Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,337,7230AC45.8% 73.5 / 11.0 49G566G (GGT→GGG) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base A (23/3);  new base C (0/22);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 8.48e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGAC  >  NC_000913/2337576‑2337871
                                                                                                                                                   |                                                                                                                                                    
gcgcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCt                                                                                                                                                   >  2:296228/1‑151 (MQ=255)
  gcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCttt                                                                                                                                                 >  2:344792/1‑151 (MQ=255)
    gcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTcc                                                                                                                                               <  1:214537/151‑1 (MQ=255)
       ccACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGc                                                                                                                                            <  1:587603/151‑1 (MQ=255)
         aCGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGcc                                                                                                                                           >  2:1032969/1‑150 (MQ=255)
              tGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCacgacg                                                                                                                                     >  1:941451/1‑151 (MQ=255)
                            tGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTAtt                                                                                                                       >  1:741244/1‑151 (MQ=255)
                                aacttttcccgaaaTAGCCGCGCCCAGGGCTGGAAAAGCACAGAATAGGGTCGTGAGTGTTCCCCACCAGCATTCGGTTTATAAATCCTTCTTCTTAAATTCGTGCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTc                                                                                                                      <  1:581367/136‑1 (MQ=255)
                                    tttCATCAAATAGACGCGCCCGCGGCGGGAAAAGCAAAAAATATGGTCGTGATTTTTCGCCCCCAGCAGTGGGTCAATAAAGTCTTCTTTTTTAATCCGGGCGGAAAATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGaaa                                                                                                                 <  2:849125/149‑1 (MQ=255)
                                        aTCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCTCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCt                                                                                                           >  1:718636/1‑151 (MQ=255)
                                            aaTAGCCGCGACCCCGGTGGGAAAAGCAAAAAATATGTTCGGGAGTTTCCCCCCCCAGCAGTCGGTCAAAAAATTTTTCTTTTTTAATACGGGCGGCAATTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCg                                                                                                              <  1:147018/144‑1 (MQ=255)
                                                gccgggcccggggggggAGAACCTCAGAATTTGGTGGTATGTGTGCCCCGCCAACACTGGTTCGATAAGGTCTTCTTATTTAATGTGGGCGTGACTTTGCCCTTGTCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTa                                                                                                   <  1:328983/136‑1 (MQ=255)
                                                    cgcccAGGGCGGGAGAAGCACAGAATATGGTCGTGAGTTTCCCCCACCAGCAGTCGGTCAAAAAAGTCTTCTTTTTTAATGCGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAAc                                                                                                 <  1:1107931/146‑1 (MQ=255)
                                                       ccgcggcggGAGAAGCAAAAAATATGGTCGTGAGTGTCCCCCACCACCAGCCGGTCGATAAATTCTTTTTCTTTAATCCGTGCGGAAGTTTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACg                                                                                                <  2:741223/144‑1 (MQ=255)
                                                       ccccggcggGAGAAGCACAGAATATGGTCGTGAGTTTGCCCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTCTTTAATCCGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAAc                                                                                                 <  1:253198/143‑1 (MQ=255)
                                                       ccACGGCGGAAGAAGCAAAAAATTGGTTCGTAGGTGTCCCCCACCAGCGGCCGGTGAAAAAATTCTTTTTTTTTAATGCGTGCGGCAAATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt                                                                                               <  2:783627/148‑1 (MQ=255)
                                                        cACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGcc                                                                                           >  1:714542/1‑151 (MQ=255)
                                                           ggCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTg                                                                                        >  1:419523/1‑151 (MQ=255)
                                                               ggAGAAGAACAAATTATGTTGGTGAGTTTCCCCCCCCAGCGGTCGGTCAAAAAGTTCTTTTTTTTTAACACGTGCGGAAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTgg                                                                                       <  2:263900/148‑1 (MQ=255)
                                                                   aaGCACAGAATATGGTCGGGAGTGTTCGCCCCCAGCAGCCGGTCAATAAAGTTTTCTTTTTTAATACGGGGGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagaga                                                                                <  1:286401/151‑1 (MQ=255)
                                                                          gAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                                                         >  2:423470/1‑151 (MQ=255)
                                                                           aaTATGGTCGTGAGTGTCCGCCCCCAGCATTCGGTCGATAAAGTCTTCTTCTTTATTACGGGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGt                                                                            <  2:887008/147‑1 (MQ=255)
                                                                             tatGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGAc                                                                      >  2:392681/1‑151 (MQ=255)
                                                                                     tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                                              >  1:844208/1‑151 (MQ=255)
                                                                                     tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                                              >  1:421248/1‑151 (MQ=255)
                                                                                      gAGTGTTCGCCCCCAGCAGTCGGTCAATAAAGTCTTCTTCTTTAATACGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATc                                                                <  1:437391/148‑1 (MQ=255)
                                                                                        gtgtTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt                                                           >  2:528181/1‑151 (MQ=255)
                                                                                        gtgtCCCCCCCCAGCAGTCGGTCGATAAAGTTTTCTTTTTTAATACGTGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                                                              <  2:979227/148‑1 (MQ=255)
                                                                                              gCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTg                                                      >  2:573694/1‑150 (MQ=255)
                                                                                              cccccaagcggTCGGTCAAAAAATTCTTCTTTTTTAATACGTGCGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGa                                                     <  2:468948/145‑1 (MQ=255)
                                                                                               ccaccaGCAGTCGGCGGAAAAATTCTTCTTTTTTATTCCGGGGGGAAATTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTcc                                                            <  2:984017/143‑1 (MQ=255)
                                                                                                  ccaGCAGTCGGTCAATAAAGTCTTCTTTTTTAATACGGGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCa                                                  <  1:296238/150‑1 (MQ=255)
                                                                                                      cagTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCt                                             >  1:146665/1‑151 (MQ=255)
                                                                                                          cggtcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc                                          >  2:225051/1‑150 (MQ=255)
                                                                                                           ggtcgATAAATTCTTCTTTTTTAATGCGTGCGGAAGTTTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAg                                        <  2:300744/151‑1 (MQ=255)
                                                                                                           ggtcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc                                          <  2:248010/149‑1 (MQ=255)
                                                                                                              cgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGtt                                     >  2:380534/1‑151 (MQ=255)
                                                                                                                 tAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGAt                                  >  2:647439/1‑151 (MQ=255)
                                                                                                                   aattcttcttctTTAATGCGGGCGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGt                                <  2:20118/148‑1 (MQ=255)
                                                                                                                    aGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTc                               >  1:1127262/1‑151 (MQ=255)
                                                                                                                           ttcttTAATACGTGGGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgc                            <  1:403388/147‑1 (MQ=255)
                                                                                                                           ttcttAAATACGTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcg                           <  1:544600/148‑1 (MQ=255)
                                                                                                                              tttaaagtgTGCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcg                           <  2:4121/137‑1 (MQ=255)
                                                                                                                                 aaTACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCg                  >  1:1077727/1‑151 (MQ=255)
                                                                                                                                   tgggTGGGGCATTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCgg                 <  1:344803/147‑1 (MQ=255)
                                                                                                                                   tACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCgg                 >  1:855367/1‑150 (MQ=255)
                                                                                                                                       tGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGAtt            >  1:906533/1‑151 (MQ=255)
                                                                                                                                        gcgcaaaaTTTTCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGAta            <  1:1032995/143‑2 (MQ=255)
                                                                                                                                                  tACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTacgac  >  2:427551/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGAC  >  NC_000913/2337576‑2337871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑