Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,200	0	G	A	43.9%	74.5 / 33.0	66	S217F (TCC→TTC)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base G (29/8);  new base A (0/29);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.50e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACT  >  NC_000913/3556050‑3556340                                                                                                                                                        |                                                                                                                                              aCCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAggg                                                                                                                                               >  2:996836/1‑151 (MQ=255)  ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGa                                                                                                                                              <  1:558302/151‑1 (MQ=255)   cAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGaa                                                                                                                                             <  2:1050609/151‑1 (MQ=255)       tgtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑c                                                                                                                                        >  1:1180711/1‑151 (MQ=255)        gtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑c                                                                                                                                        <  1:121242/150‑1 (MQ=255)          gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTc                                                                                                                                      <  2:432321/150‑1 (MQ=255)            cGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTcccc                                                                                                                                   <  1:286788/151‑1 (MQ=255)               aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGcc                                                                                                                                >  2:5435/1‑151 (MQ=255)               aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGcc                                                                                                                                >  2:240514/1‑151 (MQ=255)                   accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtg                                                                                                                            >  1:913634/1‑151 (MQ=255)                     cTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                           >  2:217990/1‑150 (MQ=255)                       cGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAg                                                                                                                         >  2:247788/1‑150 (MQ=255)                                   gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                            >  2:550507/1‑151 (MQ=255)                                          aCCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAAc                                                                                                      >  1:1128799/1‑150 (MQ=255)                                              acgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc                                                                                                 >  2:66718/1‑151 (MQ=255)                                                 aaaaaaaaGGGTTCGGTGATTTTTTCTCTTACGCCTAAAGGCGAGCCGTTATTCCCCGGCCCC‑TGGCCGCGGGGCAGTTTATAATTATTTTGTTCAGGAGGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc                                                                                                 <  2:662053/146‑1 (MQ=255)                                                   aaaaaaGCGTTCGGTATTTTTTTCCCTTCCGACTCAAAGGGAGACGTTTTTCCCCGCCCCC‑TGGCCGCGCGGCAGTTTATAAATTTTCTGTTCAGGAAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                  <  2:437434/145‑1 (MQ=255)                                                        aGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                       >  1:1139970/1‑151 (MQ=255)                                                           gtgcGGTAATATTTTCACTTTCAACTTAAGGGGAGACGTTATCCCCCGGCCCC‑GGGGCGCGGGGCGGTTTAAGAATATTCTTTACATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                      <  1:337663/146‑1 (MQ=255)                                                              gggTGATATTTTCACTTCCCACTTAAGGGGGGACGTTATCCCCCGCCCCC‑TGGTCGCGCGGCGGTTTAGAAATTTTCTGTCCA‑GGAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                   <  2:436567/148‑1 (MQ=255)                                                               gttatttttttCACTTCCAATTCAAAGCGAG‑CGGTTTTCCCCCGCCCCTTGCCCGCGCGACTGTTTATAATTTTTTTGTTCATG‑AGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                   <  2:849436/141‑1 (MQ=255)                                                                gttttatTTCCACTTCCGACTCCAAGCGGGCCGTTATCCCCCGCCCCC‑GGGCCGCGCGGCGGTTTATAAATTTTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAg                                                                                     <  2:651873/141‑1 (MQ=255)                                                                 tGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                >  1:893126/1‑149 (MQ=255)                                                                    ttttttCCTTTCCGTCTACAACCGAGACGTTATCCCCCGGCCCC‑TGGCCGCGCGGCAGTTTATGATTATTCTGTTCGTGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                             <  2:369232/147‑1 (MQ=255)                                                                      ttttCCCTTTCGCCTCAAAGCGAGACGTTATCCCCCGCCCCC‑TGGTCGCGCGGAAGGTTATAATTTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                          <  1:837064/150‑1 (MQ=255)                                                                       tttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                        >  2:234915/1‑151 (MQ=255)                                                                        ttCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCC‑TGGTCGCGCGGCAGTTTATAAATATTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                        <  1:5436/150‑1 (MQ=255)                                                                         tCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtctc                                                                      >  1:591883/1‑151 (MQ=255)                                                                          cACTTTCACCTCCAAGCGGGACGGTATTCCCCGCCCCC‑TGGGCGCGCGGCAGTTTATAAATATTCTTTGCGTGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                       <  2:51423/149‑1 (MQ=255)                                                                             tttCGACTTCAAGCGAGACGTTATTCCCCGGCCCC‑TGGCCGCGCGGCAGTTTATAAATATTCTGTTCATGAAGGAAAAAT‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                       <  1:173233/146‑1 (MQ=255)                                                                              ttCGACTTAAAGGGAGACGGTATTCCCCGCCCCC‑TGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                  <  1:697799/150‑1 (MQ=255)                                                                                 gACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCATGCATc                                                              >  2:1102719/1‑151 (MQ=255)                                                                                 gACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc                                                              >  2:796664/1‑151 (MQ=255)                                                                                 gACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc                                                              >  1:1000075/1‑151 (MQ=255)                                                                                   ctacAAGCGAGACGGTATTCCCCGGCCCC‑TGGCCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                             <  1:232925/147‑1 (MQ=255)                                                                                      cAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                         >  1:1140560/1‑151 (MQ=255)                                                                                      cAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                         >  1:112208/1‑151 (MQ=255)                                                                                       aggCGAGACGGTATTCCCCGGCCCC‑TGGTCGCGCGGCGGTTTATAAATATTCTGTTCATGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc                                                           <  1:476620/146‑1 (MQ=255)                                                                                           gagcctttatcccccGCCCCC‑TGGTCGCGCGACAGTTAATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                    <  2:446050/139‑1 (MQ=255)                                                                                           gagaCGTTATTCCCGGGCCCC‑TGGTCGCGCGGGGGTTTATGAATATTCTGTTCATGAAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                             <  1:446619/142‑1 (MQ=255)                                                                                            agaCGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                   >  2:922698/1‑151 (MQ=255)                                                                                                ggTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                               >  2:365912/1‑151 (MQ=255)                                                                                                 gttttccccGGCCCCTTGGCCGCGCGGCGGTTTATAATTTTTTTTTTAAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATg                                                     <  2:521045/139‑1 (MQ=255)                                                                                                  tATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCAAGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTGCcgcg                                             >  1:603510/1‑151 (MQ=255)                                                                                                    ttccccGGCCCC‑TGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                              <  1:6885/145‑1 (MQ=255)                                                                                                    ttccccGGCCCC‑TGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTc                                                  <  1:525290/141‑1 (MQ=255)                                                                                                       cccGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                        >  1:689532/1‑151 (MQ=255)                                                                                                       cccGCCCCC‑GGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                          <  1:782736/149‑1 (MQ=255)                                                                                                             ccc‑TGGCCGCGCGGCAGGTTATAAATTTTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGtt                                  <  2:1180683/151‑1 (MQ=255)                                                                                                                 tGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                >  2:646034/1‑150 (MQ=255)                                                                                                                 gggccgcgcgGCAGTTTATAAATTTTCTGTTCATGCAGAAAAACA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                               <  2:504912/147‑1 (MQ=255)                                                                                                                    tCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAgg                            >  1:70250/1‑151 (MQ=255)                                                                                                                       cgcgGCGGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                          <  1:370363/150‑1 (MQ=255)                                                                                                                       cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGCTTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                          >  2:622009/1‑150 (MQ=255)                                                                                                                          ggcggtttATGAATATTCTGTTCATGCGGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                        <  2:1110533/143‑1 (MQ=255)                                                                                                                          ggCAGGTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                        <  2:913612/149‑1 (MQ=255)                                                                                                                              ggTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                   >  1:407510/1‑150 (MQ=255)                                                                                                                                ttATGAATATTCTTTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                  <  2:267470/149‑1 (MQ=255)                                                                                                                                ttAGGAATATTCTTTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTACCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                  <  1:1015068/149‑1 (MQ=255)                                                                                                                                  aTGAATTTTCTTTTCGTCAAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg                <  2:1147696/149‑1 (MQ=255)                                                                                                                                    gAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc            >  1:108538/1‑151 (MQ=255)                                                                                                                                    gAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc            >  1:597172/1‑151 (MQ=255)                                                                                                                                     aaTATTCTGTTCAGGAGGGGAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc            <  2:1183551/150‑1 (MQ=255)                                                                                                                                        atTCTGTTCAGGGAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg         <  1:1163448/150‑1 (MQ=255)                                                                                                                                         tttttttCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc        <  1:1016860/145‑1 (MQ=255)                                                                                                                                            tGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg       >  1:194691/1‑148 (MQ=255)                                                                                                                                             gttaaggcggggAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc        <  1:951594/137‑1 (MQ=255)                                                                                                                                               tCATGCAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACt  <  1:67228/150‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                              ACCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACT  >  NC_000913/3556050‑3556340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑