Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,455	0	A	G	41.2%	69.5 / 11.7	51	E116G (GAA→GGA)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (9/21);  new base G (21/0);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.73e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAACGTG  >  NC_000913/2326307‑2326603                                                                                                                                                     |                                                                                                                                                     cgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaa                                                                                                                                                    <  2:578739/151‑1 (MQ=255)  gTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaa                                                                                                                                                    <  1:173883/150‑1 (MQ=255)     aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGgaaa                                                                                                                                                   >  1:268416/1‑148 (MQ=255)       gCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGa                                                                                                                                              <  1:1114120/151‑1 (MQ=255)        cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAg                                                                                                                                             <  1:136721/151‑1 (MQ=255)           tGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATTAGttt                                                                                                                                          >  2:1099204/1‑151 (MQ=255)            gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGttt                                                                                                                                          <  2:52053/150‑1 (MQ=255)            gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGtt                                                                                                                                           <  2:628923/149‑1 (MQ=255)               aaaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGAAGGGAAATATGGGTCTAGcc                                                                                                                                      >  2:190024/1‑151 (MQ=255)                 aaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGGGTTTAGcccc                                                                                                                                    >  2:309634/1‑151 (MQ=255)                   aGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCt                                                                                                                                  <  2:439573/151‑1 (MQ=255)                          tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCcactt                                                                                                                              >  1:305805/1‑148 (MQ=255)                          tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCtactt                                                                                                                              >  1:261338/1‑148 (MQ=255)                          tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGGTTAGACCCCtacttac                                                                                                                            >  2:324595/1‑150 (MQ=255)                            gCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGGAAATATGAGGTTAGCCCCCCTCTTAATc                                                                                                                          >  2:753579/1‑150 (MQ=255)                              aGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCCACTTCCTCCAt                                                                                                                       >  1:389157/1‑151 (MQ=255)                               gAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTAGCCCCCTACTTACTCGAt                                                                                                                       >  1:270198/1‑150 (MQ=255)                                aaaCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATg                                                                                                                      >  1:551755/1‑150 (MQ=255)                                 aaCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCa                                                                                                                    <  1:309503/151‑1 (MQ=255)                                      tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGGGGTTTGCCCCCTCCTTTCACGATGCaaa                                                                                                                  >  2:271554/1‑148 (MQ=255)                                      tgtgCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGGTTTACCCCCTACTTACTCGATGCaaaa                                                                                                                 >  1:9740/1‑149 (MQ=255)                                         gCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                                                                                             <  1:503904/150‑1 (MQ=255)                                            gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAAAATTAGGTTAGCCCCCCCCTTACTCGATGCAAAAACACGccc                                                                                                         >  2:879005/1‑149 (MQ=255)                                            gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCTACTTACTCGGTGCAAAAACACGctc                                                                                                         >  2:211107/1‑151 (MQ=255)                                                cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGt                                                                                                     <  2:736148/151‑1 (MQ=255)                                                cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCCACTTTCTCGATGCCAAAGCACGCTCTgg                                                                                                      >  1:145739/1‑150 (MQ=255)                                                   ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTAt                                                                                                  <  1:598773/151‑1 (MQ=255)                                                   ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGCTGCAAAAACACGCTCTGGTTAt                                                                                                  >  1:292492/1‑151 (MQ=255)                                                   ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATTAGGTTAACCCCCCACTTTCTCCATGCAAAAACAAGccccgggttt                                                                                                  >  2:537102/1‑142 (MQ=255)                                                     tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATAAGTTTAGCCCCCTACTTACTCGATGGAAAAACACGCTCTgggt                                                                                                    >  1:274951/1‑145 (MQ=255)                                                               aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAc                                                                                      <  1:980007/151‑1 (MQ=255)                                                                tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCCACTTAATCGATGCAAAAACACGCTCTGGTTATCGTCTTGGGGACg                                                                                     >  2:703935/1‑151 (MQ=255)                                                                tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGGTTTGCCCCCCACTTACTCGATGCAAAAGCCCGCCCTGGGTATCGTCTTTGGGAc                                                                                      >  2:1079438/1‑150 (MQ=255)                                                                tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGGGGTTAGGCCCCTACTTACTCCCTGCAAAAGCACGCTCTCGTTATCCTCTTgtcga                                                                                       >  2:387508/1‑145 (MQ=255)                                                                  tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATTAGTTTAGCCCCCTCCTTACTCGATGGAAAAGCACGCTCTGGTTTTTGTCTTGGGGACgg                                                                                    >  2:86313/1‑150 (MQ=255)                                                                    gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACa                                                                                 <  1:449992/151‑1 (MQ=255)                                                                    gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCCACTTACTCGATGCCAAAACACGCTCTGGTTATCGTCTTGGAGACgg                                                                                    >  1:745288/1‑148 (MQ=255)                                                                      tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAAAGAGGTTTTCCCCCCACTTTTTCGATGCAAAAACAAGCTCTGGGTTTTGTCTTGGAGACGGAAAgg                                                                               >  2:810977/1‑151 (MQ=255)                                                                       cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGt                                                                              <  1:287440/151‑1 (MQ=255)                                                                       cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATTGGTTTAAGCCCCCACTTACTCGTTACAAAAACACGCTCTGGTTATCGTCTTTGAGACGGAc                                                                                  >  1:157555/1‑147 (MQ=255)                                                                        gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGtt                                                                             <  1:274135/151‑1 (MQ=255)                                                                                       ggCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCt                                                               <  1:111610/150‑1 (MQ=255)                                                                                          gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTTGCCCCCCACTTTCTCGATGCAAAAGCACGCCCTGGTTATCGTCTTTGGGACGGGCAGGGTTATGACGTAATCCTgcg                                                            >  2:902579/1‑150 (MQ=255)                                                                                               ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATgg                                                      <  2:792318/151‑1 (MQ=255)                                                                                                 gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAAGACGCCCTGGTTATCGTCTTGGGGACGGACAGGGTTATTACGTAATCCTGCGCGATgg                                                      >  2:1081725/1‑149 (MQ=255)                                                                                                   ccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTGTTGGAAATATGTGTTTTGGCCCCTACTTAATCGATGCAAAAGCACGCTCTTGTTATCGTCTTGGGGACGGACAGGTTTATGACGTAATCCTGCGCGGTgggc                                                    >  1:93136/1‑147 (MQ=255)                                                                                                                      cAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGtt                               <  2:987099/151‑1 (MQ=255)                                                                                                                      cAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGt                                <  1:495028/150‑1 (MQ=255)                                                                                                                                           gggggTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCGCCCATGGTTATCATATGGGGATTAccgccg          <  1:281657/151‑1 (MQ=255)                                                                                                                                             gggTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaa        <  2:274945/151‑1 (MQ=255)                                                                                                                                             gggTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaa        <  1:870924/151‑1 (MQ=255)                                                                                                                                              ggTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaa        >  2:587605/1‑150 (MQ=255)                                                                                                                                              ggTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGaa        >  1:885365/1‑150 (MQ=255)                                                                                                                                                   ggAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAACGTg  <  1:438358/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAACGTG  >  NC_000913/2326307‑2326603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑