCCGCCACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTgcgc                                                                                                                                             <  1:645837/151‑1 (MQ=255)
     aGCTTACCGCCGCCACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGcc                                                                                                                                            >  2:346488/1‑149 (MQ=255)
           ccgccgCCACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcac                                                                                                                                    >  1:475588/1‑151 (MQ=255)
                gccACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCaccacc                                                                                                                                >  1:858968/1‑150 (MQ=255)
                gccACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCAccacca                                                                                                                               <  1:605889/151‑1 (MQ=255)
                      aTGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCt                                                                                                                         <  2:678388/151‑1 (MQ=255)
                           cgacgaTGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCg                                                                                                                    <  1:484687/151‑1 (MQ=255)
                               gaTGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGcccc                                                                                                                <  1:378634/151‑1 (MQ=255)
                               gaTGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGcccc                                                                                                                <  1:93024/151‑1 (MQ=255)
                                aTGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCa                                                                                                               <  1:619404/151‑1 (MQ=255)
                                   cGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCg                                                                                                            >  2:938458/1‑151 (MQ=255)
                                   cGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCg                                                                                                            >  2:647063/1‑151 (MQ=255)
                                             gCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGcc                                                                                                  >  1:771998/1‑151 (MQ=255)
                                              ccAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCa                                                                                                 <  1:1001527/151‑1 (MQ=255)
                                                    aCTCATACTCTTTGTCCTGTCTCACCAACAAAAAGATGCAGCTGATTTTCCGGCAGATGCAGCGACAGCGTGCTGCCTGCCGTCGTGCGCACCGCGTGCGCGAGGCGCACCATCGGCGTCTGCTCGCCCCAGCGTCCGTGAGCCAg                                                                                                <  2:601808/146‑1 (MQ=255)
                                                        aTACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgc                                                                                       >  1:885643/1‑151 (MQ=255)
                                                         tACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgcg                                                                                      >  2:72338/1‑151 (MQ=255)
                                                          aCTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgcgc                                                                                     <  1:826179/151‑1 (MQ=255)
                                                          aCTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgcg                                                                                      <  1:578674/150‑1 (MQ=255)
                                                             cGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGa                                                                                  <  2:643988/151‑1 (MQ=255)
                                                                tGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAgg                                                                                <  2:27860/150‑1 (MQ=255)
                                                                tGTCCTGTTTAACCATCAAAAAGATTCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCCGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAgg                                                                                >  2:134058/1‑150 (MQ=255)
                                                                 gTCCTGTTTCACCACCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCGGCCGTCGGGCGCACCTGGGGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTGCGTGCGCCAGGTTATCTGCGCCGAg                                                                                 <  2:736156/148‑1 (MQ=255)
                                                                        ttCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAg                                                                        >  1:418840/1‑150 (MQ=255)
                                                                           aCCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGAAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCgtg                                                                    >  2:4367/1‑151 (MQ=255)
                                                                              aTCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTcc                                                                 >  1:751388/1‑151 (MQ=255)
                                                                                       aTGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATc                                                        >  2:738154/1‑151 (MQ=255)
                                                                                        tGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCg                                                       >  1:469664/1‑151 (MQ=255)
                                                                                               gATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTAcgcc                                                >  2:285744/1‑151 (MQ=255)
                                                                                                 ttttCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCCGCGTGCCCATCACCATCGGTAcgcc                                                >  2:154230/1‑149 (MQ=255)
                                                                                                    tCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCtt                                           >  2:737827/1‑151 (MQ=255)
                                                                                                    tCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCt                                            <  1:559190/150‑1 (MQ=255)
                                                                                                     ccGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTc                                          <  2:340491/151‑1 (MQ=255)
                                                                                                      cGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCATCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTcg                                         <  2:75323/151‑2 (MQ=255)
                                                                                                       gCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTctg                                        >  1:383830/1‑151 (MQ=255)
                                                                                                        ccAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTctgc                                       >  2:558553/1‑151 (MQ=255)
                                                                                                           gATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTctgcct                                     <  2:924830/150‑1 (MQ=255)
                                                                                                              gCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCg                                  <  2:429927/150‑1 (MQ=255)
                                                                                                                 gCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCt                              <  1:343429/151‑1 (MQ=255)
                                                                                                                  ccACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCtt                             <  2:166883/151‑1 (MQ=255)
                                                                                                                     caGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAgc                          >  1:804715/1‑151 (MQ=255)
                                                                                                                           gctgcCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAat                     <  2:926749/150‑1 (MQ=255)
                                                                                                                              gcctgccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGt                 >  2:2107/1‑151 (MQ=255)
                                                                                                                                   ccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCgg            <  2:45508/151‑1 (MQ=255)
                                                                                                                                     gTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGc          >  1:630155/1‑151 (MQ=255)
                                                                                                                                       cGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCg         <  2:622184/150‑1 (MQ=255)
                                                                                                                                               ccTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCg  >  1:383212/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGGCCAGCTTACCGCCGCCACGATGAGCGACGATGCGGGGATAAGGCCAGTTACTCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGGTACGCCGCCTTCTGCCTGCGAGCTTAGCGCAATATGTTCCGGGCGAATGCCG  >  NC_000913/3588056‑3588347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑