Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC42.7% 169.4 / 12.4 89intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (45/6);  new base C (0/38);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 3.47e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCA  >  NC_000913/2001771‑2002067
                                                                                                                                                        |                                                                                                                                                      
cGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcc                                                                                                                                                     <  1:281105/150‑1 (MQ=255)
   tataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCAGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgc                                                                                                                                                 >  2:840460/1‑151 (MQ=255)
     taGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgccg                                                                                                                                               >  1:260950/1‑151 (MQ=255)
     taGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgccg                                                                                                                                               >  2:1024409/1‑151 (MQ=255)
     taGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgccg                                                                                                                                               >  1:924979/1‑151 (MQ=255)
       gagTGAATTCACGATAAACAGCCCTGCGTTATAGGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCccgccg                                                                                                                                               <  2:433974/149‑1 (MQ=255)
        agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATACGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTg                                                                                                                                            >  1:1257283/1‑151 (MQ=255)
              ttCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCgggg                                                                                                                                      >  2:477262/1‑151 (MQ=255)
                cacaaaaaccAGCCTGGCGTTATATGAGTTATGGGCATGATTTTCCGTTTCTGCGGGGTTTTTAATCGGACGTTTGGTGGGTGAAATGGGGGGTTTTTTGGGGGTTACAGGTAAATCCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGaa                                                                                                                                    <  2:568281/142‑1 (MQ=255)
                 aCGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGa                                                                                                                                     >  1:513374/1‑149 (MQ=255)
                    aaaaaCGGCCCTGCGTTATATGATTTATCGGCATGATTATCCTTTTCTGCGGGGTTTTTAATCGGACGATTAGGGGGTGAAATGGGGGGTTATTGGGGGGTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCAc                                                                                                                                <  1:602848/149‑1 (MQ=255)
                    aTAACCACCCCTGGTTTATATGAGTTATCGGTATAATTATCCGTTTCCGATGGGTTTTAAATCGGATATTTGGTGGGTGAAAAGAGGGGTTTTTGGGGGTTTCAAGGTAAATCCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAg                                                                                                                                   <  1:291236/148‑1 (MQ=255)
                      aaaCAGCCCGGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCGGGGTTTTTAATCGGAGGATTAGGGGGTAAAAGGGGGGGTTATTGGGGGGTTAAAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACg                                                                                                                               <  2:378106/150‑1 (MQ=255)
                      aaaCACCCCTGCGTTATATGATTTATCGCATTTATTATCTGTTTCTGCAGGGTTTTAATTCGGACGATAAGTGGGTAAAAGGGGGGGTTATTGGGGGTTTAAAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCa                                                                                                                                 <  2:850674/148‑1 (MQ=255)
                          aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCTGGTGGCGGGGAAGCACGTTGCt                                                                                                                          >  2:1364336/1‑151 (MQ=255)
                                gctttATATGATTTATCGAAATAATTATCCTTTTCGGCAGGTTTTTTAATCGGACGTTTAGTGGGTAAAAGGGGGGTTTATTGGGGGGTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa                                                                                                                      <  1:1142034/146‑1 (MQ=255)
                                  gTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                  >  1:596634/1‑151 (MQ=255)
                                   ttATATGAGTTATCGGCATGTTTTTCCTTTTCTGCAGGTTTTTTAATCGGAGGATTAGTGGGGAAAATGAGGGGTTATTTGGGGGTTACAGGAAAATTCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                                    <  1:1113609/148‑1 (MQ=255)
                                    tataTGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgc                                                                                                                >  2:341337/1‑151 (MQ=255)
                                      tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                               >  1:1024081/1‑150 (MQ=255)
                                         gAGTTATCGGCATGATTATCCGTTTCTGCGGGGTTTTTAATCGGACGATTAGGGGGTAAAATGGGGGTTTTTTGGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcgc                                                                                                              <  1:884256/148‑1 (MQ=255)
                                         gAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                           >  2:134138/1‑151 (MQ=255)
                                           gTTATCGGCATGATTATCCGTTTCTGCGGGGTTTTTAATCGGACGATTAGGGGGTGAAAGGGGGGTTTATTGGGGGGTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                           <  2:375418/149‑1 (MQ=255)
                                            ttttCGGAATGATTATCCTTTTCTGCGGGGTTTTTAAGCGAACAATTAGGGGGGAAAATGGGGGTTTATTGGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                           <  1:1354048/145‑1 (MQ=255)
                                            ttATCGGAATGATTATCCTTTTCGGCGGGGTTTTAAATCGGACGATTGGTGGGTAAAATGGGGGTTTATTGGGGGGTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa                                                                                                          <  1:792538/149‑1 (MQ=255)
                                             tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                       >  1:181863/1‑151 (MQ=255)
                                               tCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTg                                                                                                     >  1:1190337/1‑151 (MQ=255)
                                                  gCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg                                                                                                  >  1:855354/1‑151 (MQ=255)
                                                    aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                                 >  1:1150227/1‑150 (MQ=255)
                                                       aTTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                              >  2:991335/1‑150 (MQ=255)
                                                        ttttCCGTTTCTGCGGGTTTTTTAATCGGACGTTTGGTGGGTAAAAGGGGGGTTTTTTTGGGGGTTACAGTTAATTTCCAGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                             <  2:931114/147‑1 (MQ=255)
                                                        ttATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                            >  2:582703/1‑151 (MQ=255)
                                                          aTCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg                                                                                           >  2:814957/1‑150 (MQ=255)
                                                          aTCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg                                                                                           >  2:1282828/1‑150 (MQ=255)
                                                            ccGTTTCTGCGGGTTTTTTAATCGGACGATTGGTGGGTGAAATGAGGGTTTATTTGGGGGTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                         <  2:569233/150‑1 (MQ=255)
                                                            ccGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                        >  1:1278609/1‑151 (MQ=255)
                                                               tttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                                      >  1:420484/1‑150 (MQ=255)
                                                                   tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGcc                                                                                 >  1:688273/1‑151 (MQ=255)
                                                                      aGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                               >  2:295058/1‑150 (MQ=255)
                                                                        ggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                            >  2:700017/1‑151 (MQ=255)
                                                                          tttttAAGGGGAAGATAAGGGGTTAAAAGGAGGTGTTATTGGGGGTTAACTGATAATTTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                               <  2:1063872/146‑1 (MQ=255)
                                                                           ttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                         >  2:780373/1‑151 (MQ=255)
                                                                            tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                        >  2:1379426/1‑151 (MQ=255)
                                                                                   ggaggataggTGGGTAAATTGAGGGTTTATGGGGGGGTTAAGGGTAAATCCAGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                   <  1:633409/140‑1 (MQ=25)
                                                                                   ggaggaTTGGGGGGTAAAATGGGGGGTTTTTGGGGGTTAAAAGTAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                  <  2:1403056/150‑1 (MQ=25)
                                                                                   ggACGATTGGGGGGTGAAATGGGGGTTTTTTGGGGGGTTACGGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                  <  2:1142738/150‑1 (MQ=37)
                                                                                   ggACGATTAGGGGGTGAAATGAGGGGTTTTTTGGGGGTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                  <  2:395826/150‑1 (MQ=255)
                                                                                    ggacgatggggggTGAAAATGGGGGTTTTTTGGGGGTTTAAAGTAAAATCCAGGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                   <  2:1180403/139‑1 (MQ=25)
                                                                                     aCGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                                >  2:815371/1‑150 (MQ=255)
                                                                                     aCGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                                >  2:1039451/1‑150 (MQ=255)
                                                                                       gATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                             >  2:166958/1‑151 (MQ=255)
                                                                                        aTTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                             >  1:1207380/1‑150 (MQ=255)
                                                                                         ttAGTGGGGGAAAGGGGGGGTTTTTGGGGGTTTACAGGAAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                            <  1:638426/150‑1 (MQ=25)
                                                                                         ttAGGGGGTAAAAGGGGGGTTTTTTGGGGGGTTACAGATAATTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                              <  2:39351/148‑1 (MQ=25)
                                                                                           aGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                          >  1:783240/1‑150 (MQ=255)
                                                                                            gggggTAAAATGGGGGTTTTTTGGGGGGTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                                           <  1:75998/146‑1 (MQ=37)
                                                                                             gggggaaaaaggggggTTTTTTGGGGGTTAGCAGGAAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                             <  1:1282856/134‑1 (MQ=25)
                                                                                             ggggaaaaaTGGGGGGTTATTGGGGGTTTACAGGAAAATTCGAGCGAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACAAAAAtgtg                                                           <  1:850950/141‑1 (MQ=37)
                                                                                              gggaaaaaTGAGGGTTTATTGGGGGGTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaaa                                                       <  1:436352/145‑1 (MQ=38)
                                                                                               ggTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                                     >  2:1207073/1‑151 (MQ=255)
                                                                                               ggTGAAAAGGGGGGTTTTTTGGGGGTTTAAAGTAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                                          <  2:662362/147‑1 (MQ=25)
                                                                                                 tGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                    >  1:483701/1‑150 (MQ=255)
                                                                                                    aaaggggggGTATTTTGGGGTTAACAGTAAAATCCAAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                                 <  2:138356/146‑1 (MQ=25)
                                                                                                       gAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                             >  2:362132/1‑151 (MQ=255)
                                                                                                         gggtTTTTTTGGGGTTAACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                                           <  2:924958/151‑1 (MQ=25)
                                                                                                         ggggTTATTTGGGGGTTACAGGTAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                            <  2:762434/150‑1 (MQ=255)
                                                                                                          gggTTTTTTGGGGGTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                          <  1:352210/151‑1 (MQ=37)
                                                                                                           ggTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                                          >  2:924465/1‑150 (MQ=255)
                                                                                                             ttATTTGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                         <  1:796370/149‑1 (MQ=255)
                                                                                                              tttttGGGGGTTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                                        <  1:1238714/148‑1 (MQ=37)
                                                                                                              tATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                      >  2:954341/1‑151 (MQ=255)
                                                                                                                tttGGGGTTTACATGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                     <  1:1367581/150‑1 (MQ=255)
                                                                                                                ttggggggTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                     <  1:367280/147‑1 (MQ=255)
                                                                                                                ttggggggTTAAAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                     <  2:244217/147‑1 (MQ=37)
                                                                                                                       gTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                              >  1:370268/1‑150 (MQ=255)
                                                                                                                          tACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                           >  1:1117485/1‑151 (MQ=255)
                                                                                                                          tACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                           >  2:143227/1‑151 (MQ=255)
                                                                                                                          tACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                            <  1:134144/150‑1 (MQ=255)
                                                                                                                                    taaatgccataaaaaaaaaacCCCCGCCGTTGTCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt                        <  2:1257255/134‑1 (MQ=25)
                                                                                                                                     aattcccAGCCAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                     <  2:1025827/145‑1 (MQ=25)
                                                                                                                                     aaaTTCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                     <  1:1176619/150‑1 (MQ=255)
                                                                                                                                     aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt                     <  1:1132009/150‑1 (MQ=255)
                                                                                                                                      aaatCCAGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                            <  2:260943/140‑1 (MQ=255)
                                                                                                                                      aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                    >  2:1395926/1‑150 (MQ=255)
                                                                                                                                      aaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATaa                   <  1:991936/151‑1 (MQ=255)
                                                                                                                                        ttCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagc                 >  1:978365/1‑151 (MQ=255)
                                                                                                                                          caaGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcg                <  1:1395011/148‑1 (MQ=255)
                                                                                                                                           cAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc               >  1:517350/1‑150 (MQ=255)
                                                                                                                                             gcaaaaaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca              <  1:840480/144‑1 (MQ=255)
                                                                                                                                               cAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcgc          >  2:393621/1‑151 (MQ=255)
                                                                                                                                               cAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcgc          >  1:66690/1‑151 (MQ=255)
                                                                                                                                                   aaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCa      <  1:970436/151‑1 (MQ=255)
                                                                                                                                                   aaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc       >  2:1189439/1‑150 (MQ=255)
                                                                                                                                                     aaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAgg    <  2:596621/151‑1 (MQ=255)
                                                                                                                                                     aaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAgg    >  1:624465/1‑151 (MQ=255)
                                                                                                                                                        aCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCa  >  1:867990/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
CGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGCA  >  NC_000913/2001771‑2002067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: