Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,606,883	G→A	E145K (GAG→AAG)	zntA →	zinc, cobalt and lead efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,606,883	0	G	A	100.0%	262.4 / NA	68	E145K (GAG→AAG)	zntA	zinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/38);  total (30/38)

ATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTG  >  NC_000913/3606736‑3607030                                                                                                                                                    |                                                                                                                                                    aTTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGc                                                                                                                                                  >  1:855531/1‑151 (MQ=255)    cGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGTTTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAg                                                                                                                                                <  2:72908/150‑1 (MQ=255)    cGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAg                                                                                                                                                <  1:465150/150‑1 (MQ=255)        cacaAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCa                                                                                                                                            >  1:196730/1‑150 (MQ=255)           aaGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGttcattca                                                                                                                                        <  2:746642/151‑1 (MQ=255)               tGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATcc                                                                                                                                     <  1:95660/150‑1 (MQ=255)               tGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATcc                                                                                                                                     <  2:770145/150‑1 (MQ=255)                gAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCg                                                                                                                                    <  2:450175/150‑1 (MQ=255)                 aaTCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGtt                                                                                                                                  <  1:356646/151‑1 (MQ=255)                 aaTCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGtt                                                                                                                                  <  1:365298/151‑1 (MQ=255)                      gcgcTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCgg                                                                                                                               <  2:182923/149‑1 (MQ=255)                      gcgcTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCgg                                                                                                                               <  2:265945/149‑1 (MQ=255)                      gcgcTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGc                                                                                                                             >  1:925708/1‑151 (MQ=255)                       cgcTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGc                                                                                                                             >  2:466984/1‑150 (MQ=255)                           gCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTg                                                                                                                        >  1:402873/1‑151 (MQ=255)                                 aGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGttt                                                                                                                  <  1:995472/151‑1 (MQ=255)                                  gCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGttt                                                                                                                  >  2:402269/1‑150 (MQ=255)                                  gCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTa                                                                                                                 <  2:855256/151‑1 (MQ=255)                                   cAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTAt                                                                                                                <  2:330765/151‑1 (MQ=255)                                        tATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATcgcg                                                                                                            >  2:287225/1‑150 (MQ=255)                                           tCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGAcc                                                                                                         >  1:435875/1‑150 (MQ=255)                                            cccTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCAc                                                                                                       <  1:241756/151‑1 (MQ=255)                                                  gcgATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGt                                                                                                 >  1:12926/1‑151 (MQ=255)                                                   cgATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAGAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGtt                                                                                                >  2:874035/1‑151 (MQ=255)                                                     aTGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTgg                                                                                              >  2:1917/1‑151 (MQ=255)                                                      tGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTggg                                                                                             >  2:896734/1‑151 (MQ=255)                                                                gccgAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGa                                                                                   <  2:362646/151‑1 (MQ=255)                                                                      gaaCCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGGGCCCTCGTTGGTTGGGCTGTACCCGACTGCTc                                                                             <  2:86363/151‑1 (MQ=255)                                                                         ccGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTc                                                                          >  2:73082/1‑151 (MQ=255)                                                                             aaGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGc                                                                      >  2:639127/1‑151 (MQ=255)                                                                                cATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCAtt                                                                   <  2:875407/151‑1 (MQ=255)                                                                                cATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCAtt                                                                   <  1:427669/151‑1 (MQ=255)                                                                                  tCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTAc                                                                 >  2:548030/1‑151 (MQ=255)                                                                                    aCGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACgg                                                               <  1:904790/151‑1 (MQ=255)                                                                                          gAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGAt                                                          <  2:333080/150‑1 (MQ=255)                                                                                           aaaGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATc                                                         <  2:618574/150‑1 (MQ=255)                                                                                            aaGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCaa                                                       <  1:199574/151‑1 (MQ=255)                                                                                            aaGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCa                                                        >  2:611672/1‑150 (MQ=255)                                                                                            aaGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCa                                                        >  1:711883/1‑150 (MQ=255)                                                                                             agagAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCaaa                                                      <  1:629506/151‑1 (MQ=255)                                                                                               agaATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATc                                                    <  1:189936/151‑1 (MQ=255)                                                                                               agaATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGATGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAAt                                                     >  1:51146/1‑150 (MQ=255)                                                                                                gaATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATc                                                    >  2:760192/1‑150 (MQ=255)                                                                                                 aaTCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATcc                                                   <  1:820523/150‑1 (MQ=255)                                                                                                      gCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCgg                                                 <  1:398821/147‑1 (MQ=255)                                                                                                      gCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAg                                              <  2:569935/150‑1 (MQ=255)                                                                                                       ccGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCt                                            <  2:429993/151‑1 (MQ=255)                                                                                                         gCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTAc                                          >  1:50612/1‑151 (MQ=255)                                                                                                                aCGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGcc                                    <  2:587149/150‑1 (MQ=255)                                                                                                                  gCTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCAtt                                 <  2:225412/151‑1 (MQ=255)                                                                                                                   cTAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTg                                >  2:522566/1‑151 (MQ=255)                                                                                                                    tAATCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCAtt                                 >  1:24948/1‑149 (MQ=255)                                                                                                                      aTCGTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTAATGGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGaaa                             <  2:431225/151‑1 (MQ=255)                                                                                                                         gTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCt                          >  2:881542/1‑151 (MQ=255)                                                                                                                         gTGATGATGGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCt                          >  1:147682/1‑151 (MQ=255)                                                                                                                              gatgGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAAt                      <  2:196803/150‑1 (MQ=255)                                                                                                                               atgGCAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGa                    <  1:317078/151‑1 (MQ=255)                                                                                                                                   cAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGt                >  1:271292/1‑151 (MQ=255)                                                                                                                                   cAATCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGt                >  1:179669/1‑151 (MQ=255)                                                                                                                                    aaTCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGt                >  1:230090/1‑150 (MQ=255)                                                                                                                                    aaTCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTa               <  2:196707/151‑1 (MQ=255)                                                                                                                                    aaTCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTa               <  1:766608/151‑1 (MQ=255)                                                                                                                                     aTCAGCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCAGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAg              <  1:803361/151‑1 (MQ=255)                                                                                                                                         gCTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCg           <  2:259860/150‑1 (MQ=255)                                                                                                                                          cTGGGGTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCt         >  1:227090/1‑151 (MQ=255)                                                                                                                                            ggggTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTAt       >  2:595529/1‑151 (MQ=255)                                                                                                                                             gggTCTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTAtt      <  2:993707/151‑1 (MQ=255)                                                                                                                                                 cTGAAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTg  >  2:252209/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATTCGTGCACAAGTTGAATCTGCGCTGCAAAAAGCAGGCTATTCCCTGCGCGATGAACAGGCCGCCGAAGAACCGCAAGCATCACGCCTGAAAGAGAATCTGCCGCTGATTACGCTAATCGTGATGATGGCAATCAGCTGGGGTCTGGAGCAGTTCAATCATCCGTTCGGGCAACTGGCGTTTATCGCGACCACGCTGGTTGGGCTGTACCCGATTGCTCGTCAGGCATTACGGTTGATCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTG  >  NC_000913/3606736‑3607030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑