Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,333,7570TG47.4% 80.3 / 15.6 57G16G (GGT→GGGispBoctaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (5/25);  new base G (27/0);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 2.32e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAATGCGC  >  NC_000913/3333613‑3333905
                                                                                                                                                |                                                                                                                                                      
cATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGtt                                                                                                                                                     >  2:418949/1‑148 (MQ=255)
 aTTTTTTCAGTACAATCACCCTATATTCCTAACCATAAGCCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTa                                                                                                                                                    <  2:114150/148‑1 (MQ=255)
     tttCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCgg                                                                                                                                             <  2:646164/150‑1 (MQ=255)
      ttCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTAATTCgg                                                                                                                                             >  2:460351/1‑149 (MQ=255)
              aaTCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATGTGGCAAACCtt                                                                                                                                     >  2:424650/1‑149 (MQ=255)
              aaTCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGCgggcaaccttt                                                                                                                                    >  2:905780/1‑141 (MQ=255)
                    ccTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATACGGCCAACCCTTagca                                                                                                                                >  2:768796/1‑148 (MQ=255)
                            ccTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTcc                                                                                                                     <  2:942351/150‑1 (MQ=255)
                               aaCCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATGTGGGAAACCCTTTTCAGCTTAAtttccac                                                                                                                  >  2:555554/1‑145 (MQ=255)
                                  cATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATTAGGCAAACCCTTGGCAGCCTaaattctcccgcc                                                                                                              >  2:736052/1‑140 (MQ=255)
                                   aTAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGCGGCAATCCTTTAGCAGCTTAAtttccccggcc                                                                                                              >  2:322364/1‑141 (MQ=255)
                                         ccTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGagca                                                                                                                                <  1:81485/127‑1 (MQ=255)
                                            aaGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGatc                                                                                                      <  2:308344/149‑1 (MQ=255)
                                             aGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGAGGGCAACCTTCAGCAACTCAATTTCTACGGCCCAATGa                                                                                                        >  1:105288/1‑146 (MQ=255)
                                                  ccTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATTCGGGGATCCCTTAGCAGCCTTATTTCCACGTCCCACTGATCCATCAAtt                                                                                              >  2:144099/1‑151 (MQ=255)
                                                    tttGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTc                                                                                                                      <  1:298112/125‑1 (MQ=255)
                                                    tttGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATTGGGGAAACCTTTTGCAGCTTTATTTCCACGTCCCACCTATCAAACAAtt                                                                                              >  1:848280/1‑149 (MQ=255)
                                                       gTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGc                                                                                          <  1:122620/150‑1 (MQ=255)
                                                        ttCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTAATGTGGCAAACCTTTTGGAGCGTAATTTCGACGGCCCAATTATCAATCAATTAgg                                                                                           >  2:305507/1‑148 (MQ=255)
                                                              gTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATGCGGCAATCCTTTAGCAGCTTTATTTCCACGTCCCACAGATCAATCAATTAGGCGAtt                                                                                      >  2:764940/1‑147 (MQ=255)
                                                                    tAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGCGGGAATCCTTTTGCAGCTTTATTTCGACGGCCAAATGATCAAACAATTAGGCTATTACATAGt                                                                               >  2:54620/1‑148 (MQ=255)
                                                                     aaTCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAg                                                                            <  2:752060/150‑1 (MQ=255)
                                                                      aTCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAgcg                                                                          <  1:261068/151‑1 (MQ=255)
                                                                       tCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATGCGGGGAACCTTTTGCAGGTTTATTTCTGCGTCCCACCAatcaaaacattatggt                                                                                         >  1:791366/1‑124 (MQ=255)
                                                                         ggggCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAgcggcg                                                                       <  1:18388/151‑1 (MQ=255)
                                                                          gggCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTAATGGGGGGAACCCTTAGAAGCCTAATTTCCACGTCCCACTGATAAAACAATTAGGCTATTACATTATCAgcggcgg                                                                      >  1:238427/1‑151 (MQ=255)
                                                                             cGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGcggcggcgg                                                                   <  1:362516/151‑1 (MQ=255)
                                                                             cGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGGTAATGAGGCAATCCCTTAGCAGCCTTAATTCCACGCCCCCCAGATCTATCAAATAGGCTATTTCATACTCAGccgcggcgg                                                                   >  1:187734/1‑151 (MQ=255)
                                                                              gAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTAATGCGGCAATCCTTTAGCAGCCTAAATTCCACGTCCCCCTCATCAAACATATAAGCTTTTTTAACTTCAGcggcggcgg                                                                   >  1:843611/1‑150 (MQ=255)
                                                                              gAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTAATGCGGCAAACCTTGAGCAGGTTTATTTCCACGTCCCACTGATCAAACAGATAGGGTATTTAATCGTCAGcggcgggggg                                                                  >  1:434554/1‑150 (MQ=255)
                                                                              gAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGAGGGAATGATTTGGGAGGCTAATTTTGACGACCCACTTATTAAACATTTATGGTTTTACATATTTAGcggtgggggg                                                                  >  1:749192/1‑150 (MQ=255)
                                                                               aaaaGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGTGGCAAACCTTTTGCAGCTTTATTTTCACGTCCAACTTATCAATTAGTTAGGGTATTTAATTGTCAGcgggggggg                                                                   >  2:588375/1‑149 (MQ=255)
                                                                                aaaGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGGTTATGCGGGAAACCTTTTGCAGCCTTATTCCCACGTCCCAATGATTAAACAGTTAGGGTATTTCATCCTCAGcggcggcgg                                                                   >  2:611714/1‑148 (MQ=255)
                                                                                  aGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAAc                                                              <  2:40530/151‑1 (MQ=255)
                                                                                       ggCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATAGGGGCAAATTTTTGCAGCGTTATTTTCACGTCCCACTCATCAAACAAATAGGGTATTTTATCGTCTGcggggggggg                                                                  >  1:96992/1‑141 (MQ=255)
                                                                                          ttttGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGGTGATACTGGAAAACTTTAAGAGCATAATTTCGACGTCCCACTTATCAATCAAGTAGGGCATTATATACTTAGcggcgggggg                                                                  >  1:550627/1‑138 (MQ=255)
                                                                                           tttGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGCGGCAAACCTTTAGCAGCTTTATTTCCACGGCCCACTCATCAATCAATTTGGGTATTAAATCCTCAGCGGCGGCGGGGAAAGTAtt                                                         >  2:59417/1‑147 (MQ=255)
                                                                                                     aTTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGc                                           <  2:696883/151‑1 (MQ=255)
                                                                                                         aGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTa                                       <  1:424639/151‑1 (MQ=255)
                                                                                                          gAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTAc                                      <  1:219214/151‑1 (MQ=255)
                                                                                                           aaaaaaTCAATGAGTTAACCGCGCAAGATATGGCGGGGGTTTATTCGGCAAACCTTTTGCAGCTTTATTTCGACGGCCCACCGATCAAATAGTTTGGCTATTTCAACGTTAGCGGCGGGGGGAAAAGGAATCGTCCGGTGGTTTCtttt                                       >  2:411920/1‑146 (MQ=255)
                                                                                                           aaaaaaTCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATTCGGCGAACCTTTCGCAGCCTAATTTCCACGGCCAACAGATCAATCAGTTAGGATATTATATAGTCAGCGGCGGGGGGGAAAGGATTTGTTCCGTGATTGATGTaa                                      >  2:882868/1‑149 (MQ=255)
                                                                                                            aaaaaTCAATGAGTTAACCGCGCAAGATATGGCGGGGGGTTATGCGGCAAAACCTTAGAAACTTAATTTTTACGGCCCACCGCTCAATTAATTATGCTATTTTAACGTCAGCGGCGGGGGGGAACGGAttttttcggtggtt                                             >  1:811022/1‑130 (MQ=255)
                                                                                                                  cAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGc                               <  2:152294/150‑1 (MQ=255)
                                                                                                                       aGTTAACCGCGCAAGATATGGCGGGGGGTTTTTCGGGCATCCTTTGGGCGGTTAATTTCCACGTCCCACTGATCAAACAGGTTGGGTATTTTATTCTCAGCCGCGGCGGGAAAAGCATTTTTCCCCTGGTTTCTGTAATGGCTGCACg                            >  2:906962/1‑148 (MQ=255)
                                                                                                                         ttAACCGCGCAAGATATGGCGGGGGGTTATGGGGGAAACCTTGAGCAGCTTTATTTCGACGGCCAACAGATCAATCAATTTGGCTATTACATCTTCAGCGGCGGGGGGGAACGGATTTTTCCGAAGATTGTTGTACTGGGTGCCCGAGGTg                       >  2:654812/1‑151 (MQ=255)
                                                                                                                             ccGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTgg                   <  1:418938/151‑1 (MQ=255)
                                                                                                                              cgcgCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGc                  <  2:811038/151‑1 (MQ=255)
                                                                                                                               gcgcAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCt                 <  1:768780/151‑1 (MQ=255)
                                                                                                                                cgcAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTa                <  2:849848/151‑1 (MQ=255)
                                                                                                                                    aGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAg            <  2:364877/151‑1 (MQ=255)
                                                                                                                                        atGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGaa        <  1:820633/151‑1 (MQ=255)
                                                                                                                                        atGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGaa        <  1:305502/151‑1 (MQ=255)
                                                                                                                                         tGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGaa        >  1:449436/1‑150 (MQ=255)
                                                                                                                                          ggCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAAt      >  1:850897/1‑151 (MQ=255)
                                                                                                                                             gggTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAATgc    <  2:791382/150‑1 (MQ=255)
                                                                                                                                              ggTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAATgcgc  <  2:155114/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                      
CATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTTGCGATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTTAATGCGGCAATCCTTGAGCAGCTTAATTCCGACGTCCAACTGATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGTAAACGTATTCGTCCGATGATTGCTGTACTGGCTGCACGAGCTGTTGGCTATGAGGGAAATGCGC  >  NC_000913/3333613‑3333905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: