Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	43.8%	93.1 / 43.9	73	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (35/6);  new base A (0/32);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 9.37e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645                                                                                                                                                            |                                                                                                                                                      tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAatat                                                                                                                                                       >  1:938421/1‑151 (MQ=255)   tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAAtata                                                                                                                                                      >  1:861585/1‑150 (MQ=255)    aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATaa                                                                                                                                                     <  2:1035650/150‑1 (MQ=255)     aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATaaa                                                                                                                                                    >  2:1000511/1‑150 (MQ=255)     aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATaaa                                                                                                                                                    >  2:793427/1‑150 (MQ=255)           aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATccc                                                                                                                                             >  2:79680/1‑151 (MQ=255)            ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                            >  2:69909/1‑151 (MQ=255)                gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                                        >  1:824979/1‑151 (MQ=255)                gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                                        >  1:856704/1‑151 (MQ=255)                  ccATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                                       >  1:970800/1‑150 (MQ=255)                     tttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                                   >  2:592130/1‑151 (MQ=255)                        ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                                 >  1:40366/1‑150 (MQ=255)                            cttttttttCTGCTCGTTTTTATTACACGCATTCTTCTTGCTGCGCAAGCGTTTCCCAGAACAGGTTAGATAATTTTTTTGT‑CGC‑TTAATCCCTAA‑AAAA‑CATGCAT‑GACCCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACt                                                                                                                              <  2:128234/146‑1 (MQ=255)                             aCTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                           >  1:611230/1‑151 (MQ=255)                                 ttttCTGCTCTTTTTTATTTAATGCAATCTTCTTGCTGCGCAAGCTTTTTCCAGAACAGTTTAGATGATTTTTTTGT‑CGC‑TTAATGCTTGT‑AAAA‑CATGAAT‑GACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAgc                                                                                                                        <  1:79684/150‑1 (MQ=255)                                     cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                    >  2:770711/1‑150 (MQ=255)                                         tcttttttATTTCATGAATTCTTCTGGCTGCGCAAGCTTTTCCAAGAACAGTTTAGATGATTTTTTTGT‑CGT‑TTAATGCCTGA‑AAAA‑CATGCAT‑GACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                    <  2:401157/143‑1 (MQ=255)                                         tCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtc                                                                                                               >  1:443139/1‑151 (MQ=255)                                              ttttttTCATGCATTCTTCTTGCTGCGAAAGTTTTTTCCAAAACAGGTAAGATTATTTTTTTGT‑CG‑‑TTAATTCCTTTAAAAA‑CATGCAT‑GACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                                           <  2:584503/150‑1 (MQ=255)                                               ttatttaacgaacttttcttGCTCCCCAAGC‑TTTTCCAAAAAAGGTTAAAATATTTTTTTGT‑TGCTTTAATG‑CTGA‑AAAA‑AATTGATGGACCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                          <  2:146509/135‑1 (MQ=255)                                                 aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                       >  2:288913/1‑151 (MQ=255)                                                  tttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                                      >  1:923757/1‑151 (MQ=255)                                                     cATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                   >  1:410355/1‑151 (MQ=255)                                                      aTGCATTCTTCTTGCTGCGCAAGCTTTTTCCAGAACAGTTTAGATGATTTTTTTGT‑CGT‑TTAATGCCTGA‑AAAA‑CATGAAT‑GACCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                                      <  1:348057/147‑1 (MQ=255)                                                        gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                                >  1:616252/1‑151 (MQ=255)                                                                  tgctgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                       >  2:338678/1‑150 (MQ=255)                                                                     tgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                   >  1:880046/1‑151 (MQ=255)                                                                      gcgcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                                  >  1:841465/1‑151 (MQ=255)                                                                         cAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAACTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                               >  1:471420/1‑151 (MQ=255)                                                                          aaGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                              >  1:839640/1‑151 (MQ=255)                                                                              gTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                           >  2:265953/1‑150 (MQ=255)                                                                               ttttCAAAAACAGGTAAGATTTTTTTTTTGT‑CTC‑TTAATCCCTAA‑AAAA‑CAGGCAT‑GACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                           <  2:873390/149‑1 (MQ=255)                                                                               tttcCCAAAACGGTTTAAATTTTCTTTTTGT‑CGT‑ATAATGCTAAT‑AAAA‑CATGCCT‑GAGCCAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                              <  2:546493/146‑1 (MQ=255)                                                                                     aGAACAGTTTAGATGTTTTTTTTGT‑CGC‑TAAATGCCTGA‑AAAA‑CATGCAT‑GACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                        <  1:720828/146‑1 (MQ=255)                                                                                      gAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                  >  2:842197/1‑151 (MQ=255)                                                                                       aacggtttAGATGATCTTTTTGT‑CGT‑TTAATGCCTGT‑AAAA‑CATGCAT‑GACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                  <  1:360735/144‑1 (MQ=255)                                                                                        aCAGGGTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                                >  1:717500/1‑151 (MQ=255)                                                                                           gttaagttgttttttttGT‑CTT‑TTATTCCCTAA‑AAAA‑AATGCTT‑GAGCCAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              <  2:851112/138‑1 (MQ=255)                                                                                           ggTTAGATGATTTTTTTTT‑CGT‑TTAATGCTTGA‑AAAA‑CATGCAT‑GAGCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              <  1:175811/150‑1 (MQ=255)                                                                                           ggTTAGATGATTTTTTTGT‑CTC‑TTAATGCTTGA‑AAAC‑CATGAAT‑GAGCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCa                                                               <  1:934860/149‑1 (MQ=255)                                                                                             ttAGATGTTCTTTTTGT‑CGT‑TTAATCCCTAT‑AAAC‑CTTGCAT‑GACCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                            <  2:104587/150‑1 (MQ=255)                                                                                             taagttgtttttttttT‑CGT‑TAAATGCTTGA‑AAAA‑CATGCAT‑GCCCCAAAAAATATTAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                             <  1:818178/139‑1 (MQ=255)                                                                                             gtttaatttttttttGTCCGC‑CTAACTCCTGT‑AAAACCATGCGC‑AAGCAAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATAGCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                             <  2:594552/141‑1 (MQ=255)                                                                                                 atgatTTTTTTTT‑CGT‑TTAATGCCTGA‑AAAA‑CATGCAT‑GACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                        <  1:842574/150‑1 (MQ=255)                                                                                                  tgatTTTTTTGT‑CGC‑TTAATGCCTTA‑AAAA‑CATGCAT‑GAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                       <  1:933054/150‑1 (MQ=255)                                                                                                   gatCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                     >  1:904893/1‑151 (MQ=255)                                                                                                      ttttttggcccc‑TTAATGCATTA‑AAAA‑TATGGGA‑GCGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGa                                                           <  1:532262/132‑1 (MQ=255)                                                                                                        ttttGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAGATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                                >  1:364293/1‑151 (MQ=255)                                                                                                          ttGT‑CTT‑TTAATGCCTAA‑AAAA‑AATGCAT‑GACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                               <  2:752396/150‑1 (MQ=255)                                                                                                             t‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                           >  2:380174/1‑151 (MQ=255)                                                                                                                gC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                          >  1:50378/1‑150 (MQ=255)                                                                                                                 tttaaagcccTGA‑AAAA‑CATGAAG‑GACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                            <  2:568638/140‑1 (MQ=255)                                                                                                                     aaTGCCTGA‑AAAA‑CATGAAG‑GACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                     <  2:1024969/151‑1 (MQ=255)                                                                                                                      attccctaaaaaa‑CTTGCGT‑GAGCCACAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                            <  2:170915/135‑1 (MQ=255)                                                                                                                      aTGCCTGA‑AAAA‑CATGCAT‑GACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                    <  1:24060/151‑1 (MQ=255)                                                                                                                       tGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                   >  1:633103/1‑151 (MQ=255)                                                                                                                        gCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTTGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                   >  2:657061/1‑150 (MQ=255)                                                                                                                        gCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                   >  1:1035345/1‑150 (MQ=255)                                                                                                                          cTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAA‑TTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                                         >  1:505713/1‑109 (MQ=255)                                                                                                                            gT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAACGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                               >  1:544908/1‑150 (MQ=255)                                                                                                                               aaaa‑CTTGCAT‑GACCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                             <  1:586385/150‑1 (MQ=255)                                                                                                                                aaa‑CATGCAT‑GACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                           <  2:952266/151‑1 (MQ=255)                                                                                                                                    acgtaaatggaccAAAAAAAAATAAAAAAAGGCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                         <  2:416167/140‑1 (MQ=255)                                                                                                                                      tgcag‑gAGCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                        <  1:871364/149‑1 (MQ=255)                                                                                                                                         at‑gAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     >  1:63224/1‑149 (MQ=255)                                                                                                                                         at‑gACCCACAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                       <  1:1000557/147‑1 (MQ=255)                                                                                                                                          aggaccaaaaaaaaaaaaaaaaaaaCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                   <  2:443117/142‑1 (MQ=255)                                                                                                                                           tgaccaaaaaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                    <  1:119660/141‑1 (MQ=255)                                                                                                                                              cccAAAAAAAAATATAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                      <  2:478784/143‑1 (MQ=255)                                                                                                                                               caaaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtgt                <  1:308834/145‑2 (MQ=255)                                                                                                                                                 acaAAAAAAAATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa               <  1:506639/148‑1 (MQ=255)                                                                                                                                                   aaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc          <  2:900965/151‑1 (MQ=255)                                                                                                                                                   aaaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc          <  1:927164/151‑1 (MQ=255)                                                                                                                                                    aaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         <  2:616222/151‑1 (MQ=255)                                                                                                                                                    aaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         <  2:823240/151‑1 (MQ=255)                                                                                                                                                       taataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg      >  1:175821/1‑151 (MQ=255)                                                                                                                                                          tataAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg     >  1:157553/1‑149 (MQ=255)                                                                                                                                                           ataAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAgg  >  1:492340/1‑151 (MQ=255)                                                                                                                                                            |                                                                                                                                                      TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGT‑CGC‑TTAATGCCTGT‑AAAA‑CATGCAT‑GAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑