Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,921,166 | 0 | G | A | 40.0% | 73.6 / 22.4 | 56 | S312F (TCC→TTC) | gudP | putative D‑glucarate transporter |
Reads supporting (aligned to +/- strand): ref base G (25/8); new base A (0/22); total (26/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.79e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGT > NC_000913/2921016‑2921316 | ccGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCgg < 1:427828/151‑1 (MQ=255) cGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCgg > 2:145879/1‑150 (MQ=255) ccATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTCGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAg < 2:857009/151‑1 (MQ=255) aaaGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGcaca < 1:512217/151‑1 (MQ=255) aaaGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGcac < 1:117944/150‑1 (MQ=255) aGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAcc > 1:530420/1‑151 (MQ=255) atcCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaac > 1:429712/1‑151 (MQ=255) acgtggaggtgtttacAGAACACCCTCCCCACCGGCGGTACCATCCCCATTACGTTCGGTTTTGAAGGCGTAGTGTGCGGGGTCCCCGT‑GCCGCGCACCGGCCAGTCTGAAATAATCCCCCCCACCACCCCGCCGATAaaaccgcaaacc < 2:363731/135‑1 (MQ=255) aCGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAAccgccg > 1:1126867/1‑148 (MQ=255) aCGTAGTGGCAAACCCCCACCACCATCAACAGCAACATGCCCATTCCGATCGTTTTTTTCCCCGCAATGTACAGCGACCCCGT‑GCGGCCCACCACCCAGCCGAAAAAAACCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCcgga < 2:154237/150‑1 (MQ=255) tAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg > 1:637990/1‑151 (MQ=255) aGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg > 2:897887/1‑150 (MQ=255) gTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGCTTTCCGCGCATTGTTCGGCGAGCCCGT‑GCGGCGCATCAGCCAGTCGCAATTAATCCCACCCAGCACACCGCCGATAAGCCCGCACACCGCcggaacgg > 2:801538/1‑149 (MQ=255) tGCAGACCCCCACCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGACCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgga < 1:517303/148‑1 (MQ=255) gCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGcc > 1:480470/1‑151 (MQ=255) aGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCcac > 2:991052/1‑151 (MQ=255) caccaccaccatCCACAGCAACATGCCCTTTACGCTGGTTTTTTCACGCGAAATTTACAGAAACCCCGT‑GCGGGTCATCACCCAGTCGAAAAAATCCCCCCCCAAAAACCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACaaa < 2:1061450/150‑1 (MQ=255) caccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCt > 2:564119/1‑151 (MQ=255) accatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCtt > 1:384057/1‑151 (MQ=255) ccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCttt > 1:497891/1‑151 (MQ=255) ccaccaacacCAACATCCCCATTACGATGGTTGTTTCCCGCGAAATGTCCAGCGACCCCGC‑GCGGCACATCACCCAGCCGAAAATAACCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCttt < 2:958043/145‑1 (MQ=255) gACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGa > 1:1062559/1‑150 (MQ=255) caacAACATCCCCTTTCCTCTCGTTGTTTTACGCGCATTGTCAAGCTA‑CCCGTGGCGGCCAACCACCCACCCGAAAATAACCCCACCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCa < 2:1099201/143‑1 (MQ=255) cAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc > 2:1014748/1‑151 (MQ=255) cAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGAc > 2:587649/1‑151 (MQ=255) aaaaTGCCCATTACGACCGGTGTTTTACGCGAAATGTCCAGCGACCCCGG‑GCGGCGCACCAGCCGGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGa < 1:35068/146‑1 (MQ=255) aaCATGCCCATTACGTTCGGTTTTTTCCGCGAAATGTCCAGCGACCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc < 1:1107558/147‑1 (MQ=255) aaCATGCCCATTACGATCGGTTTTTTCCGCGCAATGTTCAGCGACCCCGT‑GCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCg < 1:674935/148‑1 (MQ=255) aCATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACAt > 2:410437/1‑151 (MQ=255) cagccccATCACGACGGGTTTTTAACGCGAAATTTCCAGCCACCCCGG‑GCGCCCCACAGGCCAGCCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACAt < 2:89431/146‑1 (MQ=255) tGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATccc > 1:117331/1‑151 (MQ=255) cccATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgc > 1:1096575/1‑151 (MQ=255) cccATTACGATCGGTGTTTTACGCGAAATGTCCAGCGACCCCGT‑GCGGCGCATCAGCCAGTGGAAAATAATCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgc < 1:669783/151‑1 (MQ=255) cccATTACCACCGGTGTTTTCCGCACAATGTCCAGCGACCCCGT‑GGGGCGCATCACCCAATCGAAAATATCCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcg < 1:635858/150‑1 (MQ=255) aCGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCt > 2:146890/1‑151 (MQ=255) cGATCGGTTTTTTACGCGCAATGTCCAGCGACCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCt < 1:310582/150‑1 (MQ=255) cGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGCGCCTg > 2:506140/1‑151 (MQ=255) gctCGGTGTTTTTAGCACAATGCGCACCGCTCCGGT‑GCGGCGCAACAGCCGCTCGAAAAAAATCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCt < 2:220724/147‑1 (MQ=255) agcGGTGTTTTCCGCGCAATGTTCAGCGACCCCGT‑GCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCa < 1:679460/149‑1 (MQ=255) tttCCGCGAAGTTTTCAGCGACCCCGT‑GCGGCGCACCAGCCAGTCGAAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATa < 2:389143/150‑1 (MQ=255) ttACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATaa > 2:196677/1‑150 (MQ=255) aCGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAAcc > 2:307424/1‑151 (MQ=255) cgcgCAATTTCCAGCGACCCCGT‑GCGGCGCATCAGCCAGTGGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCg < 1:262732/151‑1 (MQ=255) tGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAcc > 2:513595/1‑150 (MQ=255) ttcaggCGACCCGGG‑GCGGCCCATCGACCAGTCGAAACCAACCCCACCCACCATGCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCa < 2:497243/145‑1 (MQ=255) tccAGCGACCCCGT‑GCGGCCCACAAGCCATCCGAAAATAACCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAg < 2:629426/148‑1 (MQ=255) cgcccccGG‑GCGGCGCATCCCCCAGTCGAAAAAAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGta < 2:530406/144‑1 (MQ=255) gATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaata > 2:839969/1‑150 (MQ=255) cgacccgggggggccCAACAGCAAGTCGAAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAGCCGGGAACCAGGtaat < 2:544963/139‑1 (MQ=255) ccccctgccggccCACCACCCAGTCGAAAATAACCCCCCCCAACACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGta < 2:637967/138‑1 (MQ=255) cccGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaataa > 2:1054842/1‑148 (MQ=255) ccaagcggcgGATAAGCGTGTTGAAAATATCCCCCCCCAGCACACCGCCGATAATACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTACCTGCACCAGATAAACCGGGAACCAGGTAATAaagaa < 1:858490/147‑1 (MQ=255) gT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaagaag > 2:100423/1‑150 (MQ=255) ggagggacaccAGCAAGCCGAAAAAAACCCCACCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagta < 2:1097040/141‑1 (MQ=255) gcATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTc > 2:547752/1‑149 (MQ=255) cAGCCAGTCGAAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGc < 1:934806/150‑1 (MQ=255) ccAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGtt < 1:182657/150‑1 (MQ=255) cggTCGAAAATAACCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaagaag < 2:556010/133‑1 (MQ=255) ggAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATAc > 1:328493/1‑149 (MQ=255) gAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGt > 2:62734/1‑151 (MQ=255) | CCGAAGAAGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGT‑GCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGT > NC_000913/2921016‑2921316 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |