Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	48.5%	98.8 / 16.4	68	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (8/27);  new base G (33/0);  total (41/27)
Fisher's exact test for biased strand distribution p-value = 3.62e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCG  >  NC_000913/354142‑354433                                                                                                                                                   |                                                                                                                                                  gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                               <  2:670404/151‑1 (MQ=255)      tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATg                                                                                                                                           >  2:980452/1‑150 (MQ=255)          tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGaag                                                                                                                                           >  2:269265/1‑144 (MQ=255)          tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATg                                                                                                                                           >  1:312742/1‑146 (MQ=255)           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTg                                                                                                                                      <  2:441542/150‑1 (MQ=255)              gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGaa                                                                                                                                    >  1:484397/1‑149 (MQ=255)                    ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAgg                                                                                                                            >  1:806788/1‑151 (MQ=255)                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCTGGAAGGGCgggggg                                                                                                                         >  2:1058066/1‑151 (MQ=255)                               tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                                  <  1:958608/150‑1 (MQ=255)                                  tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGtt                                                                                                               <  1:254454/150‑1 (MQ=255)                                   ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGGAAGATGCGCTGAAAGGGGAggtggcggggggggtt                                                                                                              >  1:253564/1‑145 (MQ=255)                                      gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGCGGTGGGGGGGGCGgtt                                                                                                              >  2:1122340/1‑147 (MQ=255)                                      gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCCGGAAAGGGAggggggggggggggtt                                                                                                              >  2:293654/1‑142 (MQ=255)                                      ggacgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCa                                                                                                          <  1:410799/148‑1 (MQ=255)                                           cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGTTTTTCATTcc                                                                                                      >  1:495641/1‑150 (MQ=255)                                             cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGGAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCg                                                                                                     >  2:754868/1‑149 (MQ=255)                                              tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAGGTGGCGGTGGTTGGGGGGAAAAAAGCGCCGGAAGGGGAGGGGGGGGGGGGGGTTggagatccgaa                                                                                                   >  2:803048/1‑140 (MQ=255)                                              tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCCGGGGGGGGGGGGGTTTGTCATTCCgga                                                                                                   >  1:130030/1‑148 (MQ=255)                                               gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                  <  2:160587/150‑1 (MQ=255)                                               gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                  <  1:652671/150‑1 (MQ=255)                                                aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAaga                                                                                                <  1:392668/151‑1 (MQ=255)                                                 cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGCGTTTGTCATTTCGaaa                                                                                                  >  1:1136129/1‑148 (MQ=255)                                                  gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagaga                                                                                              <  1:1053816/151‑1 (MQ=255)                                                       aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAAGGGAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCg                                                                                           >  1:828003/1‑149 (MQ=255)                                                         ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCg                                                                                           >  1:703580/1‑147 (MQ=255)                                                          tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCCGAAAGGGGAGGGGGCGGGGGGGGTTGTCATTTCGGAagagag                                                                                             >  2:654777/1‑144 (MQ=255)                                                            aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                    <  1:42126/151‑1 (MQ=255)                                                               ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTTCGAAAGAGAGGGACAGTCTgg                                                                                  >  1:344179/1‑150 (MQ=255)                                                               ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCGGAAGAGAGCGGCAGGCTg                                                                                   >  2:1788/1‑149 (MQ=255)                                                                 gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                               <  2:586962/151‑1 (MQ=255)                                                                 gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                               <  2:414789/151‑1 (MQ=255)                                                                   cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGTCTgg                                                                                  >  1:917230/1‑146 (MQ=255)                                                                          cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                                       <  1:845526/150‑1 (MQ=255)                                                                           ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGGGGGGGCGGTTGTCATTTCGAAAAAGAGCGACAGTCTGGGAGATCgtggtg                                                                      >  2:277468/1‑150 (MQ=255)                                                                           ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGTGGGGGGGGCGGTTTTCATTCCCAAAAAGAGGGACAGTCTGGGAGATCgtggtg                                                                      >  2:447718/1‑150 (MQ=255)                                                                            gCTGGGGACGCGCGAGATTGAAGAGCGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGGGGGGGGGGTTTTCATTCCCGAAGAGAGGGACAGTCTGGAAGATCGTGa                                                                        >  1:821235/1‑147 (MQ=255)                                                                              tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTgg                                                                   >  1:762917/1‑150 (MQ=255)                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGAAGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTTTCATTCCGAAAGAGAGGGACAGGCTGGGAGATCGTGATGTGgcg                                                                 >  2:585970/1‑151 (MQ=255)                                                                                   aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCCAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCAc                                                              >  1:86136/1‑150 (MQ=255)                                                                                    cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGGAAGAGAGGGACAGTCTGGAAGATCGTGATGTGGCGCACt                                                             >  2:178063/1‑150 (MQ=255)                                                                                     gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                           <  2:1167907/151‑1 (MQ=255)                                                                                          agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                       <  1:291718/150‑1 (MQ=255)                                                                                            aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgtga                                                    <  2:484383/151‑1 (MQ=255)                                                                                              tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGGAAAAGAGCGACAGGCTGGAAGATTGTGGTGTGGGGCAATCGCAagag                                                     >  2:1187642/1‑148 (MQ=255)                                                                                              tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGACGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                    >  1:542121/1‑149 (MQ=255)                                                                                               gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                 <  1:874235/151‑1 (MQ=255)                                                                                               gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                  <  2:1099215/150‑1 (MQ=255)                                                                                                 agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                               <  2:321760/151‑1 (MQ=255)                                                                                                 agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                               <  2:467318/151‑1 (MQ=255)                                                                                                    gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                            <  2:904260/151‑1 (MQ=255)                                                                                                      gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                          <  2:363256/151‑1 (MQ=255)                                                                                                           tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTTATTTCGAAAGAGAGCGACCGGCTGGAAGAACGTGGTGGGGCGGACTCGCAAGAGAAGGCGGTTATGgcg                                      >  1:16534/1‑150 (MQ=255)                                                                                                             cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                                   <  2:445259/151‑1 (MQ=255)                                                                                                                ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGAAAGATGCGCTGGAAGGGGAGGTGGCGGGGGCGGTTGTCCTTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGGTGGGGCGGACCCGCCAGAGAAGGGGGTTATGGCGCtgggg                                >  2:684096/1‑149 (MQ=255)                                                                                                                ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGGTTGTCATTTCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAGGGGATTATGGGGCtggtg                                >  2:151058/1‑151 (MQ=255)                                                                                                                ccATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCTGGAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGGGCACTCGCAAGAGAAGGGGGTTATGGCGCtggtg                                >  2:602297/1‑151 (MQ=255)                                                                                                                     cGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGTGGAc                             >  1:610707/1‑149 (MQ=255)                                                                                                                         cGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAGGGGGCGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCCAGAGAAGGCGATTATGGGGCTGGGGGACAGcc                         >  2:173849/1‑149 (MQ=255)                                                                                                                            tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                     <  1:796374/150‑1 (MQ=255)                                                                                                                                gAAGAGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGc                 >  2:1060886/1‑150 (MQ=255)                                                                                                                                 aaGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa               <  1:1072939/151‑1 (MQ=255)                                                                                                                                       cGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg         >  2:472198/1‑151 (MQ=255)                                                                                                                                        ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc        >  1:692671/1‑151 (MQ=255)                                                                                                                                         gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc       >  2:627656/1‑151 (MQ=255)                                                                                                                                          tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc        <  2:1034927/149‑1 (MQ=255)                                                                                                                                            gtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc    >  1:722419/1‑151 (MQ=255)                                                                                                                                              tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg  >  1:520078/1‑151 (MQ=255)                                                                                                                                               ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg  <  1:969054/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCG  >  NC_000913/354142‑354433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑