Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	338,074	0	G	T	41.7%	77.7 / 18.3	48	V433L (GTG→TTG)	yahF	putative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base G (9/19);  new base T (20/0);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 6.36e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAA  >  NC_000913/337926‑338220                                                                                                                                                     |                                                                                                                                                   ccGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTtgtg                                                                                                                                                  <  1:890131/151‑1 (MQ=255)     agcaTCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCg                                                                                                                                              <  1:81892/151‑1 (MQ=255)      gcaTCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCgg                                                                                                                                             <  1:866482/151‑1 (MQ=255)        aTCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATTGTGATGGGTTTTGTGCTCgggt                                                                                                                                           >  1:87974/1‑149 (MQ=255)         tCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTTTGCTCGGGtt                                                                                                                                          >  1:518196/1‑151 (MQ=255)                     tCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGCGGTCGTGATGGGTTTTTTGTTCGGGTTTGGATCGCa                                                                                                                                 >  1:246698/1‑148 (MQ=255)                         cGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGa                                                                                                                           <  1:654693/150‑1 (MQ=255)                           aCTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATcc                                                                                                                        <  1:665404/151‑1 (MQ=255)                             tttGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGCTGGATTTTTTGCTCGGGTTTGGATCGCATGAAGATCCgg                                                                                                                      >  1:988965/1‑151 (MQ=255)                               tGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGGATGGATTTTTTGGTCGGATTTGGATCGCATGAAAATCCgg                                                                                                                      >  1:157096/1‑149 (MQ=255)                                 gCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCgg                                                                                                                  <  2:211104/151‑1 (MQ=255)                                    aTGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTc                                                                                                               <  1:905898/151‑1 (MQ=255)                                      gacgacTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGGTGGGTTTTTTGCTCGGAATTGGGTCGCATGAAGATCCGGGCGGCTcc                                                                                                              >  1:1095546/1‑150 (MQ=255)                                      gacgacTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGTCGGCTcc                                                                                                              >  1:145987/1‑150 (MQ=255)                                          acTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGGTTTTGTGGTCGGGGTTTGATCGCATGAAGATCCGGTTGGGCccacc                                                                                                           >  1:737520/1‑149 (MQ=255)                                                 cAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGGTGGGTTTTTTGTTCGGATTTTGATCCCATGAAGAAACCGTAGGCCCCACCATcca                                                                                                      >  1:621047/1‑145 (MQ=255)                                                  aaTGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGGTCGGGATGGGTTTTTTGGTCGGATTTGGATCGCCTGAAGATCCGGCCGGCCCCACCATCCAGAc                                                                                                   >  1:267188/1‑149 (MQ=255)                                                          gCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAaagaa                                                                                         <  1:624933/151‑1 (MQ=255)                                                            cGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGAACGTGAGGGATTTTGTGCTCGGGTTTGGATGGCATGAAGATCCGGGCGGCCCCACCATCGAGACGGACAaagaa                                                                                         >  1:184236/1‑149 (MQ=255)                                                               aCCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTTTGCTCGGGTTTGGATCGCATGAAGATCCCGGCGGCTCCACCATCGAGACGATCAaagaa                                                                                         >  1:460052/1‑146 (MQ=255)                                                                   gatgatTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGGTGGGTTTTGTGGTCGGATTTGGATCGCATGAAGATCCCGTCGGCTCCACCACCCGGACGATCAAAGaagcgaaa                                                                                   >  2:1081191/1‑148 (MQ=255)                                                                   gatgatTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGaagcgaaagc                                                                                 <  2:625843/150‑1 (MQ=255)                                                                      gatTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATc                                                                             <  1:368209/151‑1 (MQ=255)                                                                      gatTGACCACACCAACCGCATCAGTCGCTTGATCGTAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCACCGAGACGATCAAAGATGCGAAAGCGAt                                                                              <  2:1090137/150‑1 (MQ=255)                                                                            ccccACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGGTTTTTTGGTCGGGTTTGGATTGCATGAAGATCCGGTCGGCTCCCCCCTCGAGGCGAACAAAGAAGCGAAAACGATcgcc                                                                          >  1:925431/1‑148 (MQ=255)                                                                             cccACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGAGATCGGGATGGATTTTTTGCTCGGGGTTTGGTTGCCTGGAGATTCAGGGGGCCCCCACATCCAGACGCCCAAAAAAGAGAAAGCGGTagcc                                                                          >  2:1118784/1‑147 (MQ=255)                                                                              ccaccaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGTGCGGGGTGGATTTTTTGGTTCGCTTTTGATCCCATGTACAACCGGGCGGCTCCCCCCCCCAGACGATCCAAAAAACGGAAAAGATcggc                                                                          >  2:654716/1‑144 (MQ=255)                                                                                  caACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCCTGGTGGGTTTTTTGGTCGGGGTTGGGTCGCATTAAGAACCGGTCGGCTCCACCAACGAGACGATCCAAGAAACGGAAACGATcgcccccggcga                                                                  >  2:1074570/1‑142 (MQ=255)                                                                                      cGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTTTGCTCGGATTTGGATCGCATGAAAATCCGGTCGGCTCCACCCTCGAGACGATCAAAAAAGCGAAAACGATCGCCCCTGcc                                                                    >  1:682540/1‑144 (MQ=255)                                                                                       gCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGAcg                                                            <  2:427191/151‑1 (MQ=255)                                                                                        cATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTTGTTGTGATGGCTTTTTTTCTCGGATTTTGATCGCATGAAAATCCCGCCGGCGCCACCATCCCGGAGCACAAAGAAGCGAAAGGGATAGCCGCTGCCGAAgg                                                               >  1:1112219/1‑147 (MQ=255)                                                                                        cATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGGGTGGATTTTGTGCTCGGGGTTGGGTCGCCTGAAGATCCGGTCGGCTCCCCCCTCGAGACGATCAAAGAAGCGAAAACGATCGCCGCCGCCgacggacg                                                            >  2:558210/1‑150 (MQ=255)                                                                                              tCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATTGTGATGGATTTTTTGGTCGGGATTGGTTTGCCTGAAGAACCCGTTGGCCCCACCCACCAGACGGTCTCAAAAAGGAAAACGATCGCCCCTGCCAGAGGGAGCGAggt                                                      >  2:845419/1‑148 (MQ=255)                                                                                                gCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGGTTTTTTGCTCGGGTTTTGATCGCATGAAGATCCGGTCGGCTCCACCATCCAGACGATCAAAAAAACGAAAACGATCGCCCCCGCCGGAGGACGCGAGTTGa                                                    >  1:144116/1‑150 (MQ=255)                                                                                                   tGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGAGATCGTGATGGGTTTTTTGCTCCGGTTTGGATCGCATGAAGATCCGGGCGGCTCCACCAACGAGACGATCAAAAAAGCGAAAGAGATCGCCGCTGCCCCAGGACGCGGGTTGATc                                                  >  2:1021500/1‑149 (MQ=255)                                                                                                     aTCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGGTTTTTTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCCCCCTCGAGACGATCAAAGAAGCGAAAACGATCGCCGCCGCCGGAGGACGCGAGGTGATCAtt                                               >  1:303049/1‑150 (MQ=255)                                                                                                           gagGCGCGCGATCCAGAAGTGGCGGTGATCGTGTTGGGTTTTTTGGTCCGGGTTTGAGCGCATGAAGAACCGGGCGGGCCCACCATCGAGACGATCAAAAAAAAGAAAACGATCGCCGCCGCCCGAGGACGGGAGTTGATCAATCTCGcc                                         >  2:933778/1‑150 (MQ=255)                                                                                                                   cgATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCAAGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTg                                <  2:906470/151‑1 (MQ=255)                                                                                                                     aTCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTggg                              <  1:69398/151‑1 (MQ=255)                                                                                                                               ggCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCtt                    >  1:617493/1‑151 (MQ=255)                                                                                                                                gCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCt                     <  2:1102187/149‑1 (MQ=255)                                                                                                                                gCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTg                   >  1:1008771/1‑151 (MQ=255)                                                                                                                                  ggTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGAt                 <  1:156233/151‑1 (MQ=255)                                                                                                                                       tCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGcc            >  2:278358/1‑151 (MQ=255)                                                                                                                                          tGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATc         >  2:845051/1‑151 (MQ=255)                                                                                                                                            aTGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGt       <  2:765498/151‑1 (MQ=255)                                                                                                                                              ggATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGtt      <  1:1131175/150‑1 (MQ=255)                                                                                                                                                  tttGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGaa  <  2:133553/150‑1 (MQ=255)                                                                                                                                                  tttGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGaa  <  2:685842/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCGCAGCATCAAACACACCTTCCTCGACTTTGGCGATGACGACTTCACCAATGGCAAGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAA  >  NC_000913/337926‑338220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑