Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,804	0	G	A	40.8%	109.2 / 31.1	71	P125S (CCA→TCA)	emrY	putative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (36/6);  new base A (0/29);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 2.37e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCT  >  NC_000913/2481654‑2481954                                                                                                                                                         |                                                                                                                                                        atatCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTgg                                                                                                                                                         >  2:163810/1‑151 (MQ=255)   atCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGaa                                                                                                                                                       <  2:1171454/151‑1 (MQ=255)           ccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑Gc                                                                                                                                              <  2:319896/151‑1 (MQ=255)             aGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑Gccc                                                                                                                                            >  1:941786/1‑151 (MQ=255)             aGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑Gccc                                                                                                                                            >  2:832703/1‑151 (MQ=255)             aGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑Gccc                                                                                                                                            >  1:136932/1‑151 (MQ=255)               cTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCg                                                                                                                                          >  1:988845/1‑151 (MQ=255)                 aaaGTTATCACAAATATACCCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAC‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGcc                                                                                                                                        <  1:636959/151‑1 (MQ=255)                  aaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGcc                                                                                                                                        >  2:1255657/1‑150 (MQ=255)                  aaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCa                                                                                                                                       <  1:1214219/151‑1 (MQ=255)                     ttATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCAtt                                                                                                                                     >  2:856316/1‑150 (MQ=255)                              atatAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGa                                                                                                                            >  1:13545/1‑150 (MQ=255)                               tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAAc                                                                                                                          >  1:207956/1‑151 (MQ=255)                               tataACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAAc                                                                                                                          >  2:801564/1‑151 (MQ=255)                                              tCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAatat                                                                                                           >  1:983567/1‑151 (MQ=255)                                                ggCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATc                                                                                                          >  1:549545/1‑150 (MQ=255)                                                  ccaaaatatGGGAGCAATAACCAGGTTAATTACCAATAATGCAAAACAAATTTTTTTTTTTTCGGGGGGATATTTCTTAAAAAAAAAC‑CTCT‑GTGAAAGAGAAATAACCG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAat                                                                                                             <  2:824702/140‑1 (MQ=255)                                                   ccACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGc                                                                                                       >  1:1268893/1‑150 (MQ=255)                                                      caTATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACAt                                                                                                   >  1:276499/1‑151 (MQ=255)                                                       atatGGGAGAGATAATCCCGGTCATTGACAATAATGCAAGAGAATATGTTTTTTTTTCGGGGGAATAATTCTTAAAAAATAAC‑CTCT‑GTGAAAGAAAAATCAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGcac                                                                                                     <  2:413114/148‑1 (MQ=255)                                                        tatCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATc                                                                                                  >  1:304552/1‑150 (MQ=255)                                                         atCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCa                                                                                                 >  1:1297456/1‑150 (MQ=255)                                                             gAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAGTAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCACCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGAACATCCAGAtt                                                                                            >  2:80605/1‑151 (MQ=255)                                                               gCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTgg                                                                                          >  1:331004/1‑151 (MQ=255)                                                               gCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTgg                                                                                          >  1:1197640/1‑151 (MQ=255)                                                                  aTAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCg                                                                                       >  2:218722/1‑151 (MQ=255)                                                                    aaTCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGCCTCTAAAAAATATCAGCACATCAAGATTGGTCGa                                                                                      >  2:788428/1‑150 (MQ=255)                                                                     accgcGGTTATTAACCATAAGCCAAGAACAATTTTTTTTTTTTCGGGCGAATAATCCCTAAAAAAAAAA‑CTCT‑GAGACGGTGAAATTAGCG‑CCACCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATa                                                                                    <  1:479767/147‑1 (MQ=255)                                                                        aCGGTCATTGACCAAAATGCCAGAGCAATTTTTTTTTTTTCGGGGGGATAATTCTTAAATAAAAAA‑CTCT‑GTGAAAGTGAAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATa                                                                                    <  1:879253/148‑1 (MQ=255)                                                                               ttGACCATAATGCAAGACAAATTTTTTTTTTTTCGGGCGGATAATT‑CTTAAAAAAAAA‑CCCTGGTAAAAGTGAAATTACCG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTaca                                                                             <  1:575067/148‑1 (MQ=255)                                                                                tcaCCAAAATCCAAGAGAAATTTTTTTTTTTTCTGGGGAATATTTCCTAAATAAAAAA‑CTCT‑GTAACGGTGAAATAAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAt                                                                          <  1:19823/148‑1 (MQ=255)                                                                                tGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAtt                                                                         >  1:392259/1‑151 (MQ=255)                                                                                 gACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAtt                                                                         >  1:362433/1‑150 (MQ=255)                                                                                  aCCATAATGCCAGAGAAAATGTTCTTTTTTCGGGCGGATATTTCCTAAAAAAAAAA‑CTCG‑GTGAAAGTGAAATAAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAAGCTaca                                                                             <  1:440596/145‑1 (MQ=255)                                                                                   ccATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTaa                                                                       <  1:150283/150‑1 (MQ=255)                                                                                   caaTAATCCAAGAGAAATTTTTTTTTTTCCTGGC‑GAAAATTTCTTAAAAAAAAACCTCT‑GTAAAAGTAAAATTACCG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAtt                                                                         <  2:867057/146‑1 (MQ=255)                                                                                    cATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATg                                                                     >  1:971270/1‑151 (MQ=255)                                                                                       aaTGCCAGAGAAATTTTTTTTTTTTCGGGCGAATAATTCTTTAATAAAAAC‑CTCT‑GGGACAGGGAAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAg                                                                  <  1:673635/151‑1 (MQ=255)                                                                                        aTGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GGGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGa                                                                    >  2:846629/1‑148 (MQ=255)                                                                                        aTGCCAAAGAAAATTTTTTTTTTTCGGGCGGATAATTCCTTAAAAAAAAA‑CTCT‑GAAACAGTGAAATAAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGa                                                                 <  2:1074476/151‑1 (MQ=255)                                                                                           ccAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGa                                                                 >  2:402161/1‑148 (MQ=255)                                                                                             agagaatttGTTTTTTTTTCTGGCGATTATTTCCTTAATAAAACC‑CTCT‑GTGAAAGTGAATTTACCG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACa                                                               <  2:1228098/140‑1 (MQ=255)                                                                                              gataaaaTGTTTTTTTTTCGGGCGTATATTCCATTAATAAGACA‑TTCT‑TTAAAGGTGAAATTACCG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGAc                                                                <  1:485389/142‑1 (MQ=255)                                                                                              gagCAAGTTTTCTTTTTTTTGGCGGATATGTTCTAAATAAAAAC‑CATA‑GAGCGAATGAAATCGCCG‑GCCCCGCCATTAACCCCTGAACGACTCGAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGc                                                             <  2:724644/149‑1 (MQ=255)                                                                                                  aattttttttttttCGGGGGGATATTTCATAAAAAAAAAC‑CTCT‑GTGAAAATAAAATAACCG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa                                                         <  2:760235/141‑1 (MQ=255)                                                                                                  aattttttttttttCGGGCGAATATTTCTTTAAAAAAAAC‑CTCT‑GTGAAGGGGAAATAAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaa                                                       <  1:263482/143‑1 (MQ=255)                                                                                                  aattttttttttttCGGGCGAATATTTCCAAAAAAAAAAA‑CTTT‑GAGAAAGGGAAATAAGCG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGa                                                           <  2:986516/139‑1 (MQ=255)                                                                                                  aacgttttttttttCGGGAGAATATTTCCTAAATAATAAA‑CTTT‑GGGCCATTGGAATAAAGG‑TCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaaa                                                       <  1:1087466/143‑1 (MQ=255)                                                                                                  aaaTTTTTTTTTTTCGGGCGAATAATTCTTTAAAAAAAAC‑CTCT‑GTGACAGTGAAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa                                                         <  1:584103/149‑1 (MQ=255)                                                                                                  aaaTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaaa                                                        >  2:1218445/1‑150 (MQ=255)                                                                                                       ttCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAg                                                   >  2:612126/1‑150 (MQ=255)                                                                                                             tttCTGGGGGATATTTCTTAAAAAAAAAC‑CTCG‑GTGACAGTGAAATAAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTg                                             <  1:232478/150‑1 (MQ=255)                                                                                                             tttCTGGCGAATAATTCCTAAAAAAAAAC‑CTCT‑GTGAAAGTGAAATAAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTg                                             <  2:1106564/150‑1 (MQ=255)                                                                                                                cTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaa                                           >  2:666503/1‑149 (MQ=255)                                                                                                                cTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGaa                                           >  2:219255/1‑149 (MQ=255)                                                                                                                            tCCTTAAAAAAAAACCTCG‑GTAACAGTGAAATAAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTaa                                      <  1:408290/143‑1 (MQ=255)                                                                                                                               ttAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTa                           >  1:120766/1‑150 (MQ=255)                                                                                                                               ttAAAAAAAAT‑CTCG‑GTGAAAGAGAAATTAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAaataat                               <  2:1006607/146‑1 (MQ=255)                                                                                                                                 aaaaataaA‑CTCT‑GGGACAGGGAAATTAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCtt                            <  1:1123531/144‑1 (MQ=255)                                                                                                                                   taataaA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                      >  2:212860/1‑151 (MQ=255)                                                                                                                                    aaaaaa‑CTCT‑GTGATAGTAAAATTAAGG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                      <  2:647456/147‑1 (MQ=255)                                                                                                                                    aaaaaa‑CTCT‑GGGACAGGGAAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                      <  1:626758/147‑1 (MQ=255)                                                                                                                                     aaaaacctct‑GTGAAAGTAAAATTACCG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCg                      <  2:1262446/144‑1 (MQ=255)                                                                                                                                       aaa‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCt                   >  1:1387272/1‑150 (MQ=255)                                                                                                                                       aaa‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCt                   >  1:503646/1‑150 (MQ=255)                                                                                                                                       aaa‑CTCT‑GTGACAGTAAAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTa                  <  1:1039477/151‑1 (MQ=255)                                                                                                                                         a‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAtt                >  2:843671/1‑151 (MQ=255)                                                                                                                                           ctct‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTc               >  1:1231101/1‑151 (MQ=255)                                                                                                                                              tctggaagagggAAATAACGCGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTAt                 <  2:535652/138‑1 (MQ=255)                                                                                                                                                gtgAGAGTGAATTTAGCG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCttt            <  1:913694/150‑1 (MQ=255)                                                                                                                                                gtgAAAGGGAAATTAACG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCt              <  2:13546/148‑1 (MQ=255)                                                                                                                                                   aaaGTGAAATTACCG‑CCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCttt            <  2:1197612/145‑1 (MQ=255)                                                                                                                                                       tGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGTCTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGCGACTGAAAGTACAAATAATCTTAATTCGCTTATTGTTTGTGCCa      >  2:476524/1‑149 (MQ=255)                                                                                                                                                         gAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCt  >  2:1024974/1‑151 (MQ=255)                                                                                                                                                         gAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCt  >  2:103685/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                        ATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAA‑CTCT‑GTGACAGTGGAATTAACG‑GCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCT  >  NC_000913/2481654‑2481954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑