Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,995,457	0	T	G	40.7%	100.0 / 10.2	60	G232G (GGT→GGG)	dapF	diaminopimelate epimerase
Reads supporting (aligned to +/- strand):  ref base T (12/23);  new base G (24/0);  total (37/23)
Fisher's exact test for biased strand distribution p-value = 6.28e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTTTTCCGGAGCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACT  >  NC_000913/3995309‑3995605                                                                                                                                                      |                                                                                                                                                         aGCGTTTTCCGGAGCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGGAGGAGGGGTTCAG‑CAAGGgtt                                                                                                                                                        >  2:302326/1‑151 (MQ=255) aGCGTTTTCCGGAGCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGttt                                                                                                                                                        <  2:585177/151‑1 (MQ=255)    gTTTTCCGGAGCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCCGGAGGGGTTCAG‑CAAGGgtt                                                                                                                                                        >  1:1098925/1‑148 (MQ=255)    gTTTTCCGGAGCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGGTTCCG‑CAAGGttt                                                                                                                                                        >  2:353220/1‑148 (MQ=255)             aGCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGGTTGCTGGcc                                                                                                                                                 >  1:1103243/1‑146 (MQ=255)              gcgcgcCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGGTTTAG‑CAAGGGTTGCTGGCCgga                                                                                                                                              >  2:99041/1‑146 (MQ=255)               cgcgcCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTTAG‑CAAGGGTTTCTGGCCgaa                                                                                                                                              >  1:1099558/1‑147 (MQ=255)               cgcgcCAATATCGGTTTTATGCAAGTGGTTAAGCGCCAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGAAGGGATTCAG‑CCAGGGTTGTTGGGCgaagaa                                                                                                                                           >  1:148954/1‑150 (MQ=255)                gcgcCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGt                                                                                                                                         <  1:608442/151‑1 (MQ=255)                  gcCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGGTTTCTGGCCGAAGAAGTa                                                                                                                                        >  1:973480/1‑150 (MQ=255)                  gcCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGGAGGGATTCAG‑CAAGGGTTGCTGGCCgaa                                                                                                                                              >  1:865882/1‑144 (MQ=255)                   ccAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGGAGTAGGGATTCAG‑CAAGGGTTGGCGGCCgaa                                                                                                                                              >  2:1047700/1‑143 (MQ=255)                   ccAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGGTGGAGTAGGGGTTTAG‑CAAGGGTTGGTGGCCggaaa                                                                                                                                            >  2:290076/1‑141 (MQ=255)                          cGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGa                                                                                                                               <  2:1108072/150‑1 (MQ=255)                           ggTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAAc                                                                                                                             <  1:1048517/151‑1 (MQ=255)                           ggTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAAc                                                                                                                             <  2:945814/151‑1 (MQ=255)                                      aGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGgt                                                                                                                  <  1:767773/151‑1 (MQ=255)                                         ggTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGgtc‑gt                                                                                                              <  2:218941/151‑1 (MQ=255)                                         ggTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGgtc‑g                                                                                                               <  1:639935/150‑1 (MQ=255)                                           ttAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGGAGTAGGGGGTCAA‑CCAGGGTTTCTGGCCGGAGAAAGACG‑CGTGGAAGTCCCccgcgg                                                                                                                   >  2:154156/1‑145 (MQ=255)                                            tAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGCAAAAATACG‑CGGGGAACTCCCCGGCGggc‑gt                                                                                                              >  2:1010253/1‑148 (MQ=255)                                               gcgcGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGGAGGGGTTCAG‑CAAGGGTTGCTGGCCGAAGAAGGACG‑CGTGGAACTCCCCGGCGGGC‑GGCtt                                                                                                           >  1:949040/1‑148 (MQ=255)                                                  cgAGCATATTCTTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGGCGGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTCTGCTGGCCGAAGAAGTACG‑CGTGGAACGCTCCGTCGGTC‑GGCtt                                                                                                           <  2:673806/145‑1 (MQ=255)                                                   gAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCg                                                                                                    <  2:1037410/151‑1 (MQ=255)                                                   gAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCg                                                                                                    <  1:915037/151‑1 (MQ=255)                                                    aGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGACTCCGTTGGGACGCCG‑CTCGGGTTCCTGGGCGAAGAAGTATGCCGTGCTA‑TCACCGGGGGGC‑GCCTTGAAATCG‑c                                                                                                  >  2:960436/1‑151 (MQ=255)                                                      cATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTTCAGTAGGGGTTCCG‑CAAGGGTTTCTGGCCGAAGAAGTACG‑CGGGGGACTCCCCGGCGGGC‑GTCTTGTTATCG‑cc                                                                                                 >  1:55975/1‑150 (MQ=255)                                                         atTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑Tgg                                                                                             <  2:592692/150‑1 (MQ=255)                                                          ttCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGGAGGGAATCAGCCAAGGTTT‑CTTGCCGGAGAAGGACG‑CGGGGAAATCCCCGGCGGGG‑GGCTTTATATCG‑CC‑Tgg                                                                                             >  2:411988/1‑149 (MQ=255)                                                            cGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGGCGTAGGGATTTAG‑CAAGGGTTTCTGGGCGCAGAAGTACG‑CGGGGAACTCCCCCGCGGTA‑GGCTTGATATCA‑cccg                                                                                               >  1:824566/1‑144 (MQ=255)                                                             gTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGGATTAG‑CAAGGGTTGTTGGCCGCAGAAGTACG‑CGGGGAACTCCCCGGCGGGC‑GTCTTGTTATCT‑CC‑CGGaa                                                                                           >  2:1082213/1‑148 (MQ=255)                                                               ttACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGt                                                                                       <  1:307622/150‑1 (MQ=255)                                                                tACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTTGAGTAGGGGTTCAG‑CAAGGGTTGCTTGCCGGAGAAGTACG‑CGTGGGACTCCCCCGCGGGC‑GTCTTGATATCGCCC‑TGGAAgggcc                                                                                      >  2:70613/1‑149 (MQ=255)                                                                   gcgTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGGTTCAG‑CAAGGTTTGGTGGGCGAAGAAGTACG‑CGTGGGACTCCCCGGCGGGC‑GGCTTGGTATCG‑CC‑TGGAAAGGGCCggg                                                                                  >  1:174558/1‑151 (MQ=255)                                                                    cgTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTTAG‑CAAGGGTTGCTGGCCGAAGAAGTACG‑CGGGGAACTCCCCGGCGGTC‑GGCTTGATATCG‑CC‑TGGaaaagggcggg                                                                                  >  1:402291/1‑142 (MQ=255)                                                                    cgTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGGTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTTATATCG‑CC‑TGGGAAAGTCCggg                                                                                  >  1:793709/1‑150 (MQ=255)                                                                     gTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGGTTTCTGGCCGGAGAAGGACG‑CGGGGAACTCCCCCGCGGGC‑GTCTTGGTATCG‑CC‑TGGGAAAGGCCggg                                                                                  >  1:934760/1‑149 (MQ=255)                                                                       ttATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTTCAGTGGGGATTCAG‑CAAAGTTTGCTTGCCGCAGAAGAAAG‑CGTGGAACTCCCCGGGCGCT‑GCATTGATTAAG‑CC‑TGGAAAGGTCCggg                                                                                  >  2:893775/1‑147 (MQ=255)                                                                         aTGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCAccc                                                                            <  1:455699/151‑1 (MQ=255)                                                                          tGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGGAGGGATTCAG‑CCAGGGTTGCTGGCCGAAGAAGGACG‑CGTGGAACTCCCCGGCGGGC‑GGCTTTATAACG‑CC‑TGGGAAGGGCCCGGGCAccc                                                                            >  2:5522/1‑150 (MQ=255)                                                                           gAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGt                                                                          <  1:461051/151‑1 (MQ=255)                                                                           gAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGAAGTAGGGGTTTAG‑CAAAGGTTTCTGGCCGAAGAAGGACC‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CCTTGGAAAGG‑GCGGGGCAccc                                                                            >  1:1091617/1‑149 (MQ=255)                                                                              cGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCCGGAAGGATTCAG‑CAAGGGTTGGTGGCCGAAGAAAGAAG‑CGTGGGAATCCCCGGCGGGC‑GGCTTTTAATTC‑CC‑TGGGAAAGGCCGGGGCAcac                                                                            >  2:426563/1‑144 (MQ=255)                                                                                   ggCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGAGTCAG‑CAAGGATTGCTGGCCGAAAAAGGACG‑CCTGGGACTCCCCCGCGGGC‑GGCTTGTTATCG‑GC‑TGGAAAGGGTCGGGTCACCCGTTatatat                                                                   >  1:602400/1‑150 (MQ=255)                                                                                       ggAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTg                                                              <  2:701126/151‑1 (MQ=255)                                                                                                cAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGGTTGCTGGCCGGAGAAGGACG‑CGGGGGACTCCCCCGCGGGCGGTCTT‑TTATCC‑CC‑TGGGAAAGGCCCGGTCACCCCGTATATATTAATGGgcccg                                                        >  2:465669/1‑146 (MQ=255)                                                                                                 aGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGt                                                    <  1:855223/151‑1 (MQ=255)                                                                                                    ccTGCGGCAGCGGCGCGTGTGCGGCGGTTGTAGGAGGGGTTCAG‑CAAGGGTTTCTTGCCGAAGAAAGACG‑CGTGGAACTCCCCGGCCGGC‑GTCTTGATATCG‑CC‑CGGGAAAGGCCGGGGGACCCGTTATATATGACCGGGCccgctgggc                                                  >  1:892049/1‑145 (MQ=255)                                                                                                        cGGCAGCGGCGCGTGTGCGGCGGTTGCAGGAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGGC‑GTCTTGATATCG‑CC‑TGGGAAAGTCCGGGGCACCCGTTATATATTACTGGCCCGGCGGGACATGt                                              >  1:433678/1‑150 (MQ=255)                                                                                                         ggCAGCGGCGCGTGTGCGGCGGTTTCAGGAGGGATTCAG‑CAAGGGTTGCTGGCCGAAGAAGTACG‑CGGGGGACTCCCCGGCGGGC‑GTCTTGGTATCG‑CC‑TGGAAAAGGCCGGGGCACCCGGTATATATGACTGGCCCCGCGGGACATGTCt                                            >  1:871154/1‑151 (MQ=255)                                                                                                          gCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTa                                           <  2:564932/151‑1 (MQ=255)                                                                                                          gCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTa                                           <  1:302337/151‑1 (MQ=255)                                                                                                                      gtgCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTa                                <  2:686975/150‑1 (MQ=255)                                                                                                                          ggcggTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCa                            >  1:434130/1‑150 (MQ=255)                                                                                                                          ggcggTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCAt                           <  1:99042/151‑1 (MQ=255)                                                                                                                                tGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGGACATGTCTACGACGGATTTATTCATCTAt                       >  1:882585/1‑149 (MQ=255)                                                                                                                                 tcAGTAGGGAGTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAAGTCCCCGGCGGAC‑GTCTTGATATAG‑CC‑TGGAAAGGTCCGGGGCACCCGTTAAATATGACTGGCCCGGCGGTACATGTATACGACGGATTTATTCATCTATGa                     >  2:74657/2‑150 (MQ=255)                                                                                                                                             cAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGGCGGATTTATTCATCTATGAAGCAACCAgggg         <  2:1010338/150‑1 (MQ=255)                                                                                                                                              aG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGga        >  2:547011/1‑150 (MQ=255)                                                                                                                                                 cAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGga        >  1:345265/1‑148 (MQ=255)                                                                                                                                                   aGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGgaagaa    >  2:987688/1‑150 (MQ=255)                                                                                                                                                   aGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGgaa       <  2:654562/147‑1 (MQ=255)                                                                                                                                                    ggTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAAc   >  2:522245/1‑150 (MQ=255)                                                                                                                                                    ggTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACt  <  2:429365/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                         AGCGTTTTCCGGAGCGCGCCAATATCGGTTTTATGCAAGTGGTTAAGCGCGAGCATATTCGTTTACGCGTTTATGAGCGTGGGGCAGGAGAAACCCAGGCCTGCGGCAGCGGCGCGTGTGCGGCGGTTGCAGTAGGGATTCAG‑CAAGGTTTGCTGGCCGAAGAAGTACG‑CGTGGAACTCCCCGGCGGTC‑GTCTTGATATCG‑CC‑TGGAAAGGTCCGGGTCACCCGTTATATATGACTGGCCCGGCGGTACATGTCTACGACGGATTTATTCATCTATGAAGCAACCAGGGGAAGAACT  >  NC_000913/3995309‑3995605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑