Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,168,895	C→T	intergenic (‑63/‑178)	ycfQ ← / → bhsA	repressor for bhsA(ycfR)/biofilm, cell surface and signaling protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,168,895	0	C	T	100.0%	241.5 / NA	63	intergenic (‑63/‑178)	ycfQ/bhsA	repressor for bhsA(ycfR)/biofilm, cell surface and signaling protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/33);  total (30/33)

ATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGTAATTGCT  >  NC_000913/1168755‑1169039                                                                                                                                             |                                                                                                                                                 aTCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGa                                                                                                                                         <  1:229056/150‑1 (MQ=255)    aaGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATccc                                                                                                                                     <  1:611738/151‑1 (MQ=255)       cgcgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCAt                                                                                                                                   <  1:320637/150‑1 (MQ=255)       cgcgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATa                                                                                                                                  <  1:583632/151‑1 (MQ=255)        gcgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATa                                                                                                                                  >  2:530118/1‑150 (MQ=255)               cccTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAaataa                                                                                                                           <  2:271325/150‑1 (MQ=255)                   gTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTg                                                                                                                        >  1:581595/1‑149 (MQ=255)                     cGAACACTTTTGGGCGGCGACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCttt                                                                                                                    >  1:529247/1‑151 (MQ=255)                     cGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTCCCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCttt                                                                                                                    <  1:405422/151‑1 (MQ=255)                       aaCACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCt                                                                                                                  >  2:720507/1‑151 (MQ=255)                       aaCACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCt                                                                                                                  >  2:436397/1‑151 (MQ=255)                         cacTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTAt                                                                                                                <  1:389587/151‑1 (MQ=255)                          acTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTAtt                                                                                                               >  2:919825/1‑151 (MQ=255)                          acTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTAtt                                                                                                               <  1:373973/151‑1 (MQ=255)                             tttGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTa                                                                                                             >  2:191582/1‑150 (MQ=255)                               tGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAAc                                                                                                           <  1:516637/150‑1 (MQ=255)                                   cggcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGaaa                                                                                                      <  1:480062/151‑1 (MQ=255)                                   cggcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGaa                                                                                                       >  2:448236/1‑150 (MQ=255)                                      ccACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAAttt                                                                                                   <  2:381949/151‑1 (MQ=255)                                       cACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAAttt                                                                                                   >  1:50723/1‑150 (MQ=255)                                        aCGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTa                                                                                                  >  2:1037646/1‑150 (MQ=255)                                           gCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGa                                                                                              <  1:532796/151‑1 (MQ=255)                                             ttttttttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGAtt                                                                                            >  2:689252/1‑151 (MQ=255)                                               ttttttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGAttt                                                                                           <  2:524826/150‑1 (MQ=255)                                                 ttttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGAttttt                                                                                         <  1:803017/150‑1 (MQ=255)                                                     acacATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaatt                                                                                    <  1:941422/151‑1 (MQ=255)                                                      cacaTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaatt                                                                                    >  1:300150/1‑150 (MQ=255)                                                       acaTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaattaa                                                                                  <  2:757696/151‑1 (MQ=255)                                                         aTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaattaatt                                                                                >  1:543836/1‑151 (MQ=255)                                                              gtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATg                                                                            >  1:351592/1‑150 (MQ=255)                                                              gtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATg                                                                            <  1:45889/150‑1 (MQ=255)                                                                tGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGAtt                                                                         >  1:908/1‑151 (MQ=255)                                                                 gAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTg                                                                        <  1:810477/151‑1 (MQ=255)                                                                   gTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTCAAAGATTTTTAAATTAATTAATGATTGtt                                                                      <  1:742557/151‑1 (MQ=255)                                                                       gTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGATATaa                                                                  <  1:761676/151‑1 (MQ=255)                                                                         tGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATaaa                                                                 <  2:393044/150‑1 (MQ=255)                                                                         tGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATaaa                                                                 >  2:352694/1‑150 (MQ=255)                                                                           ccATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATaaaaa                                                               >  2:471741/1‑150 (MQ=255)                                                                                  gCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTg                                                       >  1:392815/1‑151 (MQ=255)                                                                                   ccGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGt                                                      >  1:441034/1‑151 (MQ=255)                                                                                     gTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTAt                                                    <  1:878006/151‑1 (MQ=255)                                                                                     gTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTAt                                                    <  2:734708/151‑1 (MQ=255)                                                                                     gTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTAAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTAt                                                    <  2:16631/151‑1 (MQ=255)                                                                                       tGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTAtgt                                                  >  2:464185/1‑151 (MQ=255)                                                                                          aCCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGAt                                               >  1:198356/1‑151 (MQ=255)                                                                                             tGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATcc                                             >  2:670637/1‑150 (MQ=255)                                                                                              ggTGACTGTGAATGAAAGGTTATTATAAAAATAATCGCCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAAGTAATGATTGTTATAAAAAATATCTTGTATGTGATCCa                                            <  2:159397/150‑1 (MQ=255)                                                                                              ggTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCa                                            <  1:764209/150‑1 (MQ=255)                                                                                               gTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATcc                                             >  1:1003865/1‑148 (MQ=255)                                                                                                 gACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGAt                                         <  2:752099/150‑1 (MQ=255)                                                                                                  aCTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATca                                       <  1:2/151‑1 (MQ=255)                                                                                                        aatgaaAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCt                                  >  1:830783/1‑150 (MQ=255)                                                                                                              aGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCAttt                           <  1:529107/151‑1 (MQ=255)                                                                                                               ggTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCAttt                           >  1:158372/1‑150 (MQ=255)                                                                                                               ggTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTAGGTGATCCAGATCACATCTATCATTTa                          <  1:348484/151‑1 (MQ=255)                                                                                                                gTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCAttt                           >  1:422761/1‑149 (MQ=255)                                                                                                                 ttattaTAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGt                        >  1:142431/1‑151 (MQ=255)                                                                                                                  tattatAAAAATAATCTCCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGt                        <  2:224716/150‑1 (MQ=255)                                                                                                                        aaaaaTAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTatcga                  <  1:146004/150‑1 (MQ=255)                                                                                                                         aaaaTAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTatcgatc                <  2:50722/151‑1 (MQ=255)                                                                                                                               atCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTa            >  2:576919/1‑149 (MQ=255)                                                                                                                                tCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGt         >  1:161587/1‑151 (MQ=255)                                                                                                                                       cGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGTAAttgct  >  1:300513/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                 ATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGTAATTGCT  >  NC_000913/1168755‑1169039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑