Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,207	0	T	C	47.8%	74.2 / 26.5	67	S215G (AGT→GGT)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (30/5);  new base C (0/32);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 4.99e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCAC  >  NC_000913/3556059‑3556354                                                                                                                                                       |                                                                                                                                                    gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTc                                                                                                                                                    <  2:432321/150‑1 (MQ=255)   cGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTcccc                                                                                                                                                 <  1:286788/151‑1 (MQ=255)      aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGcc                                                                                                                                              >  2:240514/1‑151 (MQ=255)      aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGcc                                                                                                                                              >  2:5435/1‑151 (MQ=255)          accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtg                                                                                                                                          >  1:913634/1‑151 (MQ=255)            cTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtgt                                                                                                                                         >  2:217990/1‑150 (MQ=255)              cGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAg                                                                                                                                       >  2:247788/1‑150 (MQ=255)                          gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                                          >  2:550507/1‑151 (MQ=255)                                 aCCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAAc                                                                                                                    >  1:1128799/1‑150 (MQ=255)                                     acgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc                                                                                                               >  2:66718/1‑151 (MQ=255)                                        aaaaaaaaGGGTTCGGTGATTTTTTCTCTTACGCCTAAAGGCGAGCCGTTATTCCCCGGCCCC‑TGGCCGCGGGGCAGTTTATAATTATTTTGTTCAGGAGGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc                                                                                                               <  2:662053/146‑1 (MQ=255)                                          aaaaaaGCGTTCGGTATTTTTTTCCCTTCCGACTCAAAGGGAGACGTTTTTCCCCGCCCCC‑TGGCCGCGCGGCAGTTTATAAATTTTCTGTTCAGGAAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                                <  2:437434/145‑1 (MQ=255)                                               aGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                                     >  1:1139970/1‑151 (MQ=255)                                                  gtgcGGTAATATTTTCACTTTCAACTTAAGGGGAGACGTTATCCCCCGGCCCC‑GGGGCGCGGGGCGGTTTAAGAATATTCTTTACATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                                    <  1:337663/146‑1 (MQ=255)                                                     gggTGATATTTTCACTTCCCACTTAAGGGGGGACGTTATCCCCCGCCCCC‑TGGTCGCGCGGCGGTTTAGAAATTTTCTGTCCA‑GGAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                                 <  2:436567/148‑1 (MQ=255)                                                      gttatttttttCACTTCCAATTCAAAGCGAG‑CGGTTTTCCCCCGCCCCTTGCCCGCGCGACTGTTTATAATTTTTTTGTTCATG‑AGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                                 <  2:849436/141‑1 (MQ=255)                                                       gttttatTTCCACTTCCGACTCCAAGCGGGCCGTTATCCCCCGCCCCC‑GGGCCGCGCGGCGGTTTATAAATTTTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAg                                                                                                   <  2:651873/141‑1 (MQ=255)                                                        tGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                              >  1:893126/1‑149 (MQ=255)                                                           ttttttCCTTTCCGTCTACAACCGAGACGTTATCCCCCGGCCCC‑TGGCCGCGCGGCAGTTTATGATTATTCTGTTCGTGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                           <  2:369232/147‑1 (MQ=255)                                                             ttttCCCTTTCGCCTCAAAGCGAGACGTTATCCCCCGCCCCC‑TGGTCGCGCGGAAGGTTATAATTTTTCTTTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                                        <  1:837064/150‑1 (MQ=255)                                                              tttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                                      >  2:234915/1‑151 (MQ=255)                                                               ttCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGCCCCC‑TGGTCGCGCGGCAGTTTATAAATATTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                                      <  1:5436/150‑1 (MQ=255)                                                                tCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtctc                                                                                    >  1:591883/1‑151 (MQ=255)                                                                 cACTTTCACCTCCAAGCGGGACGGTATTCCCCGCCCCC‑TGGGCGCGCGGCAGTTTATAAATATTCTTTGCGTGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                                     <  2:51423/149‑1 (MQ=255)                                                                    tttCGACTTCAAGCGAGACGTTATTCCCCGGCCCC‑TGGCCGCGCGGCAGTTTATAAATATTCTGTTCATGAAGGAAAAAT‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtct                                                                                     <  1:173233/146‑1 (MQ=255)                                                                     ttCGACTTAAAGGGAGACGGTATTCCCCGCCCCC‑TGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                                <  1:697799/150‑1 (MQ=255)                                                                        gACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCATGCATc                                                                            >  2:1102719/1‑151 (MQ=255)                                                                        gACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc                                                                            >  1:1000075/1‑151 (MQ=255)                                                                        gACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc                                                                            >  2:796664/1‑151 (MQ=255)                                                                          ctacAAGCGAGACGGTATTCCCCGGCCCC‑TGGCCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                           <  1:232925/147‑1 (MQ=255)                                                                             cAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                                       >  1:112208/1‑151 (MQ=255)                                                                             cAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                                       >  1:1140560/1‑151 (MQ=255)                                                                              aggCGAGACGGTATTCCCCGGCCCC‑TGGTCGCGCGGCGGTTTATAAATATTCTGTTCATGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc                                                                         <  1:476620/146‑1 (MQ=255)                                                                                  gagcctttatcccccGCCCCC‑TGGTCGCGCGACAGTTAATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                                  <  2:446050/139‑1 (MQ=255)                                                                                  gagaCGTTATTCCCGGGCCCC‑TGGTCGCGCGGGGGTTTATGAATATTCTGTTCATGAAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                           <  1:446619/142‑1 (MQ=255)                                                                                   agaCGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                                 >  2:922698/1‑151 (MQ=255)                                                                                       ggTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                             >  2:365912/1‑151 (MQ=255)                                                                                        gttttccccGGCCCCTTGGCCGCGCGGCGGTTTATAATTTTTTTTTTAAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATg                                                                   <  2:521045/139‑1 (MQ=255)                                                                                         tATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCAAGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTGCcgcg                                                           >  1:603510/1‑151 (MQ=255)                                                                                           ttccccGGCCCC‑TGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                            <  1:6885/145‑1 (MQ=255)                                                                                           ttccccGGCCCC‑TGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTc                                                                <  1:525290/141‑1 (MQ=255)                                                                                              cccGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                                      >  1:689532/1‑151 (MQ=255)                                                                                              cccGCCCCC‑GGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                                        <  1:782736/149‑1 (MQ=255)                                                                                                    ccc‑TGGCCGCGCGGCAGGTTATAAATTTTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGtt                                                <  2:1180683/151‑1 (MQ=255)                                                                                                        tGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                              >  2:646034/1‑150 (MQ=255)                                                                                                        gggccgcgcgGCAGTTTATAAATTTTCTGTTCATGCAGAAAAACA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                             <  2:504912/147‑1 (MQ=255)                                                                                                           tCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAgg                                          >  1:70250/1‑151 (MQ=255)                                                                                                              cgcgGCGGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                                        <  1:370363/150‑1 (MQ=255)                                                                                                              cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGCTTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                                        >  2:622009/1‑150 (MQ=255)                                                                                                                 ggcggtttATGAATATTCTGTTCATGCGGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                                      <  2:1110533/143‑1 (MQ=255)                                                                                                                 ggCAGGTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                                      <  2:913612/149‑1 (MQ=255)                                                                                                                     ggTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                                 >  1:407510/1‑150 (MQ=255)                                                                                                                       ttATGAATATTCTTTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                                <  2:267470/149‑1 (MQ=255)                                                                                                                       ttAGGAATATTCTTTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTACCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                                <  1:1015068/149‑1 (MQ=255)                                                                                                                         aTGAATTTTCTTTTCGTCAAGGAAAAAA‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg                              <  2:1147696/149‑1 (MQ=255)                                                                                                                           gAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc                          >  1:597172/1‑151 (MQ=255)                                                                                                                           gAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc                          >  1:108538/1‑151 (MQ=255)                                                                                                                            aaTATTCTGTTCAGGAGGGGAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc                          <  2:1183551/150‑1 (MQ=255)                                                                                                                               atTCTGTTCAGGGAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg                       <  1:1163448/150‑1 (MQ=255)                                                                                                                                tttttttCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc                      <  1:1016860/145‑1 (MQ=255)                                                                                                                                   tGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg                     >  1:194691/1‑148 (MQ=255)                                                                                                                                    gttaaggcggggAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc                      <  1:951594/137‑1 (MQ=255)                                                                                                                                      tCATGCAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACt                <  1:67228/150‑1 (MQ=255)                                                                                                                                                aaaac‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGccac     <  2:387731/151‑1 (MQ=255)                                                                                                                                                aaaaa‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGccac     >  1:610958/1‑151 (MQ=255)                                                                                                                                                 aaac‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGccacc    <  2:750155/151‑1 (MQ=255)                                                                                                                                                    a‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCcaccac  >  2:451967/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                    GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCAC  >  NC_000913/3556059‑3556354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑