Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,381,728 | 0 | A | C | 46.7% | 77.8 / 14.2 | 60 | G197G (GGT→GGG) | frdA | anaerobic fumarate reductase catalytic and NAD/flavoprotein subunit |
Reads supporting (aligned to +/- strand): ref base A (22/10); new base C (0/28); total (22/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.26e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCG > NC_000913/4381580‑4381876 | cTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGAcc > 1:821004/1‑151 (MQ=255) tCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGAccc < 1:1007486/150‑1 (MQ=255) tCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGAcc < 2:360721/149‑1 (MQ=255) catcaGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCAcct < 2:300172/151‑2 (MQ=255) catcaGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCg > 2:707481/1‑151 (MQ=255) atcaGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGc > 2:1019081/1‑151 (MQ=255) ggATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGt > 2:1176196/1‑151 (MQ=255) ccgggCAGCCCGGTGGGGTGATACTGAACGAATTCCATGCCCCGCAGCGGACCGCCGTGGTTTAGCGCCATCCCCAACCCGTCCCCGGTAAC‑GAGGCCGCGGTTGGTGTTTTAACGAAAACCGCGACCCGCACCGCCAGTAGCCATAACGa < 2:116004/148‑1 (MQ=255) cTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGAc > 2:701137/1‑151 (MQ=255) gggaagccgggTTGGGTTATACTGAACGAATCCCATGTCACGCGGCGAAACGCGGTGGTTTACCCCCATCCCCATCCCTTCCCGGGTAAC‑GATGCCGCTGTTGGTGTTTTAACAATAACCGCGCCCCGCACCGCCAGTAGCCATAACGAcc < 1:664210/142‑1 (MQ=255) gCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACcgc > 2:1103070/1‑151 (MQ=255) gACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGtt > 1:752708/1‑151 (MQ=255) ggTTGGGTGATACTGAACGAATTCCATGCCCCGCAGCGGAACGCGGGGGCTTAGCCCCATCCCCATCCCGTCCCGGGAAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCa < 1:470734/151‑1 (MQ=255) tggggTGATACTGAACGATTCCCATGTCACGCAGCGGAACGCCGTGGTTTAGCGCCATCCCCACACCGTCACGGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGAAAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCAc < 1:243230/148‑1 (MQ=255) gATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTg > 1:591562/1‑151 (MQ=255) aTACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGc > 2:711047/1‑151 (MQ=255) tACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGc > 1:281801/1‑150 (MQ=255) tGACCGATTTCCATGTCACGAAGAGGAACGCCGTGGCTTGGCGCCATACCCATACCGTCACCGGAAAC‑GACCCCGCCGTTGGTGTTGTAAGGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCt < 1:1106109/145‑1 (MQ=255) accaattcccATGTCCCGCAGCGGAACGCCGTGGTTTAGCGCAATCCCCATCCCGTC‑CCGGGAACGGAGGCCGCCTTTGTTTTTGAAACGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGc < 2:1151409/143‑1 (MQ=255) aaCGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATCCCCATCCCGCCCCCGGAACC‑GATGCCGCCGTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAcc < 1:619417/148‑1 (MQ=255) aCGAATCCCATGTCCCGCGGCGGAACGCCGTGGCTTAGCGCCATCCCCATCCCGTCCCCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCg < 2:59079/151‑1 (MQ=255) gAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTg > 2:560156/1‑151 (MQ=255) aaaTCCCATGCCACGCAGCGGAACGCGGTGGTTTGGCGCCATCCCCATACCGCCACCGGAAAC‑GAGGCCGCCGTGGGTGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTg < 2:750986/150‑1 (MQ=255) aaTTCCATGTCCCGCAGCGGACCGCGGTGGCTTGGCGCCATCCCCATACCGCCACCGATAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCTCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGc < 1:221549/151‑1 (MQ=255) aaTCCCATGTAGCGAAGGGGAACGGGGGGTCTTAGCGCCACCCCCAGTCCGTCCCCGGAAAC‑GATCCGGCCGTGGGGGTTGAACCGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGt < 1:437396/149‑1 (MQ=255) ccATGTCACGCAGCGGACCGCCGTGGCTTAGCGCCATCCCCATCCCGTCCCGGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTc < 1:848255/151‑1 (MQ=255) caaTGTCACGAGGCGGACCGCCGTGGTTTAGCGCCATACCCATCCCGCCACCGGTAAC‑GATGCCGCGGTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCtt < 1:687796/148‑1 (MQ=255) cATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTcc > 1:774236/1‑151 (MQ=255) cgcGCAGCGGACCGCGGTGGCTTAGCCCAATCCCAAACCCGTCCCCGGAACC‑GATGCCGCCGTTGGTTTTGTAACAATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCcatcat < 2:703372/149‑1 (MQ=255) aCGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATg > 1:999935/1‑151 (MQ=255) cAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTc > 1:482524/1‑151 (MQ=255) gCGGAACGCCGTGGTTTGGCGCCATCCCCATACCGTCCCCGGTAAC‑GAGCCCGCCGTGGGTGTTGTAACAAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCa < 2:958287/150‑1 (MQ=255) aaCGCGGTGGCTTAGCCCCATCCCCATCCCTTCCCGGGTAAC‑GATCCCGCCTTTGTTGTTTTAACAATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCa < 1:532624/146‑1 (MQ=255) gCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTAcc > 2:1060515/1‑151 (MQ=255) ggggtttAGCGCCATCCCCATCCCGTCACCGGAAAC‑GATGCCGCCGTTGGTGTTGTAACGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAg < 1:1070411/145‑1 (MQ=255) gTGGCTAAGCGCAACACCCATTCCGTCACCGTAAAC‑GAGGCGGCGGTTGGTTTTGTAACGAAAACGGCCCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAgg < 2:996841/151‑1 (MQ=255) tGGTTTAGCGCCATCCCCACCCCGTCCCCGGAAAC‑GATCCCGCCGTTGGTTTTGTAACGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAg < 2:999913/149‑1 (MQ=255) ggCTTAGCGCCATCCCCATCCCGTCCCCGGTAAC‑GATGCCGCCGTTGGTGTTGAAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAg < 2:498979/148‑1 (MQ=255) ggCTTAGCCCCATCCCCATCCCGTCCCCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGc < 1:1033404/150‑1 (MQ=255) ggCTTAGCCCCACCCCAATCCCGTCCCCGTTACC‑GATGCCGCCGTTGTTGTTTTAACGAAAACCGGGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAg < 2:95041/148‑1 (MQ=255) cTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgc > 2:28197/1‑151 (MQ=255) ttggccccatccccatccCGCACCCGGTAAC‑GATGCCCCCGTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgc < 2:858194/144‑1 (MQ=255) tgacccccatccccatccCGCCCCCGGAAAC‑GATGCCGCCGTTGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcg < 2:734151/143‑1 (MQ=255) cgcCATCCCCATCCCGTCACCGGTAAC‑GATGCCGCCTTTGGTGTTGTAACGATAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAAc < 1:467842/150‑1 (MQ=255) cgcCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCTCTCATGTTCATTGCTACCAGGCCGCGAAc > 1:432794/1‑150 (MQ=255) cgcAATACCCACACCGCCCCCGGTAAC‑GTTCCCCCCGTGGGTTTTGTAACGAAAAACGCGCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCcgc < 2:974245/146‑1 (MQ=255) ccccgggTAAC‑GATGCCGCTTTTGGTGTTGTAACAAAAAACGCGCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCa < 1:892770/146‑1 (MQ=255) cgggaaaC‑GATGCCCCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAg < 1:780079/144‑1 (MQ=255) ggTACC‑GAGGCCGCCGTTGTTGTTGTAACGATAAACGCGACCCCCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAata < 2:820984/151‑1 (MQ=255) ggTAAC‑GATGCCGCCGTTGGTGTTTTAACAAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAata < 2:785869/151‑1 (MQ=255) ggaatcaaTGCCCCCGTTGGTGTTGAAACAAAAACCGCAACCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGa < 1:1176492/141‑1 (MQ=255) aaC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATc < 1:670163/150‑1 (MQ=255) cgTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATg > 2:274350/1‑151 (MQ=255) ggTGTTGTAACGATAAACGCGCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATg < 2:121709/147‑1 (MQ=255) ttgaAACGAAAACCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCa < 1:253428/150‑1 (MQ=255) aCGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGc > 1:229127/1‑151 (MQ=255) aaccgcgCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGAt < 2:414178/148‑1 (MQ=255) aaaCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGa > 2:551399/1‑150 (MQ=255) aaaCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGAt > 2:248230/1‑151 (MQ=255) cgACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCg > 1:971910/1‑151 (MQ=255) | CTTCGGTCATCAGGATACCGGAACCTGGCAGACCGGTTGGGTGATACTGAACGAATTCCATGTCACGCAGCGGAACGCCGTGGCTTAGCGCCATACCCATACCGTCACCGGTAAC‑GATGCCGCCGTTGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACATGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCG > NC_000913/4381580‑4381876 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |