Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,589	0	A	T	44.4%	69.2 / 27.9	54	L135F (TTA→TTT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (8/22);  new base T (23/1);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.72e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC  >  NC_000913/2151443‑2151735                                                                                                                                                    |                                                                                                                                                   aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTg                                                                                                                                                 <  1:441805/150‑1 (MQ=255)  tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATCCTTTTTTTGt                                                                                                                                               >  1:795489/1‑151 (MQ=255)   gTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGt                                                                                                                                               <  2:413122/150‑1 (MQ=255)         tGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTa                                                                                                                                         <  2:175367/150‑1 (MQ=255)             gTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACATTGATTCTTTTTTTGtggac                                                                                                                                           >  2:286811/1‑140 (MQ=255)              tAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCctttattctttatttttctcc                                                                                                                                           >  2:456523/1‑136 (MQ=255)                  cAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ACCAATTTTCCTTTTTTTGTGAccccttccaaaaa                                                                                                                                >  1:998301/1‑139 (MQ=255)                  cAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACATTTTTCCTTTTTTTGTTACCTATCCcaaaaa                                                                                                                                >  1:259254/1‑148 (MQ=255)                  cAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCCATTATCCCTTTTTTGTTACCTATTCCAaaga                                                                                                                                >  1:727095/1‑150 (MQ=255)                  cAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACCTTTTTCCTTTATTGGtgatcatctcacaa                                                                                                                                  >  2:457322/1‑135 (MQ=255)                           cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCcgac                                                                                                                      <  2:74204/151‑1 (MQ=255)                               ggTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCcgacga                                                                                                                    <  2:133350/149‑1 (MQ=255)                                     gTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTATACTTTTTTTGTGACCTCTACCAAAAAAAAAGACGACGAGCCaa                                                                                                               >  2:907118/1‑148 (MQ=255)                                       tACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACCTTATTTCTTTTTTTGTTCCCTATCCAAAAAAAAAAGACGACGAGGTaa                                                                                                               >  1:642784/1‑146 (MQ=255)                                          aaaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCaaaa                                                                                                       <  2:795450/151‑1 (MQ=255)                                            aCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACATTTTTTCTTTTTTTGTTACCTATACCAAAAAAAAAAACGACCAGCTAAGAGCaaaaa                                                                                                      >  2:421207/1‑149 (MQ=255)                                             cGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATCCTTTTTTTGTTACCTATCCCAAAAAAAAAACCGACGAGGTAAAAGCaaaaat                                                                                                     >  1:231168/1‑148 (MQ=255)                                               tAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ACAATTTTTTCTTTTTTTGTTTCCTATCCAAAAAAAGAAACCGACCGGCTAAAAAGAAAATTAGt                                                                                                  >  2:861364/1‑151 (MQ=255)                                                aGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGtt                                                                                                 <  1:555396/151‑1 (MQ=255)                                                aGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGtt                                                                                                 <  1:335691/151‑1 (MQ=255)                                                 gAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTc                                                                                                <  1:578131/151‑1 (MQ=255)                                                  aaTATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ACCATTTATTCTTTTTTTTGTACCTATTCAAAAAAAAAAAAAGACGGGCTAAAAGCAAAAAAAGttt                                                                                                >  2:330119/1‑149 (MQ=255)                                                       ggCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAAt                                                                                          <  2:978431/151‑1 (MQ=255)                                                               gTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGtt                                                                                  <  2:767529/151‑1 (MQ=255)                                                                 ggAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTtat                                                                                <  2:630273/151‑1 (MQ=255)                                                                  gAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACCTTTATTCTTTTTTTGTTACCCCTTCAAAAAAAAAAAACGACGAGGGAAAAGCAAAAATAGTTTTCTTATCAAcccgtat                                                                                >  1:961376/1‑145 (MQ=255)                                                                    aaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTTATTCTTTTTTTTGCACCTATCCAAAAAAAAAAGCCGGCCGGCGAAGAGGAAAAATAGTTTTCTAATCAGCCggtttttt                                                                             >  1:372188/1‑144 (MQ=255)                                                                    aaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑AACCTTATTTCTTTTTTTGTTCCCCATTCAAAAAAAAAAGAAGACGAACTAAAAAGAAAAATATTTTGCttaacaacccgt                                                                                  >  2:123041/1‑135 (MQ=255)                                                                     aaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑TACATTTATTCTTTTTTTGTGTCCTATCCAAAAAAAAAAACAGACGAGCTAAAAGCAAAAATAGTTTTCTTATCAGCCGGTAtattt                                                                            >  2:620763/1‑151 (MQ=255)                                                                        cAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtatttatt                                                                         <  1:73692/151‑1 (MQ=255)                                                                         aagaaagaAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtatttatt                                                                         <  2:602248/150‑1 (MQ=255)                                                                            aaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ACCAATTTTTCTTTTTTTGTCACCCATCCAAAAAAAGAAGACGACGAGGTAAGAGGAAAAATAAATTGCTAATCAACCGTTATAtttttttttt                                                                     >  1:977391/1‑151 (MQ=255)                                                                            aaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TCCAATTGTTATTTTTTTGTTTCCCCTCCAAAAAAAAAAAAAGAAGAGCGTAAATCAAAAATAGTTTGCTTATAAACCGATATATTTATTGtt                                                                      >  1:375471/1‑150 (MQ=255)                                                                             aagaagATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTAACCATTTTTCTTTTTTTGTTAACCT‑TCCAAAAAAAAAAAGCGACGAGATAAAAGGAAAAATAATTTGCTAAACAGCCCGTTTATTTAttttttt                                                                    >  1:358846/1‑150 (MQ=255)                                                                              agaagaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑AACATTTTTTCCTTTTTTGTTACCCATTCAAAAAAAAAAAACGACGAGGTAAGAGGAAAAATAATTTGCCAATAAAACGTTATAtttttttttt                                                                     >  1:981627/1‑149 (MQ=255)                                                                                 agaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCCCTGATTCTTTATTTGTTACCTATCCCAAAAAAAAAACCGACGAGCTAAGAGAAAAAAAAATTTGCTAATCAACCGGTATATTTTTTGTTTtaaa                                                                 >  1:177504/1‑149 (MQ=255)                                                                                 agaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACt                                                                <  1:248430/151‑1 (MQ=255)                                                                                 agaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACt                                                                <  2:59475/151‑1 (MQ=255)                                                                                     atTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑TTCATTTTTTCTTTTTTTGTGTCCTATCCCAAAAAAGAAGGCGACGAGGTAAAAGCAAAAATATTTTGCTTATCCGCCGGTATATTTTTTTTTTtaaaagg                                                              >  2:243019/1‑145 (MQ=255)                                                                                     atTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTGT‑AACATTTATAACTTTTTTGTTACCTATCCCAAAAAAAAAGGCGAACAGGTGAAAGGAAAATTAAGTTGCTAATAAACCGGTATATTTTTTTTTTtaaattggg                                                            >  2:971947/1‑145 (MQ=255)                                                                                         cAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGtt                                                         <  1:203277/150‑1 (MQ=255)                                                                                          aGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGa                                                       <  2:739699/151‑1 (MQ=255)                                                                                             cTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑TACATTGATTTTTTTTTGGGGCGCCATCCAAAAAAAGAAAGCCACCAGCAAACAGGCAAAAAAATTTGCTAAATAGACGCTATATTTATTTTTTtaaatctggg                                                           >  2:384908/1‑137 (MQ=255)                                                                                              tGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTT‑ACCCTTTATTCTTTTTTTGGGACCTATCCAAAAAAAGAAAACGGCGAGCTAAAAGCAAAAATAATTTGCTTAATAACCCTTTTATTTATTTTTTAAAATGGCgg                                                           >  2:258269/1‑143 (MQ=255)                                                                                                          cgcgGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAAcc                                       <  1:959181/151‑1 (MQ=255)                                                                                                           gcgGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCg                                      <  2:977346/151‑1 (MQ=255)                                                                                                                            ggcgtTTAT‑ACCATTGATCTTTTATGGGTCCCCTACCAAAAAGAAGAAGCCGACGAGCAAAGAAAAAAATTATTCCGCTAATCAGCCTTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAactc                         <  2:579230/147‑1 (MQ=255)                                                                                                                             gcgtTTAT‑ACCATTGATCCTTTTTTGTACCCCTACCAAAAAAAAGAAGCCGACGAGATAAGAGAAAAATTATTCCGCTAACCACCCTTTATATTTTTTGTTTAAACTGGCGTTTGAATTGTTGTCTTAAACCGCCATGAGAAACTCActct                    <  2:882856/151‑1 (MQ=255)                                                                                                                                   at‑aCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCcac              >  1:965157/1‑151 (MQ=255)                                                                                                                                      aCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAAACGCCATGAGAAAATCACTCTGCCcac              >  1:520473/1‑149 (MQ=255)                                                                                                                                        cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTTTTCAACCCCCATGAGAAAATCACTCTGccc                >  2:277616/1‑145 (MQ=255)                                                                                                                                          ttGATCCTTTATTGGTCACCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAACCAGCCGTTATATTTATTGTTTAACCTGGCGGTTGATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAgcgc         <  1:907160/150‑1 (MQ=255)                                                                                                                                             aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCTACCGCACTGAGAAAATCAATCTGCCCACAGCGCCCGt     >  2:248656/1‑151 (MQ=255)                                                                                                                                               ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAAACGCCATGAGAAACTCACTCTGCCCACAACGCCCGTg    >  2:145576/1‑150 (MQ=255)                                                                                                                                                  ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc  >  1:500367/1‑149 (MQ=255)                                                                                                                                                    aTTGGTCCCCTACCAAAAAAAAGAAGCCGACGAGTAAAGAAAAAATTTAGTCCGCAAACCCGCCGTTATTTTTATTGTTAAAACGGGCGTTAAAATTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCa       <  2:727057/142‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   ATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTAT‑ACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCC  >  NC_000913/2151443‑2151735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑