Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 366,526 | 0 | T | G | 42.1% | 140.1 / 22.1 | 76 | T329P (ACC→CCC) | lacI | lactose‑inducible lac operon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base T (8/36); new base G (32/0); total (40/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.41e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACA > NC_000913/366377‑366670 | aaaGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGggtg < 2:1089872/151‑1 (MQ=255) aGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGggtggt < 2:1246047/151‑1 (MQ=255) tGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGCGTA‑TTTGGCGCCCGGGTGGgtttt > 1:612471/1‑151 (MQ=255) tGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGGGGA‑TTTGGCGCCAGGGGGGGttttttttt > 2:576094/1‑151 (MQ=255) ccTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGATTTGGGGGCCGGGGGGGTtttttttt > 2:1024442/1‑149 (MQ=255) aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCa < 2:73167/151‑1 (MQ=255) aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCa < 2:825128/151‑1 (MQ=255) gtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTGGGGGCCCGGGGGGGTTTTCTTTTTTCCAGTg > 2:630983/1‑149 (MQ=255) tAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTTGGCGGCAGGGGGGGTTTTTTTTTCACCAGTGAGACggg > 1:404603/1‑150 (MQ=255) aCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGGGCCCGGGGGGGTTTTTTTTTTACCCGGGAGACggg > 1:95691/1‑148 (MQ=255) cacaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTGGGGGCCAGGGGGGGTTTTTTTTTTCCCCGTGAGACGGGGaa > 2:687769/1‑148 (MQ=255) cacaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAc < 2:1142593/149‑1 (MQ=255) cacaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACa < 1:1016093/150‑1 (MQ=255) cacaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAg < 1:639739/151‑1 (MQ=255) acaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGc < 2:689241/151‑1 (MQ=255) acaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGc < 1:1225264/151‑1 (MQ=255) aTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACa > 1:159250/1‑147 (MQ=255) ttAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTG‑TTTGGCGCCCGGGGGGTTTTTTTTTTTCCCAGTGAGACGGGGaa > 2:961618/1‑144 (MQ=255) tAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGa < 1:641255/150‑1 (MQ=255) aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAt < 1:1133466/150‑1 (MQ=255) tgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGAtttgc > 1:1421227/1‑148 (MQ=255) tgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGGAACAGCTGGTTg > 2:3718/1‑149 (MQ=255) tgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGc < 2:1334053/150‑1 (MQ=255) ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGGGGATTTGGGGGCCGGGGGGGTTTTT‑TTTTTACCCGGGAGACGGGGAACAGCTGATTGcccct > 2:327147/1‑148 (MQ=255) gcgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTTGTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTACCCGCGGGACGGGGGAAAGCTGATTGcccct > 2:833754/1‑146 (MQ=255) gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGTGCA‑TTTGGCGGCCGGGGGGTTTTTTTTTTTTCCCGAGAGAAGGGGAAAAGCTGGtt > 2:1277446/1‑140 (MQ=255) gcTCACTGCCCGCTTTCCAGTCGGAAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGTGTA‑TTTGGCGGCGGGGGGGGTTTTCTTTTTACCAGGGAGACGGGCAACAGCTGATTGCCCTTCAcc > 2:397599/1‑150 (MQ=255) tCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTTGGCGCCCGGGGGGGTTTTCTTTTTTCCACAGAGACGGGCCAAAGGTGATTGCCCTTCACCGc > 2:156630/1‑150 (MQ=255) cccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGccc < 1:714846/151‑1 (MQ=255) cccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGG‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGGGTGGCGGGGAACAGCTGATTTGCCCTCTCCGCCCGGgcc > 2:1005401/1‑151 (MQ=255) gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGGCCGGGGGGGTTTTT‑TTTTTACCAGGGGGACGGGGAACAACCGGTTGGCCGTCCCCCCCCGGGCCTgg > 2:1103138/1‑150 (MQ=255) gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGTGGA‑ATGGGCGTCACGGTGGTTTTTTTTTTTTCCAGGGAGAGGGGGGACAACTGATTTGCCCTCCCCACCCGGccc > 2:234233/1‑148 (MQ=255) tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTGGGCGCCCGGGGGGGTTTTTTTTTTTCCAGTGAGACGGGGAACAGCTGATTGCCCTTCACCGCCCGGCCCt > 1:1308145/1‑147 (MQ=255) tCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAAAAGCTGATTGCCCTTCACCGCCTGGCCCTGagagaggt > 1:261846/1‑147 (MQ=255) cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgca < 2:936579/151‑1 (MQ=255) gggAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTTCCAAGGAGACGGGGAACAGCTGATTTGCCTTCACCCCCCCGCCCTgggaggggt > 1:331349/1‑143 (MQ=255) gAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTGGGGGCCCGGGGGGTTTTTTTTTTTACCCGTGAGACGGGCAAAAGCTGATTGCCCTTCACCGCCCGGCCCCgagag > 1:109463/1‑141 (MQ=255) gAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGCGGG‑TTTGGCGCCCGGGGGGGTTTTTTTTTTACCAGTGAGAAGGGGAAAAACTGATTTGCCTTCACCCCCCGGCCCCgcgaggggt > 2:1273346/1‑141 (MQ=255) aCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGc < 1:1103161/151‑1 (MQ=255) ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGGAACAGCTGATTGCCCTTCACCCCCTGGGCCTGGGGGGGGTGCAGCaa > 1:396440/1‑148 (MQ=255) ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCg < 2:461214/151‑1 (MQ=255) ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCg < 2:93683/151‑1 (MQ=255) cTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGGGGGTTTTTTTTTCACCAGTGAGACGGGGAACAGCTGATTGCCCCTCACCGCCTGGCCCTGAGAGAGTTGCAGCaa > 2:980109/1‑147 (MQ=255) tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGAGTGCGTA‑ATTGGGGCCGCGGGGGGTTTTTTTTTCTCCAGCGAGAAGGGCAACAGCTTATTGCCCTTTTTCCCCTGGCCCTGAGCGAGTTGCCGGAAgggg > 1:1016564/1‑150 (MQ=255) gTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGGGTA‑TTGGGGGCCCGGGGGGGTTTTCTTTTTACCAGAGAGACGGGCAACAGCTGATTGCCCTTTACCGCCTGGCCCTGGGAGAGTTGCAGCAAGCGGTcccc > 1:115527/1‑149 (MQ=255) gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACg < 1:158094/150‑1 (MQ=255) gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACg < 1:282159/150‑1 (MQ=255) gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGc < 2:939021/151‑1 (MQ=255) ccAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt < 1:615143/151‑1 (MQ=255) cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTTGGGGCCCGGGGGGTTTTTTTTTTTTCCCGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCCGGCCCTGAGAGAGGTTCCGCAAGCGGGCCAc > 1:925388/1‑147 (MQ=255) tGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTTGGCGCCCGGGGGGGTTTTCTTTTTACCAGGGAGACGGGCAACAGCTGATTGGCCCTCACCCCCCGGGCCCGAGAGAGGTGCAGCAAACGGGCCACGCT‑gg > 2:1284469/1‑148 (MQ=255) gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGtt < 1:744949/149‑1 (MQ=255) gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTg < 1:1180344/151‑1 (MQ=255) ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGGGCCAGGGGGGGTTTTTTTTTTACCAGTGGGACGGGGAAAAACTGATTGCCCTTCACCCCCCGGCCCTGGGAGAGGTGCAGCAAGCGGGCCCCGCT‑GGTTTGccc > 1:929006/1‑151 (MQ=255) aaTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGA‑TTTGGGGCCAGGGGGGGTTTTCTTTTCACCCGTGAGACGGGCAACAGCTGATTGCCCCTCACCCCCCGGCCCCGAGAGAGGTGCAGCAAGCGGGCCACGCC‑GGGTTGgccc > 2:311511/1‑150 (MQ=255) aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAgg < 1:3718/151‑1 (MQ=255) ggCCAACGCGCGGGGAGAGGCGGTTTTCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCTGGCCCCGAGAGAGTTGCAGCAAGCGGGCCACGCTGGGTTTTccccgccgg > 1:1104995/1‑143 (MQ=255) ggCCAACGCGCGGGGAGAGGCGGTTTGCGGA‑TTTGGGGCCAGGGGGGGTTTTCTTTTTACCCGTGAGACGGGCAACAGCTGATTGCCCTTCCCCCCCCGGCCCTGAGAGAGGTGCAGCAAACGGGCCCCCCC‑GGGTTTCCCCACCAGGCg > 1:848209/1‑150 (MQ=255) accgcgcgGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAAt < 2:545776/149‑1 (MQ=255) gagaGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAt < 1:916881/151‑1 (MQ=255) agagGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtg < 2:645026/151‑1 (MQ=255) agGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggt < 1:576107/151‑1 (MQ=255) ggCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggt < 2:57750/150‑1 (MQ=255) gCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg < 1:238913/151‑1 (MQ=255) cGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg > 2:1305267/1‑150 (MQ=255) ggTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt < 1:665448/151‑1 (MQ=255) gCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcg < 1:1024463/150‑1 (MQ=255) gCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcg < 1:687788/150‑1 (MQ=255) cGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggc < 2:181274/151‑1 (MQ=255) tA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggcgg > 2:555973/1‑151 (MQ=255) tGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGa < 1:321778/150‑1 (MQ=255) ggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGata > 2:1330151/1‑150 (MQ=255) gcgcCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGCCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGAtata < 2:402377/151‑1 (MQ=255) ccAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACa < 2:928985/151‑1 (MQ=255) ccAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACa < 2:205619/151‑1 (MQ=255) cAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACa > 2:563367/1‑150 (MQ=255) cAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGACATAACa > 2:829619/1‑150 (MQ=255) aGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACa > 2:593143/1‑149 (MQ=255) | AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT‑GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACA > NC_000913/366377‑366670 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |