Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	43.0%	102.0 / 66.9	93	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (4/49);  new base T (40/0);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 4.09e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206285‑3206581                                                                                                                                                        |                                                                                                                                                   cAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTc                                                                                                                                                    <  2:333429/151‑1 (MQ=255)     gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                                <  2:853547/151‑1 (MQ=255)      tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGa                                                                                                                                                >  2:73277/1‑150 (MQ=255)         ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACaa                                                                                                                                             <  1:1093690/150‑1 (MQ=255)             cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                                                        <  2:489367/151‑1 (MQ=255)              aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaa                                                                                                                                       <  1:1371832/151‑1 (MQ=255)                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaaaa                                                                                                                                     <  2:265488/151‑1 (MQ=255)                 aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACaaaaaaaaaa                                                                                                                                     >  2:1120701/1‑150 (MQ=255)                   aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACt                                                                                                                                  <  1:1301955/151‑1 (MQ=255)                     tGCCATTACCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑GATTTTTTCTAACAaaaaaaaaaa                                                                                                                                    >  2:1354237/1‑147 (MQ=255)                      gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAACTgg                                                                                                                                >  1:1333528/1‑150 (MQ=255)                      gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                               <  2:679895/151‑1 (MQ=255)                       ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGAaaaaaaaaaaaa                                                                                                                                    >  2:293107/1‑145 (MQ=255)                       ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AATTTTTTCCTACAaaaaaaaaaa                                                                                                                                    >  2:860075/1‑145 (MQ=255)                        cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGt                                                                                                                             <  1:490003/151‑1 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCCAACAAAAAAAAAAAATGGAgg                                                                                                                             >  2:1346383/1‑149 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAaaaaaaaaaa                                                                                                                                    >  2:952673/1‑143 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAggt                                                                                                                            >  2:232338/1‑149 (MQ=255)                           tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAggt                                                                                                                            >  2:1272728/1‑147 (MQ=255)                             ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCTCATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCa                                                                                                                        <  1:1413419/151‑1 (MQ=255)                               tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCat                                                                                                                       >  1:835767/1‑150 (MQ=255)                                gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                                     <  1:703955/151‑1 (MQ=255)                                  tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCctg                                                                                                                      >  2:467956/1‑148 (MQ=255)                                   ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat                                                                                                                    <  1:707073/149‑1 (MQ=255)                                    tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTAATTTTTCaaaaaaaaaaaaaaaatcgag                                                                                                                               >  2:435651/1‑133 (MQ=255)                                    tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTTAAACAAATAAAAAAAATGGGGtt                                                                                                                            >  1:982488/1‑140 (MQ=255)                                     ggCGCAATATTCATTCTTATTTTCGCGTTGATTCATGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                                 <  2:994260/150‑1 (MQ=255)                                     ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                                 <  1:479263/150‑1 (MQ=255)                                      gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                               <  1:1195328/151‑1 (MQ=255)                                          gatatTCATTGTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑GATTTTTTCAGACAAAAAAAAAAAATGGGGTTTTCATGATGAGGGaaa                                                                                                            >  2:45359/2‑150 (MQ=255)                                           atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCCGAAAAATAAAAAAAATGGAGGTGGCctgatgcaggaaa                                                                                                            >  1:284894/1‑142 (MQ=255)                                           atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGt                                                                                                          <  2:1356641/151‑1 (MQ=255)                                             attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                         <  2:89306/150‑1 (MQ=255)                                                cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata                                                                                                      <  2:178787/150‑1 (MQ=255)                                                aattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGAG‑AATTTTTCCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGtaata                                                                                                      >  1:1320513/2‑150 (MQ=255)                                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGGGGAAGGAATAAGCa                                                                                                  >  1:708018/1‑150 (MQ=255)                                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAACTGGAGGTGCCATGATGAggggaaagaaaaaaca                                                                                                  >  1:1337356/1‑135 (MQ=255)                                                     ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAGGTGGCATGTTAAGCGAAAAtaataaaaaa                                                                                                 >  2:161551/1‑146 (MQ=255)                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGAAAAAAAAAAAAAAAGGAGGTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                 >  1:309708/1‑149 (MQ=255)                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCTTACAAATAAAAAAAAAGGAGTTGCCGTTATGAGCGAAAATAATAAACaa                                                                                                 >  1:1224034/1‑149 (MQ=255)                                                       aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                              <  2:1072472/151‑1 (MQ=255)                                                       aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTGTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                               <  1:1229087/150‑1 (MQ=255)                                                         tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAGGTTGCATGATTTGCGGAAGgaataaagaaaa                                                                                               >  2:640090/1‑142 (MQ=255)                                                          ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTTCCATGATGAGCGAAAGTAATAAACaaaa                                                                                               >  2:224165/1‑145 (MQ=255)                                                          ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATAGAGTTTGCCTGATGGGCGAAAGtaaaaaaaaaaa                                                                                               >  2:1375782/1‑141 (MQ=255)                                                             gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                        <  1:516563/151‑1 (MQ=255)                                                                     tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                                 <  1:108771/150‑1 (MQ=255)                                                                      cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATGGAGTTTCCATGATGAGGGAAAAGAAAAAGAAAAAAGCAGGGGATaa                                                                                   >  2:1187567/1‑147 (MQ=255)                                                                        cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAc                                                                             <  1:332058/151‑1 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                            <  2:880096/151‑1 (MQ=255)                                                                         gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                            <  2:433790/151‑1 (MQ=255)                                                                           aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga                                                                          <  1:574833/151‑1 (MQ=255)                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAACTGGAGGTGCCCTGATGAGCGGAAATAATAAACAAAAAGCAGTGGATAAGTTGaaa                                                                            >  1:737284/1‑145 (MQ=255)                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                    <  2:1300489/151‑1 (MQ=255)                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                    <  2:1321080/151‑1 (MQ=255)                                                                                  aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                   <  1:282124/151‑1 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                  <  1:869549/150‑1 (MQ=255)                                                                                      cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCAAAAAAAAAAAAAAAACGGAGTTGGCATTGTGAGCGGAAATAAAAAAAAAAAGGCAGGGAAAAAAATTACAGAGAtt                                                                      >  2:83704/1‑144 (MQ=255)                                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACCAAAAAAAAAAATGGAGTTGTCATTATTAGGGGAAGTAATAAACAACAAGCAGTGAATAAGATGACAGAGAATGTTGCCAACttt                                                          >  1:875584/1‑150 (MQ=255)                                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATAAAAAAACTGGAGTTGCCATTATGAGCGAAAGTAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACttt                                                          >  1:398666/1‑150 (MQ=255)                                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt                                                          <  2:110334/150‑1 (MQ=255)                                                                                            cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCCA‑ACAAATACAAAAAATGGGGTTTGCATTATGAGCGGAAGGAATAAGGAACAGGCAGAGAATAAGTTGACAGAGATTGTCGCTAACatt                                                          >  1:527543/1‑150 (MQ=255)                                                                                                    cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGAGCGGAAGTAAAAAACAAAAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTTACGcc                                                    >  1:249756/1‑148 (MQ=255)                                                                                                     aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTA‑AATTTTTCCAAACAAATACAAAAACGGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCGGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATg                                                 <  2:1276315/150‑1 (MQ=255)                                                                                                        aaaTATAAACCCACATTTTTAGTGGTAGTTTGTGGGGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAATAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                             <  2:1019377/151‑1 (MQ=255)                                                                                                           tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAAAAAAAAATGGGGTTGGCATGATGAGGGGAAGGAATAAACAACAGGGAGTGGATAAATTGACAGAGATTTTTGCCAAAATTTACGCCATGAttttt                                           >  1:584230/1‑148 (MQ=255)                                                                                                            ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAAAAAAAATAAAAAATGGGGTTGTCCTGATGAGCGGAAAGAATAAACAACAGGGAGAGGATAAATTGAAAGAGATTTGCGCTAAATTTAACCCCATGAtttt                                            >  2:18204/1‑147 (MQ=255)                                                                                                              aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGGAAGTAATAAGCAACAAGCAGGGAATAAGTTGACAGAGATTTTCGCTAACTTTACCCCCCTGATTTTTTccc                                       >  1:1300115/1‑151 (MQ=255)                                                                                                              aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAccc                                       <  1:952694/151‑1 (MQ=255)                                                                                                              aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAccc                                       <  1:1120724/151‑1 (MQ=255)                                                                                                              aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAcc                                        <  1:1197124/150‑1 (MQ=255)                                                                                                                aaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGGGAAAAAATTTACAGAGATTTTCGCTAACTTTAACCCCATGATTTCTAccc                                       >  1:1223518/1‑149 (MQ=255)                                                                                                                 aCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAGTTTCCATGATGAGGGAATGTAATAAGCAAAAGGCAGTGAAAAAGATTAAAGAGATTGTCGCTAACTTTACCCCCATGATTTTTAccc                                       >  1:1284002/1‑148 (MQ=255)                                                                                                                  ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                    <  1:936064/150‑1 (MQ=255)                                                                                                                  ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                     <  2:539440/149‑1 (MQ=255)                                                                                                                   cacaTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGGGGTGGCATGATGAGGGGAAGTAATAAACAAAAGGCAGGGAAAAAATTGACAGAGATTGTCGCTAACTTTACCCCCCTGATTTTTTCCCgca                                    >  2:1290430/1‑147 (MQ=255)                                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCACACAAAAAAAAAAACAGGAGGTGGCATGATGAGGGGAAGGAATAAAAAAAAAGCAGTGAATAAATTTACAGAGATTTTCGCTAAATTTATCGCCATGATTTTTTCCCCAAAGcc                                >  2:857475/1‑151 (MQ=255)                                                                                                                        ttttAGTGGTTGTTTGTGGTGGTA‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTAGACAGAGATTGTCGGTAATTTTGCCGCCATGATTTCTACCCGAATGCCtg                              <  2:1398617/150‑1 (MQ=255)                                                                                                                          ttAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCATGATGAGGGGAAATAATAAACAAAAAGCAATGAAAAAGATGACAGAGAATGTCGCTAACTTTATCCCCATGATTTTTACCCgcaaacccga                             >  2:1166026/1‑140 (MQ=255)                                                                                                                           tAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAATAATAAACAAAAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTTCCGCCCTGATTTTTACCCCAATGcc                                >  1:129523/1‑145 (MQ=255)                                                                                                                            aGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAAAAAAAAATGGGGTTGGCATGATGAGCGGAAGGAATAAACAACAAGCAGTGAAAAAATTTACAGAGAATTTTGCTAACTTTAACGCCCTGAtttttttcccaaagcc                                >  1:748884/1‑131 (MQ=255)                                                                                                                             gTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtga                             <  2:954616/146‑1 (MQ=255)                                                                                                                             gTGGTAGTTTGTGGCGGTA‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                         <  1:1026301/150‑1 (MQ=255)                                                                                                                              tGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg                        <  1:1303204/150‑1 (MQ=255)                                                                                                                                      tgtgGCGGTG‑AATTTTTTCCGGCAAAAACAAAAACAGGAGTTTGCATGATGAGGGGAAAGAAAAAACAAAAAGCAGGGAAAAAATTGACAGAGATTGTCGCTAAATTTAACCCCATGATTTTTATCCCAAAGCCTGGTGACGggggggg                 >  2:168870/1‑148 (MQ=255)                                                                                                                                      tgtgGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                <  1:798928/150‑1 (MQ=255)                                                                                                                                       gtgGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa              <  2:570264/151‑1 (MQ=255)                                                                                                                                            ggTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa         <  1:907728/151‑1 (MQ=255)                                                                                                                                            ggTG‑AATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa         <  2:1010856/151‑1 (MQ=255)                                                                                                                                            ggTG‑AATTTTACCAGCCAAATACAAAAACAGGAGTTGCCATAAAGCGAAAAAGTAAAAAACAACGGGCAGTAAATAAGTGGAGAGAGATGCTGGTTAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa             <  1:420434/147‑1 (MQ=255)                                                                                                                                                 aaTTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGTTGAGCAAAAGAAATAAACAACAGGCGGTGAATAAGTGAACGGATATTGTCTCTACCTTTACCGCCATGATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa     <  1:860096/151‑1 (MQ=255)                                                                                                                                                  aTTTTTCCAGACAAATACAAAAACTGGAGCTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      >  1:1014018/1‑149 (MQ=255)                                                                                                                                                   ttttcCCAGAAAAATACAAAATCGAGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGAAGTGAATAAGTTGACAGATATTCTCGCTAACTTTACCGCCATGATTTCTCCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg    <  2:604679/150‑1 (MQ=255)                                                                                                                                                     tttCAAGAAAAAAACAAAAACTGGAGTTGCCATAATGAGCAAAAGTAATAAGCAACAGGCATTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt  <  2:875563/150‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                   CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206285‑3206581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑