Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,311,924	T→C	Y108C (TAT→TGT)	yciA ←	acyl‑CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,311,924	0	T	C	100.0%	361.8 / NA	89	Y108C (TAT→TGT)	yciA	acyl‑CoA esterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/37);  total (52/37)

TAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCA  >  NC_000913/1311778‑1312070                                                                                                                                                   |                                                                                                                                                   tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGc                                                                                                                                                 <  2:240528/150‑1 (MQ=255)    aaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTg                                                                                                                                             <  1:923006/151‑1 (MQ=255)        aaCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGccc                                                                                                                                          >  2:981354/1‑150 (MQ=255)        aaCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGccc                                                                                                                                          >  1:953264/1‑150 (MQ=255)         aCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCa                                                                                                                                         >  2:1105366/1‑150 (MQ=255)           ggCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAAtt                                                                                                                                      <  2:1382377/151‑1 (MQ=255)           ggCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAAtt                                                                                                                                      <  1:736976/151‑1 (MQ=255)            gCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTg                                                                                                                                     <  2:1052681/151‑1 (MQ=255)               ccaccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGtt                                                                                                                                  >  1:635328/1‑151 (MQ=255)                   cGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGa                                                                                                                              <  1:1018206/151‑1 (MQ=255)                    gAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGAc                                                                                                                             >  2:682627/1‑151 (MQ=255)                      aaTTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGc                                                                                                                           >  1:1210181/1‑151 (MQ=255)                           aGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACtt                                                                                                                      <  2:1062012/151‑1 (MQ=255)                           aGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACtt                                                                                                                      >  1:1361287/1‑151 (MQ=255)                                   gAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCAcc                                                                                                              >  2:969168/1‑151 (MQ=255)                                    aaaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCAccc                                                                                                             >  2:173411/1‑151 (MQ=255)                                      aGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCcac                                                                                                           >  1:732613/1‑151 (MQ=255)                                         cAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACt                                                                                                        >  2:1341308/1‑151 (MQ=255)                                          aaaaGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACtt                                                                                                       <  2:124712/151‑1 (MQ=255)                                          aaaaGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACtt                                                                                                       <  1:738775/151‑1 (MQ=255)                                           aaaGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTc                                                                                                      >  2:924198/1‑151 (MQ=255)                                               ccTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAat                                                                                                   >  1:1370851/1‑150 (MQ=255)                                                cTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAatat                                                                                                 >  1:1205826/1‑151 (MQ=255)                                                cTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAatat                                                                                                 >  1:632304/1‑151 (MQ=255)                                                 tCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATAtt                                                                                                >  1:55139/1‑151 (MQ=255)                                                  ccGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAatat                                                                                                 <  2:1210406/149‑1 (MQ=255)                                                  ccGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAatat                                                                                                 <  2:9767/149‑1 (MQ=255)                                                  ccGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATAtt                                                                                                >  2:687258/1‑150 (MQ=255)                                                  ccGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTa                                                                                               <  2:233714/151‑1 (MQ=255)                                                    gACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAAt                                                                                             >  1:417866/1‑151 (MQ=255)                                                      ccGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGc                                                                                           >  1:194583/1‑151 (MQ=255)                                                      ccGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGc                                                                                           >  2:329603/1‑151 (MQ=255)                                                       cGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCt                                                                                          >  1:119160/1‑151 (MQ=255)                                                        ggAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTg                                                                                         >  1:1358887/1‑151 (MQ=255)                                                           ggCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGAcc                                                                                      >  2:408833/1‑151 (MQ=255)                                                             cTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCg                                                                                     >  2:601558/1‑150 (MQ=255)                                                               tttGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGAt                                                                                   <  1:903888/150‑1 (MQ=255)                                                               tttGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGAt                                                                                   <  1:884148/150‑1 (MQ=255)                                                                ttGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATgt                                                                                 >  1:484862/1‑151 (MQ=255)                                                                      aTTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATgtcgtc                                                                             <  1:1400061/149‑1 (MQ=255)                                                                         aCTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCttt                                                                        <  1:453912/151‑1 (MQ=255)                                                                          cTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTc                                                                       >  2:1294003/1‑151 (MQ=255)                                                                            cAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTg                                                                     <  2:513123/151‑1 (MQ=255)                                                                             aaCAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTgg                                                                    >  1:410440/1‑151 (MQ=255)                                                                                aGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGaca                                                                 <  2:118725/151‑1 (MQ=255)                                                                                     aGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCg                                                            >  2:774755/1‑151 (MQ=255)                                                                                     aGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCg                                                            <  1:334490/151‑1 (MQ=255)                                                                                      ggCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGt                                                           <  1:1409839/151‑1 (MQ=255)                                                                                            aGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCAt                                                       >  2:1212211/1‑149 (MQ=255)                                                                                               ttttCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATcgc                                                    >  2:227732/1‑149 (MQ=255)                                                                                                  tCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGcac                                               >  1:1083416/1‑151 (MQ=255)                                                                                                   ccTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGcac                                               >  1:542904/1‑150 (MQ=255)                                                                                                       cAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCAcacca                                           <  2:312815/150‑1 (MQ=255)                                                                                                        aGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACcaca                                         <  1:764428/151‑1 (MQ=255)                                                                                                         ggATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACAt                                        <  2:1361260/151‑1 (MQ=255)                                                                                                          gATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATc                                       <  1:142419/151‑1 (MQ=255)                                                                                                             cAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGcc                                    >  1:1283144/1‑151 (MQ=255)                                                                                                              aaCCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCg                                   <  1:1249334/151‑1 (MQ=255)                                                                                                               aCCGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCg                                   >  1:1173574/1‑150 (MQ=255)                                                                                                                ccGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGAc                                 <  1:1050468/151‑1 (MQ=255)                                                                                                                ccGCGACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGAc                                 >  1:1045137/1‑151 (MQ=255)                                                                                                                  gcgACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCg                               <  1:1105389/151‑1 (MQ=255)                                                                                                                   cgACATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGc                              >  2:1341372/1‑151 (MQ=255)                                                                                                                     aCATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCaa                            <  1:198659/151‑1 (MQ=255)                                                                                                                      cATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAAc                           <  1:718454/151‑1 (MQ=255)                                                                                                                      cATACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAAc                           >  2:1365993/1‑151 (MQ=255)                                                                                                                       aTACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAAcc                          <  1:1199678/151‑1 (MQ=255)                                                                                                                       aTACTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAAcc                          >  1:1008229/1‑151 (MQ=255)                                                                                                                         aCTTAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCg                         <  2:1045116/150‑1 (MQ=255)                                                                                                                           ttAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGcc                      >  2:132522/1‑151 (MQ=255)                                                                                                                           ttAAATAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGcc                      >  1:457949/1‑151 (MQ=255)                                                                                                                             aaaTAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCg                     <  1:981375/150‑1 (MQ=255)                                                                                                                             aaaTAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCg                     <  1:927025/150‑1 (MQ=255)                                                                                                                             aaaTAATGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGAGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGt                    >  2:156177/1‑151 (MQ=255)                                                                                                                              aataatGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGt                    >  2:556230/1‑150 (MQ=255)                                                                                                                                taatGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTa                   >  2:1024125/1‑149 (MQ=255)                                                                                                                                taatGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTa                   >  1:890020/1‑149 (MQ=255)                                                                                                                                  atGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGa                >  1:1374416/1‑150 (MQ=255)                                                                                                                                  atGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGa                >  1:1061235/1‑150 (MQ=255)                                                                                                                                  atGCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGa                >  2:823194/1‑150 (MQ=255)                                                                                                                                    gCTTCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGaaa              <  1:1093055/150‑1 (MQ=255)                                                                                                                                       tCTGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTc           >  1:45837/1‑150 (MQ=255)                                                                                                                                         tGTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCAtt        >  1:1356365/1‑151 (MQ=255)                                                                                                                                          gTCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCAt         <  2:484850/149‑1 (MQ=255)                                                                                                                                           tCGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTcc      >  1:252161/1‑151 (MQ=255)                                                                                                                                            cGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCt     <  1:589231/151‑1 (MQ=255)                                                                                                                                            cGCTTTACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCt     >  2:1134059/1‑151 (MQ=255)                                                                                                                                               tttACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTc   >  2:704294/1‑150 (MQ=255)                                                                                                                                                ttACAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCa  <  2:1370824/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAATGCTGACCGATGTCGTCCCTTTCTGGACACAGCGTGCATAGCAGCACACCACATCGCCGACCGCAACCGGCCGTAAGAAAGTCATTCCTTCA  >  NC_000913/1311778‑1312070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑