Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,400,417	A→T	I44F (ATC→TTC)	hfq →	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,417	0	A	T	100.0%	326.1 / NA	80	I44F (ATC→TTC)	hfq	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (40/40);  total (40/40)

TATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAAT  >  NC_000913/4400271‑4400560                                                                                                                                                   |                                                                                                                                                tataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCt                                                                                                                                             >  1:283640/1‑151 (MQ=255)    aaGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGtt                                                                                                                                          <  2:818259/151‑1 (MQ=255)    aaGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGtt                                                                                                                                          >  2:122278/1‑151 (MQ=255)     aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTg                                                                                                                                         <  2:583722/151‑1 (MQ=255)     aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTg                                                                                                                                         >  2:482292/1‑151 (MQ=255)     aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTg                                                                                                                                         <  2:1096758/151‑1 (MQ=255)      ggAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGa                                                                                                                                        <  1:659528/151‑1 (MQ=255)       gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGa                                                                                                                                        >  2:378505/1‑150 (MQ=255)         aaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATTGAGTCTTTTGATCAGTTCGTGTTCCTGTTGaaaa                                                                                                                                     <  1:98704/151‑1 (MQ=255)         aaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGaaa                                                                                                                                      >  2:1101109/1‑150 (MQ=255)          aaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGaaaaa                                                                                                                                    <  1:517158/151‑1 (MQ=255)          aaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGaaaaa                                                                                                                                    <  2:945188/151‑1 (MQ=255)           agagagAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGCATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAac                                                                                                                                   >  1:716979/1‑151 (MQ=255)            gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAaca                                                                                                                                  <  2:1074269/151‑1 (MQ=255)            gagagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAaca                                                                                                                                  <  2:428917/151‑1 (MQ=255)             agagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAacac                                                                                                                                 >  2:812113/1‑151 (MQ=255)                       tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGcc                                                                                                                         >  2:114731/1‑149 (MQ=255)                         aGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGAt                                                                                                                     <  2:159195/151‑1 (MQ=255)                          ggggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATg                                                                                                                    <  2:762235/151‑1 (MQ=255)                           gggCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATgg                                                                                                                   >  1:315121/1‑151 (MQ=255)                             gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                                                                                                 >  2:1330392/1‑151 (MQ=255)                              cAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGttt                                                                                                                <  1:67523/151‑1 (MQ=255)                                 tCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                                                                                                              >  1:46649/1‑150 (MQ=255)                                    ttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACaa                                                                                                            <  1:1029622/149‑1 (MQ=255)                                      aCAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                                                                                                        >  1:474974/1‑151 (MQ=255)                                      aCAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                                                                                                        <  2:334612/151‑1 (MQ=255)                                      aCAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                                                                                                        <  2:858802/151‑1 (MQ=255)                                         aGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgcg                                                                                                     <  1:551054/151‑1 (MQ=255)                                            tCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAtt                                                                                                  >  2:1016251/1‑151 (MQ=255)                                             ccGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAtt                                                                                                  <  1:363839/150‑1 (MQ=255)                                             ccGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAtt                                                                                                  <  2:223221/150‑1 (MQ=255)                                             ccGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAtt                                                                                                  <  1:319809/150‑1 (MQ=255)                                              cGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc                                                                                                >  2:778487/1‑151 (MQ=255)                                                  ccTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt                                                                                            <  2:228671/151‑1 (MQ=255)                                                     gAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgtt                                                                                         <  2:539700/151‑1 (MQ=255)                                                       aCGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtgttgt                                                                                       >  1:163232/1‑151 (MQ=255)                                                             tGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc                                                                                 >  2:1006849/1‑151 (MQ=255)                                                                 tCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCg                                                                              <  1:715651/150‑1 (MQ=255)                                                                   gggAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGccc                                                                           <  1:614265/151‑1 (MQ=255)                                                                       aCGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGtt                                                                       >  1:415810/1‑151 (MQ=255)                                                                         gtgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtc                                                                     <  1:818423/151‑1 (MQ=255)                                                                          tgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtct                                                                    >  1:954796/1‑151 (MQ=255)                                                                           gtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtctc                                                                   >  2:393607/1‑151 (MQ=255)                                                                              ccAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatc                                                                >  2:351182/1‑151 (MQ=255)                                                                                 gTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcac                                                              >  1:641120/1‑150 (MQ=255)                                                                                 gTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcac                                                              >  1:1154408/1‑150 (MQ=255)                                                                                    tCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGt                                                           <  2:1297641/150‑1 (MQ=255)                                                                                       atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaac                                                        >  1:535287/1‑150 (MQ=255)                                                                                            tttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGcc                                                  >  2:461270/1‑151 (MQ=255)                                                                                               ggTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCgg                                                <  2:88564/150‑1 (MQ=255)                                                                                                 tGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTgg                                             >  2:920141/1‑151 (MQ=255)                                                                                                  gAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGc                                            <  1:629628/151‑1 (MQ=255)                                                                                                   aaTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                                           <  2:810295/151‑1 (MQ=255)                                                                                                      ggTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGt                                         >  2:572875/1‑150 (MQ=255)                                                                                                      ggTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGt                                         >  1:986083/1‑150 (MQ=255)                                                                                                       gTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTAc                                       >  1:720659/1‑151 (MQ=255)                                                                                                         aTTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACca                                     <  1:889866/151‑1 (MQ=255)                                                                                                            aaGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagc                                   <  1:1026374/150‑1 (MQ=255)                                                                                                              gCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGt                                >  1:222500/1‑151 (MQ=255)                                                                                                                 gCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTa                               <  1:36811/149‑1 (MQ=255)                                                                                                                       gCAAATCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcat                       >  2:329866/1‑151 (MQ=255)                                                                                                                         aaaTCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatca                     <  1:1291325/151‑1 (MQ=255)                                                                                                                          aaTCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatcat                    >  2:807453/1‑151 (MQ=255)                                                                                                                          aaTCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatcat                    <  2:954775/151‑1 (MQ=255)                                                                                                                          aaTCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatcat                    >  2:1242872/1‑151 (MQ=255)                                                                                                                          aaTCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatcat                    <  1:758379/151‑1 (MQ=255)                                                                                                                           aTCGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATg                   <  1:122280/151‑1 (MQ=255)                                                                                                                             cGAGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGt                 >  2:624191/1‑151 (MQ=255)                                                                                                                               aGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTag               <  2:645914/151‑1 (MQ=255)                                                                                                                               aGTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTag               <  1:793660/151‑1 (MQ=255)                                                                                                                                gTCTTTTGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagc              >  1:1068198/1‑151 (MQ=255)                                                                                                                                    tttGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagcagc           <  1:438893/150‑1 (MQ=255)                                                                                                                                    tttGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTagcag            <  1:930422/149‑1 (MQ=255)                                                                                                                                     ttGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgc         >  2:355512/1‑151 (MQ=255)                                                                                                                                      tGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcg        >  2:1371129/1‑151 (MQ=255)                                                                                                                                      tGATCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcg        >  2:1190549/1‑151 (MQ=255)                                                                                                                                         tCAGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAgcgcgc       >  1:811884/1‑149 (MQ=255)                                                                                                                                           aGTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGa    >  1:1320281/1‑150 (MQ=255)                                                                                                                                            gTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAAt  <  2:789265/151‑1 (MQ=255)                                                                                                                                            gTTCGTGTTCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAAt  >  1:121435/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                TATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAGCAGCGCGCAGAAT  >  NC_000913/4400271‑4400560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑