Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,574,0510GT44.0% 161.2 / 38.9 85V384V (GTC→GTApqqLputative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand):  ref base G (5/42);  new base T (37/0);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 1.83e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGC  >  NC_000913/1573907‑1574201
                                                                                                                                                |                                                                                                                                                          
tttCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTAcatc                                                                                                                                                       >  2:172757/1‑150 (MQ=255)
  tCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGAcatcat                                                                                                                                                     <  2:1257843/150‑1 (MQ=255)
  tCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGAcatcat                                                                                                                                                     <  1:858432/150‑1 (MQ=255)
     aaaGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTAcatca                                                                                                                                                      >  1:869194/1‑146 (MQ=255)
     aaaGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGAATTTAcaacattgtg                                                                                                                                                 >  2:1067481/1‑147 (MQ=255)
       aGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGtt                                                                                                                                             <  2:822131/151‑1 (MQ=255)
       aGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAAATTTTAAATTATCGAGtt                                                                                                                                             >  1:211120/1‑151 (MQ=255)
              atGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagc                                                                                                                                      <  1:206674/151‑1 (MQ=255)
               tGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagc                                                                                                                                      <  1:418307/150‑1 (MQ=255)
                gTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagcag                                                                                                                                    <  2:28217/151‑1 (MQ=255)
                gTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagca                                                                                                                                     <  1:554136/150‑1 (MQ=255)
                   tctTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAAATTTTACATCATCGAGGTCTTCAGCAGaa                                                                                                                                  >  2:989134/1‑150 (MQ=255)
                     ttCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGTGAGATTTTACATCATCGCGAGCTTTCGCAGaaa                                                                                                                                 >  2:764730/1‑149 (MQ=255)
                        cGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCa                                                                                                                            <  1:501552/151‑1 (MQ=255)
                         ggCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCAt                                                                                                                           <  2:13844/151‑1 (MQ=255)
                           cGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTCTTTTGCAGAAAAAACa                                                                                                                            >  2:1312004/1‑148 (MQ=255)
                            gACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTTTTTAGCAGaaaaaac                                                                                                                             >  1:58639/1‑144 (MQ=255)
                                  aaCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTAATCAACGAATTTTTTCAACAAAAAAACATTCTGAt                                                                                                                     >  2:1382309/1‑148 (MQ=255)
                                   aCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTATATTTTACATCATTCAGTTTTTCAGCAGGAAAAACaagttggt                                                                                                                     >  1:99857/1‑140 (MQ=255)
                                   aCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAAt                                                                                                                <  2:285947/151‑1 (MQ=255)
                                   aCGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAAt                                                                                                                <  1:764602/151‑1 (MQ=255)
                                    cGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATg                                                                                                              <  1:752699/151‑1 (MQ=255)
                                    cGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTGCATTATCCAGTTTTTCAGAAGAAAAAACAAGCTGGTCAAt                                                                                                                >  2:152459/1‑150 (MQ=255)
                                        gTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTg                                                                                                          <  1:691330/151‑1 (MQ=255)
                                        gTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTg                                                                                                          <  2:506914/151‑1 (MQ=255)
                                         tattatTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTAAATCATCGAGTTTTTTTGCAGAAAAAACATGCTGGTCAATgggt                                                                                                           >  2:230793/1‑147 (MQ=255)
                                               ttAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAAct                                                                                                    <  1:762668/150‑1 (MQ=255)
                                                tAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCCAGGTTTTTTGCAGAAAAAACAAACTGATCAATGGGTTGCCa                                                                                                       >  2:81696/1‑147 (MQ=255)
                                                 aaTGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTTTTCAGCCGAAAAAACATGCTGATCAATGGGTGccca                                                                                                      >  2:688207/1‑144 (MQ=255)
                                                 aaTGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAGTTTTCATCATCGAGTTCTTCAGCAGAAAAAACATGCTGATCAATTGGTGCCCAatct                                                                                                  >  2:380477/1‑150 (MQ=255)
                                                       cTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCAACACGTTTTTCATCTGAAAAAACATGCTGATCAATCGTTGGCCACACTGCCAtt                                                                                            >  1:1417421/1‑150 (MQ=255)
                                                               cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGGTTTTTAGCAGAAAAACCAAGCTGGTTAAATGGTGCCAACTCTGCCCTTAATGCAtt                                                                                    >  1:531213/1‑150 (MQ=255)
                                                               cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGGTTTTCAGCAGAAAAAACATGCTGCTCAATGGTTTCCCACTCTGCCCTTAAAGCAtt                                                                                    >  1:1220546/1‑150 (MQ=255)
                                                               cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTc                                                                                   <  1:1003171/151‑1 (MQ=255)
                                                                aGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCg                                                                                  <  1:984023/151‑1 (MQ=255)
                                                                   cGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCGAGTTTTTTAGCAGAAAAAACAAGCTGATCAAAGGTTTCCCAATCTTCCCTTTATGCATTCgc                                                                                 >  1:902740/1‑149 (MQ=255)
                                                                   cGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag                                                                               <  2:303670/151‑1 (MQ=255)
                                                                   cGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag                                                                               <  1:423445/151‑1 (MQ=255)
                                                                     aCTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGGTCTTTAGCAGAAAAAACATGCTTGTCAAAGGTTGCCCACCCTGCCCTTAAAGCATTCccagc                                                                              >  1:1334524/1‑150 (MQ=255)
                                                                      cTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGGTCTTTAGCAGAAAAAACATGCTGGTTAATGGGTGCCCAATCTTCCATTAATGCAtt                                                                                    >  1:1169123/1‑143 (MQ=255)
                                                                       tGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGCTAAATTTCATATAATCAAATTCTTTTCTAGAAAAAACAAGCTGATCAAATGTTGCCAACCCTCCCATTAATGcgtattcccc                                                                              >  1:203813/1‑139 (MQ=255)
                                                                         gTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGCAGGTTTTGAATCATCCCGCTTTTCTGCCGAAAAACCACGCTGATCAAATGGTGCTAAATCTGCCATTTATTCATTTGTAGCATcc                                                                          >  2:306924/1‑150 (MQ=255)
                                                                           cAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTg                                                                        <  1:15899/150‑1 (MQ=255)
                                                                           cAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTAATCATCGAGGTTTTTCTCAGAAAAAACAATCTGGTTAATTGGTGGCAAATCCTCCATTTATGCATTCGCAGCATcc                                                                          >  2:472004/1‑148 (MQ=255)
                                                                             gCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTGAATCATCGGGGTTTTTTTCAGAAAAAACATGCTGATCAAATGTTGCCAAATCTTCCCTTAATTCAtt                                                                                    >  2:1002434/1‑136 (MQ=255)
                                                                                tACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTGCATCATCGAGTTCTTTAACAGAAAAAACATGCTGATCAATGGTTGCCAACTCTTCCATTAATGCATTCGCCGCATCCTTCAtatt                                                                  >  1:627947/1‑151 (MQ=255)
                                                                                  cGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCAtatt                                                                  <  2:345134/149‑1 (MQ=255)
                                                                                      aaTCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTCTTTTTCAGAAAAAACCAGCTGGTCAATGGTTGCCCACTCTGCCCTTAATGCATTTCCAGCATCCTGCCTATTATTGt                                                             >  2:1091214/1‑150 (MQ=255)
                                                                                         cACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGtctc                                                          <  2:1333843/150‑1 (MQ=255)
                                                                                           cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTCATCATCGAGTTCTTTTGCAGAAAAAACAATCCGATCAAAGGTTGCCAACTCTTCCCTTAATTCATTTGCCGCATCCTCCATATTATCTtctc                                                          >  2:317821/1‑148 (MQ=255)
                                                                                               cAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAt                                                    <  2:26190/150‑1 (MQ=255)
                                                                                                aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAttt                                                  <  1:570105/151‑1 (MQ=255)
                                                                                                aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTCATCATCCAGGTTTTTTCGCCGAAAAACACGCTGTTTAATTGTTTCCCACCCCTCCACTTAAGCATTCTCCGCATTCTCCCTATTTTCTTCTCCGGCAttt                                                  >  2:1383402/1‑151 (MQ=255)
                                                                                                 gCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGGGGTTTTTTGCCGAAAAAACATGCTGATCAATGGGTGCCCACTCTTCCATTTATGCATTTTCAGCATCCTGCATATTATCTTCTCGTGCAttt                                                  >  2:22154/1‑150 (MQ=255)
                                                                                                  cTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTGCATCAACGAGGTTTTTTACAGAAAAAACAAGCTGGTCAAATGGTTCCCACTCCTCCCTTTATTAATTCGCAGCAACCTGCATTATATTGTCTCggggaatt                                                  >  1:590134/1‑142 (MQ=255)
                                                                                                    tGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTaca                                               <  1:571960/150‑1 (MQ=255)
                                                                                                     gCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACg                                             <  1:1037106/151‑1 (MQ=255)
                                                                                                     gCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACg                                             <  1:501677/151‑1 (MQ=255)
                                                                                                     gCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTGCATCATCGAGTTTTTCAGCAGAAAAAACAAGCTGATCAATGGGTTGCCACTCTTCCCTTAATGCATTTGCAGCATCCTGCATATTTTCGTCTCGTGCAttt                                                  >  2:596328/1‑146 (MQ=255)
                                                                                                      cTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAAATTTTACATTAACCAGTTTTTCTCTAGAAAAACCATTCTGATCAATGGGTGTCAACCCTGCCCTTTATGCATTTGCAGGATCCTTAATATTATTGTCTCCTGCATTTTCACg                                             >  1:878640/1‑150 (MQ=255)
                                                                                                         aTCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTTCATCATCGAGGTTTTTAGCAGAAAAAACAAGCTGATCAAAGGTTGGCAACTCTTCCCTTTATACAATTGCAGCAACCCTCCTATTATTGTCTCCTGCATTTTCAAggga                                          >  1:701649/1‑148 (MQ=255)
                                                                                                          tCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGTTCTTTAGCAGAAAAAACATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGaaa                                         >  1:314623/1‑150 (MQ=255)
                                                                                                           cAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGaaaa                                        <  1:414318/150‑1 (MQ=255)
                                                                                                           cAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTAATCATCGGGTTCTTCAGCAGAAAAAACATGCTGATCAAAGGGTGGCCAATCTGCCATTTATGCATTTGCAGCATCCTGCATAATTTCTTTTCGTGCATTTTAACGGaaaa                                        >  1:1162290/1‑150 (MQ=255)
                                                                                                             aCCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGGTTTTTAGCAGAAAAAACATGCTGATCAAAGGGTGCCAACTCCGCCCTTTATTCATTCGCCGCATCCCGCCTATTATTGTCTCCTGCATTTTCACCGaaaaa                                       >  2:347974/1‑149 (MQ=255)
                                                                                                                gCATTTTTCAGCCAGGTGAGGCGGTTAAATTTTACAAAATCGAGGTCTTCAACAGAAAAAACCTGCTGATCAATAGTTGCCAACTCTTCCCTTAATGCATTCGCAGCATTCTGCCTATTTTCGTCTCCTGCATTTTCACGGAAAAACa                                     >  1:863129/1‑148 (MQ=255)
                                                                                                                 cATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGa                                 <  1:726733/151‑1 (MQ=255)
                                                                                                                 cATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGTTTTTTTGCCGAAAAAACATGCTGATCAATGGGTGCCCAATCTGCCATTAATGCATTCGCCGCATCCTGCCTATTATTGTTTCGTGCCTTTTAACGGgaaaa                                       >  2:767932/1‑145 (MQ=255)
                                                                                                                    ttttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAAAGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACt                               <  1:706886/150‑1 (MQ=255)
                                                                                                                     tttCAGCCAGGTGAGGCGGGGAGATTTTACATCATCCAGGTTTTTAGCCGAAAAAACCAGCTGATCAATGGTTGCCAACTCTTCCCTTAATGCATTTGCAGCATCCCTCATATTATTCTCTCCTGCATTTTTACGGgaaaaaa                                     >  1:426776/1‑141 (MQ=255)
                                                                                                                      ttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGa                             <  1:508366/150‑1 (MQ=255)
                                                                                                                        caggcaggTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATaa                          <  1:448036/151‑1 (MQ=255)
                                                                                                                         aGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAAt                         <  1:1297933/151‑1 (MQ=255)
                                                                                                                         aGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAAt                         <  1:172764/151‑1 (MQ=255)
                                                                                                                            cAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCgg                      <  1:8438/151‑1 (MQ=255)
                                                                                                                              ggTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGt                    <  1:750836/151‑1 (MQ=255)
                                                                                                                                  aGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCaa                <  1:891823/151‑1 (MQ=255)
                                                                                                                                   ggCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAAt               <  1:252897/151‑1 (MQ=255)
                                                                                                                                     cGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAAttt             <  2:58639/151‑1 (MQ=255)
                                                                                                                                      gggTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAAtttt            <  2:898788/151‑1 (MQ=255)
                                                                                                                                         tAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAAc         >  1:624958/1‑151 (MQ=255)
                                                                                                                                          aGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACg        <  2:371280/151‑1 (MQ=255)
                                                                                                                                          aGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACg        >  1:687430/1‑151 (MQ=255)
                                                                                                                                            aTTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCt      <  2:1304604/151‑1 (MQ=255)
                                                                                                                                               tGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcg   >  1:509516/1‑151 (MQ=255)
                                                                                                                                                gACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcgc  <  2:90996/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                          
TTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCGC  >  NC_000913/1573907‑1574201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: