Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 345,328 | 0 | C | A | 48.3% | 77.0 / 33.6 | 58 | intergenic (+337/‑76) | yahL/yahM | uncharacterized protein/uncharacterized protein |
Reads supporting (aligned to +/- strand): ref base C (24/6); new base A (0/28); total (24/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.79e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGC > NC_000913/345179‑345476 | aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa < 2:253202/151‑1 (MQ=255) aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa < 1:343602/151‑1 (MQ=255) aaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaa < 1:761257/151‑1 (MQ=255) aaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa > 1:302139/1‑150 (MQ=255) aaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaa > 1:707915/1‑150 (MQ=255) aaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaaa > 2:164514/1‑150 (MQ=255) aTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAAc > 1:1017919/1‑151 (MQ=255) ctcATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGcc > 1:997226/1‑150 (MQ=255) aTGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCAtt > 2:110024/1‑150 (MQ=255) tGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCAtt > 1:11410/1‑149 (MQ=255) gCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGc > 1:810432/1‑151 (MQ=255) aGATGTTTTGTGAATGTGTTGGCTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGACTCATATCAACAACCGCCATTAGCtc > 2:363823/1‑151 (MQ=255) gATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCtc > 2:891184/1‑150 (MQ=255) gttttggttgACATTAATATAAAAAAAATCATAATCCCATCATGTTTGTGGGGGTTTTTTTCTGTTTCTTACGCACCGCCCCCAAGCCTAACAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAg < 2:193198/147‑1 (MQ=255) tgttgtTTAATATTAATAAAAAAAAAATCATAATCCCATCTTGTTTGTGTGGGTTTTTTTTTGTATCTTCCGCATCGCACCCAAGCCTAAAAAAAAATATGCTGAAAGTCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAga < 2:190872/150‑1 (MQ=255) ttgtttgACATTAAAAAAAAAAAAATCATAATCCCATCTTGTTTGTGGGGGTTTTTTTCTTTATCTTACCCACCGCACTAAAGCCTAAAAAAAAATATGCTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgac < 1:741842/149‑1 (MQ=255) gttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACa > 1:768366/1‑150 (MQ=255) aTTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAgg > 1:90726/1‑150 (MQ=255) ttaataaaaaaaaaaaCATATTCCCATCATGTTTGTGGGGGGTTTTTTCTTTATCTCCCCCACCGCACCCAAGCCAAAAAAAAAATAGGCTGAAAGCCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAg < 2:181245/140‑1 (MQ=255) ataaaaaaaaCCAAAATCCCATCATTTTTGTGTGGGGTTTTTTCTTTATCTTACGCACCGCACCCAAGCCTGAAAAAAAATATGCTGAAGGGCTCAAAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAtt < 2:947013/144‑1 (MQ=255) aaaaaTAATAATCCCATCATTTTTGTGGGGGGTTTTTTCTTTATTTTCCGCACCGCACACAAGCCTGAAAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg < 1:961845/151‑1 (MQ=255) attcccATCATGTTTGTGGGGGTTTTTTTCTGTATCTTCCGCACCGCACTCAACCCTGAAAAAAAATATGTTGTAAGGTTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt < 1:406662/147‑1 (MQ=255) aaTCCCATCTTTTTTGTGGGGGTTTTTTTTTTTATTTTCCCCACCCCACCAAACCCTAAAAAAAAATATGCTGTAAGGCTAATATAAAAATCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACggg < 2:284122/148‑1 (MQ=255) atcagttttGGGGGGGGTTTTTTTTTTTTCTTCCGCACCCCACCAAAGCCTAAAAAAAAATATGTTGTAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAgg < 2:655686/144‑1 (MQ=255) tGTTTGTGTGGGGTCTTTTCTTTATCTTCCGCACCGCACTCACGCCGGACAAAAAATATGCTGTAAGCCTCAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTc < 2:30150/150‑1 (MQ=255) tttGTGTGGGTTTTTTTCTGTATCTTCCGCACCCCACCCAAGCCAAACAAAAAATATGCTGAAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCg < 2:43858/149‑1 (MQ=255) tttGTGGGGGTTTTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCg < 1:886221/149‑1 (MQ=255) tttGTGGGGGGTTTTTTCTGATTCTTACCCACCCCACTAAACCCAAAAAAAAATTAGGCTGTAGGGTTCATTAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTca < 1:324261/149‑2 (MQ=255) tgtgGGTTTTTTTCTATATCTTCCGCCCCGCACAAAACCCAGAAAAAAAAAATTCTGTAGGGCTCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATgg < 2:464781/149‑1 (MQ=255) ggggTTTTTTCTGTATCTTCCGCACCCCCCTCAAGCCGGAAAAAAATTATGTGGTAAGGTTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATgg < 2:464894/146‑1 (MQ=255) ggtttttttCTTTATCTTACGCATCGCACTCAAGCCAGAAAAAAAATATGCTGTAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTcc < 2:691264/146‑1 (MQ=255) tCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAAc > 1:660403/1‑151 (MQ=255) cTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACt > 2:709414/1‑151 (MQ=255) ttttttttATCTTACGCACCCCCCTCAAGCCTGACAAAAAATATGCTGTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACt < 2:201165/150‑1 (MQ=255) ttttCTGAATCTTACGCACCCCACACAAGCCTGAAAAAAAATAGGCTGAAGGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCa < 2:1017870/147‑1 (MQ=255) tttCTGTATCTTACGCACCGCACTCAAGCCTGACAAAAAATATGCTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACtt < 2:678049/150‑1 (MQ=255) gTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaa > 2:188475/1‑150 (MQ=255) ttACGCATCGCACCCAACCTTGAAAAAAAATTTGTTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAAct < 1:863386/149‑1 (MQ=255) tACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtctt > 2:841249/1‑151 (MQ=255) gCATCGCCCTCAACCCTGAAAAAAAAAATGCGGTAAGGCTAAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAActc < 1:616894/146‑1 (MQ=255) acccccctaaacccTGAAAAAAAATATCCTGAAGGACTAATACAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGa < 1:416313/138‑1 (MQ=255) aTCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGa > 1:178564/1‑150 (MQ=255) gCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc > 1:796579/1‑150 (MQ=255) aCTCAAGCCAGAAAAAAAATATGCTGTAAGCTTCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCa < 2:268883/149‑1 (MQ=255) ctaaaGCCTGAAAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGa < 1:164524/147‑1 (MQ=255) cAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcc > 1:239221/1‑151 (MQ=255) gCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCt > 2:599456/1‑151 (MQ=255) cTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAACAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCtt > 2:830151/1‑150 (MQ=255) tGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttat > 1:414802/1‑151 (MQ=255) aaaaaataattagTAAGTCTCTTATCAAATACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCATATCCCCttatt < 2:715703/136‑1 (MQ=255) atatGCTGTAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATccc < 2:422868/151‑1 (MQ=255) tatGCTGAAGGTCTAATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATccc < 2:733035/150‑1 (MQ=255) atGCTGAAGGGCCCAAAGAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCt < 1:727624/150‑1 (MQ=255) gTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaa > 1:703599/1‑149 (MQ=255) aaGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGt < 2:1020936/150‑1 (MQ=255) aaGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTg > 2:883629/1‑151 (MQ=255) aaGGCTCAAATCAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAg < 1:145496/149‑1 (MQ=255) ggCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTgg < 1:978065/150‑1 (MQ=255) cAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGc > 2:56472/1‑149 (MQ=255) | AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTGC > NC_000913/345179‑345476 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |