Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,921,177	0	A	C	41.8%	93.6 / 12.3	55	G308G (GGT→GGG)	gudP	putative D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base A (26/6);  new base C (0/23);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 4.88e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACCG  >  NC_000913/2921033‑2921324                                                                                                                                                 |                                                                                                                                                    cATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCTCCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGc                                                                                                                                               <  1:739199/151‑1 (MQ=255)  aTAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGc                                                                                                                                               >  2:693491/1‑150 (MQ=255)      aGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAcc                                                                                                                                          >  2:186436/1‑151 (MQ=255)         cGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgcc                                                                                                                                       >  1:782160/1‑151 (MQ=255)          gatgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgccg                                                                                                                                      <  1:750190/151‑1 (MQ=255)           atgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGa                                                                                                                                     <  2:688203/151‑1 (MQ=255)               tcatcCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATaa                                                                                                                                  >  2:1342752/1‑150 (MQ=255)                   ccACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATaaaa                                                                                                                                <  1:448212/148‑1 (MQ=255)                   ccACTCAACGTTGACGTAGTGGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCCCCCAGCACACCGCCGATAaaacc                                                                                                                              <  1:665954/150‑1 (MQ=255)                    cACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgc                                                                                                                            >  2:998178/1‑151 (MQ=255)                     aCTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgc                                                                                                                            >  2:1186672/1‑150 (MQ=255)                     aCTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgc                                                                                                                            >  1:788379/1‑150 (MQ=255)                         aaCGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgcaaacc                                                                                                                       >  1:820267/1‑151 (MQ=255)                             ttGACGTAGTTGCAGAACCCCATCCCCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCACCCAGCACACCGCCGATAaaaccgcaaaccgc                                                                                                                     <  1:419635/149‑1 (MQ=255)                              tGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcgg                                                                                                                  >  1:332450/1‑151 (MQ=255)                                  gTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacg                                                                                                              >  1:1285183/1‑151 (MQ=255)                                  gTAGTTGCAGAACACCACCCCCACCGACAGAAACATGCCCATTACGATCGGTTTTTTACGCGAAATGTTCAGCGACCCCGGGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCcggaa                                                                                                                <  2:473134/149‑1 (MQ=255)                                  gTAGTTGAAGAACCCCACCCCCATCGACAGCAACATGCCCATTACGATGGGTGTTTTACGCGCAATGTCCAGCGACCCCGTGCGGCGCATCAGCCAGTCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacg                                                                                                              <  1:776559/151‑1 (MQ=255)                                     gTTGCAGACCCCCACCCCCACCGACAGCAACATCCCCATTACGATCGGTGTTTTACGCGAAATGTCCAGCGACCCCGTGCGGCGCATCAGCCAGCCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacggaa                                                                                                           <  1:227670/151‑1 (MQ=255)                                          aGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCca                                                                                                       >  2:1183818/1‑150 (MQ=255)                                                      ccatcGACAGCAACATCCCCATTCCGACCGGTTTTTTACGCGAATTGTCCAGCGACCCCGTGCGGCGCATCAGCCAGCCGAAAATACCCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGccc                                                                                               <  1:810042/146‑1 (MQ=255)                                                             cAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc                                                                                   >  1:787855/1‑151 (MQ=255)                                                                 acaatccccTTTCCGATGGGTGTTTTCCGCGCAATGTCCAGCGATCCCGGGCGGCCCACCAGCCAGCCGAAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACa                                                                               <  2:866431/145‑1 (MQ=255)                                                                 aaCATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGACCCCGGGCGGCCCATCAGCCAGTGGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc                                                                                   <  1:1235999/147‑1 (MQ=255)                                                                    aTGCCATTTCCGATGGGTTTTTTACGCGAAATGTCCAGCGACCCCGTGCGGCCCATCAGCCAGTCGAAAAAAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACAt                                                                              <  2:135330/149‑1 (MQ=255)                                                                      gCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcg                                                                          >  1:349627/1‑151 (MQ=255)                                                                       cccATACCGATCGGTGTTTTACGCGCAATGTCCAGCGACCCCGTGCGGCCCATCAGCCAGCGGAAAAAAACCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgc                                                                         <  2:1047466/151‑1 (MQ=255)                                                                        ccATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgcg                                                                        >  2:1110524/1‑151 (MQ=255)                                                                        ccATTACGATCGGTGTTTTACGCAAAATGTTCAGCGATCCCGGGCGGCGCATCAGCAAGTCGGAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgcg                                                                        <  1:214161/151‑1 (MQ=255)                                                                         cATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgcg                                                                        >  1:439620/1‑150 (MQ=255)                                                                             aCGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCt                                                                   >  2:494334/1‑151 (MQ=255)                                                                                 tCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAc                                                               >  2:217529/1‑151 (MQ=255)                                                                                       ttttACGCGAAATGTCCAGCGATCCCGTGCGGCGCATCACCCAGTCGAAAATAACCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAg                                                            <  1:779960/148‑1 (MQ=255)                                                                                        tttACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATa                                                         >  1:1012713/1‑150 (MQ=255)                                                                                              cgcAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCggg                                                  >  2:875963/1‑151 (MQ=255)                                                                                               acattcttCAGGCATCCCGCGCGGCGTCTCACCGAGTCGAAAATAACCCCACCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAAcc                                                     <  2:228382/141‑1 (MQ=255)                                                                                                 attttccAGCGACCCCGGGCGGCGCACCAGCCGGTCGAAAAAAACCCCCCCAACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAc                                               <  1:857413/145‑1 (MQ=255)                                                                                                 agtGTCCAGCAACCCCGTGCGGCGCACCGGCCAGTGGAAAAAAACCCCCCCCAACACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGa                                                 <  2:1328694/147‑1 (MQ=255)                                                                                                 aatttccAGCGACCCCGGGCGGCGCACCAGCCAGTCGAAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGa                                                 <  2:1367440/143‑1 (MQ=255)                                                                                                 aaTGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAc                                               >  2:161542/1‑151 (MQ=255)                                                                                                    gTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAcc                                              >  1:791232/1‑149 (MQ=255)                                                                                                     ttCAGCGATCCGGGGCGGCGCACCACCCAGGCGAAAATAACCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAg                                            <  1:251077/150‑1 (MQ=255)                                                                                                          cGACCCCGGGCGGCGCATCAGCCAGCCGGAAATAACCCCCCCAACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGta                                         <  2:981828/148‑1 (MQ=255)                                                                                                           gcccccGGGCGGCGCAGCAGCCGGTCGAAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAg                                            <  2:1285155/141‑1 (MQ=255)                                                                                                            aTCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaataa                                     >  2:378835/1‑150 (MQ=255)                                                                                                               ccGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaaga                                  >  2:452208/1‑150 (MQ=255)                                                                                                                 gTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagt                               >  1:1033047/1‑151 (MQ=255)                                                                                                                     ggcgCATCAGCCAGTCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaagaag                                <  1:1321847/146‑1 (MQ=255)                                                                                                                       cgcATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTc                          >  2:1377409/1‑150 (MQ=255)                                                                                                                               ccAGTGGGAAATAATCCCCCCCATCACACCGCCGAGAAAACCGCAAACCGCCGGAACGCAAGCCACAAAGCGAGCTGTCAGAAGCGACATCCCGCGTGCCTGCACCAGAGAAACCGGGAAACAGGTAATAAAGAAGGAAGGAAgggagggg                 <  1:809208/151‑6 (MQ=255)                                                                                                                               ccAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCg                    <  1:386354/148‑1 (MQ=255)                                                                                                                               ccAGTCGAAAATAACCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAgg                       <  1:1385191/145‑1 (MQ=255)                                                                                                                                     gaaaaTAATCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAg           <  1:998199/149‑1 (MQ=255)                                                                                                                                      aaaaTAACCCCCCCCAGCACGCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACa            <  1:699904/148‑1 (MQ=255)                                                                                                                                        aataatCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTAc        >  1:31914/1‑151 (MQ=255)                                                                                                                                              cccacccaGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACCg  >  2:105143/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACCG  >  NC_000913/2921033‑2921324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑