Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,399,666	G→A	R105C (CGC→TGC)	mreC ←	cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,399,666	0	G	A	100.0%	204.3 / NA	52	R105C (CGC→TGC)	mreC	cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

CGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACG  >  NC_000913/3399519‑3399810                                                                                                                                                    |                                                                                                                                                 cGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAg                                                                                                                                               <  1:533207/151‑1 (MQ=255)     gatgaCCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGAcgc                                                                                                                                           <  2:173660/151‑1 (MQ=255)        gaCCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGAcgcgc                                                                                                                                         <  1:570138/150‑1 (MQ=255)        gaCCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGAcgcgc                                                                                                                                         <  1:406216/150‑1 (MQ=255)             gCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTtctc                                                                                                                                   <  2:394604/151‑1 (MQ=255)                  ccTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGtt                                                                                                                              <  1:856777/151‑1 (MQ=255)                  ccTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGtt                                                                                                                              <  1:266448/151‑1 (MQ=255)                       aTAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTAt                                                                                                                         <  2:830883/151‑1 (MQ=255)                          aaCGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGt                                                                                                                      <  1:319866/151‑1 (MQ=255)                            cGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTcc                                                                                                                    >  2:617487/1‑151 (MQ=255)                                  tAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCAt                                                                                                              >  2:497082/1‑151 (MQ=255)                                  tAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCAt                                                                                                              >  1:792374/1‑151 (MQ=255)                                     cGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcag                                                                                                           <  1:4667/151‑1 (MQ=255)                                     cGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATc                                                                                                             >  1:165358/1‑149 (MQ=255)                                      gCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATcagc                                                                                                          >  2:185405/1‑151 (MQ=255)                                             ttATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCa                                                                                                    <  2:911871/150‑1 (MQ=255)                                               aTCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTg                                                                                                 >  1:622713/1‑151 (MQ=255)                                               aTCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTg                                                                                                 >  2:727269/1‑151 (MQ=255)                                               aTCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTg                                                                                                 <  2:605314/151‑1 (MQ=255)                                               aTCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTg                                                                                                 <  1:24864/151‑1 (MQ=255)                                                 cGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGt                                                                                                <  2:167060/150‑1 (MQ=255)                                                     aacaacTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGttttt                                                                                            <  2:291374/150‑1 (MQ=255)                                                           ttGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAAc                                                                                     >  2:166781/1‑151 (MQ=255)                                                             gATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAAc                                                                                     >  1:321811/1‑149 (MQ=255)                                                              aTCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACTATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGt                                                                                  >  1:835141/1‑151 (MQ=255)                                                                cGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTc                                                                                <  2:832280/151‑1 (MQ=255)                                                                   tataAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTg                                                                             >  1:327481/1‑151 (MQ=255)                                                                        ggATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTa                                                                        <  1:16700/151‑1 (MQ=255)                                                                          aTCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAAc                                                                      >  1:496237/1‑151 (MQ=255)                                                                               tAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCg                                                                 >  1:900576/1‑151 (MQ=255)                                                                                aaCCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCgg                                                                >  1:202935/1‑151 (MQ=255)                                                                                    gTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGtttt                                                            >  2:256030/1‑151 (MQ=255)                                                                                         gaTAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGt                                                       >  2:469464/1‑151 (MQ=255)                                                                                           tAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGAGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTc                                                     >  2:730531/1‑151 (MQ=255)                                                                                              ccTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTa                                                   >  2:135201/1‑150 (MQ=255)                                                                                               cTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAAt                                                 >  2:906522/1‑151 (MQ=255)                                                                                                      aCCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCAc                                          >  2:494865/1‑151 (MQ=255)                                                                                                       ccATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCAcg                                         >  2:864573/1‑151 (MQ=255)                                                                                                        cATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCAcgc                                        >  2:731584/1‑151 (MQ=255)                                                                                                         aTTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCAcgcg                                       <  1:497071/151‑1 (MQ=255)                                                                                                         aTTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCAcgcg                                       <  1:727253/151‑1 (MQ=255)                                                                                                          ttttCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCAcgcg                                       >  1:761410/1‑150 (MQ=255)                                                                                                               tGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGcc                                  >  1:523433/1‑150 (MQ=255)                                                                                                                gCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCa                                 <  2:858147/150‑1 (MQ=255)                                                                                                                gCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCa                                 <  2:165357/150‑1 (MQ=255)                                                                                                                      ccTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTg                          >  2:792509/1‑151 (MQ=255)                                                                                                                       cTGACGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGc                         >  2:779708/1‑151 (MQ=255)                                                                                                                           cGCAGCGGGGAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATa                     <  2:956249/151‑1 (MQ=255)                                                                                                                                    gAACCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCa            <  2:321818/151‑1 (MQ=255)                                                                                                                                     aaCCCAGCAGCTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATcc             >  1:945074/1‑149 (MQ=255)                                                                                                                                           gcagcTCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAAtt      <  1:617472/150‑1 (MQ=255)                                                                                                                                                tCGCACAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACg  >  1:599095/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CGCTGATGACCGGCTGGCCTTCATAAACGCCATTAACGCTACCTTTATCGATAACAACTTGATCGCTATAAGGATCGTTAACCGTGGAGATAACCTGAGTCACCATTTTCTGCTCATCCTGACGCAGCGGGGAACCCAGCAGCTCGCGCAGACGCGCGTTCTCCTGTTTGTATTGTCCAAGCATCAGCAGTTCACTGTTTTTCAGCAACAGTTCCTGACGTAACGCCCGGTTTTCAAGTTCTAATTGGTCACGCGAGGCCAGCGTCTGCGATACGCCATCCAGCAATTCACG  >  NC_000913/3399519‑3399810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑