Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,400,417	A→T	I44F (ATC→TTC)	hfq →	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,417	0	A	T	100.0%	269.2 / NA	68	I44F (ATC→TTC)	hfq	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (45/23);  total (45/23)

CATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATAC  >  NC_000913/4400269‑4400562                                                                                                                                                     |                                                                                                                                                  cATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTc                                                                                                                                                 >  2:899175/1‑151 (MQ=255) cATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTc                                                                                                                                                 >  1:515428/1‑151 (MQ=255)    ataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTg                                                                                                                                              <  1:463947/151‑1 (MQ=255)      aaGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGtt                                                                                                                                            <  1:1062503/151‑1 (MQ=255)          caaaGAGTGAATGCCTATGTGGTAATCTTTCCATTATCCGTTCCTGAACGCACTGCGGCGGGAGCGTGTTTCAGGTTCTATTTACTTGGGGTATGGAATTTAGCTGCAAGGGCAAATCGAGTCTTTTGCTCAGTTCGTGTTTCTGTTGaa                                                                                                                                         >  2:403698/2‑150 (MQ=255)           aaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGaaa                                                                                                                                        >  1:377212/1‑150 (MQ=255)                gagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACg                                                                                                                                  <  2:1145170/151‑1 (MQ=255)                gagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACg                                                                                                                                  >  2:1190493/1‑151 (MQ=255)                 agaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACgg                                                                                                                                 >  1:421633/1‑151 (MQ=255)                        cTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGcc                                                                                                                           >  2:1041201/1‑150 (MQ=255)                          aaGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGa                                                                                                                        <  1:966026/151‑1 (MQ=255)                                    cTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                                                                                                               >  2:692554/1‑150 (MQ=255)                                      ttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                                                                                                                <  2:453827/147‑1 (MQ=255)                                       tACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                                                                                                            >  2:418486/1‑150 (MQ=255)                                        aCAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCa                                                                                                           <  1:1003466/150‑1 (MQ=255)                                          aaGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgc                                                                                                        <  2:1183793/151‑1 (MQ=255)                                             aTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAt                                                                                                     >  2:1094445/1‑151 (MQ=255)                                                cGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc                                                                                                  >  2:902058/1‑151 (MQ=255)                                                cGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGCTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc                                                                                                  >  2:776513/1‑151 (MQ=255)                                                 gTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCt                                                                                                 >  1:601092/1‑151 (MQ=255)                                                 gTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCt                                                                                                 >  1:662518/1‑151 (MQ=255)                                                    ccTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTAc                                                                                               <  2:660462/150‑1 (MQ=255)                                                       gAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgtt                                                                                           <  2:113633/151‑1 (MQ=255)                                                          cGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTc                                                                                        >  1:647571/1‑151 (MQ=255)                                                           gCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTcc                                                                                       >  2:691072/1‑151 (MQ=255)                                                           gCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTcc                                                                                       >  1:1027964/1‑151 (MQ=255)                                                            cACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTccc                                                                                      >  1:144204/1‑151 (MQ=255)                                                                  gTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCg                                                                                >  1:392273/1‑151 (MQ=255)                                                                  gTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCg                                                                                >  2:964207/1‑151 (MQ=255)                                                                       gAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCgg                                                                           <  2:594136/151‑1 (MQ=255)                                                                        aaCGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGTTTTCTACTGTTGTCCCGTCTCGCCCgg                                                                           >  2:1127362/1‑150 (MQ=255)                                                                        aaCGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGt                                                                          >  1:359968/1‑151 (MQ=255)                                                                         aCGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGtt                                                                         >  2:687103/1‑151 (MQ=255)                                                                           gtgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtc                                                                       <  1:596142/151‑1 (MQ=255)                                                                           gtgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtc                                                                       >  2:1130618/1‑151 (MQ=255)                                                                              ttCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtctc                                                                     >  2:1060975/1‑150 (MQ=255)                                                                                ccAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatc                                                                  >  1:623321/1‑151 (MQ=255)                                                                                 cAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatc                                                                  >  2:740800/1‑150 (MQ=255)                                                                                 cAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatc                                                                  >  2:112677/1‑150 (MQ=255)                                                                                   gTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtctcatctca                                                               <  1:766882/151‑1 (MQ=255)                                                                                   gTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcaca                                                               <  1:766887/151‑1 (MQ=255)                                                                                    tttCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcac                                                                >  1:255976/1‑149 (MQ=255)                                                                                    tttCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcac                                                                >  2:5058/1‑149 (MQ=255)                                                                                     ttCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAg                                                              <  1:355242/150‑1 (MQ=255)                                                                                         atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaaca                                                         <  1:461662/151‑1 (MQ=255)                                                                                         atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaaca                                                         <  2:421482/151‑1 (MQ=255)                                                                                           ttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaacaa                                                        <  1:53928/150‑1 (MQ=255)                                                                                             atttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGc                                                     <  2:101139/151‑1 (MQ=255)                                                                                              tttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGc                                                     <  1:421402/150‑1 (MQ=255)                                                                                                 ggTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCgg                                                  <  1:781348/150‑1 (MQ=255)                                                                                                  gTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTg                                                <  1:832112/151‑1 (MQ=255)                                                                                                   tGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTgg                                               >  1:820813/1‑151 (MQ=255)                                                                                                    gAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGc                                              >  1:908837/1‑151 (MQ=255)                                                                                                       tGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGt                                           >  1:83954/1‑151 (MQ=255)                                                                                                             tAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagc                                     >  2:103226/1‑151 (MQ=255)                                                                                                             tAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagc                                     >  2:217880/1‑151 (MQ=255)                                                                                                             tAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagc                                     >  1:1142706/1‑151 (MQ=255)                                                                                                              aaGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagc                                     >  1:883466/1‑150 (MQ=255)                                                                                                                  tGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTa                                 >  2:412132/1‑150 (MQ=255)                                                                                                                   gCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAAc                               >  2:988929/1‑151 (MQ=255)                                                                                                                    cAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACt                              <  1:970473/151‑1 (MQ=255)                                                                                                                     aaGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACt                              >  1:434256/1‑150 (MQ=255)                                                                                                                           aaaTCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcacc                        >  2:1108245/1‑148 (MQ=255)                                                                                                                                      tttGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagcagc             <  1:371348/150‑1 (MQ=255)                                                                                                                                       ttGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgc           >  2:276529/1‑151 (MQ=255)                                                                                                                                         gATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcgc         >  1:961005/1‑151 (MQ=255)                                                                                                                                                tCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATAc  >  2:172018/1‑151 (MQ=255)                                                                                                                                                tCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATAc  >  1:25548/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAATAC  >  NC_000913/4400269‑4400562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑