Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,168,895 C→T intergenic (‑63/‑178) ycfQ ← / → bhsA repressor for bhsA(ycfR)/biofilm, cell surface and signaling protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,168,8950CT96.4% 198.1 / ‑1.1 56intergenic (‑63/‑178)ycfQ/bhsArepressor for bhsA(ycfR)/biofilm, cell surface and signaling protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (20/34);  total (21/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGTAATTGCTT  >  NC_000913/1168751‑1169040
                                                                                                                                                 |                                                                                                                                                 
ccTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTcc                                                                                                                                             >  2:44680/1‑151 (MQ=255)
ccTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTcc                                                                                                                                             >  1:146892/1‑151 (MQ=255)
   tATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTcc                                                                                                                                             >  2:686261/1‑148 (MQ=255)
   tATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCa                                                                                                                                            <  2:821433/149‑1 (MQ=255)
   tATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAg                                                                                                                                           <  1:480721/150‑1 (MQ=255)
    aTCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGa                                                                                                                                          <  2:930393/150‑1 (MQ=255)
        aGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTCACAATTGGGTTGAGTCAGTTGCCATGATGCAGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATcc                                                                                                                                       >  2:747129/1‑149 (MQ=255)
            cgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATaaa                                                                                                                                 <  2:689220/151‑1 (MQ=255)
               cATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAaat                                                                                                                              <  2:665985/151‑1 (MQ=255)
                   ccTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAAtt                                                                                                                          <  2:885541/151‑1 (MQ=255)
                   ccTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAAtt                                                                                                                          <  2:288898/151‑1 (MQ=255)
                   ccTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAAtt                                                                                                                          >  2:234166/1‑151 (MQ=255)
                      gTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTg                                                                                                                         <  1:44682/149‑1 (MQ=255)
                        cGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCtt                                                                                                                      <  1:743390/150‑1 (MQ=255)
                            cacTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTAt                                                                                                                 <  1:226628/151‑1 (MQ=255)
                                 ttGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTaa                                                                                                             <  1:386152/150‑1 (MQ=255)
                                   gggCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTg                                                                                                          <  1:316673/151‑1 (MQ=255)
                                   gggCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTg                                                                                                          >  1:193799/1‑151 (MQ=255)
                                     gcggcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGaa                                                                                                        <  1:533942/151‑1 (MQ=255)
                                     gcggcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGa                                                                                                         >  2:817068/1‑150 (MQ=255)
                                      cggcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGaaa                                                                                                       <  2:285239/151‑1 (MQ=255)
                                      cggcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGaaa                                                                                                       <  1:686245/151‑1 (MQ=255)
                                        gcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAAtt                                                                                                     >  2:811128/1‑151 (MQ=255)
                                            cGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTaaa                                                                                                 <  1:747111/151‑1 (MQ=255)
                                            cGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTaaa                                                                                                 <  1:817052/151‑1 (MQ=255)
                                                 tttttttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGAtt                                                                                             <  1:847725/150‑1 (MQ=255)
                                                     tttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTa                                                                                         >  2:789525/1‑150 (MQ=255)
                                                     tttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTa                                                                                         <  2:738534/150‑1 (MQ=255)
                                                      ttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTaa                                                                                        >  2:178610/1‑150 (MQ=255)
                                                      ttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTaa                                                                                        >  1:507377/1‑150 (MQ=255)
                                                       tACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTaaa                                                                                       <  2:264179/150‑1 (MQ=255)
                                                        acacATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaatt                                                                                     >  1:852238/1‑151 (MQ=255)
                                                         cacaTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaatta                                                                                    <  1:98993/151‑1 (MQ=255)
                                                          acaTTGTGTTGAGTCAGTTGCCATGATACCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaattaa                                                                                   <  1:217537/151‑1 (MQ=255)
                                                             aTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAaattaatt                                                                                 <  2:242948/151‑1 (MQ=255)
                                                                gtgtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATtaattaat                                                                              <  2:962589/151‑1 (MQ=255)
                                                                  gtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGa                                                                            <  2:522948/151‑1 (MQ=255)
                                                                  gtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGa                                                                            <  2:72707/151‑1 (MQ=255)
                                                                  gtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATg                                                                             >  2:139314/1‑150 (MQ=255)
                                                                    tGAGTCAGTTGTCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGAt                                                                           <  2:897070/150‑1 (MQ=255)
                                                                     gAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTg                                                                         <  2:315407/151‑1 (MQ=255)
                                                                         cAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTta                                                                      <  1:316284/150‑1 (MQ=255)
                                                                          aGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTtat                                                                     >  1:770600/1‑150 (MQ=255)
                                                                             tGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATaaa                                                                  >  1:470079/1‑150 (MQ=255)
                                                                               ccATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATaaaaaa                                                               <  1:234162/151‑1 (MQ=255)
                                                                                               ccTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATc                                               <  1:692157/151‑1 (MQ=255)
                                                                                                 tGGTGACTGTGAATGAAAGGCTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATc                                               >  1:317218/1‑149 (MQ=255)
                                                                                                                 aaGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCAtt                             <  1:340633/151‑1 (MQ=255)
                                                                                                                  aGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCAttt                            <  2:507388/151‑1 (MQ=255)
                                                                                                                  aGGTTATTATAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCAttt                            <  1:78720/151‑1 (MQ=255)
                                                                                                                     ttattaTAAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGt                         >  2:558247/1‑151 (MQ=255)
                                                                                                                          ataAAAATAATCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTatc                     <  1:718351/150‑1 (MQ=255)
                                                                                                                              aaaaaaTCACCTCCGTTCATCCGCCCAGATCCCATAAAAATACTTGAATTATATTCAAATGACATTTTAAGATTTATCACATCATTAATGCTTTCAATACCCAATATCTTTTATGTGATCCAGATCACATCTATCATTTAGTTatcgatcg                >  2:760086/1‑151 (MQ=255)
                                                                                                                               aataatCACCTCCGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGt               >  1:559229/1‑151 (MQ=255)
                                                                                                                                          ccGTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGTAAttgc    >  1:841096/1‑151 (MQ=255)
                                                                                                                                            gTTCATCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGTAAttgctt  >  2:556000/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCTTATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAAAGATTTTTAAATTAATTAATGATTGTTATAAAAAATATCTTGTATGTGATCCAGATCACATCTATCATTTAGTTATCGATCGTTAAGTAATTGCTT  >  NC_000913/1168751‑1169040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: