Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,578,143	0	A	C	41.0%	135.1 / 28.5	78	L79V (TTA→GTA)	maeB	malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base A (43/3);  new base C (0/32);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 8.37e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGT  >  NC_000913/2577994‑2578285                                                                                                                                                           |                                                                                                                                                tggttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAAc                                                                                                                                                >  2:353437/1‑151 (MQ=255)     tCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAAcccc                                                                                                                                             >  2:572385/1‑150 (MQ=255)       gAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑Gc                                                                                                                                         >  1:945347/1‑151 (MQ=255)       gAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑Gc                                                                                                                                         >  2:461972/1‑151 (MQ=255)        aGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑Gc                                                                                                                                         >  2:175015/1‑150 (MQ=255)        aGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑Gc                                                                                                                                         >  1:26015/1‑150 (MQ=255)              gcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCAccgc                                                                                                                                    <  1:795084/149‑1 (MQ=255)               cgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGt                                                                                                                                 >  1:1018282/1‑151 (MQ=255)                  cAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCTCCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTAcc                                                                                                                              >  1:588101/1‑151 (MQ=255)                   aaCTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCg                                                                                                                             >  2:139250/1‑151 (MQ=255)                   aaCTTCAATAAATTTATCCGGGTCGAGTTCATCAACTTCAA‑TGTCAAATACATCAATTCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACTGCCGTACCg                                                                                                                             >  2:1180340/1‑151 (MQ=255)                       tCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGtt                                                                                                                           >  1:700639/1‑149 (MQ=255)                         aataatTTTGTCGGGGTCGAGTCCGTCACCTTCAA‑TGAAAAATACATCAACCCCGGCAAATTTCTA‑AAAC‑AGAACCCCCTTCCCTTCCACCCCCGTTTTGCCGGCCGGCGCGCAATTTTT‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTag                                                                                                                         <  2:343534/149‑1 (MQ=255)                         aaTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTag                                                                                                                         >  1:937267/1‑149 (MQ=255)                         aaTAAATTTGCCCGGGTCGAGTTCGTCAACTTAAA‑TGTAAAATACATCAACCCCGGCGAATTTCTA‑AAAC‑AGAACGCCCTTGCCTCCCATCCCCGGTTTGCCTGCCAGCGCGCCAATGTG‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTa                                                                                                                          <  1:634831/148‑1 (MQ=255)                             aaTTTGTCGGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAACCCCGGCGATTTTCTT‑AAAC‑AGACCCCCCTTGCCTTCCATCCCCGTTTTGCCTGCCGGCGCGCCATTGTT‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCa                                                                                                                   <  1:888390/151‑1 (MQ=255)                                    ccGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGccac                                                                                                            >  2:44149/1‑151 (MQ=255)                                      gggTCGAGTTCGTCACTTTAAA‑TGTAAAATACACCACCCCCGGCGATTTTCTA‑AAAC‑AAACCCCCCTTGCCTCCCACCCCCGGTTTGCCTGCCAGCGCCCAAATGTT‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGccacca                                                                                                          <  1:332229/151‑1 (MQ=255)                                       ggTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑TAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGccacc                                                                                                           >  1:547251/1‑149 (MQ=255)                                        gttcgattCCTCAACCTCAATTGCAAAATACACCAACCCCGGCGATTTTCAA‑AAAA‑AAACCCCCTTTGCCTCCCACCCCGGGTTTGCCTCCCGGCCCCCCATTTTT‑CCC‑TACCCCCA‑CCACCGCCGTACCGTTAGAGATCACCGCCACCAg                                                                                                         <  2:769836/145‑1 (MQ=255)                                        gTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAgg                                                                                                        >  1:154258/1‑151 (MQ=255)                                          cGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGtt                                                                                                      >  2:554799/1‑151 (MQ=255)                                           gAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGtt                                                                                                      >  1:427291/1‑150 (MQ=255)                                            aGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTa                                                                                                     >  2:1004875/1‑150 (MQ=255)                                                cGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCg                                                                                                >  2:285815/1‑151 (MQ=255)                                                   cAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc                                                                                             >  2:953776/1‑151 (MQ=255)                                                    aactaaaa‑TGTAAAATACAACAACCCCGG‑GAATTTTTTAAAAC‑AGACCCCCCTTCCTTCCCACCCCCGTTTTGCCTGCCGGCGCGCCATTTTG‑CCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCggg                                                                                              <  2:254124/143‑1 (MQ=255)                                                    aaCTTAAA‑TTTCAAATACATCACCCCCGGCAATTTTCTA‑AAAC‑AGACCCCCCTTCCCTCCCATCCCCGTTTTCCCTCCCGGCGCGCCATTGTT‑CCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCgg                                                                                               <  1:285818/148‑1 (MQ=255)                                                       ttaaa‑TGTAAAAAACATCAACCCCGGCAAATTTCTT‑AAAC‑AAAACCCCCTTCCCTCCCCTCCCCGTTTTGCCTGCCAGCGCGCCAATTTC‑CCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCg                                                                                            <  1:207663/145‑1 (MQ=255)                                                       ttCAA‑TTTAAAATAAATCACCCCCGGCGATTTTCTA‑AAGC‑AGACCCCCTTTGCTTCCCTCCCCCGGTTCGCCTGCCGGCGCGCAAAT‑TT‑TCC‑TAAACCCAGGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc                                                                                             <  1:554815/147‑1 (MQ=255)                                                        ttaattttaaaaTCCACCAACCCCGGC‑AATTTTTT‑AAACAAGACCCCCTTTCCCTCCCATCCCGGTTTTGCCGCCCAGCGCCCAATTGTT‑CCC‑AACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCgg                                                                                           <  1:706527/140‑1 (MQ=255)                                                          aa‑TGTAAAATACACCAACCCGGGCGATTTTTTA‑AAAC‑AGACCGCCCTTGCCTCCCACCCCCGTTTTGCCGCCCGGCCCGCCATTGTT‑CCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGt                                                                                          <  1:913392/147‑1 (MQ=255)                                                           aatttaaaaTACATCAACCCCGGCGATTTTCTA‑AAAC‑AGACCCCCCTTGCCTCCCATCCCCGTTTTGCCTGCCAGCGCCCCAATGTT‑GCC‑TACCCCAA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGta                                                                                         <  1:718726/142‑1 (MQ=255)                                                           a‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAtt                                                                                     >  2:574153/1‑151 (MQ=255)                                                             tgaaaaaTACATCAACCCCGGAAATTTTTTA‑AAAC‑AGACCCCCTTTCCCTCCCACCCCCGTTTTGCCTCCCAGCGCCCAATTGTT‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTatat                                                                                      <  2:423811/145‑1 (MQ=255)                                                                 aaaTCCACCAACCCCGGAAAATTTCTA‑AAAA‑AGACCCCCCTTCCCTCCCACCCCCGTTTTGCCTCCCGGCGCCCAATTTTT‑GCC‑AACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTa                                                                                 <  2:155708/150‑1 (MQ=255)                                                                 aaaTACACCACCCCGGGCGATTTTCTA‑AAAC‑AGACCGCCCTTCCCTTCCATCCCGGGTTTCCCGGCCAGC‑CCCCAATTTT‑TCCATACCCCCG‑GCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGt                                                                                  <  2:105436/149‑1 (MQ=255)                                                                      cATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCttt                                                                           >  1:1036368/1‑151 (MQ=255)                                                                         cAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTa                                                                         >  1:46664/1‑150 (MQ=255)                                                                              ccggaaattttCTA‑AAAC‑AGACCGCCCTTCCCTCCCACCCCCGGTTGGCCTCCCAGCCCCCAAATGTT‑CCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGt                                                                   <  1:863467/143‑1 (MQ=255)                                                                              ccGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGt                                                                   >  1:446914/1‑151 (MQ=255)                                                                                      tttCTA‑AAAC‑AGAACCCCCTTGCCTCCCATCCCGGTTTTGCCTGCCAGCGCGCCAATGTG‑CCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtttt                                                              <  1:1138726/148‑1 (MQ=255)                                                                                             aaaa‑AGACCCCCCTTCCCTTCCACCCCCGTTTTGCCTGCCGGCGCGCCAATGTT‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAt                                                         <  1:349456/147‑1 (MQ=255)                                                                                             aaaC‑AGACCCCCCTTCCTTCCCACCCCCGTTTTGCCTGCCAGCGCGCCAATTTT‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGa                                                          <  1:1023341/146‑1 (MQ=255)                                                                                              aaC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                     >  2:687109/1‑150 (MQ=255)                                                                                              aaC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                     >  2:427909/1‑150 (MQ=255)                                                                                                acaaaccccccTTCCCTCCCACCCCCGTTTTCCCGCCCAGCGCGCCATTTTT‑GCC‑TCCCCCCA‑GCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAg                                                   <  2:257300/143‑1 (MQ=255)                                                                                                  aaacccccctccccTCCCACCCCCGTTTTGCCGCCCAGCGCCCCATTGTT‑GCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAg                                                   <  2:231692/137‑1 (MQ=255)                                                                                                   gAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc                                                 >  2:1132466/1‑150 (MQ=255)                                                                                                    accccccTTCCCTCCCACCCCGGTTTTCCCTCCCAGCGCCCCATTGTT‑CCC‑AACCCCCA‑GCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGa                                                  <  1:542582/144‑1 (MQ=255)                                                                                                    aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACa                                                >  2:99903/1‑150 (MQ=255)                                                                                                    aaCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACa                                                >  2:924605/1‑150 (MQ=255)                                                                                                     aCGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACa                                                >  2:250710/1‑149 (MQ=255)                                                                                                          cTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCg                                         >  2:736058/1‑151 (MQ=255)                                                                                                            tGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGc                                       >  1:558217/1‑151 (MQ=255)                                                                                                              cctccccccccgggTTTCCCTGCCGGCGCCCCATTTTC‑CCC‑TCCCCCCA‑GCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCg                                         <  2:914438/135‑1 (MQ=255)                                                                                                              cctccaaTCACCGTTTTGCCGGCCAGCGCGCAAATGTT‑CCC‑AACCCCCA‑GCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCa                                      <  2:565576/144‑1 (MQ=255)                                                                                                              ccTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCa                                      >  2:672769/1‑150 (MQ=255)                                                                                                                ttCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                   >  2:142504/1‑151 (MQ=255)                                                                                                                ttCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                   >  2:701672/1‑151 (MQ=255)                                                                                                                 tCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGc                                  >  1:743529/1‑151 (MQ=255)                                                                                                                  ccaccccgggTTTCCCTGCCGGCGCGCAATTGTT‑GCC‑TACCCCCA‑CCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg                                   <  1:275014/141‑1 (MQ=255)                                                                                                                        cggttttCCCTGCCAGCGCCCCATTGTT‑CCC‑TACCCCAG‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAAGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGa                           <  1:572402/147‑1 (MQ=255)                                                                                                                          ggTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAg                          >  1:929894/1‑150 (MQ=255)                                                                                                                             ttGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTa                        >  2:815453/1‑149 (MQ=255)                                                                                                                              tgcctgccAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGc                     >  1:1184833/1‑151 (MQ=255)                                                                                                                               ccctcccggccccccAATTTT‑CCC‑AACCCCCA‑GCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTa                        <  2:91908/134‑1 (MQ=255)                                                                                                                                 ctgccAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTCGGc                     >  2:296754/1‑148 (MQ=255)                                                                                                                                 ccgcccggcccccAATGTTCCCC‑TACCCCCG‑CCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGt                         <  2:725952/134‑1 (MQ=255)                                                                                                                                   gccAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgcg                >  1:934294/1‑151 (MQ=255)                                                                                                                                     cAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc              >  1:56153/1‑151 (MQ=255)                                                                                                                                        cgcgcAAATGTT‑CCC‑AAACCCAA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAg            <  2:387243/150‑1 (MQ=255)                                                                                                                                        cgcccCAATTTC‑GCC‑AACCCCAA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgc                 <  1:667649/145‑1 (MQ=255)                                                                                                                                          cccaattGTT‑CCC‑TACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATc         <  1:353443/145‑1 (MQ=255)                                                                                                                                            ccATTGTT‑CCC‑TACCCCCA‑CCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc              <  1:948690/144‑1 (MQ=255)                                                                                                                                            caattttt‑GCC‑AACCCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCa             <  2:499679/139‑1 (MQ=255)                                                                                                                                              aaTTTT‑CCC‑TACCCCCA‑CCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg      <  2:1129471/150‑1 (MQ=255)                                                                                                                                                  tt‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCtgt  >  2:1565/1‑150 (MQ=255)                                                                                                                                                           |                                                                                                                                                TGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAA‑TGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTT‑GCC‑TAACCCCA‑GCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGT  >  NC_000913/2577994‑2578285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑