Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,903,692	0	C	A	43.8%	93.8 / 52.6	64	intergenic (+318/+1055)	ygcE/queE	putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (3/33);  new base A (28/0);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 4.02e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903542‑2903841                                                                                                                                                         |                                                                                                                                                           gTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAAc                                                                                                                                                            <  1:9449/151‑1 (MQ=255)               tatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTG‑T‑TAAAACACCCATT‑AAcc                                                                                                                                               >  2:282599/1‑149 (MQ=255)                      tACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑TAAAACACTCATT‑AACCTC‑aaaaaa                                                                                                                                      >  2:612232/1‑148 (MQ=255)                           tCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTG‑T‑AAAAACACTCATT‑AACCTC‑AAAACaaaaa                                                                                                                                  >  2:795674/1‑147 (MQ=255)                            cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACa                                                                                                                                <  1:90445/150‑1 (MQ=255)                               tGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGATTTT‑T‑AAAAACACACATT‑ATTCCC‑CACACAAAAACaaaa                                                                                                                             >  1:877110/1‑149 (MQ=255)                                gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACt                                                                                                                            <  2:519006/150‑1 (MQ=255)                                gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACt                                                                                                                            <  1:66907/150‑1 (MQ=255)                                    ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTc                                                                                                                          <  2:127303/148‑1 (MQ=255)                                      aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑AAAAACACACATT‑AAACTC‑aaaaaaaaaaaaaaaattcaaa                                                                                                                      >  1:725165/1‑142 (MQ=255)                                      aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑At                                                                                                                   <  1:735981/151‑1 (MQ=255)                                       gAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑Att                                                                                                                  <  2:103100/151‑1 (MQ=255)                                        aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑AAAAACACCCACT‑ATGCTC‑CAAAAAAAAAAAAACATTC‑CG‑Attt                                                                                                                 >  2:427793/1‑151 (MQ=255)                                          tAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATT‑T‑AAAAAAACACCTT‑AAACTC‑AAAAAAAAAAAAAAAATCC‑CG‑Attt                                                                                                                 >  1:564201/1‑149 (MQ=255)                                               gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTG‑TAAAAAAAACTCATT‑A‑ACTC‑AAAACAAAAAAAAAccttcaaacttt                                                                                                                  >  1:674713/1‑134 (MQ=255)                                               gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATT‑T‑AAAAACACCCATT‑AACCTC‑AAAAAAAAAAAAAAATTCC‑AC‑ATttatt                                                                                                              >  2:851488/1‑147 (MQ=255)                                                  aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑TAAAACACACATT‑TTCCTCTAAAAAAAAAAAAAACTTcctagtttttaattaa                                                                                                           >  1:341404/1‑136 (MQ=255)                                                  aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAAc                                                                                                       <  2:674335/151‑1 (MQ=255)                                                        aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑AAAAAAAAACATT‑ATCCTC‑AAAAAAAAAAAAAAAATTC‑AA‑ATTTATTTCTTAAACa                                                                                                     >  1:387590/1‑147 (MQ=255)                                                        aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGa                                                                                                 <  2:373648/151‑1 (MQ=255)                                                          tCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑TAAAACACACATT‑ATCCTC‑AAAAAAAAAACAAAAATTC‑AG‑ATTTTTTTATTAAACAATGaa                                                                                                >  2:431305/1‑150 (MQ=255)                                                           cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATT‑T‑TAAAACACCCCTT‑AACCCC‑AAAAAAAAAAAAAAAATCC‑CGAATTTTTTATTTAACCaaaaa                                                                                                 >  2:821272/1‑146 (MQ=255)                                                                     cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTa                                                                                     <  2:698205/150‑1 (MQ=255)                                                                       aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTaaaa                                                                                  <  1:478618/151‑1 (MQ=255)                                                                         tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑TAAAACACACATT‑TACCTC‑AAAACAAAAAAAAAAATCCAAA‑ATTTTTTATTTAACAAATAaaaaaaaaaataaaaa                                                                                  >  1:488503/1‑142 (MQ=255)                                                                            cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAatat                                                                             <  2:903321/151‑1 (MQ=255)                                                                                tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATT‑T‑TAAAACACTCATT‑AACCTC‑AAAAAAAAAAAAAAAATCC‑CA‑ATTTTTTTCTTTAACAAAAAATAAAAAAATTaaaaaaaaaaat                                                                          >  1:885437/1‑144 (MQ=255)                                                                                  aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTG‑T‑AAAAACACCCATT‑AATCTC‑AAAAAAAAAAAAAAACTCC‑CG‑ATTTTTTATTTTACCAATGAAAAAAAAACTTtaaaaaaaaaatttt                                                                       >  2:207427/1‑142 (MQ=255)                                                                                   aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAAACACTCATT‑ATCCTC‑AAAAAAAAAAAAAAAATCC‑AG‑ATTTTTTTCTTTACCAAAGAAAAAAAAACTTAAAAAAAATACTG‑tt                                                                      >  2:468960/1‑150 (MQ=255)                                                                                     tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑TAAAAAACTCCTT‑AGACTC‑AAAAAAAAAACAAAATTCC‑AG‑ATTTTTTTCTTTACCCATGAAAAAAAAAAATAAAAAAAAAAATG‑TTAtt                                                                   >  1:494512/1‑151 (MQ=255)                                                                                       tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTTTA‑T‑GAAAACACTCCTTCATGTTC‑TAAAAAATGAAAAAGTTCC‑AA‑CTTTTTTAATTAACTACTG‑ATGACAAGGTTAAAAAAAATACTa‑ttatt                                                                   >  2:425917/1‑149 (MQ=255)                                                                                        gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTa                                                                <  1:22406/151‑1 (MQ=255)                                                                                         aaCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTaa                                                               <  1:112988/151‑1 (MQ=255)                                                                                             ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTG‑T‑AAAAACACACATT‑TTCCTC‑CAAAAAAAAAAAAAAATCC‑AC‑ATTTTTTTTTTTAACAAAAAAAAAAAAACTTAAAAAAAATAATG‑TTATTTTaaaaa                                                            >  2:732619/1‑148 (MQ=255)                                                                                              gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑TAAAAAAAACATT‑TTCCTC‑AAAAAAAAAAAAAACTTTC‑CA‑ATTTTTTTCTTTAACAAAAAAAAAAAAACTTAAAAAAAATAATG‑Ttttttttaaaaa                                                           >  1:400188/1‑142 (MQ=255)                                                                                              gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGaaa                                                          <  1:584951/151‑1 (MQ=255)                                                                                               atatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑TAAAAAAAACATT‑TTACTC‑AAAAAAAAAAAAAAAATTC‑CG‑ATTTTTTTTTTAAACAAAAAAAAAAAAAAATAAAAAAAAAACTG‑TTTTTTTAaaaaaa                                                          >  1:845219/1‑150 (MQ=255)                                                                                               atatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGaaaa                                                         <  1:427782/151‑1 (MQ=255)                                                                                                     ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTa                                                    <  1:178917/150‑1 (MQ=255)                                                                                                             aaGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑AAAAACACCCATT‑AGCCCC‑CAAAAAAAAACAAACTTTC‑AG‑AATTTTTTCTTTAACAATGAAAAAAAAACTTAAAAAAAAAAATG‑TTTTTTTTAAAAAAAAAATAAAAAGCAtt                                           >  2:47817/1‑151 (MQ=255)                                                                                                             aaGAACTATTCATCAACAGCGTATAGAGGCGGTTTTT‑T‑AAAAAAACACCAT‑AACCCC‑AAAAAAAAAAAAAAAATTC‑CG‑AATTTTTACTTTAACAAAGAAAAAAAAAACTAAAAAAAAAAAAA‑TTTTTTTAAAAAAAAAAATAAAAAGCAtt                                           >  1:830725/1‑151 (MQ=255)                                                                                                             aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCAt                                            <  1:795658/150‑1 (MQ=255)                                                                                                              aGAACTATTCATCAACAGCGTATAGAGGCGGTTATT‑T‑TAAAAAAAACATT‑TACCTC‑AAAAAAAAAAAAAACTTCC‑AG‑ATTTATTAATTTAACAAAGAAAAAAAAAATTAAAAAAAAAAATG‑TTTTTTTAAAGAAAAAAATAAAAAGCAtt                                           >  1:235485/1‑150 (MQ=255)                                                                                                              aGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTa                                          <  2:459081/151‑1 (MQ=255)                                                                                                               gAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTaa                                         <  2:877128/151‑1 (MQ=255)                                                                                                                aaCTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAAACACCCCTT‑TTCCTC‑CAAAAAAAAAAAAACTTCC‑CG‑ATTTTTTTATTTAACAAAGAATAAAAAACTTTAAAAATATAATG‑TTTTTTTAAAAAAAAAAATAAAAAGCAtt                                           >  2:755190/1‑148 (MQ=255)                                                                                                                  cTATTCATCAACAGCGTATAGAGGCGGATTTT‑T‑AAAAACACCCATT‑AGCCTC‑CAAACAAAAAAAAAATTCC‑AC‑ATTTTTTTCTTTAACAATGAAAAAAAAACTTAAAAAATATAATT‑TTTTTTTTAAAAAAAAAATAAAAAGCATTAAtt                                       >  2:776534/1‑150 (MQ=255)                                                                                                                    aTTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                      <  2:471941/149‑1 (MQ=255)                                                                                                                    aTTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCaa                                    <  2:510039/151‑1 (MQ=255)                                                                                                                      tcatcaACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCCTT‑TAACTC‑AAAACAAAAAAAAAATTTC‑CG‑ATTTTTTACTTTAACAAAAAAAAAAAAACTTAAAAAAAATAATT‑TTTTTTTAAAAAAAAAAATAACAAGCATTTATTTaa                                    >  2:148809/1‑149 (MQ=255)                                                                                                                      tcatcaACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat                                   <  2:281932/150‑1 (MQ=255)                                                                                                                        atcaACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATAtt                                <  2:351213/151‑1 (MQ=255)                                                                                                                        atcaACAGCGTATAGAGGCGGGTAAGTT‑AAAACCA‑TCCAT‑ATCCTC‑CAAAAAAAAAAAAAATTCC‑AG‑ATTTTTTTATTTAACAACGAAAAAAAAACCTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATAAAAAGGATTTAAttaataat                                 >  2:471608/1‑145 (MQ=255)                                                                                                                             cAGCGTATAGAGGCGGTTATT‑T‑AAAAACCCCCATT‑AACCCC‑CCAAAAAAAAAAAACTTCC‑AG‑AATTTTTAATTAAACAAAAAAAAAAAAAACTAAAAAAAAAACTT‑TTATTTTTAAGAAAAAAATAAAAAGCAATAATTCAAAATTaaaa                            >  1:248287/1‑150 (MQ=255)                                                                                                                                cGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaaa                         <  2:674729/150‑1 (MQ=255)                                                                                                                                cGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaat                        <  1:429740/151‑1 (MQ=255)                                                                                                                                   ataGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                     <  2:152621/151‑1 (MQ=255)                                                                                                                                         gCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa               <  2:769811/151‑1 (MQ=255)                                                                                                                                          cGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaa              <  1:821256/151‑1 (MQ=255)                                                                                                                                           ggTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaa             <  2:387601/151‑1 (MQ=255)                                                                                                                                               aTG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAtt          <  1:851471/150‑1 (MQ=255)                                                                                                                                                     aaaaCCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCtt      <  1:612217/150‑1 (MQ=255)                                                                                                                                                     aaaaCCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTa     <  2:341411/151‑1 (MQ=255)                                                                                                                                                      aaaCCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTa     >  1:396297/1‑150 (MQ=255)                                                                                                                                                        aCCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg  <  2:845236/151‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                           GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATG‑T‑AAAACCACTCATT‑AGCCTC‑AAAACAAAGACAAACTTCC‑AG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTG‑TTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903542‑2903841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑