Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 380,022 (G)10→11 intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0121.G82.3% 188.7 / 39.2 62intergenic (‑131/+57)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (8/3);  new base G (31/20);  total (39/23)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

TCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCC  >  NC_000913/379865‑380157
                                                                                                                                                     |                                                                                                                                                     
tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑ggg                                                                                                                                                  >  1:1323534/1‑151 (MQ=255)
tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑gg                                                                                                                                                   <  2:207615/150‑1 (MQ=255)
  cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑ggggg                                                                                                                                                <  1:237213/151‑1 (MQ=255)
     aGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑gggggggg                                                                                                                                             <  2:968650/151‑1 (MQ=255)
      gTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑ggggggggg                                                                                                                                            <  1:352699/151‑1 (MQ=255)
        aCTGGGCATTTCCCACCTCATCATCTGCATGCAATATAATAAAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑ggggggggg                                                                                                                                            <  2:469477/149‑1 (MQ=255)
          tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑gggggggggggct                                                                                                                                        >  1:46980/1‑148 (MQ=255)
           gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑gggggggggggcta                                                                                                                                       <  2:689138/151‑5 (MQ=255)
                      aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCATTGCATACCCCCCTCTAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGCGGAAA‑‑‑ggggggggggagct                                                                                                                                       >  2:1470203/1‑136 (MQ=255)
                        cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                          >  1:1014535/1‑151 (MQ=255)
                         tcatcaTCCGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                          >  1:646929/1‑151 (MQ=255)
                           atcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                                                                       >  1:426080/1‑151 (MQ=255)
                              atcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGgcccc                                                                                                                     >  1:279145/1‑149 (MQ=255)
                                cttcatgcaATATACTAAAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGGGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGgccgc                                                                                                                     <  1:986943/145‑1 (MQ=255)
                                 tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGGCGCCCCg                                                                                                                 >  2:102801/1‑151 (MQ=255)
                                   catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                                >  2:397044/1‑150 (MQ=255)
                                     tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                               >  2:526402/1‑148 (MQ=255)
                                       caATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATTGGCAGGGAAGGCCGCCCAGGaaa                                                                                                             >  2:336781/1‑147 (MQ=255)
                                        aaTATATTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                                                        <  1:793478/151‑1 (MQ=255)
                                        aaTATACTATAGGGGGGTATTTTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                                                                           <  2:1325726/149‑1 (MQ=255)
                                        aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                         >  2:277057/1‑151 (MQ=255)
                                             aCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                                                                     >  2:1125217/1‑151 (MQ=255)
                                               tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgagggcgg                                                                                                    >  2:30543/1‑149 (MQ=255)
                                                ataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                                                                                 <  2:1110688/151‑1 (MQ=255)
                                                ataGGGGGGTATTCTATATATAAATGCATACCCCCCTATAGTATATAACACTGGGGAATAAAATATATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGATAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                                  <  2:322450/150‑1 (MQ=255)
                                                aaaGGGGGGTATTTTATTTGTCAATGCATACCCCCCAATATTAAATACCCCTGGAAAATAAAATTTATCCGGTAAATGTGGTCGAAAAACAAAGAGGAAGG‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                                                                                 <  1:102816/149‑1 (MQ=255)
                                                   ggggggTATTCTATATGTCAATCAATACCCCCCTAAAGTATATAACCCTGGAAAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGGGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                                                               <  1:247963/150‑1 (MQ=255)
                                                      gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                                                                           <  2:1165836/151‑1 (MQ=255)
                                                          aTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCCCCCCGGATACAGGGCGGCAAAAGGAAt                                                                                          >  2:68345/1‑148 (MQ=255)
                                                           ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAAAGCGGGCGGCAGAAGGAAt                                                                                          >  2:1228680/1‑149 (MQ=255)
                                                           ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAAACGGCAGGGGAAGCCGCCCCCGGTAGGGGGGGGGCGGAAGGa                                                                                            >  2:409748/1‑145 (MQ=255)
                                                           ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACGGGAGAATAAAATTTATCCGGTGAATGGGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                                                        <  2:1159876/149‑1 (MQ=255)
                                                                tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                                 >  2:920861/1‑151 (MQ=255)
                                                                tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                                 >  1:719341/1‑151 (MQ=255)
                                                                 atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                                                                >  1:826015/1‑151 (MQ=255)
                                                                 atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                                 <  2:891877/150‑1 (MQ=255)
                                                                    tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                             >  1:459713/1‑151 (MQ=255)
                                                                     cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGTTAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                             >  1:414/1‑151 (MQ=255)
                                                                             aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                                                                     >  1:868685/1‑151 (MQ=255)
                                                                              ccccccTATAGTATATAACACTGGAGAATAAAATTTTTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg                                                                    <  2:1137098/149‑1 (MQ=255)
                                                                              ccccccTATAGTATATAACACGGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                                                                   <  1:1007171/151‑1 (MQ=255)
                                                                                 cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGTAGGCCCCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGCCAGCCGGGCTGc                                                                >  2:1190396/1‑151 (MQ=255)
                                                                                  ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGTTAATCGCCGGGGAGGGCCCCCCCGGATAGGGGGCGGCAGAAGGATTCAGATTTTCCGGGCCAGACgg                                                                     >  1:369008/1‑145 (MQ=255)
                                                                                        gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                                                         <  2:304603/151‑1 (MQ=255)
                                                                                         tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                                                         >  2:196270/1‑150 (MQ=255)
                                                                                            ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                     >  1:1045310/1‑151 (MQ=255)
                                                                                                cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCGGGGAAGGCCGCCCCGGATAGCGGGGGGCAGAAGGAATCAGATTTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGtt                                                 >  2:338530/1‑151 (MQ=255)
                                                                                                 acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGtt                                                 >  1:664283/1‑150 (MQ=255)
                                                                                                 acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGACAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGtt                                                 >  1:750918/1‑150 (MQ=255)
                                                                                                   tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                              >  2:241950/1‑150 (MQ=255)
                                                                                                    ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                              <  2:833026/150‑1 (MQ=255)
                                                                                                    ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaa                                               <  1:1008566/149‑1 (MQ=255)
                                                                                                     gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                            >  1:1378934/1‑151 (MQ=255)
                                                                                                     gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc                                           >  2:1380966/1‑151 (MQ=255)
                                                                                                        aaaaaaaTTTATCCGGTGAATGTGGGCGAAAAACAAAGAGGAAAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatca                                          <  1:1365293/148‑1 (MQ=255)
                                                                                                        aaTAAAATTTATCCGGTGAATGGGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatca                                          <  2:474938/150‑1 (MQ=255)
                                                                                                         aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCCAGTTGCAGACCGTTAAAatcat                                         >  2:1335079/1‑150 (MQ=255)
                                                                                                          tAAAATTTATCCGGTGAATGGGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc                                        <  1:55974/150‑1 (MQ=255)
                                                                                                            aaaTTTATGCGGTGAATGTGGTGGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGACGTCCACCAAGGAAAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCAGCAAGTGGCGGACCGTTAAAATCATCgg                                      >  2:1404919/1‑150 (MQ=255)
                                                                                                                ttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg                                 >  1:1372328/1‑151 (MQ=255)
                                                                                                                   tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt                               <  1:700449/150‑1 (MQ=255)
                                                                                                                   tCCGGTGAAGGTGGGCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg                                <  2:972295/149‑1 (MQ=255)
                                                                                                                    ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg                                >  1:151848/1‑148 (MQ=255)
                                                                                                                    ccGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt                             >  1:1139030/1‑150 (MQ=255)
                                                                                                                      ggTGAATGTGGTCGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                           <  2:883920/151‑1 (MQ=255)
                                                                                                                       gTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGTCAAAGGGGGGGGAAGGCCCCCCCCGGATAGGGGGGGGGAAAGGGAATAAAAATTTCCGGGCCAGAGGGGCTGCAGTTTGCAGCCCGTTAAACTCCTGGTTTGGGgt                               >  2:1051020/1‑146 (MQ=255)
                                                                                                                          aaTGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAcc                       >  2:600118/1‑150 (MQ=255)
                                                                                                                                  cGGAAAACAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt                >  1:42612/1‑150 (MQ=255)
                                                                                                                                     aaaaCAAAGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCt            <  2:77151/151‑1 (MQ=255)
                                                                                                                                       aaaaaaGAGGAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGc          <  1:888773/148‑1 (MQ=255)
                                                                                                                                               ggAAAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGcc  >  1:904169/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCC  >  NC_000913/379865‑380157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: