Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	47.5%	51.4 / 40.3	61	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (1/31);  new base T (29/0);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.38e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206294‑3206584                                                                                                                                              |                                                                                                                                                       tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaa                                                                                                                                                >  1:339623/1‑150 (MQ=255)  cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaa                                                                                                                                                >  1:507941/1‑149 (MQ=255)       aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaa                                                                                                                                         <  2:100933/151‑1 (MQ=255)         cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAACaaaaa                                                                                                                                        >  2:565580/1‑150 (MQ=255)          aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAaaaaaaaaaaaaa                                                                                                                                       >  2:696586/1‑149 (MQ=255)            tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTg                                                                                                                                     <  1:601485/150‑1 (MQ=255)                aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGA‑g                                                                                                                                 >  2:335285/1‑149 (MQ=255)                  ttccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAAGATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑Gtt                                                                                                                               <  2:804905/147‑1 (MQ=255)                  tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGATTTTTTCAGACAaaaaaaaaaagctga                                                                                                                                   >  1:280559/1‑141 (MQ=255)                       gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCatg                                                                                                                         <  1:984635/150‑1 (MQ=255)                           tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAAAAAAAACTTGA‑GTTGCCatgatg                                                                                                                      >  1:414193/1‑149 (MQ=255)                            ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGc                                                                                                                   <  2:334765/151‑1 (MQ=255)                            ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGc                                                                                                                   <  2:54927/151‑1 (MQ=255)                               gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGaa                                                                                                                <  1:794757/151‑1 (MQ=255)                                 aaTATTCATTCTTATTTTCGCGTTGCTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGG‑GGTTCGATGATGAgggga                                                                                                                >  1:724159/1‑145 (MQ=255)                                   tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCACACAAAAAAAAAAAATGGA‑GTTGCCCTGATGAGCGaa                                                                                                                >  1:157002/1‑147 (MQ=255)                                    attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGA‑GGTTCCATGATGAggggaaata                                                                                                            >  1:443518/1‑142 (MQ=255)                                      tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGtaat                                                                                                          <  2:697949/150‑1 (MQ=255)                                       cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGtaata                                                                                                         <  1:665033/150‑1 (MQ=255)                                       cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGA‑GTTGCCATGATGAGCGAAAGtaaaa                                                                                                         >  1:171108/1‑148 (MQ=255)                                        attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAg                                                                                                       <  1:1029481/151‑1 (MQ=255)                                          tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGG‑GTTTCCATGATGAGGGAAAAtaataa                                                                                                        >  2:367229/1‑148 (MQ=255)                                              aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCACACAAAAAAAAAAAATGGG‑GTTTCCATGATGGGCGAAAATAAAAAGCaa                                                                                                    >  2:503745/1‑148 (MQ=255)                                                 ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACTGGG‑GTTGCCATGATTAGCGGAAGTAATAAGCAACa                                                                                                  >  1:882081/1‑147 (MQ=255)                                                  tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                              <  1:1025604/150‑1 (MQ=255)                                                     cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                          <  2:977282/151‑1 (MQ=255)                                                      gTTGATTCACGAACTGTTAATTACATATTCACGGTTGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                         <  1:884155/151‑1 (MQ=255)                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                      <  2:443496/151‑1 (MQ=255)                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                       <  2:284224/150‑1 (MQ=255)                                                               cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACCAAAAAAAAAATTAGA‑GTTTCCCTGGTGGGGGGAAGAAAAAAACAAAAAGCAGTGAATAAg                                                                                     >  2:97042/1‑146 (MQ=255)                                                                  aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga                                                                             <  2:602609/151‑1 (MQ=255)                                                                   cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAAAAAAAAACAAAAAATGGA‑GGTGCCATGATGAGGGAAAGTAATAAAAAACAAGCAGTGAAAAAGTTGaa                                                                                >  1:639165/1‑146 (MQ=255)                                                                         aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                      <  2:238350/151‑1 (MQ=255)                                                                           tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGCCATGATGAGCGGAAGTAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCg                                                                     >  1:299846/1‑150 (MQ=255)                                                                           tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                     <  1:616911/150‑1 (MQ=255)                                                                           tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCTTACAAAAATAAAAAATTGA‑GTTGGCCTGATGGGAGGAAGTAATAAGCAACAAGCAGTGAATAAGTTGACAGAGATTGt                                                                       >  2:1002502/1‑148 (MQ=255)                                                                               tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGA‑GTTGCCATTATGAGCGGAAGTAATAAACAAAAGGCAGTGAATAAATTGACAGAGATTGTCGCTaa                                                                 >  2:382874/1‑150 (MQ=255)                                                                                  tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                               <  1:10433/149‑1 (MQ=255)                                                                                        tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAATAAAAAAACTGGG‑GTTGGCATGAAGGGGGAAAGGAATAAGCAACAAGCAGGGAATAAATTTACAGAGAATGTCGCTAAAttttacccc                                                       >  2:913101/1‑145 (MQ=255)                                                                                          gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTAGAGGTTGCC‑TGATGAGCGGAAAAAATAAAAAACAAGCAGTGAATAAAATGACAGAGATAGTCGCTAAAttttacccc                                                       >  1:625329/1‑143 (MQ=255)                                                                                          gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGG‑GTTGGCCTGATGAGCGAAAGTAATAAAAAAAAAGCAGGGGAAAAATTGGAAGAGATTGTCGCTTACttttaccccc                                                      >  2:576399/1‑143 (MQ=255)                                                                                            aaaaaaTATAAAACCCATTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCAAGACAAATACAAAACCTGGT‑GTTGCTATAATGAGCGAAATTAAAAAGCAACAAGCGGTAAATAAGTCGACAGAGATTCTCATGAACTTTACCGCCATg                                                    <  2:1462/150‑1 (MQ=255)                                                                                            aaaaaaTATAAAACCACATTTTTAGTGGTAGATTGTGGCGGAGAATTTTTTCCGACAAATACAAAAAAAAGA‑GGTGCCCTGATGAGCGGAAGAAATAAACAAAAAGGAGGGAAAAAATTGACAGAGAATGTCGCTAACTTTACCCCCATg                                                    >  1:822609/1‑150 (MQ=255)                                                                                             aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGA‑GTTGCCCTGATGAGCGAAAGGAATAAACAACAGGCAGTGAATAAATTGACAGAGAATGTTGCTAACTTTTCCGcc                                                       >  1:568875/1‑146 (MQ=255)                                                                                                aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGA‑GGTGCCCTGATGAGGGAAAGAAATAAACAACAGGCAGTGAATAAGTTGACAGAGAATGTTGCTAACTTTTCCGCCCTGAttt                                                >  1:179689/1‑150 (MQ=255)                                                                                                     aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAACTGGA‑GTTGGCATGGTGGGGGAAAAAAATAAACAAAAAGCAGTGAAAAAATTGACAGAGATTGTCGCTAAATTTACCGCCCTGATTTTTTccc                                          >  2:660226/1‑151 (MQ=255)                                                                                                          cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAACTGGA‑GGTGCCATGAATAGGGGAAGGAATAAACAAAAAGCAGGGAAAAAGTTGAAAAAGAATGTTGCCAAATTTACCCCCCTGATTTCTAACCCaa                                       >  1:1046100/1‑149 (MQ=255)                                                                                                             aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGc                                    <  1:716350/149‑1 (MQ=255)                                                                                                               ttttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATACCCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtg                                 <  2:121431/150‑1 (MQ=255)                                                                                                                tttAGTGGTAGTTTGTGGCGGTGTATTTTTTCAGGCAAAAACAAAAAATGGG‑GGTGCCATGATGGGGGGAAGTAATAAACAAAAGGGAGGGAATAAAGTGACAGAGATTTTTGGCAACTTTATCCCCCTGATTTTTACCCGAATGCCtg                                 >  1:949815/1‑149 (MQ=255)                                                                                                                tttAGGGGTAGTTTGTGGCGGTGATTTTTCCCAAACAAATACAAAAACGGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGATATTTTTACCGCCATGATTTCTACCCGAATGCCtga                                <  1:992838/150‑1 (MQ=255)                                                                                                                 ttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                               <  1:523674/150‑1 (MQ=255)                                                                                                                    gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                            <  1:696625/150‑1 (MQ=255)                                                                                                                    gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAATTTGACAGGGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg                           <  2:869971/151‑1 (MQ=255)                                                                                                                      ggTATTTTGTGGGGGTAAATTTTTCAAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                          <  2:602719/150‑1 (MQ=255)                                                                                                                          gTTTGTGGCGGTGGATTTTTTCGGACAAAAAAAAAAAATGGG‑GTTGGGCTGATTGGGGAAAGTAATAAAAAAAAAGGAGTGAATAAATTTTCAGAGATTGTCGCTAACTTTATCGCCATGATTTTTACCCGAGTGACTGATGACGgggggg                     >  2:968483/1‑151 (MQ=255)                                                                                                                                 gCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt              <  1:336518/151‑1 (MQ=255)                                                                                                                                       aaTTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa        <  2:991551/151‑1 (MQ=255)                                                                                                                                       aaTTTTTCCAGAAAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa           <  2:615772/148‑1 (MQ=255)                                                                                                                                       aaTTTTTCCAAAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGGAGTAAAAAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa        <  2:85888/151‑1 (MQ=255)                                                                                                                                           tttCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt     >  2:964561/1‑150 (MQ=255)                                                                                                                                              ccAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  1:148294/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                       TCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206294‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑