| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,410,240 | Δ1 bp | intergenic (+289/‑40) | prmA → / → dusB | methyltransferase for 50S ribosomal subunit protein L11/tRNA‑dihydrouridine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,410,240 | 0 | T | . | 98.2% | 252.2 / ‑1.3 | 54 | intergenic (+289/‑40) | prmA/dusB | methyltransferase for 50S ribosomal subunit protein L11/tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (27/26); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
AATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGAT > NC_000913/3410101‑3410385 | aaTTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGc > 2:531863/1‑151 (MQ=255) aGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTcac > 2:272714/1‑151 (MQ=255) aTTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTAt < 2:553313/149‑1 (MQ=255) ttCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTc > 2:109734/1‑150 (MQ=255) aaTATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCAtt < 2:571404/149‑1 (MQ=255) tatGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCtt > 1:187568/1‑151 (MQ=255) atgatgATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCtt < 1:29480/150‑1 (MQ=255) tgatgaTTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTa > 2:43830/1‑150 (MQ=255) gatTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTc > 1:800226/1‑150 (MQ=255) gatTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCa < 1:145570/151‑1 (MQ=255) atTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCAt < 2:636435/151‑1 (MQ=255) tttttATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATg > 1:416811/1‑151 (MQ=255) ttttATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTaaggaag < 1:240851/150‑6 (MQ=255) ttttATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgc > 2:132697/1‑151 (MQ=255) ttATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgcg < 1:133509/150‑1 (MQ=255) tATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgcgc < 2:804780/150‑1 (MQ=255) tATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATgcgc < 1:27644/150‑1 (MQ=255) aTGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCAt < 2:55941/151‑1 (MQ=255) aTGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCAt > 1:526388/1‑151 (MQ=255) gAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCg > 2:577036/1‑151 (MQ=255) tAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGAc > 2:363936/1‑151 (MQ=255) tCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATc > 2:11401/1‑151 (MQ=255) tCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATc > 1:129214/1‑151 (MQ=255) cGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTc > 2:633932/1‑148 (MQ=255) aaaTGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGa < 1:272710/151‑1 (MQ=255) aTGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATc < 1:734065/151‑1 (MQ=255) tGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCg > 1:945228/1‑151 (MQ=255) cAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGc > 1:588414/1‑150 (MQ=255) aaTTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAg < 1:258203/151‑1 (MQ=255) aaTTCTTTGATCCATCTCAGAGGATTGGGCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGc > 1:655056/1‑149 (MQ=255) aTTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAgc > 2:869481/1‑151 (MQ=255) tCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAgcg < 2:621732/150‑1 (MQ=255) gATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCAtg < 2:919520/151‑1 (MQ=255) gATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCAt < 2:272108/150‑1 (MQ=255) gATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCAt < 1:162190/150‑1 (MQ=255) ccATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCAtggct < 1:423359/151‑1 (MQ=255) ccATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCAtggc < 2:800242/150‑1 (MQ=255) cATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCAtggctg > 1:176346/1‑151 (MQ=255) agagGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTa < 2:462199/151‑1 (MQ=255) tCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGAcc > 2:714206/1‑151 (MQ=255) aaaGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCtt > 2:406283/1‑151 (MQ=255) gTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTc > 2:305440/1‑151 (MQ=255) ttGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCg < 2:526399/150‑1 (MQ=255) ttGGCCTTTCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCg < 2:647627/150‑1 (MQ=255) tttCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTtg > 2:30297/1‑151 (MQ=255) ttCATCTCGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTtgt > 2:489805/1‑151 (MQ=255) cGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCt < 2:187569/147‑1 (MQ=255) cGTGCAAAAAATGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACg > 2:575230/1‑150 (MQ=255) aaaaTGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATggg > 1:315086/1‑150 (MQ=255) aaaaTGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGa < 1:583316/151‑1 (MQ=255) aaTGCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGc < 1:109736/151‑1 (MQ=255) gCGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCgg < 1:577025/151‑1 (MQ=255) cGTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCgg > 2:203055/1‑150 (MQ=255) gTAATA‑ACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGAt < 1:183084/151‑1 (MQ=255) | AATTTTAGTCACTATTTTCTAATATGATGATTTTTATGAGTAATTATCGCACCACGCTCATTTTAAATGCAATTCTTTGATCCATCTCAGAGGATTGGTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGAT > NC_000913/3410101‑3410385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |