Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	52.8%	113.0 / 156.7	89	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/40);  new base G (47/0);  total (49/40)
Fisher's exact test for biased strand distribution p-value = 2.59e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-16

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354138‑354435                                                                                                                                                       |                                                                                                                                                      aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGt                                                                                                                                                       <  2:402970/151‑1 (MQ=255)    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGa                                                                                                                                                     <  1:791810/150‑1 (MQ=255)    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGa                                                                                                                                                     <  1:242946/150‑1 (MQ=255)      ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                                  <  1:76605/151‑1 (MQ=255)         aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATg                                                                                                                                               <  2:1298308/151‑1 (MQ=255)                aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATgcg                                                                                                                                             >  2:1232025/1‑146 (MQ=255)                  gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAGATGCGC‑TG‑aaa                                                                                                                                     >  1:257849/1‑150 (MQ=255)                   cTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGGGC‑TG‑AAAgg                                                                                                                                   >  1:408752/1‑151 (MQ=255)                    tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGC‑TG‑AAAggg                                                                                                                                  >  2:218886/1‑151 (MQ=255)                     gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGAGC‑CG‑GAAgggg                                                                                                                                 >  2:611418/1‑150 (MQ=255)                     gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGC‑TG‑GAAggg                                                                                                                                  >  2:1348681/1‑150 (MQ=255)                      aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGC‑TG‑GAAggg                                                                                                                                  >  2:1305804/1‑149 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGC‑TG‑GGAGGGCgggg                                                                                                                             >  2:1213401/1‑150 (MQ=255)                          ttATCACTTTATTGTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGAGAGGGGACC‑TG‑AAAGTGCGggt                                                                                                                             >  1:921142/1‑150 (MQ=255)                          ttATCACTTTATTCTCGGGCGCACTGACGGTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGGGATTGAAGCGAGTATCCCAAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAGGCTA‑CG‑TAAGGGCAggcg                                                                                                                            >  1:634691/1‑149 (MQ=255)                           tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGGAGAGGCGCGGG‑AAAGGGCggggg                                                                                                                            >  2:640527/1‑149 (MQ=255)                            aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAggtggc                                                                                                                          <  1:611491/151‑1 (MQ=255)                            aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAggtgg                                                                                                                           <  2:667567/150‑1 (MQ=255)                              cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAggtggcg                                                                                                                         <  2:736384/150‑1 (MQ=255)                               aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGGGC‑TG‑AAAAGGCAgggggcgg                                                                                                                        >  1:545508/1‑150 (MQ=255)                                cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAggtggcggt                                                                                                                       <  2:1175375/150‑1 (MQ=255)                                 tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAGGGGC‑TG‑AAAGGGGAggtgggggggg                                                                                                                     >  1:1237149/1‑151 (MQ=255)                                  ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAggtggcggtg                                                                                                                      <  1:1029364/149‑1 (MQ=255)                                      tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGtt                                                                                                                 <  1:1088124/150‑1 (MQ=255)                                      tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGtt                                                                                                                 <  2:1414684/150‑1 (MQ=255)                                      tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGC‑TG‑GAAGGGGAgggggcggggggggtt                                                                                                                >  2:130645/1‑146 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGttt                                                                                                                <  1:166980/148‑1 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                              <  2:1317741/150‑1 (MQ=255)                                            gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                          <  2:636006/151‑1 (MQ=255)                                                   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGC‑TG‑AAAGGGGAGGGGGGGGGGGCGTTTGTCATTCCGaa                                                                                                     >  1:1009509/1‑149 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                              <  2:1131761/150‑1 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGG‑TG‑GAAAGGCAGGGGGCGGGGGGGGTTGTCATTCCCGAagaga                                                                                                >  2:1207212/1‑148 (MQ=255)                                                          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGC‑TG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCg                                                                                             >  1:36655/1‑150 (MQ=255)                                                          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGC‑TG‑GAAGGGGAGGGGGGGGGGGGGGTTGGTATTCCCGAagaggggg                                                                                             >  2:157428/1‑146 (MQ=255)                                                             ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGAAGCGC‑TGTAAAGGGC‑GGGGGGGGGGGGGGTTGGCATTCCCAAAGAGAGCGAc                                                                                           >  1:1007335/1‑149 (MQ=255)                                                              tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                          <  2:1007241/149‑1 (MQ=255)                                                                aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                      <  1:493980/151‑1 (MQ=255)                                                                  gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                    <  2:24089/151‑1 (MQ=255)                                                                  gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGC‑TG‑GAAGGGGAGGGGGGGGGGGGGGTTTTCATTTCGGAAAAGAGGGACAGGCTgg                                                                                    >  2:1181867/1‑151 (MQ=255)                                                                   ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGGAAGACGCCC‑GG‑GAAGGGGAGGGGGGGGGGGCGCTTGTGGTTCCGGAaaagggagagacgcagg                                                                                    >  2:173810/1‑137 (MQ=255)                                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                   <  1:163219/150‑1 (MQ=255)                                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                 <  1:1386648/151‑1 (MQ=255)                                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGC‑TG‑AAAGGGCAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTCTgg                                                                                    >  1:812461/1‑148 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGAAAGATGCGC‑TT‑GAAGGGCCGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTgg                                                                                    >  1:477003/1‑146 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                               <  2:828331/151‑1 (MQ=255)                                                                         ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                             <  2:118833/151‑1 (MQ=255)                                                                            aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGC‑TG‑AAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCGAAAGAGGGCGGTAGGCTGGGAGATCggg                                                                           >  1:1239056/1‑148 (MQ=255)                                                                              cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                                         <  1:1038696/150‑1 (MQ=255)                                                                              cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGGGG‑TG‑AAAGGGGAGGGGGGGGCGGCGGTTGTAATTCCGAAAGAGGGCGACAGTCTGGAAGATCGTg                                                                           >  2:582543/1‑148 (MQ=255)                                                                                  tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGGAAGATGCGC‑TG‑AAAGGGCAGGTGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                      >  1:1225327/1‑149 (MQ=255)                                                                                    gggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc                                                                  <  2:1379397/151‑1 (MQ=255)                                                                                     tgACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAGATGCGC‑TG‑AAAAGGCAGGGGGCGGTGGCGGTTTTTCTTCCGAAAGAGAGCGGAAGTCTGGGAGAACGGGATGTGgcg                                                                   >  2:655987/2‑149 (MQ=255)                                                                                         gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGC‑TG‑AAAGGGGAGGTGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGCGCACTc                                                              >  1:964233/1‑150 (MQ=255)                                                                                         gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGC‑TG‑AAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                              >  1:1459674/1‑150 (MQ=255)                                                                                          cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGC‑TG‑GAAAGGGAGGTGGCGGTGGCGCTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGAGCGGGATGTAGGGCAccc                                                              >  2:952747/1‑147 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGC‑TG‑GAAGGGGAGGGGGGGGGGGGGGGTGTCCTTTCGAAAAAGAGGGAGAGGCTGGAAGATCGTGATGTGGCGCCCCCGc                                                            >  2:959993/1‑150 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGC‑TG‑AAAGGGCAGGGGGGGGGGGCGGTTTTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGGGATGGGGGGCCCCCGc                                                            >  2:1105238/1‑150 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGC‑TG‑AAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGGGCACTc                                                              >  1:4430/1‑148 (MQ=255)                                                                                             gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGAAAAATGCGC‑TG‑GAAGGGGAGGGGGGGGGGGGGTTTGTCATTCCGGAAGAGAGCGACAGACTGGAAGATCGTGGTGGGGCGCaaacg                                                             >  1:923888/1‑143 (MQ=255)                                                                                                 ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGC‑TG‑AAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCCCTCGCAaga                                                        >  2:232093/1‑148 (MQ=255)                                                                                                    aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGC‑TG‑AAAGGGGAGGTGGGGGGGGGGGTTGTCATTTCGCAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCGCTCGCAaga                                                        >  2:36163/1‑145 (MQ=255)                                                                                                    aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTCGGGGGAAAAAAAAGC‑CG‑TACTGGCCGGTGGAGGTGGCGTGTGTTATTACGAAAAAAGGCGTCATTTTGGAAAAATCAGCAGTGGCGCAAACGCAACAGAAg                                                    >  1:691964/1‑149 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                                <  2:351599/149‑1 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                            <  1:640601/151‑1 (MQ=255)                                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAAAGCGC‑TG‑AAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGGAAGAGAGCGACAGTCTGGGAGAACGTGATGTGGGGCACTCGCAAGAGAAGGCGATTAt                                            >  2:1356279/1‑150 (MQ=255)                                                                                                               tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGC‑TG‑GAAGGGCAGGTGGGGGGGGCGTTTGTCATTCCGAAAGAGAGCGAAAGTCTGGAAGAACGTGATGGGGGGCACTCGCAAGAGAAAGGGGTTATGgcg                                        >  2:118304/1‑150 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                          <  1:547805/147‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                        <  2:1466506/149‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcg                                        <  1:50219/149‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc                                         <  1:952838/148‑1 (MQ=255)                                                                                                                 cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                                     <  2:531320/151‑1 (MQ=255)                                                                                                                   gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                   <  1:822803/151‑1 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGa                                <  1:285262/151‑1 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGAAAGATGGGC‑TG‑GAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGGCCGGAAGGTCGGGGTGTGGCGCACTCGCAAGAGAAGGGGGTTATGGCGCtggggg                                 >  1:1283157/1‑150 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGC‑TG‑GAAGGGGAGGGGGGGGGGGCGTTTGTCATTTCGAAAAAGAGCGACAGTATGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                                 >  1:1006577/1‑150 (MQ=255)                                                                                                                        ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACa                              <  2:444540/151‑1 (MQ=255)                                                                                                                          gggCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGC‑TG‑GCAAGGCCGGTGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGCAGATCGTGGTGTGGCGCCCTCCCCAAAGGAGGCGATTATGGCGCTGGGGGGCAg                             >  1:706127/1‑150 (MQ=255)                                                                                                                           ggCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGC‑TG‑AAAAGGCAGGTGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGGGGACAGcc                           >  1:485669/1‑151 (MQ=255)                                                                                                                             cGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGC‑TG‑AAAAGGGAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCCGGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGGCAGcc                           >  1:526629/1‑149 (MQ=255)                                                                                                                              gTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGa                        <  1:437214/151‑1 (MQ=255)                                                                                                                              gTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGC‑TG‑AAAAGGGCGGGGGGGGGGGGGGTTGTTGTTCCCACAGAGAGGGGCGGTTGGGGAGGTGGGGGTGTGGCGGGGCCGCAAGAGAAAGGGATTATGGGGGtggtgg                                 >  2:1292651/1‑142 (MQ=255)                                                                                                                                tGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGC‑TG‑AAAGGGGAGGTGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGGGGACAGCCAGaat                      >  1:1432689/1‑149 (MQ=255)                                                                                                                                tGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGC‑TG‑AAAAGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGGGGACAGcc                           >  1:473111/1‑146 (MQ=255)                                                                                                                                         ggCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt             <  2:797036/151‑1 (MQ=255)                                                                                                                                            ggtggtTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg            <  2:68598/149‑1 (MQ=255)                                                                                                                                                  tGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc     <  1:1067167/150‑1 (MQ=255)                                                                                                                                                  tGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc     <  2:1009415/150‑1 (MQ=255)                                                                                                                                                  tGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg    >  2:540634/1‑151 (MQ=255)                                                                                                                                                   ggggTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg   <  2:1283053/151‑1 (MQ=255)                                                                                                                                                    gggTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc  >  1:62164/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                      ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGC‑TG‑AAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354138‑354435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑