Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	43.8%	132.6 / 26.8	80	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (4/41);  new base G (35/0);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.42e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730                                                                                                                                                   |                                                                                                                                                         cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTc                                                                                                                                                       <  1:637834/149‑1 (MQ=11)   cTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGTA‑GGGGGGGGTTCG‑aa                                                                                                                                                   >  1:1179429/1‑150 (MQ=12)       gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AAccccc                                                                                                                                              <  2:1194410/151‑1 (MQ=35)       gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AAcccc                                                                                                                                               <  2:101842/150‑1 (MQ=35)        tttaCGGTCTGTCCAAGTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑aa                                                                                                                                                   <  2:1249238/142‑1 (MQ=17)          cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGa                                                                                                                                            <  1:819375/150‑1 (MQ=38)          cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAt                                                                                                                                           <  2:201466/151‑1 (MQ=38)             ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACg                                                                                                                                        <  1:1288054/151‑1 (MQ=40)                  gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGtt                                                                                                                                      <  2:384076/148‑1 (MQ=255)                    ccAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTTG‑AAccccccccaaggt                                                                                                                                      >  2:910164/1‑138 (MQ=11)                      aaCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑AACCCCCCCTACGTTGCCGcat                                                                                                                               >  2:1078174/1‑151 (MQ=11)                       aCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCGTACGtttgcggat                                                                                                                               >  1:442051/1‑143 (MQ=11)                         tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTG‑CACCCCCCGCACGTTTCCGgata                                                                                                                              >  1:1162938/1‑149 (MQ=11)                         tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGG‑AACCCCC‑ACACGTTTCCGgat                                                                                                                               >  2:674694/1‑148 (MQ=11)                          ggCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCCTACGTTGCCGtat                                                                                                                               >  2:1191800/1‑147 (MQ=255)                          ggCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATaca                                                                                                                            <  2:785821/150‑1 (MQ=255)                          ggCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATaca                                                                                                                            <  1:1283438/150‑1 (MQ=255)                                 gCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑Cttt                                                                                                                     <  1:1049128/149‑1 (MQ=255)                                 gCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTcc                                                                                                                   <  1:257621/151‑1 (MQ=255)                                  ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCCTACGGTTCCGCATACACA‑Cctt                                                                                                                     >  1:212873/1‑148 (MQ=14)                                  ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAAAATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCa                                                                                                                  <  2:332874/151‑1 (MQ=255)                                       tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTCG‑GAACCCCCCTACGCTGCCGGATACAAA‑Cttt                                                                                                                     >  1:647143/1‑143 (MQ=14)                                          cGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑GACCCCCCAGACGTTGCCGCGTACACA‑CTTTTCCGGCGTg                                                                                                            >  1:29059/1‑149 (MQ=14)                                             aCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCt                                                                                                       <  1:642051/151‑1 (MQ=255)                                              ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCtt                                                                                                      <  2:310193/151‑1 (MQ=255)                                               cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCATACGTTTGCCTATACACA‑ATTTCCAGGCGGGGTCCtt                                                                                                      >  1:1219325/1‑150 (MQ=17)                                                  ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTG‑AACCCCCCCCACATTTCCCTATACACA‑CTTTTCAGGCGGGCTCCTTCCGc                                                                                                  >  2:850774/1‑151 (MQ=14)                                                        ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCg                                                                                            <  1:968213/151‑1 (MQ=255)                                                           ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑AACCCCCCATACGTTTCCGGATACACA‑CTTTTCAGGCGTGGTCCTTCCGCCCCTc                                                                                             >  1:124206/1‑147 (MQ=17)                                                            tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACG‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGac                                                                                         <  1:1021906/150‑1 (MQ=255)                                                               cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑AACCCCCCCTAACTTGCCGTATACACA‑CCTTCCAGGCGGGGTCCTTCTGCCACTCGGGCAcc                                                                                      >  1:624491/1‑150 (MQ=17)                                                                  ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑Ca                                                                                  <  2:1351157/150‑1 (MQ=255)                                                                   cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGTTTG‑AACCCCCCCAACCGGGGCGCTTACACC‑CTTTTCCGGCGTGGTCCTTCTGCCAACCCGAAACCT‑cccc                                                                                >  2:1141359/1‑151 (MQ=2)                                                                        ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTTG‑AAACCCCCCTACGTTGCCGTATAAACA‑CTTTTCCGGCGGGCTCCTTCAGCCACTCgggaaccccccccaa                                                                              >  2:1471800/1‑136 (MQ=14)                                                                           gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑GACCCCCCCTACATTGCCCTATACACA‑CCTTTCCGGCGGGCTCCCTCCGCCACCCCGGCAccccccccaaattt                                                                          >  2:861798/1‑138 (MQ=9)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AAACCCCCATACGTTGCCGGATACACA‑CTTTCCAGGCGGGGTCCTTCCGCCACCCGGGCACCC‑CACCaaaattt                                                                         >  1:988454/1‑144 (MQ=14)                                                                              tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AAACCCCCATACGTTGCCCTATACAAA‑CTTTTCCGGCGTGCTCCTTCCGCCACTCCGACACCC‑CCCCCAAATTTCGtt                                                                     >  1:1052468/1‑151 (MQ=14)                                                                              tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑AAACCCCCCTACATTTTTGTATAAACA‑CCTTCTAGGCGCGGCCCTTTTCACACTCGGGCACCTCCCCCAAAttttgtt                                                                      >  1:1032679/1‑146 (MQ=2)                                                                              tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGG‑GGGGGGGGTTCG‑AACCCCCCATACATTGCCGTATAAACA‑CTTTTCCGGCGGGCTCCTTCAGCCACCCCGGCACCC‑CACCCAATTGTCGtt                                                                     >  2:1250444/1‑151 (MQ=14)                                                                               gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGtt                                                                     <  2:369214/150‑1 (MQ=255)                                                                               gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTc                                                                    <  2:397906/151‑1 (MQ=255)                                                                                cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTcc                                                                   <  1:262631/151‑1 (MQ=255)                                                                                 aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCATAACTTTCCCGATAAACA‑ACTTCCAGGCGTGCCCCCTCAGCCACCCCGGCAACC‑CACCAAAATTTCGtt                                                                     >  1:447291/1‑148 (MQ=9)                                                                                    ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCCCTACGTTGCCGTATACACA‑CTTTTCAGGCGTGCTCCTTCAGCCCCCCGGACACCC‑CACCAAAATTTCGTTcc                                                                   >  1:464956/1‑147 (MQ=14)                                                                                       cgtaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑AACCCCCCCTACGTTGCCGTATACACA‑CTTTTCCGGCGGGCTCCTTCAGCCCCCCGGACCCCC‑CACCAAAATTTCGTTtctttctt                                                             >  1:730225/4‑150 (MQ=11)                                                                                       cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCtt                                                             <  1:358542/150‑1 (MQ=255)                                                                                       cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTg                                                            <  1:23008/151‑1 (MQ=255)                                                                                             aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCCCTACCTTGCCGTATACACA‑CTTTTCCCGCGTGCTCCTTCAGCCCCCCGGGCACCT‑CACCCAAATTTCGTTCCTGTCtt                                                             >  1:492526/1‑144 (MQ=14)                                                                                              aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAAc                                                     <  2:164160/151‑1 (MQ=255)                                                                                                aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑GGGGGGGGGTTG‑TACCCCCCCTAAGTTGGCGTATAAACA‑CCTTTCAGGCGGGGTCCTTCCGCCCCCCCGACACCC‑CCCCCAAATTTTGTTCCTTTCttttttgaa                                                      >  2:243445/1‑141 (MQ=2)                                                                                                  cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTCG‑AACCCCCCCTACGTTGCCGTATACACACCTTTCCA‑GCGTGCTCCTTCCGCCCCCCCGGCACCC‑CCCCCAAATTTCGGTTCTGTCTTTCTTGAACggg                                                  >  1:609260/1‑150 (MQ=9)                                                                                                  cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgc                                                 <  1:812806/151‑1 (MQ=255)                                                                                                  cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgc                                                 <  1:128021/151‑1 (MQ=255)                                                                                                    cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGTTTGTAACCCCCG‑CAAGTTGGCGTATAAAAA‑CTTTTCAAGGGGGCCCCTTCCGCCACCCCGGCTCCT‑CACCCAATTTTCGTTGCTTTCttttttggaggggcg                                                >  2:632816/1‑137 (MQ=2)                                                                                                       aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑GACCCCCCACACGTTGCCGTATAAACA‑CTTTTCAGGCGTGCTCCTTCAGCCACCCCGACACCT‑CACCAAAATGTTGTTTCTGTCTTTCTGGAACggggg                                                >  1:315217/1‑145 (MQ=14)                                                                                                       aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTa                                             <  1:699640/150‑1 (MQ=255)                                                                                                         tttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTG‑TACCCCCGCCACGTTGCCGGATACAAA‑CATTCCAGGCCGGCCCCTTTCGCCACCCCGAAACCC‑CCCCCAATTTTCGGTCCTCTCTTCTTGGGACGGgcgccga                                            >  1:1071785/1‑146 (MQ=2)                                                                                                           tCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCACACCTTGCCGGATACACA‑ATTTTCAGGCGTGGCCCTTCCGCCACTCGGACACCC‑CCCCCAAATTTCGTTCCTGTCTTGTTCGAACGGGCGCGCAttt                                         >  2:761525/1‑150 (MQ=9)                                                                                                            cGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTG‑GAACCCCCACACGTTGCTGTATACACA‑CATTTCCGGCGTGGTCCTTCTGCCCCCCCGGCACCC‑CCCCCAAATTTCGTTCCTGTCTTTCTGGGACGGGGGCCAAttt                                         >  2:315658/1‑149 (MQ=9)                                                                                                              tACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑ATCCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTa                                        <  2:902918/148‑1 (MQ=255)                                                                                                               aCGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAggg                                     <  1:191297/150‑1 (MQ=255)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTG‑GAACCCCCCTACCTTTCCCTATAAACA‑CCTTCCCAGCGCGGTCCCTTAGCCCCCCCGCCACCC‑CCCCCAAATTTTGTTCCTTTCTTTCTGGGAAAGGGGCCAAtttttggga                                   >  1:666701/1‑144 (MQ=2)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGGGG‑GGGGGGGGTTTG‑AACCCCCCGTACGGTGCCGTATACACA‑CTTTCCCGGCGTGCTCCTTCAGCCACCCCGGCACCC‑CACCCAATTGTCGTTTCTGTCTTTCTGGGAAGGGCGCTAATTTAGGGaa                                   >  1:921585/1‑150 (MQ=17)                                                                                                                      cttcttTAAATATGGCGGTGA‑GGGGGGGGTTTG‑AAACCCCCCTACGTTTCCGTATAAACA‑CCTTTCCGGCGGGCTCCTTCCGCCCCCCGGGCACCC‑CACCCAAATTTCGTTTCTGTCTTTCTGGAACGGGCGCCAAtttttgggaaaca                               >  1:108031/1‑139 (MQ=9)                                                                                                                      cttcttTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCa                               <  2:345548/149‑1 (MQ=255)                                                                                                                            tAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTg                        <  1:272868/150‑1 (MQ=255)                                                                                                                            tAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTg                        <  2:104705/150‑1 (MQ=255)                                                                                                                             aaaTATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGa                       <  1:1113392/150‑1 (MQ=255)                                                                                                                              aaTATGGCGGTGA‑GGGGGGGATTCC‑GACCCCCGATACGGTGCCGTATACACA‑CCTTCCAGGGGGGGGCCTTCAGCCACTCGGGCACCC‑CACCAAATTTTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCCGAgg                     >  2:932665/1‑151 (MQ=21)                                                                                                                                   ggCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaa                 <  1:748784/150‑1 (MQ=255)                                                                                                                                    gCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca               <  2:840221/151‑1 (MQ=255)                                                                                                                                    gCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca               <  2:848801/151‑1 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          <  1:1469051/151‑1 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          >  2:90188/1‑151 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          >  1:1382082/1‑151 (MQ=255)                                                                                                                                            gggggggATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt        >  2:1345985/1‑151 (MQ=255)                                                                                                                                               ggggATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGa     <  2:559156/151‑1 (MQ=255)                                                                                                                                               ggggATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGa     <  1:657937/151‑1 (MQ=255)                                                                                                                                                 ggATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaa   <  1:994789/151‑1 (MQ=255)                                                                                                                                                  gATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa  <  1:910251/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                         CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑