Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA43.6% 147.6 / 56.4 101A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (47/10);  new base A (0/44);  total (47/54)
Fisher's exact test for biased strand distribution p-value = 2.99e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTA  >  NC_000913/3665558‑3665849
                                                                                                                                                 |                                                                                                                                                   
caaCGAGTAATGAAGAAACTTTTTTATGGGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAc                                                                                                                                               >  2:532449/2‑151 (MQ=255)
  aCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAcc                                                                                                                                              >  1:608205/1‑150 (MQ=255)
         aTGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCAcc                                                                                                                                      >  2:964218/1‑151 (MQ=255)
           gaagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCg                                                                                                                                     <  1:488311/150‑1 (MQ=255)
                aaCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCAtt                                                                                                                                >  2:1190897/1‑150 (MQ=255)
                   ttttttATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAGTACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaa                                                                                                                              >  1:493088/1‑149 (MQ=255)
                      tttATGTGCATGAAGTTTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACACTTATCTACTCGATTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATa                                                                                                                          <  1:84420/150‑1 (MQ=255)
                        tATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCg                                                                                                                       >  1:1095898/1‑151 (MQ=255)
                        tATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCg                                                                                                                       >  2:315570/1‑151 (MQ=255)
                         aTGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCg                                                                                                                       >  2:17570/1‑150 (MQ=255)
                            tgCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGatat                                                                                                                   >  1:266647/1‑151 (MQ=255)
                              cATGAAGTTTTAATCCCTCCTCTTTGAGCATGGGGAAAAAAAAAGAAGGCGTGTCCTTTGACACAAATAAAAATTTTTCACCACTCTTATCTACTCAATTTTGCTTTGCATCCGAAAAAACCGGGTCTCCGCCATTAAAATAGCGATATTc                                                                                                                 <  1:814248/151‑1 (MQ=255)
                               ataaaGATTTAATCCCCCCTCCTTGAGCATGGGGAAAAAAAATAAAGGCGTTCCCTTTGACACAAATAAAAAATTTTCAACTCACTTATCTCCCCCAATTTGCTTCCCACCCGAAAAAACCAGTTCCCCGCCATTAAAATAGCGATAtt                                                                                                                  <  2:542315/146‑1 (MQ=255)
                                 gAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAcc                                                                                                              >  1:972569/1‑151 (MQ=255)
                                 gAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAcc                                                                                                              >  2:665419/1‑151 (MQ=255)
                                  aaGTTTTAATGCCTCCTCCTGGAGCATCGGGAAAAATAATAAAGGCGTGCCCCTTGACACAAATAAAAAATTTCAAACCCACTTATCTACTCAATTTTGGTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGata                                                                                                                    <  2:88528/144‑1 (MQ=255)
                                   aGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCACAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                            >  1:14622/1‑151 (MQ=255)
                                   aGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCAAATTTGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                            <  2:1033679/151‑1 (MQ=255)
                                     aTTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                           >  2:66202/1‑150 (MQ=255)
                                      tttAATCCCTCCTCCTTGACCATAGGGAAAAATAATAAAAGCGTGCCCCTTGACACAAATAAAAAATTTCCAACCCATTTATATCCACAAATTTGGCTTGCACCACAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                          <  2:283298/150‑1 (MQ=255)
                                      tttAACCCCCCCTCCTGGACCATGGGGAAAAATAATAAAAGCGTGTCCTTTGACACAAATAAAAAATTTTCAACACTCTTACCTCCTCGATTTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                          <  1:1101329/150‑1 (MQ=255)
                                       ttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                        >  1:878102/1‑151 (MQ=255)
                                        tAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                       >  2:239441/1‑151 (MQ=255)
                                         aaTGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                       >  2:641765/1‑150 (MQ=255)
                                         aaTGCCCCCCCTTTGAGCATCGGGAAAAATAATGAAAGCGTCCCCTTTGACACAAATAAAAAATTTTCACCCCCCTTATCTACCCAATTTTGGCTTGCCCCCGAAAAACCCAGCCCACCGCCATTAAAATAGCGATATTCACCATTAACCa                                                                                                      <  1:188219/151‑1 (MQ=255)
                                           tGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATg                                                                                                    >  1:678065/1‑151 (MQ=255)
                                             cccccccctGGAGCATCGGGAAAAATAATGAAAGCGTGCCCCTTAACACAAATAAAAAATTTTCAACACACTTATCTACTAGATTTTGGCTTGCATCCGCAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                                     <  2:1170730/142‑1 (MQ=255)
                                                cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                 >  1:286621/1‑149 (MQ=255)
                                                ccccctTGAGCATCGGGAAAAAAAATGAAGGCGTGTCCCTTGACACGAATAAAAAATTTCCAACACATTTATCTATCGTATTTTGGCTTGCACCCGCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                 <  1:715786/146‑1 (MQ=255)
                                                 ctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                 >  1:136276/1‑148 (MQ=255)
                                                 ctcctTGAGCATCGGGAAAAATAATAAAGGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACACTTATCTACTCAATTTGGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                <  2:801445/149‑1 (MQ=255)
                                                 cccctTGAGCGTCGGGAAAAATAATAAAGTCGTTCCCCTTGACACGAATAAAAAATTTACAACACTTTTATCACCTCAAATTTGGTTGCCATCAGCAAAACCAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                              <  2:573866/149‑1 (MQ=255)
                                                 cccctTGAGCATCGGGAAAAATAATGAAAGCGTGCCCTTTGACACAAAAAAAAAATTTCAAACACACTTATCTACTCAATTTTGGTTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgaga                                                                                              <  1:318445/149‑1 (MQ=255)
                                                    ctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                             >  1:1184595/1‑149 (MQ=255)
                                                    ctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                            >  1:1158112/1‑150 (MQ=255)
                                                      tGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatat                                                                                          >  2:180007/1‑150 (MQ=255)
                                                      tGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatat                                                                                          >  2:119113/1‑150 (MQ=255)
                                                        aGCATCGGGAAAAAAAATAAAGGCGTGTCCCTTGACACAAAAAAAAATTTTTCACCACACTTATCTACCCAATTTTGGCTTGCACCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                       <  1:25744/151‑1 (MQ=255)
                                                         gCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                      >  2:968474/1‑151 (MQ=255)
                                                           atgggaaaaaaTAATGAAAGCGTGTCCCTTGACACAAAAAAAAAATTTTCAACACATTTATCTACTAGAATTTGGCTTGCATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                                     <  2:604669/142‑1 (MQ=255)
                                                           aTCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                    >  2:532324/1‑151 (MQ=255)
                                                           aTCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                    >  2:6469/1‑151 (MQ=255)
                                                           aTCGGGAAAAATAATGAAAGCGTGCCCCTTGACACGAATAAAAAATTTTCAACACTCTTATCTCCTCGAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                        <  1:752238/147‑1 (MQ=255)
                                                              gggAGAAATAAGAAAATGGTTCCCTTTGACACAAATAAAAAATTTTCAACACACTTATTTACAAAATTTTGTTTTGCACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTc                                                                                 <  2:105642/151‑1 (MQ=255)
                                                              gggAAAAATAATAAAAGCGTGTCCCTTGAAACAAATAAAAATTTTCCAACACACTTATCTCCTCGATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                  <  1:741826/150‑1 (MQ=255)
                                                               gaaaaaaTAAGGAAAGTGGGTCCTTGGACAGAAAAACAAAATTTCCACCCCCCTTATCTACACGATTTTGGTTGGCACCCGAAAAACCTAGGTCACCGCCATTAAAGTAGCGATATTCACCATTAACCATGGTGAGAatat                                                                                          <  1:728352/137‑1 (MQ=255)
                                                                 agaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTg                                                                              >  1:136519/1‑151 (MQ=255)
                                                                  gaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGACTATATTTATGTCTTGc                                                                             >  2:354745/1‑151 (MQ=255)
                                                                  gaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGc                                                                             >  1:981600/1‑151 (MQ=255)
                                                                     ataatGAAAGCGTGTCCCTTGACACAAATACAAAATTTTCAACACACTTATCTACTCGATTTTGGCTTCCACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                          <  2:344311/151‑1 (MQ=255)
                                                                       aatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                        >  2:1055592/1‑151 (MQ=255)
                                                                       aatGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTCAACACATTTATCTCCTCGATTTTGGCTTGCACCCAAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                        <  2:689260/151‑1 (MQ=255)
                                                                       aatAAAAGGGTCCCCCTTGCCAAGAATAAAAAATTTACAACTTTCTTTTCTCCCAATATTTGGTTCGCCCCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                         <  1:477293/150‑1 (MQ=255)
                                                                       aagaaaaGCGTGTCCTTTGACACAAATAAAAAATTTTCAACACATTTATCTACTCAATTTTGGCTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                         <  1:779240/146‑1 (MQ=255)
                                                                           aaaGCGTGCCCCTTGACACAAATAAAAAATTTTCAACACACTTATCTCCTCAAATTTGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                    <  2:315941/151‑1 (MQ=255)
                                                                           aaaGCGTGCCCCTTGACACAAAAAAAAAATTTTCAACACACTTATCACCTCAATTTTGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                                      <  2:626444/149‑1 (MQ=255)
                                                                            aaGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAtt                                                                   >  2:398282/1‑151 (MQ=255)
                                                                              gCGTGTCCCTTGACACAAATAAAAAATTTTCAACACTCTTATCTACTCGAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAg                                                                 <  1:589864/151‑1 (MQ=255)
                                                                              gCGTGCCCCTGGACAAAAAAAAAAAATTTTCAACACCCTTATCTACTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAg                                                                 <  1:1137770/151‑1 (MQ=255)
                                                                                 tgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                               >  2:18643/1‑150 (MQ=255)
                                                                                   tCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                            >  2:132203/1‑151 (MQ=255)
                                                                                   tCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                            >  2:753813/1‑151 (MQ=255)
                                                                                    cccTTGACACAAATAAAAAATTTACAACCCATTTATCTACTCAATTTTGGCTTGCATCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                            <  2:242741/150‑1 (MQ=255)
                                                                                     ccTAGACACAAAAACAAAATTTACAACACACTTATCTCCTAAAATTTGGTTTGCATCCCAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                          <  2:968891/151‑1 (MQ=255)
                                                                                        tgacaaaaaaaaaaaaTTTAAAACACCCTTATCTACTAAAATTTGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                               <  2:509299/131‑1 (MQ=255)
                                                                                        tGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                        >  1:331267/1‑150 (MQ=255)
                                                                                          acacGAATACAAAATTTTCAACACACTTATCTACTCGATTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCAt                                                      <  1:797695/150‑1 (MQ=255)
                                                                                            acaaaTAAAAAATTTTCAACACATTTATCTTCGCAATTTTGTCTTGCACCCGCAAAAACAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                        <  2:443312/143‑1 (MQ=255)
                                                                                               aaTAAAAAATTTTAAACACACTTATCTACTAAAATTTGGTTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                                <  2:1149942/151‑1 (MQ=255)
                                                                                                  aaaaaaTTTTCAACACACTTATCTACTCGATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAt                                              <  1:1062464/148‑1 (MQ=255)
                                                                                                    aaaaTTTTAAACCCACTTCTTTACTCAAATTTGGCTGCCCCCCGCAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGt                                                 <  1:609667/145‑1 (MQ=255)
                                                                                                     aaaTTTTCAACACACTTATCTACTCAATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCa                                          <  1:708054/151‑1 (MQ=255)
                                                                                                      aaTTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                         >  1:611401/1‑151 (MQ=255)
                                                                                                      aaTTTTCAACACACTTATCTACTCAATTTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                         <  1:573754/151‑1 (MQ=255)
                                                                                                          ttCAACCCTCTTATCTACTCAATTTTGGCTTCCCTCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                      <  1:718071/150‑1 (MQ=255)
                                                                                                             aacccttttttCTACTCTATTTTGGTTTGCATCCGAAAAAACCGGGCAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                                    <  1:594349/139‑1 (MQ=255)
                                                                                                             aaCACACTTATCTCCTCGATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                                    <  1:964241/149‑1 (MQ=255)
                                                                                                                 cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                               >  1:754896/1‑150 (MQ=255)
                                                                                                                    ttATCTCCTCGAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                               <  1:6470/147‑1 (MQ=255)
                                                                                                                    ttATCTCCTCAAATTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAat                             <  1:829305/149‑1 (MQ=255)
                                                                                                                    ttATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                           >  2:585525/1‑151 (MQ=255)
                                                                                                                     tATCTACTCGAATTTGGTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                           <  1:1173329/150‑1 (MQ=255)
                                                                                                                      aTCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                          >  1:856076/1‑150 (MQ=255)
                                                                                                                       tctcCTCAAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                          <  1:264347/149‑1 (MQ=255)
                                                                                                                          aCTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                      >  2:1102627/1‑150 (MQ=255)
                                                                                                                          aCTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                      >  1:191173/1‑150 (MQ=255)
                                                                                                                          aCTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                      >  2:370100/1‑150 (MQ=255)
                                                                                                                           cTCGAATTTGGCTTGCACCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTa                         <  1:17571/146‑1 (MQ=255)
                                                                                                                            ccaaaaTTTGGTTTGCACCAGCAAAAACCATGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                      <  2:935633/145‑1 (MQ=255)
                                                                                                                                attttGGCTTGCACCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                  <  2:584315/147‑1 (MQ=255)
                                                                                                                                aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                >  1:690272/1‑151 (MQ=255)
                                                                                                                                aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat                >  1:706431/1‑151 (MQ=255)
                                                                                                                                  tttGGCTTACACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaaa                   <  1:821726/146‑1 (MQ=255)
                                                                                                                                  tttGCCTTCCATCCAAAAACTCCAGGCCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATa                     <  1:1060658/144‑1 (MQ=255)
                                                                                                                                     ggCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc           >  2:693306/1‑151 (MQ=255)
                                                                                                                                        ttGCATCCGAAAAACCCAGGTACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa            <  1:622486/147‑1 (MQ=255)
                                                                                                                                         tGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGtt       >  2:778782/1‑151 (MQ=255)
                                                                                                                                           cATCCAAAAAAACCAGGTCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGttt      <  1:1167222/150‑1 (MQ=255)
                                                                                                                                              ccGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttat    <  1:239444/149‑1 (MQ=255)
                                                                                                                                               cGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttatta  >  2:713333/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTA  >  NC_000913/3665558‑3665849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: